Effect of cationic plastoquinone SkQ1 on electron transfer reactions in chloroplasts and mitochondria from pea seedlings
Plastoquinone bound with decyltriphenylphosphonium cation (SkQ1) penetrating through the membrane in nanomolar concentrations inhibited H 2 O 2 generation in cells of epidermis of pea seedling leaves that was detected by the fluorescence of 2′,7′-dichlorofluorescein. Photosynthetic electron transfer...
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| Veröffentlicht in: | Biochemistry (Moscow) 2015-04, Vol.80 (4), p.417-423 |
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| creator | Samuilov, V. D. Kiselevsky, D. B. |
| description | Plastoquinone bound with decyltriphenylphosphonium cation (SkQ1) penetrating through the membrane in nanomolar concentrations inhibited H
2
O
2
generation in cells of epidermis of pea seedling leaves that was detected by the fluorescence of 2′,7′-dichlorofluorescein. Photosynthetic electron transfer in chloroplasts isolated from pea leaves is suppressed by SkQ1 at micromolar concentrations: the electron transfer in chloroplasts under the action of photosystem II or I (with silicomolybdate or methyl viologen as electron acceptors, respectively) is more sensitive to SkQ1 than under the action of photosystem II + I (with ferricyanide or
p
-benzoquinone as electron acceptors). SkQ1 reduced by borohydride is oxidized by ferricyanide,
p
-benzoquinone, and, to a lesser extent, by silicomolybdate, but not by methyl viologen. SkQ1 is not effective as an electron acceptor supporting O
2
evolution from water in illuminated chloroplasts. The data on suppression of photosynthetic O
2
evolution or consumption show that SkQ1, similarly to phenazine methosulfate, causes conversion of the chloroplast redox-chain from non-cyclic electron transfer mode to the cyclic mode without O
2
evolution. Oxidation of NADH or succinate in mitochondria isolated from pea roots is stimulated by SkQ1. |
| doi_str_mv | 10.1134/S0006297915040045 |
| format | Article |
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2
O
2
generation in cells of epidermis of pea seedling leaves that was detected by the fluorescence of 2′,7′-dichlorofluorescein. Photosynthetic electron transfer in chloroplasts isolated from pea leaves is suppressed by SkQ1 at micromolar concentrations: the electron transfer in chloroplasts under the action of photosystem II or I (with silicomolybdate or methyl viologen as electron acceptors, respectively) is more sensitive to SkQ1 than under the action of photosystem II + I (with ferricyanide or
p
-benzoquinone as electron acceptors). SkQ1 reduced by borohydride is oxidized by ferricyanide,
p
-benzoquinone, and, to a lesser extent, by silicomolybdate, but not by methyl viologen. SkQ1 is not effective as an electron acceptor supporting O
2
evolution from water in illuminated chloroplasts. The data on suppression of photosynthetic O
2
evolution or consumption show that SkQ1, similarly to phenazine methosulfate, causes conversion of the chloroplast redox-chain from non-cyclic electron transfer mode to the cyclic mode without O
2
evolution. Oxidation of NADH or succinate in mitochondria isolated from pea roots is stimulated by SkQ1.</description><identifier>ISSN: 0006-2979</identifier><identifier>EISSN: 1608-3040</identifier><identifier>DOI: 10.1134/S0006297915040045</identifier><identifier>PMID: 25869358</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Bioorganic Chemistry ; Cell Respiration - drug effects ; Chloroplasts ; Chloroplasts - drug effects ; Chloroplasts - metabolism ; Electron transport ; Electron Transport - drug effects ; Electrons ; Epidermis ; Health aspects ; Hydrogen peroxide ; Leaves ; Life Sciences ; Microbiology ; Mitochondria ; Mitochondria - drug effects ; Mitochondria - metabolism ; Observations ; Peas ; Photosynthetic Reaction Center Complex Proteins - drug effects ; Pisum sativum - drug effects ; Pisum sativum - metabolism ; Plastoquinone - analogs & derivatives ; Plastoquinone - pharmacology ; Quinone ; Seedlings ; Seedlings - drug effects ; Seedlings - metabolism</subject><ispartof>Biochemistry (Moscow), 2015-04, Vol.80 (4), p.417-423</ispartof><rights>Pleiades Publishing, Ltd. 2015</rights><rights>COPYRIGHT 2015 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-9c9e5db495fe53aca307e686633c5b8c21657e6ffda2f1fcef645f6a42081a793</citedby><cites>FETCH-LOGICAL-c439t-9c9e5db495fe53aca307e686633c5b8c21657e6ffda2f1fcef645f6a42081a793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0006297915040045$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0006297915040045$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25869358$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Samuilov, V. D.</creatorcontrib><creatorcontrib>Kiselevsky, D. B.</creatorcontrib><title>Effect of cationic plastoquinone SkQ1 on electron transfer reactions in chloroplasts and mitochondria from pea seedlings</title><title>Biochemistry (Moscow)</title><addtitle>Biochemistry Moscow</addtitle><addtitle>Biochemistry (Mosc)</addtitle><description>Plastoquinone bound with decyltriphenylphosphonium cation (SkQ1) penetrating through the membrane in nanomolar concentrations inhibited H
2
O
2
generation in cells of epidermis of pea seedling leaves that was detected by the fluorescence of 2′,7′-dichlorofluorescein. Photosynthetic electron transfer in chloroplasts isolated from pea leaves is suppressed by SkQ1 at micromolar concentrations: the electron transfer in chloroplasts under the action of photosystem II or I (with silicomolybdate or methyl viologen as electron acceptors, respectively) is more sensitive to SkQ1 than under the action of photosystem II + I (with ferricyanide or
p
-benzoquinone as electron acceptors). SkQ1 reduced by borohydride is oxidized by ferricyanide,
p
-benzoquinone, and, to a lesser extent, by silicomolybdate, but not by methyl viologen. SkQ1 is not effective as an electron acceptor supporting O
2
evolution from water in illuminated chloroplasts. The data on suppression of photosynthetic O
2
evolution or consumption show that SkQ1, similarly to phenazine methosulfate, causes conversion of the chloroplast redox-chain from non-cyclic electron transfer mode to the cyclic mode without O
2
evolution. Oxidation of NADH or succinate in mitochondria isolated from pea roots is stimulated by SkQ1.</description><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bioorganic Chemistry</subject><subject>Cell Respiration - drug effects</subject><subject>Chloroplasts</subject><subject>Chloroplasts - drug effects</subject><subject>Chloroplasts - metabolism</subject><subject>Electron transport</subject><subject>Electron Transport - drug effects</subject><subject>Electrons</subject><subject>Epidermis</subject><subject>Health aspects</subject><subject>Hydrogen peroxide</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Mitochondria</subject><subject>Mitochondria - drug effects</subject><subject>Mitochondria - metabolism</subject><subject>Observations</subject><subject>Peas</subject><subject>Photosynthetic Reaction Center Complex Proteins - drug effects</subject><subject>Pisum sativum - drug effects</subject><subject>Pisum sativum - metabolism</subject><subject>Plastoquinone - analogs & derivatives</subject><subject>Plastoquinone - pharmacology</subject><subject>Quinone</subject><subject>Seedlings</subject><subject>Seedlings - drug effects</subject><subject>Seedlings - metabolism</subject><issn>0006-2979</issn><issn>1608-3040</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kU1P3DAQhq2qCLa0P6CXylIvXAL-dnxECGglpAoB58jrjBfTxN7aWan99zgstLQU-WDPzPOO5wOhj5QcUsrF0RUhRDGjDZVEECLkG7SgirQNr-ZbtJjDzRzfQ-9KuasmI4bvoj0mW2W4bBfo56n34CacPHZ2CikGh9eDLVP6sQkxRcBX3y8pThHDULlcH1O2sXjIOIN1s6TgELG7HVJOD9KCbezxGKbkblPsc7DY5zTiNVhcAPohxFV5j3a8HQp8eLz30c3Z6fXJl-bi2_nXk-OLxglupsY4A7JfCiM9SG6d5USDapXi3Mll6xhVsjq87y3z1DvwSkivrGCkpVYbvo8OtnnXubYEZerGUBwMg42QNqWjSnOutW5ZRT__g96lTY61ugeKCCa0_EOt7ABdiD7Vgbg5aXcsuNRGKjp_e_gfqp4exuDqXH2o_r8EdCtwOZWSwXfrHEabf3WUdPO2uxfbrppPjwVvliP0vxVP660A2wKlhuIK8rOOXs16DxbQs5s</recordid><startdate>20150401</startdate><enddate>20150401</enddate><creator>Samuilov, V. D.</creator><creator>Kiselevsky, D. B.</creator><general>Pleiades Publishing</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20150401</creationdate><title>Effect of cationic plastoquinone SkQ1 on electron transfer reactions in chloroplasts and mitochondria from pea seedlings</title><author>Samuilov, V. D. ; Kiselevsky, D. B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-9c9e5db495fe53aca307e686633c5b8c21657e6ffda2f1fcef645f6a42081a793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Bioorganic Chemistry</topic><topic>Cell Respiration - drug effects</topic><topic>Chloroplasts</topic><topic>Chloroplasts - drug effects</topic><topic>Chloroplasts - metabolism</topic><topic>Electron transport</topic><topic>Electron Transport - drug effects</topic><topic>Electrons</topic><topic>Epidermis</topic><topic>Health aspects</topic><topic>Hydrogen peroxide</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Mitochondria</topic><topic>Mitochondria - drug effects</topic><topic>Mitochondria - metabolism</topic><topic>Observations</topic><topic>Peas</topic><topic>Photosynthetic Reaction Center Complex Proteins - drug effects</topic><topic>Pisum sativum - drug effects</topic><topic>Pisum sativum - metabolism</topic><topic>Plastoquinone - analogs & derivatives</topic><topic>Plastoquinone - pharmacology</topic><topic>Quinone</topic><topic>Seedlings</topic><topic>Seedlings - drug effects</topic><topic>Seedlings - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Samuilov, V. D.</creatorcontrib><creatorcontrib>Kiselevsky, D. 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D.</au><au>Kiselevsky, D. B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of cationic plastoquinone SkQ1 on electron transfer reactions in chloroplasts and mitochondria from pea seedlings</atitle><jtitle>Biochemistry (Moscow)</jtitle><stitle>Biochemistry Moscow</stitle><addtitle>Biochemistry (Mosc)</addtitle><date>2015-04-01</date><risdate>2015</risdate><volume>80</volume><issue>4</issue><spage>417</spage><epage>423</epage><pages>417-423</pages><issn>0006-2979</issn><eissn>1608-3040</eissn><abstract>Plastoquinone bound with decyltriphenylphosphonium cation (SkQ1) penetrating through the membrane in nanomolar concentrations inhibited H
2
O
2
generation in cells of epidermis of pea seedling leaves that was detected by the fluorescence of 2′,7′-dichlorofluorescein. Photosynthetic electron transfer in chloroplasts isolated from pea leaves is suppressed by SkQ1 at micromolar concentrations: the electron transfer in chloroplasts under the action of photosystem II or I (with silicomolybdate or methyl viologen as electron acceptors, respectively) is more sensitive to SkQ1 than under the action of photosystem II + I (with ferricyanide or
p
-benzoquinone as electron acceptors). SkQ1 reduced by borohydride is oxidized by ferricyanide,
p
-benzoquinone, and, to a lesser extent, by silicomolybdate, but not by methyl viologen. SkQ1 is not effective as an electron acceptor supporting O
2
evolution from water in illuminated chloroplasts. The data on suppression of photosynthetic O
2
evolution or consumption show that SkQ1, similarly to phenazine methosulfate, causes conversion of the chloroplast redox-chain from non-cyclic electron transfer mode to the cyclic mode without O
2
evolution. Oxidation of NADH or succinate in mitochondria isolated from pea roots is stimulated by SkQ1.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><pmid>25869358</pmid><doi>10.1134/S0006297915040045</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-2979 |
| ispartof | Biochemistry (Moscow), 2015-04, Vol.80 (4), p.417-423 |
| issn | 0006-2979 1608-3040 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Biochemistry Biomedical and Life Sciences Biomedicine Bioorganic Chemistry Cell Respiration - drug effects Chloroplasts Chloroplasts - drug effects Chloroplasts - metabolism Electron transport Electron Transport - drug effects Electrons Epidermis Health aspects Hydrogen peroxide Leaves Life Sciences Microbiology Mitochondria Mitochondria - drug effects Mitochondria - metabolism Observations Peas Photosynthetic Reaction Center Complex Proteins - drug effects Pisum sativum - drug effects Pisum sativum - metabolism Plastoquinone - analogs & derivatives Plastoquinone - pharmacology Quinone Seedlings Seedlings - drug effects Seedlings - metabolism |
| title | Effect of cationic plastoquinone SkQ1 on electron transfer reactions in chloroplasts and mitochondria from pea seedlings |
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