Water as a competitive inhibitor of lipase-catalysed esterification in organic media

Kinetic parameters were determined for esterification of dodecanol and decanoic acid in hexane catalysed by lipases from Rhizomucor miehei and Candida rugosa, after pre-equilibration to different values of thermodynamic water activity (aw). Vm increases with increasing aw, but so do the Km values fo...

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Veröffentlicht in:	Biotechnology letters 1993-11, Vol.15 (11), p.1133-1138
Hauptverfasser:	Valivety, R.H, Halling, P.J, Macrae, A.R
Format:	Artikel
Sprache:	eng
Schlagworte:	1-dodecanol binding sites Biological and medical sciences Biotechnology Candida rugosa decanoic acid Enzyme engineering esterification Fundamental and applied biological sciences. Psychology metabolic inhibitors Methods. Procedures. Technologies Miscellaneous Rhizomucor miehei triacylglycerol lipase water content
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