Hyaluronic acid nanogels with enzyme-sensitive cross-linking group for drug delivery
A methacrylation strategy was employed to functionalize hyaluronic acid and prepare hyaluronic acid (HA) nanogels. Dynamic light scattering, zeta potential analyzer and electron microscopy were utilized to characterize the nanogels and their enzyme-degradability in vitro. It was found that these nan...
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| Veröffentlicht in: | Journal of controlled release 2015-05, Vol.205, p.206-217 |
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| creator | Yang, Chenchen Wang, Xin Yao, Xikuang Zhang, Yajun Wu, Wei Jiang, Xiqun |
| description | A methacrylation strategy was employed to functionalize hyaluronic acid and prepare hyaluronic acid (HA) nanogels. Dynamic light scattering, zeta potential analyzer and electron microscopy were utilized to characterize the nanogels and their enzyme-degradability in vitro. It was found that these nanogels had a spherical morphology with the diameter of about 70nm, and negative surface potential. When doxorubicin (DOX) was loaded into the nanogels, the diameter decreased to approximately 50nm with a drug loading content of 16% and encapsulation efficiency of 62%. Cellular uptake examinations showed that HA nanogels could be preferentially internalized by two-dimensional (2D) cells and three-dimensional (3D) multicellular spheroids (MCs) which both overexpress CD44 receptor. Near-infrared fluorescence imaging, biodistribution and penetration examinations in tumor tissue indicated that the HA nanogels could efficiently accumulate and penetrate the tumor matrix. In vivo antitumor evaluation found that DOX-loaded HA nanogels exhibited a significantly superior antitumor effect.
Doxorubicin-loaded hyaluronic acid nanogels were synthesized by a methacrylated strategy. In vitro cellular uptake shows that these nanogels were preferentially internalized by the CD44 or CD168-overexpressed cancer cells. In vivo antitumor examination indicates that these nanogels suppress tumor growth distinctly. [Display omitted] |
| doi_str_mv | 10.1016/j.jconrel.2015.02.008 |
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Doxorubicin-loaded hyaluronic acid nanogels were synthesized by a methacrylated strategy. In vitro cellular uptake shows that these nanogels were preferentially internalized by the CD44 or CD168-overexpressed cancer cells. In vivo antitumor examination indicates that these nanogels suppress tumor growth distinctly. [Display omitted]</description><identifier>ISSN: 0168-3659</identifier><identifier>EISSN: 1873-4995</identifier><identifier>DOI: 10.1016/j.jconrel.2015.02.008</identifier><identifier>PMID: 25665867</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antibiotics, Antineoplastic - chemistry ; Antibiotics, Antineoplastic - metabolism ; Antibiotics, Antineoplastic - pharmacology ; Cell Survival - drug effects ; Chemistry, Pharmaceutical ; Dose-Response Relationship, Drug ; Doxorubicin - chemistry ; Doxorubicin - metabolism ; Doxorubicin - pharmacology ; Drug Carriers ; Drug delivery ; Gels ; Hep G2 Cells ; Humans ; Hyaluronan Receptors - metabolism ; Hyaluronic Acid - analogs & derivatives ; Hyaluronic Acid - chemistry ; Hyaluronic Acid - metabolism ; Hyaluronoglucosaminidase - chemistry ; Hyaluronoglucosaminidase - metabolism ; Light ; Lipase - chemistry ; Lipase - metabolism ; Male ; Mice ; Mice, Inbred ICR ; Microscopy, Electron, Transmission ; Multicellular spheroids ; Nanogel hyaluronic acid ; Nanomedicine ; Nanoparticles ; NIH 3T3 Cells ; Particle Size ; Scattering, Radiation ; Solubility ; Spectroscopy, Fourier Transform Infrared ; Spheroids, Cellular ; Surface Properties ; Technology, Pharmaceutical - methods ; Time Factors</subject><ispartof>Journal of controlled release, 2015-05, Vol.205, p.206-217</ispartof><rights>2015 Elsevier B.V.</rights><rights>Copyright © 2015 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c435t-e73110b43e7438c3bd1b1ab7fbafb80c7903501582d55691dac4f672cd562f123</citedby><cites>FETCH-LOGICAL-c435t-e73110b43e7438c3bd1b1ab7fbafb80c7903501582d55691dac4f672cd562f123</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S016836591500098X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25665867$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Chenchen</creatorcontrib><creatorcontrib>Wang, Xin</creatorcontrib><creatorcontrib>Yao, Xikuang</creatorcontrib><creatorcontrib>Zhang, Yajun</creatorcontrib><creatorcontrib>Wu, Wei</creatorcontrib><creatorcontrib>Jiang, Xiqun</creatorcontrib><title>Hyaluronic acid nanogels with enzyme-sensitive cross-linking group for drug delivery</title><title>Journal of controlled release</title><addtitle>J Control Release</addtitle><description>A methacrylation strategy was employed to functionalize hyaluronic acid and prepare hyaluronic acid (HA) nanogels. Dynamic light scattering, zeta potential analyzer and electron microscopy were utilized to characterize the nanogels and their enzyme-degradability in vitro. It was found that these nanogels had a spherical morphology with the diameter of about 70nm, and negative surface potential. When doxorubicin (DOX) was loaded into the nanogels, the diameter decreased to approximately 50nm with a drug loading content of 16% and encapsulation efficiency of 62%. Cellular uptake examinations showed that HA nanogels could be preferentially internalized by two-dimensional (2D) cells and three-dimensional (3D) multicellular spheroids (MCs) which both overexpress CD44 receptor. Near-infrared fluorescence imaging, biodistribution and penetration examinations in tumor tissue indicated that the HA nanogels could efficiently accumulate and penetrate the tumor matrix. In vivo antitumor evaluation found that DOX-loaded HA nanogels exhibited a significantly superior antitumor effect.
Doxorubicin-loaded hyaluronic acid nanogels were synthesized by a methacrylated strategy. In vitro cellular uptake shows that these nanogels were preferentially internalized by the CD44 or CD168-overexpressed cancer cells. In vivo antitumor examination indicates that these nanogels suppress tumor growth distinctly. [Display omitted]</description><subject>Animals</subject><subject>Antibiotics, Antineoplastic - chemistry</subject><subject>Antibiotics, Antineoplastic - metabolism</subject><subject>Antibiotics, Antineoplastic - pharmacology</subject><subject>Cell Survival - drug effects</subject><subject>Chemistry, Pharmaceutical</subject><subject>Dose-Response Relationship, Drug</subject><subject>Doxorubicin - chemistry</subject><subject>Doxorubicin - metabolism</subject><subject>Doxorubicin - pharmacology</subject><subject>Drug Carriers</subject><subject>Drug delivery</subject><subject>Gels</subject><subject>Hep G2 Cells</subject><subject>Humans</subject><subject>Hyaluronan Receptors - metabolism</subject><subject>Hyaluronic Acid - analogs & derivatives</subject><subject>Hyaluronic Acid - chemistry</subject><subject>Hyaluronic Acid - metabolism</subject><subject>Hyaluronoglucosaminidase - chemistry</subject><subject>Hyaluronoglucosaminidase - metabolism</subject><subject>Light</subject><subject>Lipase - chemistry</subject><subject>Lipase - metabolism</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred ICR</subject><subject>Microscopy, Electron, Transmission</subject><subject>Multicellular spheroids</subject><subject>Nanogel hyaluronic acid</subject><subject>Nanomedicine</subject><subject>Nanoparticles</subject><subject>NIH 3T3 Cells</subject><subject>Particle Size</subject><subject>Scattering, Radiation</subject><subject>Solubility</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Spheroids, Cellular</subject><subject>Surface Properties</subject><subject>Technology, Pharmaceutical - methods</subject><subject>Time Factors</subject><issn>0168-3659</issn><issn>1873-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFO4zAURS0EGkqZTxjkJZtk7Di2kxVCiIGRKrEpa8uxX4o7qV3spKjz9WNoYTurtznvXt2D0A9KSkqo-Lku1yb4CENZEcpLUpWENCdoRhvJirpt-SmaZa4pmODtObpIaU0I4ayW39B5xYXgjZAztHzc62GKwTuDtXEWe-3DCoaE39z4gsH_3W-gSOCTG90OsIkhpWJw_o_zK7yKYdriPkRs47TCFobMxP0lOuv1kOD78c7R86_75d1jsXh6-H13uyhMzfhYgGSUkq5mIGvWGNZZ2lHdyb7TfdcQI1vCeB7XVJZz0VKrTd0LWRnLRdXTis3R9SF3G8PrBGlUG5cMDIP2EKakqJCM5GxKMsoP6MeACL3aRrfRca8oUe9C1Vodhap3oYpUKgvNf1fHiqnbgP36-jSYgZsDkJ3BzkFUyTjwBqyLYEZlg_tPxT-0noqu</recordid><startdate>20150510</startdate><enddate>20150510</enddate><creator>Yang, Chenchen</creator><creator>Wang, Xin</creator><creator>Yao, Xikuang</creator><creator>Zhang, Yajun</creator><creator>Wu, Wei</creator><creator>Jiang, Xiqun</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150510</creationdate><title>Hyaluronic acid nanogels with enzyme-sensitive cross-linking group for drug delivery</title><author>Yang, Chenchen ; Wang, Xin ; Yao, Xikuang ; Zhang, Yajun ; Wu, Wei ; Jiang, Xiqun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-e73110b43e7438c3bd1b1ab7fbafb80c7903501582d55691dac4f672cd562f123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Antibiotics, Antineoplastic - chemistry</topic><topic>Antibiotics, Antineoplastic - metabolism</topic><topic>Antibiotics, Antineoplastic - pharmacology</topic><topic>Cell Survival - drug effects</topic><topic>Chemistry, Pharmaceutical</topic><topic>Dose-Response Relationship, Drug</topic><topic>Doxorubicin - chemistry</topic><topic>Doxorubicin - metabolism</topic><topic>Doxorubicin - pharmacology</topic><topic>Drug Carriers</topic><topic>Drug delivery</topic><topic>Gels</topic><topic>Hep G2 Cells</topic><topic>Humans</topic><topic>Hyaluronan Receptors - metabolism</topic><topic>Hyaluronic Acid - analogs & derivatives</topic><topic>Hyaluronic Acid - chemistry</topic><topic>Hyaluronic Acid - metabolism</topic><topic>Hyaluronoglucosaminidase - chemistry</topic><topic>Hyaluronoglucosaminidase - metabolism</topic><topic>Light</topic><topic>Lipase - chemistry</topic><topic>Lipase - metabolism</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred ICR</topic><topic>Microscopy, Electron, Transmission</topic><topic>Multicellular spheroids</topic><topic>Nanogel hyaluronic acid</topic><topic>Nanomedicine</topic><topic>Nanoparticles</topic><topic>NIH 3T3 Cells</topic><topic>Particle Size</topic><topic>Scattering, Radiation</topic><topic>Solubility</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Spheroids, Cellular</topic><topic>Surface Properties</topic><topic>Technology, Pharmaceutical - methods</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Chenchen</creatorcontrib><creatorcontrib>Wang, Xin</creatorcontrib><creatorcontrib>Yao, Xikuang</creatorcontrib><creatorcontrib>Zhang, Yajun</creatorcontrib><creatorcontrib>Wu, Wei</creatorcontrib><creatorcontrib>Jiang, Xiqun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of controlled release</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Chenchen</au><au>Wang, Xin</au><au>Yao, Xikuang</au><au>Zhang, Yajun</au><au>Wu, Wei</au><au>Jiang, Xiqun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hyaluronic acid nanogels with enzyme-sensitive cross-linking group for drug delivery</atitle><jtitle>Journal of controlled release</jtitle><addtitle>J Control Release</addtitle><date>2015-05-10</date><risdate>2015</risdate><volume>205</volume><spage>206</spage><epage>217</epage><pages>206-217</pages><issn>0168-3659</issn><eissn>1873-4995</eissn><abstract>A methacrylation strategy was employed to functionalize hyaluronic acid and prepare hyaluronic acid (HA) nanogels. Dynamic light scattering, zeta potential analyzer and electron microscopy were utilized to characterize the nanogels and their enzyme-degradability in vitro. It was found that these nanogels had a spherical morphology with the diameter of about 70nm, and negative surface potential. When doxorubicin (DOX) was loaded into the nanogels, the diameter decreased to approximately 50nm with a drug loading content of 16% and encapsulation efficiency of 62%. Cellular uptake examinations showed that HA nanogels could be preferentially internalized by two-dimensional (2D) cells and three-dimensional (3D) multicellular spheroids (MCs) which both overexpress CD44 receptor. Near-infrared fluorescence imaging, biodistribution and penetration examinations in tumor tissue indicated that the HA nanogels could efficiently accumulate and penetrate the tumor matrix. In vivo antitumor evaluation found that DOX-loaded HA nanogels exhibited a significantly superior antitumor effect.
Doxorubicin-loaded hyaluronic acid nanogels were synthesized by a methacrylated strategy. In vitro cellular uptake shows that these nanogels were preferentially internalized by the CD44 or CD168-overexpressed cancer cells. In vivo antitumor examination indicates that these nanogels suppress tumor growth distinctly. [Display omitted]</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>25665867</pmid><doi>10.1016/j.jconrel.2015.02.008</doi><tpages>12</tpages></addata></record> |
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| subjects | Animals Antibiotics, Antineoplastic - chemistry Antibiotics, Antineoplastic - metabolism Antibiotics, Antineoplastic - pharmacology Cell Survival - drug effects Chemistry, Pharmaceutical Dose-Response Relationship, Drug Doxorubicin - chemistry Doxorubicin - metabolism Doxorubicin - pharmacology Drug Carriers Drug delivery Gels Hep G2 Cells Humans Hyaluronan Receptors - metabolism Hyaluronic Acid - analogs & derivatives Hyaluronic Acid - chemistry Hyaluronic Acid - metabolism Hyaluronoglucosaminidase - chemistry Hyaluronoglucosaminidase - metabolism Light Lipase - chemistry Lipase - metabolism Male Mice Mice, Inbred ICR Microscopy, Electron, Transmission Multicellular spheroids Nanogel hyaluronic acid Nanomedicine Nanoparticles NIH 3T3 Cells Particle Size Scattering, Radiation Solubility Spectroscopy, Fourier Transform Infrared Spheroids, Cellular Surface Properties Technology, Pharmaceutical - methods Time Factors |
| title | Hyaluronic acid nanogels with enzyme-sensitive cross-linking group for drug delivery |
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