The non-steroidal mycoestrogen zeranol suppresses luteinizing hormone secretion from the anterior pituitary of cattle via the estradiol receptor GPR30 in a rapid, non-genomic manner
Picomolar concentrations of estradiol produce rapid suppression of GnRH-induced luteinizing hormone (LH) secretion from the anterior pituitary (AP) of cattle via G-protein-coupled receptor 30 (GPR30). Zeranol is a strong estrogenic metabolite derived from zearalenone, a non-steroidal mycoestrogen pr...
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| Veröffentlicht in: | Animal reproduction science 2015-05, Vol.156, p.118-127 |
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| creator | Nakamura, Urara Rudolf, Faidiban O. Pandey, Kiran Kadokawa, Hiroya |
| description | Picomolar concentrations of estradiol produce rapid suppression of GnRH-induced luteinizing hormone (LH) secretion from the anterior pituitary (AP) of cattle via G-protein-coupled receptor 30 (GPR30). Zeranol is a strong estrogenic metabolite derived from zearalenone, a non-steroidal mycoestrogen produced by Fusarium that induces reproductive disorders in domestic animals. The hypothesis was tested that zeranol suppresses GnRH-induced LH release from the AP of cattle via GPR30 in a rapid, non-genomic manner. The AP cells (n=15) were cultured for 3 days in steroid-free conditions and then treated them with estradiol (0.001–10nM) or zeranol (0.001–100nM) for 5min before GnRH stimulation. Pre-treatment with 0.001–0.1nM estradiol suppressed GnRH-stimulated LH secretion. Pre-treatment with zeranol at concentrations of 0.001nM (P |
| doi_str_mv | 10.1016/j.anireprosci.2015.03.009 |
| format | Article |
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Zeranol is a strong estrogenic metabolite derived from zearalenone, a non-steroidal mycoestrogen produced by Fusarium that induces reproductive disorders in domestic animals. The hypothesis was tested that zeranol suppresses GnRH-induced LH release from the AP of cattle via GPR30 in a rapid, non-genomic manner. The AP cells (n=15) were cultured for 3 days in steroid-free conditions and then treated them with estradiol (0.001–10nM) or zeranol (0.001–100nM) for 5min before GnRH stimulation. Pre-treatment with 0.001–0.1nM estradiol suppressed GnRH-stimulated LH secretion. Pre-treatment with zeranol at concentrations of 0.001nM (P<0.01), 0.01nM (P<0.01), 0.1nM (P<0.05), and 1nM (P<0.05), but not at concentrations of 10 and 100nM, also inhibited GnRH-stimulated LH secretion from AP cells. Pre-treatment for 5min with a GPR30-specific antagonist, G36, inhibited estradiol or zeranol suppression of LH secretion from cultured AP cells. Cyclic AMP measurements and quantitative PCR analyses revealed that pre-treatment with small amounts of estradiol (P<0.05) or zeranol (P<0.01) decreased cAMP, but not gene expressions of the LHα, LHβ, or FSHβ subunits in the AP cells. Hence, zeranol may suppress luteinizing hormone secretion from the AP of cattle via GPR30 in a rapid, non-genomic manner.]]></description><identifier>ISSN: 0378-4320</identifier><identifier>EISSN: 1873-2232</identifier><identifier>DOI: 10.1016/j.anireprosci.2015.03.009</identifier><identifier>PMID: 25824341</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Benzodioxoles - pharmacology ; Cattle ; Cells, Cultured ; Cyclic AMP - physiology ; Estrogens, Non-Steroidal - pharmacology ; Female ; Fusarium ; G protein-coupled estrogen receptor-1 ; Gonadotrope ; Non-genomic effect ; Pituitary Gland, Anterior - drug effects ; Pituitary Gland, Anterior - physiology ; Quinolines - pharmacology ; Receptors, Estradiol - metabolism ; Ruminant ; Zearalenone ; Zeranol - pharmacology</subject><ispartof>Animal reproduction science, 2015-05, Vol.156, p.118-127</ispartof><rights>2015 Elsevier B.V.</rights><rights>Copyright © 2015 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c513t-7f09207749f587f6c3a499ec0c7f98a0d6f0740d3fc32be8081ce9693c7fadfc3</citedby><cites>FETCH-LOGICAL-c513t-7f09207749f587f6c3a499ec0c7f98a0d6f0740d3fc32be8081ce9693c7fadfc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.anireprosci.2015.03.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25824341$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nakamura, Urara</creatorcontrib><creatorcontrib>Rudolf, Faidiban O.</creatorcontrib><creatorcontrib>Pandey, Kiran</creatorcontrib><creatorcontrib>Kadokawa, Hiroya</creatorcontrib><title>The non-steroidal mycoestrogen zeranol suppresses luteinizing hormone secretion from the anterior pituitary of cattle via the estradiol receptor GPR30 in a rapid, non-genomic manner</title><title>Animal reproduction science</title><addtitle>Anim Reprod Sci</addtitle><description><![CDATA[Picomolar concentrations of estradiol produce rapid suppression of GnRH-induced luteinizing hormone (LH) secretion from the anterior pituitary (AP) of cattle via G-protein-coupled receptor 30 (GPR30). Zeranol is a strong estrogenic metabolite derived from zearalenone, a non-steroidal mycoestrogen produced by Fusarium that induces reproductive disorders in domestic animals. The hypothesis was tested that zeranol suppresses GnRH-induced LH release from the AP of cattle via GPR30 in a rapid, non-genomic manner. The AP cells (n=15) were cultured for 3 days in steroid-free conditions and then treated them with estradiol (0.001–10nM) or zeranol (0.001–100nM) for 5min before GnRH stimulation. Pre-treatment with 0.001–0.1nM estradiol suppressed GnRH-stimulated LH secretion. Pre-treatment with zeranol at concentrations of 0.001nM (P<0.01), 0.01nM (P<0.01), 0.1nM (P<0.05), and 1nM (P<0.05), but not at concentrations of 10 and 100nM, also inhibited GnRH-stimulated LH secretion from AP cells. Pre-treatment for 5min with a GPR30-specific antagonist, G36, inhibited estradiol or zeranol suppression of LH secretion from cultured AP cells. Cyclic AMP measurements and quantitative PCR analyses revealed that pre-treatment with small amounts of estradiol (P<0.05) or zeranol (P<0.01) decreased cAMP, but not gene expressions of the LHα, LHβ, or FSHβ subunits in the AP cells. Hence, zeranol may suppress luteinizing hormone secretion from the AP of cattle via GPR30 in a rapid, non-genomic manner.]]></description><subject>Animals</subject><subject>Benzodioxoles - pharmacology</subject><subject>Cattle</subject><subject>Cells, Cultured</subject><subject>Cyclic AMP - physiology</subject><subject>Estrogens, Non-Steroidal - pharmacology</subject><subject>Female</subject><subject>Fusarium</subject><subject>G protein-coupled estrogen receptor-1</subject><subject>Gonadotrope</subject><subject>Non-genomic effect</subject><subject>Pituitary Gland, Anterior - drug effects</subject><subject>Pituitary Gland, Anterior - physiology</subject><subject>Quinolines - pharmacology</subject><subject>Receptors, Estradiol - metabolism</subject><subject>Ruminant</subject><subject>Zearalenone</subject><subject>Zeranol - pharmacology</subject><issn>0378-4320</issn><issn>1873-2232</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFvFSEUhYnR2NfqXzC4c-E8LzAzDEvzoq1JE42pa0KZS8vLDIzANGn_l_9PXl81Ll2RwLnnnMtHyFsGWwas_7DfmuATLilm67ccWLcFsQVQz8iGDVI0nAv-nGxAyKFpBYcTcprzHgBk36uX5IR3A29Fyzbk19Ut0hBDkwum6Ecz0fneRswlxRsM9AGTCXGieV2WhDljptNa0Af_4MMNvY1pjgFpRpuw-BioS3GmpZqaUB19THTxZfXFpHsaHbWmlAnpnTePokOOGX0NSGhxKVV-_u27AOoDNTSZxY_vH-vVLnH2ls4mBEyvyAtnpoyvn84z8uPzp6vdRXP59fzL7uNlYzsmSiMdKA5Stsp1g3S9FaZVCi1Y6dRgYOwdyBZG4azg1zjAwCyqXon6bsZ6eUbeHX3rV_9ca1k9-2xxmkzAuGbNeimE7DveVak6Sm2lkhM6vSQ_1601A32gpvf6H2r6QE2D0JVanX3zFLNezzj-nfyDqQp2RwHWZe88Jl0tMFgcq6Eteoz-P2J-A9UYtGM</recordid><startdate>20150501</startdate><enddate>20150501</enddate><creator>Nakamura, Urara</creator><creator>Rudolf, Faidiban O.</creator><creator>Pandey, Kiran</creator><creator>Kadokawa, Hiroya</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150501</creationdate><title>The non-steroidal mycoestrogen zeranol suppresses luteinizing hormone secretion from the anterior pituitary of cattle via the estradiol receptor GPR30 in a rapid, non-genomic manner</title><author>Nakamura, Urara ; Rudolf, Faidiban O. ; Pandey, Kiran ; Kadokawa, Hiroya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c513t-7f09207749f587f6c3a499ec0c7f98a0d6f0740d3fc32be8081ce9693c7fadfc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Benzodioxoles - pharmacology</topic><topic>Cattle</topic><topic>Cells, Cultured</topic><topic>Cyclic AMP - physiology</topic><topic>Estrogens, Non-Steroidal - pharmacology</topic><topic>Female</topic><topic>Fusarium</topic><topic>G protein-coupled estrogen receptor-1</topic><topic>Gonadotrope</topic><topic>Non-genomic effect</topic><topic>Pituitary Gland, Anterior - drug effects</topic><topic>Pituitary Gland, Anterior - physiology</topic><topic>Quinolines - pharmacology</topic><topic>Receptors, Estradiol - metabolism</topic><topic>Ruminant</topic><topic>Zearalenone</topic><topic>Zeranol - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakamura, Urara</creatorcontrib><creatorcontrib>Rudolf, Faidiban O.</creatorcontrib><creatorcontrib>Pandey, Kiran</creatorcontrib><creatorcontrib>Kadokawa, Hiroya</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Animal reproduction science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakamura, Urara</au><au>Rudolf, Faidiban O.</au><au>Pandey, Kiran</au><au>Kadokawa, Hiroya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The non-steroidal mycoestrogen zeranol suppresses luteinizing hormone secretion from the anterior pituitary of cattle via the estradiol receptor GPR30 in a rapid, non-genomic manner</atitle><jtitle>Animal reproduction science</jtitle><addtitle>Anim Reprod Sci</addtitle><date>2015-05-01</date><risdate>2015</risdate><volume>156</volume><spage>118</spage><epage>127</epage><pages>118-127</pages><issn>0378-4320</issn><eissn>1873-2232</eissn><abstract><![CDATA[Picomolar concentrations of estradiol produce rapid suppression of GnRH-induced luteinizing hormone (LH) secretion from the anterior pituitary (AP) of cattle via G-protein-coupled receptor 30 (GPR30). Zeranol is a strong estrogenic metabolite derived from zearalenone, a non-steroidal mycoestrogen produced by Fusarium that induces reproductive disorders in domestic animals. The hypothesis was tested that zeranol suppresses GnRH-induced LH release from the AP of cattle via GPR30 in a rapid, non-genomic manner. The AP cells (n=15) were cultured for 3 days in steroid-free conditions and then treated them with estradiol (0.001–10nM) or zeranol (0.001–100nM) for 5min before GnRH stimulation. Pre-treatment with 0.001–0.1nM estradiol suppressed GnRH-stimulated LH secretion. Pre-treatment with zeranol at concentrations of 0.001nM (P<0.01), 0.01nM (P<0.01), 0.1nM (P<0.05), and 1nM (P<0.05), but not at concentrations of 10 and 100nM, also inhibited GnRH-stimulated LH secretion from AP cells. Pre-treatment for 5min with a GPR30-specific antagonist, G36, inhibited estradiol or zeranol suppression of LH secretion from cultured AP cells. Cyclic AMP measurements and quantitative PCR analyses revealed that pre-treatment with small amounts of estradiol (P<0.05) or zeranol (P<0.01) decreased cAMP, but not gene expressions of the LHα, LHβ, or FSHβ subunits in the AP cells. Hence, zeranol may suppress luteinizing hormone secretion from the AP of cattle via GPR30 in a rapid, non-genomic manner.]]></abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>25824341</pmid><doi>10.1016/j.anireprosci.2015.03.009</doi><tpages>10</tpages></addata></record> |
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| ispartof | Animal reproduction science, 2015-05, Vol.156, p.118-127 |
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| subjects | Animals Benzodioxoles - pharmacology Cattle Cells, Cultured Cyclic AMP - physiology Estrogens, Non-Steroidal - pharmacology Female Fusarium G protein-coupled estrogen receptor-1 Gonadotrope Non-genomic effect Pituitary Gland, Anterior - drug effects Pituitary Gland, Anterior - physiology Quinolines - pharmacology Receptors, Estradiol - metabolism Ruminant Zearalenone Zeranol - pharmacology |
| title | The non-steroidal mycoestrogen zeranol suppresses luteinizing hormone secretion from the anterior pituitary of cattle via the estradiol receptor GPR30 in a rapid, non-genomic manner |
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