Development of an efficient process intensification strategy for enhancing Pfu DNA polymerase production in recombinant Escherichia coli

An efficient induction strategy that consisted of multiple additions of small doses of isopropyl-β-D-thiogalactopyranoside (IPTG) in the early cell growth phase was developed for enhancing Pfu DNA polymerase production in Escherichia coli . In comparison to the most commonly used method of a single...

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Veröffentlicht in:	Bioprocess and biosystems engineering 2015-04, Vol.38 (4), p.651-659
Hauptverfasser:	Hu, Jian-Hua, Wang, Feng, Liu, Chun-Zhao
Format:	Artikel
Sprache:	eng
Schlagworte:	Bioengineering Bioreactors Biotechnology Cell growth Chemistry Chemistry and Materials Science Deoxyribonucleic acid Dissolved oxygen DNA DNA polymerase DNA-Directed DNA Polymerase - biosynthesis E coli Environmental Engineering/Biotechnology Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Fermentation Food Science Gene Expression Regulation, Bacterial Industrial and Production Engineering Industrial Chemistry/Chemical Engineering Industrial Microbiology Isopropyl Thiogalactoside - chemistry Original Paper Oxygen - chemistry Pyrococcus furiosus - enzymology Recombinant Proteins - biosynthesis
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