Mono- and polyclonal antibodies to the organophosphate fenitrothion. 1. Approaches to hapten-protein conjugation
Three different chemical approaches were used to couple the organophosphorus pesticide fenitrothion to carrier proteins for production of polyclonal and monoclonal antibodies. Hapten conjugates coupled through the pesticide phosphate group yielded the most specific and highest affinity antibodies, a...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 1992-08, Vol.40 (8), p.1466-1470 |
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| container_end_page | 1470 |
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| container_issue | 8 |
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| container_title | Journal of agricultural and food chemistry |
| container_volume | 40 |
| creator | McAdam, David P Hill, Amanda S Beasley, Helen L Skerritt, John H |
| description | Three different chemical approaches were used to couple the organophosphorus pesticide fenitrothion to carrier proteins for production of polyclonal and monoclonal antibodies. Hapten conjugates coupled through the pesticide phosphate group yielded the most specific and highest affinity antibodies, although antibodies of moderate affinity were obtained by derivatization and coupling through the aromatic nitro group following its reduction and amide formation with an adipic acid spacer arm. Assay formats using either immobilized antibody or immobilized hapten-protein conjugate could be used to determine free fenitrothion. The most sensitive assay could detect 1 ng of fenitrothion |
| doi_str_mv | 10.1021/jf00020a035 |
| format | Article |
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The most sensitive assay could detect 1 ng of fenitrothion</description><subject>ANALISIS CUANTITATIVO</subject><subject>ANALYSE QUANTITATIVE</subject><subject>ANTICORPS</subject><subject>ANTICORPS MONOCLONAL</subject><subject>ANTICUERPOS</subject><subject>ANTICUERPOS MONOCLONALES</subject><subject>ELISA</subject><subject>FENITOTRION</subject><subject>FENITROTHION</subject><subject>HAPTENE</subject><subject>HAPTENOS</subject><subject>TEST ELISA</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNptkE1P3DAQhi3USl2gp9568qkcUBZPEifOkS4UKlFBBajcrFnH3ngJdrC9Uvn3NQqqeuhpNPM-8_US8gnYElgJJ1vDGCsZsorvkQXwkhUcQLwji1yGQvAGPpD9GLcZE7xlCzL98M4XFF1PJz--qNE7HHOa7Nr3VkeaPE2Dpj5s0Plp8HEaMGlqtLMp-DRY75YUlvR0moJHNcwtA05JuyKXkraOKu-2uw2mDB-S9wbHqD--xQNy_-38bnVZXF1ffF-dXhVYVXUq1muohGoqYdpOY_6p4XU-mTHNVdcZ1tW16k2rGm6wB-i16FGA6vu24mXTQHVAvsxz8w3POx2TfLJR6XFEp_0uSmhaEDV0GTyeQRV8jEEbOQX7hOFFApOvrsp_XM10MdM2Jv37L4rhUTZt1XJ5d3MrH1Zn5c-zy1_ya-Y_z7xBL3ETbJT3t12dfxQsi0eziCrKrd-F7H3879o_naGPPA</recordid><startdate>19920801</startdate><enddate>19920801</enddate><creator>McAdam, David P</creator><creator>Hill, Amanda S</creator><creator>Beasley, Helen L</creator><creator>Skerritt, John H</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>19920801</creationdate><title>Mono- and polyclonal antibodies to the organophosphate fenitrothion. 1. 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Approaches to hapten-protein conjugation</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>1992-08-01</date><risdate>1992</risdate><volume>40</volume><issue>8</issue><spage>1466</spage><epage>1470</epage><pages>1466-1470</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><abstract>Three different chemical approaches were used to couple the organophosphorus pesticide fenitrothion to carrier proteins for production of polyclonal and monoclonal antibodies. Hapten conjugates coupled through the pesticide phosphate group yielded the most specific and highest affinity antibodies, although antibodies of moderate affinity were obtained by derivatization and coupling through the aromatic nitro group following its reduction and amide formation with an adipic acid spacer arm. Assay formats using either immobilized antibody or immobilized hapten-protein conjugate could be used to determine free fenitrothion. 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| fulltext | fulltext |
| identifier | ISSN: 0021-8561 |
| ispartof | Journal of agricultural and food chemistry, 1992-08, Vol.40 (8), p.1466-1470 |
| issn | 0021-8561 1520-5118 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16718419 |
| source | ACS Publications |
| subjects | ANALISIS CUANTITATIVO ANALYSE QUANTITATIVE ANTICORPS ANTICORPS MONOCLONAL ANTICUERPOS ANTICUERPOS MONOCLONALES ELISA FENITOTRION FENITROTHION HAPTENE HAPTENOS TEST ELISA |
| title | Mono- and polyclonal antibodies to the organophosphate fenitrothion. 1. Approaches to hapten-protein conjugation |
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