Resveratrol attenuates inflammation and oxidative stress in epididymal white adipose tissue: Implications for its involvement in improving steroidogenesis in diet-induced obese mice
SUMMARY Chronic, low‐grade systemic inflammation has been shown to play an important role in the development of obesity‐related complications. Epididymal white adipose tissue (WAT) can influence testicular function through its endocrine function. The purpose of this study was to assess the effects o...
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| Veröffentlicht in: | Molecular reproduction and development 2015-04, Vol.82 (4), p.321-328 |
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| creator | Lv, Zheng-mei Wang, Qi Chen, Yuan-hua Wang, Sheng-hua Huang, Dao-qi |
| description | SUMMARY
Chronic, low‐grade systemic inflammation has been shown to play an important role in the development of obesity‐related complications. Epididymal white adipose tissue (WAT) can influence testicular function through its endocrine function. The purpose of this study was to assess the effects of resveratrol on the epididymal WAT inflammatory response and on testicular steroidogenesis in obese individuals. Seven‐week‐old male C57BL/6J mice were fed a high‐calorie and high‐cholesterol diet (HCD group) or HCD supplemented with resveratrol (HCD+Res group) for 18 weeks. As we previously showed that resveratrol protects against Leydig cell steroidogenesis in HCD‐induced obese mice, this study assessed macrophage infiltration in fat depots by measuring crown‐like structure (CLS) density. Histological analysis showed that adipocyte size was significantly smaller and CLSs were less numerous in the HCD+Res group than the HCD group (P |
| doi_str_mv | 10.1002/mrd.22478 |
| format | Article |
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Chronic, low‐grade systemic inflammation has been shown to play an important role in the development of obesity‐related complications. Epididymal white adipose tissue (WAT) can influence testicular function through its endocrine function. The purpose of this study was to assess the effects of resveratrol on the epididymal WAT inflammatory response and on testicular steroidogenesis in obese individuals. Seven‐week‐old male C57BL/6J mice were fed a high‐calorie and high‐cholesterol diet (HCD group) or HCD supplemented with resveratrol (HCD+Res group) for 18 weeks. As we previously showed that resveratrol protects against Leydig cell steroidogenesis in HCD‐induced obese mice, this study assessed macrophage infiltration in fat depots by measuring crown‐like structure (CLS) density. Histological analysis showed that adipocyte size was significantly smaller and CLSs were less numerous in the HCD+Res group than the HCD group (P < 0.01). Additionally, resveratrol supplementation decreased Nfkb1 expression (P < 0.01) and increased the IκB‐α protein abundance (P < 0.01) in epididymal WAT. Consistent with this alteration in NF‐κB signaling, the expression of two classic proinflammatory cytokines, TNF‐α (Tnfa) and IL‐1β (Il1b), were significantly decreased in the HCD+Res group compared with the HCD group (P < 0.01). Significant differences were also found in the expression of sirtuin1 (Sirt1) (P < 0.01) and manganese superoxide dismutase (Sod2) (P < 0.01) between the HCD and HCD+Res groups. Our data suggest that resveratrol can attenuate obesity‐induced inflammation and oxidative stress in epididymal WAT, which partly accounts for its beneficial effects in testicular steroidogenesis. Mol. Reprod. Dev. 82: 321–328, 2015. © 2015 Wiley Periodicals, Inc.]]></description><identifier>ISSN: 1040-452X</identifier><identifier>EISSN: 1098-2795</identifier><identifier>DOI: 10.1002/mrd.22478</identifier><identifier>PMID: 25820748</identifier><identifier>CODEN: MREDEE</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Adipose Tissue, White - drug effects ; Adipose Tissue, White - physiopathology ; Animals ; Blotting, Western ; DNA Primers - genetics ; Epididymis - cytology ; Epididymis - physiology ; Gonadal Steroid Hormones - biosynthesis ; Histological Techniques ; Immunohistochemistry ; Inflammation - drug therapy ; Male ; Mice ; Mice, Inbred C57BL ; NF-kappa B - metabolism ; Oxidative Stress - drug effects ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction - drug effects ; Stilbenes - pharmacology</subject><ispartof>Molecular reproduction and development, 2015-04, Vol.82 (4), p.321-328</ispartof><rights>2015 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3918-c6c3b12fbf615f29e8cf1590b47f8fcac57368e0fa3dfcd316d1dc1cae77f0a33</citedby><cites>FETCH-LOGICAL-c3918-c6c3b12fbf615f29e8cf1590b47f8fcac57368e0fa3dfcd316d1dc1cae77f0a33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmrd.22478$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmrd.22478$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25820748$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lv, Zheng-mei</creatorcontrib><creatorcontrib>Wang, Qi</creatorcontrib><creatorcontrib>Chen, Yuan-hua</creatorcontrib><creatorcontrib>Wang, Sheng-hua</creatorcontrib><creatorcontrib>Huang, Dao-qi</creatorcontrib><title>Resveratrol attenuates inflammation and oxidative stress in epididymal white adipose tissue: Implications for its involvement in improving steroidogenesis in diet-induced obese mice</title><title>Molecular reproduction and development</title><addtitle>Mol. Reprod. Dev</addtitle><description><![CDATA[SUMMARY
Chronic, low‐grade systemic inflammation has been shown to play an important role in the development of obesity‐related complications. Epididymal white adipose tissue (WAT) can influence testicular function through its endocrine function. The purpose of this study was to assess the effects of resveratrol on the epididymal WAT inflammatory response and on testicular steroidogenesis in obese individuals. Seven‐week‐old male C57BL/6J mice were fed a high‐calorie and high‐cholesterol diet (HCD group) or HCD supplemented with resveratrol (HCD+Res group) for 18 weeks. As we previously showed that resveratrol protects against Leydig cell steroidogenesis in HCD‐induced obese mice, this study assessed macrophage infiltration in fat depots by measuring crown‐like structure (CLS) density. Histological analysis showed that adipocyte size was significantly smaller and CLSs were less numerous in the HCD+Res group than the HCD group (P < 0.01). Additionally, resveratrol supplementation decreased Nfkb1 expression (P < 0.01) and increased the IκB‐α protein abundance (P < 0.01) in epididymal WAT. Consistent with this alteration in NF‐κB signaling, the expression of two classic proinflammatory cytokines, TNF‐α (Tnfa) and IL‐1β (Il1b), were significantly decreased in the HCD+Res group compared with the HCD group (P < 0.01). Significant differences were also found in the expression of sirtuin1 (Sirt1) (P < 0.01) and manganese superoxide dismutase (Sod2) (P < 0.01) between the HCD and HCD+Res groups. Our data suggest that resveratrol can attenuate obesity‐induced inflammation and oxidative stress in epididymal WAT, which partly accounts for its beneficial effects in testicular steroidogenesis. Mol. Reprod. Dev. 82: 321–328, 2015. © 2015 Wiley Periodicals, Inc.]]></description><subject>Adipose Tissue, White - drug effects</subject><subject>Adipose Tissue, White - physiopathology</subject><subject>Animals</subject><subject>Blotting, Western</subject><subject>DNA Primers - genetics</subject><subject>Epididymis - cytology</subject><subject>Epididymis - physiology</subject><subject>Gonadal Steroid Hormones - biosynthesis</subject><subject>Histological Techniques</subject><subject>Immunohistochemistry</subject><subject>Inflammation - drug therapy</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>NF-kappa B - metabolism</subject><subject>Oxidative Stress - drug effects</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Signal Transduction - drug effects</subject><subject>Stilbenes - pharmacology</subject><issn>1040-452X</issn><issn>1098-2795</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1vFSEUhidGY2t14R8wJG50MS0wH8y402prY9XkqtG4IVw41NMOwxWY294f5v-T6W27MHEFJM95eOEtiqeM7jNK-YELZp_zWnT3il1G-67kom_uz_ualnXDf-wUj2I8p5T2fUcfFju86TgVdbdb_FlAXENQKfiBqJRgnFSCSHC0g3JOJfQjUaMh_gpNPq2BxBQgzgSBFRo0G6cGcvkLExBlcOUjkIQxTvCKnLjVgPpaEon1gWCaB9d-WIODMc0SdKvg1zieZTEEj8afwQgRr28wCKnE0UwacoQlZLdDDY-LB1YNEZ7crHvFt6N3Xw_fl6efj08OX5-WuupZV-pWV0vG7dK2rLG8h05b1vR0WQvbWa10I6q2A2pVZaw2FWsNM5ppBUJYqqpqr3ix9eaIvyeISTqMGoZBjeCnKFkrqkrUTLCMPv8HPfdTGHO6meItE7zuMvVyS-ngYwxg5SqgU2EjGZVzlzJ3Ka-7zOyzG-O0dGDuyNvyMnCwBS5xgM3_TfLj4u2tstxOYP7rq7sJFS5kfolo5PdPx_JDz34eLcQb-aX6C0GxvhA</recordid><startdate>201504</startdate><enddate>201504</enddate><creator>Lv, Zheng-mei</creator><creator>Wang, Qi</creator><creator>Chen, Yuan-hua</creator><creator>Wang, Sheng-hua</creator><creator>Huang, Dao-qi</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201504</creationdate><title>Resveratrol attenuates inflammation and oxidative stress in epididymal white adipose tissue: Implications for its involvement in improving steroidogenesis in diet-induced obese mice</title><author>Lv, Zheng-mei ; Wang, Qi ; Chen, Yuan-hua ; Wang, Sheng-hua ; Huang, Dao-qi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3918-c6c3b12fbf615f29e8cf1590b47f8fcac57368e0fa3dfcd316d1dc1cae77f0a33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adipose Tissue, White - drug effects</topic><topic>Adipose Tissue, White - physiopathology</topic><topic>Animals</topic><topic>Blotting, Western</topic><topic>DNA Primers - genetics</topic><topic>Epididymis - cytology</topic><topic>Epididymis - physiology</topic><topic>Gonadal Steroid Hormones - biosynthesis</topic><topic>Histological Techniques</topic><topic>Immunohistochemistry</topic><topic>Inflammation - drug therapy</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>NF-kappa B - metabolism</topic><topic>Oxidative Stress - drug effects</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Signal Transduction - drug effects</topic><topic>Stilbenes - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lv, Zheng-mei</creatorcontrib><creatorcontrib>Wang, Qi</creatorcontrib><creatorcontrib>Chen, Yuan-hua</creatorcontrib><creatorcontrib>Wang, Sheng-hua</creatorcontrib><creatorcontrib>Huang, Dao-qi</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular reproduction and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lv, Zheng-mei</au><au>Wang, Qi</au><au>Chen, Yuan-hua</au><au>Wang, Sheng-hua</au><au>Huang, Dao-qi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Resveratrol attenuates inflammation and oxidative stress in epididymal white adipose tissue: Implications for its involvement in improving steroidogenesis in diet-induced obese mice</atitle><jtitle>Molecular reproduction and development</jtitle><addtitle>Mol. Reprod. Dev</addtitle><date>2015-04</date><risdate>2015</risdate><volume>82</volume><issue>4</issue><spage>321</spage><epage>328</epage><pages>321-328</pages><issn>1040-452X</issn><eissn>1098-2795</eissn><coden>MREDEE</coden><abstract><![CDATA[SUMMARY
Chronic, low‐grade systemic inflammation has been shown to play an important role in the development of obesity‐related complications. Epididymal white adipose tissue (WAT) can influence testicular function through its endocrine function. The purpose of this study was to assess the effects of resveratrol on the epididymal WAT inflammatory response and on testicular steroidogenesis in obese individuals. Seven‐week‐old male C57BL/6J mice were fed a high‐calorie and high‐cholesterol diet (HCD group) or HCD supplemented with resveratrol (HCD+Res group) for 18 weeks. As we previously showed that resveratrol protects against Leydig cell steroidogenesis in HCD‐induced obese mice, this study assessed macrophage infiltration in fat depots by measuring crown‐like structure (CLS) density. Histological analysis showed that adipocyte size was significantly smaller and CLSs were less numerous in the HCD+Res group than the HCD group (P < 0.01). Additionally, resveratrol supplementation decreased Nfkb1 expression (P < 0.01) and increased the IκB‐α protein abundance (P < 0.01) in epididymal WAT. Consistent with this alteration in NF‐κB signaling, the expression of two classic proinflammatory cytokines, TNF‐α (Tnfa) and IL‐1β (Il1b), were significantly decreased in the HCD+Res group compared with the HCD group (P < 0.01). Significant differences were also found in the expression of sirtuin1 (Sirt1) (P < 0.01) and manganese superoxide dismutase (Sod2) (P < 0.01) between the HCD and HCD+Res groups. Our data suggest that resveratrol can attenuate obesity‐induced inflammation and oxidative stress in epididymal WAT, which partly accounts for its beneficial effects in testicular steroidogenesis. Mol. Reprod. Dev. 82: 321–328, 2015. © 2015 Wiley Periodicals, Inc.]]></abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>25820748</pmid><doi>10.1002/mrd.22478</doi><tpages>8</tpages></addata></record> |
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| subjects | Adipose Tissue, White - drug effects Adipose Tissue, White - physiopathology Animals Blotting, Western DNA Primers - genetics Epididymis - cytology Epididymis - physiology Gonadal Steroid Hormones - biosynthesis Histological Techniques Immunohistochemistry Inflammation - drug therapy Male Mice Mice, Inbred C57BL NF-kappa B - metabolism Oxidative Stress - drug effects Reverse Transcriptase Polymerase Chain Reaction Signal Transduction - drug effects Stilbenes - pharmacology |
| title | Resveratrol attenuates inflammation and oxidative stress in epididymal white adipose tissue: Implications for its involvement in improving steroidogenesis in diet-induced obese mice |
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