Cotton (Gossypium hirsutum L.) pollen-specific polygalacturonase mRNA: tissue and temporal specificity of its promoter in transgenic tobacco
A gene (G9) expressed during late microsporogenesis in cotton (Gossypium hirsutum L.) was isolated. Sequence analysis of the cDNA (1.3 kb) as well as the gene (2.6 kb) revealed an open reading frame of 1233 bases encoding a protein of 43.9 kDa. The coding region of the gene is interrupted by three i...
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| Veröffentlicht in: | Plant molecular biology 1994-12, Vol.26 (6), p.1989-1993 |
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| container_end_page | 1993 |
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| container_issue | 6 |
| container_start_page | 1989 |
| container_title | Plant molecular biology |
| container_volume | 26 |
| creator | John, M.E. (Agracetus Inc., Middleton, WI (USA)) Petersen, M.W |
| description | A gene (G9) expressed during late microsporogenesis in cotton (Gossypium hirsutum L.) was isolated. Sequence analysis of the cDNA (1.3 kb) as well as the gene (2.6 kb) revealed an open reading frame of 1233 bases encoding a protein of 43.9 kDa. The coding region of the gene is interrupted by three introns. Northern analysis of the RNA from developing anthers showed that the transcripts appear 12 days before anthesis and that the maximal concentration of RNA occurs in pollen on the day of anthesis. This pattern of gene expression suggests functions in post-anthesis events. Sequence comparisons with other known plant genes indicated that G9 is homologous to polygalacturonases. The G9 promoter conferred tissue and temporal specificity of beta-glucuronidase (GUS) expression in transgenic tobacco plants. Thus, the G9 promoter can be used to drive gene expression in homologous as well as heterologous plants in a tissue-specific manner. |
| doi_str_mv | 10.1007/bf00019509 |
| format | Article |
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(Agracetus Inc., Middleton, WI (USA)) ; Petersen, M.W</creator><creatorcontrib>John, M.E. (Agracetus Inc., Middleton, WI (USA)) ; Petersen, M.W</creatorcontrib><description>A gene (G9) expressed during late microsporogenesis in cotton (Gossypium hirsutum L.) was isolated. Sequence analysis of the cDNA (1.3 kb) as well as the gene (2.6 kb) revealed an open reading frame of 1233 bases encoding a protein of 43.9 kDa. The coding region of the gene is interrupted by three introns. Northern analysis of the RNA from developing anthers showed that the transcripts appear 12 days before anthesis and that the maximal concentration of RNA occurs in pollen on the day of anthesis. This pattern of gene expression suggests functions in post-anthesis events. Sequence comparisons with other known plant genes indicated that G9 is homologous to polygalacturonases. The G9 promoter conferred tissue and temporal specificity of beta-glucuronidase (GUS) expression in transgenic tobacco plants. Thus, the G9 promoter can be used to drive gene expression in homologous as well as heterologous plants in a tissue-specific manner.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1007/bf00019509</identifier><identifier>PMID: 7858233</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Amino Acid Sequence ; ARN MENSAJERO ; ARN MESSAGER ; DNA, Complementary - genetics ; EXPRESION GENICA ; EXPRESSION DES GENES ; GENE EXPRESSION ; Genes, Plant - genetics ; GENETIC TRANSFORMATION ; Genome, Plant ; Gossypium - enzymology ; Gossypium - genetics ; Gossypium - growth & development ; GOSSYPIUM HIRSUTUM ; MESSENGER RNA ; Molecular Sequence Data ; NICOTIANA ; Nicotiana - genetics ; PLANTAS TRANSGENICAS ; PLANTE TRANSGENIQUE ; Plants, Genetically Modified ; Plants, Toxic ; POLIGALACTURONASA ; Pollen - enzymology ; Pollen - genetics ; POLYGALACTURONASE ; Polygalacturonase - genetics ; Promoter Regions, Genetic - genetics ; RNA, Messenger - analysis ; Time Factors ; Tissue Distribution ; TRANSFORMACION GENETICA ; TRANSFORMATION GENETIQUE ; TRANSGENIC PLANTS</subject><ispartof>Plant molecular biology, 1994-12, Vol.26 (6), p.1989-1993</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c342t-322fddf46c848f29be87ba7a1983ea0581e2ea93ecf9076ffcce1e38c098893a3</citedby><cites>FETCH-LOGICAL-c342t-322fddf46c848f29be87ba7a1983ea0581e2ea93ecf9076ffcce1e38c098893a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7858233$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>John, M.E. (Agracetus Inc., Middleton, WI (USA))</creatorcontrib><creatorcontrib>Petersen, M.W</creatorcontrib><title>Cotton (Gossypium hirsutum L.) pollen-specific polygalacturonase mRNA: tissue and temporal specificity of its promoter in transgenic tobacco</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>A gene (G9) expressed during late microsporogenesis in cotton (Gossypium hirsutum L.) was isolated. Sequence analysis of the cDNA (1.3 kb) as well as the gene (2.6 kb) revealed an open reading frame of 1233 bases encoding a protein of 43.9 kDa. The coding region of the gene is interrupted by three introns. Northern analysis of the RNA from developing anthers showed that the transcripts appear 12 days before anthesis and that the maximal concentration of RNA occurs in pollen on the day of anthesis. This pattern of gene expression suggests functions in post-anthesis events. Sequence comparisons with other known plant genes indicated that G9 is homologous to polygalacturonases. The G9 promoter conferred tissue and temporal specificity of beta-glucuronidase (GUS) expression in transgenic tobacco plants. Thus, the G9 promoter can be used to drive gene expression in homologous as well as heterologous plants in a tissue-specific manner.</description><subject>Amino Acid Sequence</subject><subject>ARN MENSAJERO</subject><subject>ARN MESSAGER</subject><subject>DNA, Complementary - genetics</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>GENE EXPRESSION</subject><subject>Genes, Plant - genetics</subject><subject>GENETIC TRANSFORMATION</subject><subject>Genome, Plant</subject><subject>Gossypium - enzymology</subject><subject>Gossypium - genetics</subject><subject>Gossypium - growth & development</subject><subject>GOSSYPIUM HIRSUTUM</subject><subject>MESSENGER RNA</subject><subject>Molecular Sequence Data</subject><subject>NICOTIANA</subject><subject>Nicotiana - genetics</subject><subject>PLANTAS TRANSGENICAS</subject><subject>PLANTE TRANSGENIQUE</subject><subject>Plants, Genetically Modified</subject><subject>Plants, Toxic</subject><subject>POLIGALACTURONASA</subject><subject>Pollen - enzymology</subject><subject>Pollen - genetics</subject><subject>POLYGALACTURONASE</subject><subject>Polygalacturonase - genetics</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>RNA, Messenger - analysis</subject><subject>Time Factors</subject><subject>Tissue Distribution</subject><subject>TRANSFORMACION GENETICA</subject><subject>TRANSFORMATION GENETIQUE</subject><subject>TRANSGENIC PLANTS</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kV1LHDEUhkOx2FV708uCkCvRwmg-5iPxThe1wmJB7PVwJnuyTZmZjEnmYv9Df7SRXXt1cnifvIQnhHzj7JIz1lx1ljHGdcX0J7LgVSOLigl1QBaM101Rllx8IUcx_s0QY7I-JIeNqpSQckH-LX1KfqTnDz7G7eTmgf5xIc4pH1aXF3TyfY9jESc0zjrzvm830INJc_AjRKTD89PNNU0uxhkpjGuacJh8gJ5-XHJpS72lLkU6BT_4hIG6kaYAY9zgmFuT78AYf0I-W-gjft3PY_L7_u5l-bNY_Xp4XN6sCiNLkQophF2vbVkbVSordIeq6aABrpVEYJXiKBC0RGM1a2prjUGOUhmmldIS5DE52_Xm57zOGFM7uGiw72FEP8c2WxN1VcoM_tiBJmQ9AW07BTdA2Lacte_q29v7D_UZPt23zt2A6__o3nXOv-9yC76FTXCxfVrpOv-RruUbyhKJkw</recordid><startdate>199412</startdate><enddate>199412</enddate><creator>John, M.E. (Agracetus Inc., Middleton, WI (USA))</creator><creator>Petersen, M.W</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>199412</creationdate><title>Cotton (Gossypium hirsutum L.) pollen-specific polygalacturonase mRNA: tissue and temporal specificity of its promoter in transgenic tobacco</title><author>John, M.E. (Agracetus Inc., Middleton, WI (USA)) ; Petersen, M.W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c342t-322fddf46c848f29be87ba7a1983ea0581e2ea93ecf9076ffcce1e38c098893a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Amino Acid Sequence</topic><topic>ARN MENSAJERO</topic><topic>ARN MESSAGER</topic><topic>DNA, Complementary - genetics</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>GENE EXPRESSION</topic><topic>Genes, Plant - genetics</topic><topic>GENETIC TRANSFORMATION</topic><topic>Genome, Plant</topic><topic>Gossypium - enzymology</topic><topic>Gossypium - genetics</topic><topic>Gossypium - growth & development</topic><topic>GOSSYPIUM HIRSUTUM</topic><topic>MESSENGER RNA</topic><topic>Molecular Sequence Data</topic><topic>NICOTIANA</topic><topic>Nicotiana - genetics</topic><topic>PLANTAS TRANSGENICAS</topic><topic>PLANTE TRANSGENIQUE</topic><topic>Plants, Genetically Modified</topic><topic>Plants, Toxic</topic><topic>POLIGALACTURONASA</topic><topic>Pollen - enzymology</topic><topic>Pollen - genetics</topic><topic>POLYGALACTURONASE</topic><topic>Polygalacturonase - genetics</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>RNA, Messenger - analysis</topic><topic>Time Factors</topic><topic>Tissue Distribution</topic><topic>TRANSFORMACION GENETICA</topic><topic>TRANSFORMATION GENETIQUE</topic><topic>TRANSGENIC PLANTS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>John, M.E. (Agracetus Inc., Middleton, WI (USA))</creatorcontrib><creatorcontrib>Petersen, M.W</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>John, M.E. (Agracetus Inc., Middleton, WI (USA))</au><au>Petersen, M.W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cotton (Gossypium hirsutum L.) pollen-specific polygalacturonase mRNA: tissue and temporal specificity of its promoter in transgenic tobacco</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1994-12</date><risdate>1994</risdate><volume>26</volume><issue>6</issue><spage>1989</spage><epage>1993</epage><pages>1989-1993</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><abstract>A gene (G9) expressed during late microsporogenesis in cotton (Gossypium hirsutum L.) was isolated. Sequence analysis of the cDNA (1.3 kb) as well as the gene (2.6 kb) revealed an open reading frame of 1233 bases encoding a protein of 43.9 kDa. The coding region of the gene is interrupted by three introns. Northern analysis of the RNA from developing anthers showed that the transcripts appear 12 days before anthesis and that the maximal concentration of RNA occurs in pollen on the day of anthesis. This pattern of gene expression suggests functions in post-anthesis events. Sequence comparisons with other known plant genes indicated that G9 is homologous to polygalacturonases. The G9 promoter conferred tissue and temporal specificity of beta-glucuronidase (GUS) expression in transgenic tobacco plants. Thus, the G9 promoter can be used to drive gene expression in homologous as well as heterologous plants in a tissue-specific manner.</abstract><cop>Netherlands</cop><pmid>7858233</pmid><doi>10.1007/bf00019509</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Plant molecular biology, 1994-12, Vol.26 (6), p.1989-1993 |
| issn | 0167-4412 1573-5028 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Amino Acid Sequence ARN MENSAJERO ARN MESSAGER DNA, Complementary - genetics EXPRESION GENICA EXPRESSION DES GENES GENE EXPRESSION Genes, Plant - genetics GENETIC TRANSFORMATION Genome, Plant Gossypium - enzymology Gossypium - genetics Gossypium - growth & development GOSSYPIUM HIRSUTUM MESSENGER RNA Molecular Sequence Data NICOTIANA Nicotiana - genetics PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE Plants, Genetically Modified Plants, Toxic POLIGALACTURONASA Pollen - enzymology Pollen - genetics POLYGALACTURONASE Polygalacturonase - genetics Promoter Regions, Genetic - genetics RNA, Messenger - analysis Time Factors Tissue Distribution TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE TRANSGENIC PLANTS |
| title | Cotton (Gossypium hirsutum L.) pollen-specific polygalacturonase mRNA: tissue and temporal specificity of its promoter in transgenic tobacco |
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