Evaluation of DNA lesions caused by high-LET radiation using the polymerase chain reaction
Generally, it is thought that the bio-effect by the radiation depends on LET. Compared to low-LET radiation, High-LET radiation induces a large number of single and double stranded breaks. Therefore, it is important that radiation exposure of the different radiation of LET is monitored. In this pape...
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| Veröffentlicht in: | Radiation measurements 2013-08, Vol.55, p.93-95 |
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| container_title | Radiation measurements |
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| creator | Matuo, Youichirou Izumi, Yoshinobu Sato, Norihito Yamamoto, Takayoshi Shimizu, Kikuo |
| description | Generally, it is thought that the bio-effect by the radiation depends on LET. Compared to low-LET radiation, High-LET radiation induces a large number of single and double stranded breaks. Therefore, it is important that radiation exposure of the different radiation of LET is monitored. In this paper, the evaluation methods of DNA lesions caused by high-LET radiation using the polymerase chain reaction are descried. We found that the efficiency of DNA synthesis decreased with increasing LET through experiment of the analysis of the DNA lesion by the polymerase chain reaction. In addition, it was confirmed about this method has the potential to be used to simultaneously evaluate the biological effects caused by high-LET radiation.
► The evaluation methods of DNA lesions caused by high-LET radiation using the polymerase chain reaction are presented. ► The efficiency of DNA synthesis decreased with increasing LET. ► The method presented here is useful to evaluate the biological effects by various types of radiation with different LET. |
| doi_str_mv | 10.1016/j.radmeas.2013.01.016 |
| format | Article |
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► The evaluation methods of DNA lesions caused by high-LET radiation using the polymerase chain reaction are presented. ► The efficiency of DNA synthesis decreased with increasing LET. ► The method presented here is useful to evaluate the biological effects by various types of radiation with different LET.</description><subject>Biodosimetry</subject><subject>Biological effects</subject><subject>Breaking</subject><subject>Deoxyribonucleic acid</subject><subject>DNA damages</subject><subject>Earth sciences</subject><subject>Earth, ocean, space</subject><subject>Exact sciences and technology</subject><subject>Geochronology</subject><subject>Isotope geochemistry. 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► The evaluation methods of DNA lesions caused by high-LET radiation using the polymerase chain reaction are presented. ► The efficiency of DNA synthesis decreased with increasing LET. ► The method presented here is useful to evaluate the biological effects by various types of radiation with different LET.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.radmeas.2013.01.016</doi><tpages>3</tpages></addata></record> |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Biodosimetry Biological effects Breaking Deoxyribonucleic acid DNA damages Earth sciences Earth, ocean, space Exact sciences and technology Geochronology Isotope geochemistry. Geochronology Lesions LET Polymerase chain reaction Radiation measurement Synthesis |
| title | Evaluation of DNA lesions caused by high-LET radiation using the polymerase chain reaction |
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