Electrochemical deoxyribonucleic acid biosensor based on carboxyl functionalized graphene oxide and poly-l-lysine modified electrode for the detection of tlh gene sequence related to vibrio parahaemolyticus
[Display omitted] ► Ploy-l-lysine modified glassy carbon electrode was prepared by electropolymerization. ► A carboxyl functionalized graphene oxide was synthesized and decorated on PLLy/GCE. ► Electrochemical DNA biosensor based on GO-COOH/PLLy/GCE was fabricated. ► The tlh gene related to vibrio p...
Gespeichert in:
| Veröffentlicht in: | Analytica chimica acta 2012-11, Vol.752, p.39-44 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 44 |
|---|---|
| container_issue | |
| container_start_page | 39 |
| container_title | Analytica chimica acta |
| container_volume | 752 |
| creator | Sun, Wei Zhang, Yuanyuan Ju, Xiaomei Li, Guangjiu Gao, Hongwei Sun, Zhenfan |
| description | [Display omitted]
► Ploy-l-lysine modified glassy carbon electrode was prepared by electropolymerization. ► A carboxyl functionalized graphene oxide was synthesized and decorated on PLLy/GCE. ► Electrochemical DNA biosensor based on GO-COOH/PLLy/GCE was fabricated. ► The tlh gene related to vibrio parahaemolyticus was detected successfully.
A carboxyl functionalized graphene oxide (GO-COOH) and electropolymerized ploy-l-lysine (PLLy) modified glassy carbon electrode (GCE) was fabricated and used for the construction of an electrochemical deoxyribonucleic acid (DNA) biosensor. The NH2 modified probe ssDNA sequences were immobilized on the surface of GO-COOH/PLLy/GCE by covalent linking with the formation of amide bonds, which was stable and furthur hybridized with the target ssDNA sequence. Differential pulse voltammetry (DPV) was used to monitor the hybridization events with methylene blue as electrochemical indicator, which gave a sensitive reduction peak at −0.287V (vs. SCE). Under the optimal conditions the reduction peak current was proportional to the concentration of tlh gene sequence in the range from 1.0×10−12 to 1.0×10−6molL−1 with a detection limit as 1.69×10−13molL−1 (3σ). The polymerase chain reaction products of tlh gene from oyster samples were detected with satisfactory results, indicating the potential application of this electrochemical DNA sensor. |
| doi_str_mv | 10.1016/j.aca.2012.09.009 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1671614803</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0003267012013360</els_id><sourcerecordid>1125252088</sourcerecordid><originalsourceid>FETCH-LOGICAL-c449t-f0698952c148756514a1b495e1c5c88c3676f4055b3e946e56a312a22515d9903</originalsourceid><addsrcrecordid>eNqFks1u1DAUhSMEokPhAdggb5DYZPD_xGKFqvIjVWIDa8txbpo7cuLBTqoOD8kz4TAD7Ki8sGx_59wr31NVLxndMsr02_3WebfllPEtNVtKzaNqw5qdqKXg8nG1oZSKmusdvaie5bwvR86ofFpdcLHqFd1UP68D-DlFP8CI3gXSQbw_JmzjtPgA6Inz2JEWY4Ypx0Ral6EjcSLepbaggfTL5GeMkwv4ozzdJncYYAIS77ED4qaOHGI41qEOx4zlfowd9lhIOJUuUF-M5wFK8Rl-e5HYkzkM5HY1yvB9gckDSRDcXIRzJHfYJozk4JIbHIylwIx-yc-rJ70LGV6c98vq24frr1ef6psvHz9fvb-pvZRmrnuqTWMU90w2O6UVk4610ihgXvmm8ULvdC-pUq0AIzUo7QTjjnPFVGcMFZfVm5PvIcXSXJ7tiNlDCG6CuGTL9I7pYk7Fw6iQjWyMEOphlHFVFm2agrIT6lPMOUFvDwlHl46WUbsO1-5tCYddw2GpsSUcRfPqbL-0I3R_FX_SUIDXZ8DlkoU-uclj_sdptVKr0bsTB-WL7xCSzR7XCXWYyvxsF_E_bfwCbZXaVQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1125252088</pqid></control><display><type>article</type><title>Electrochemical deoxyribonucleic acid biosensor based on carboxyl functionalized graphene oxide and poly-l-lysine modified electrode for the detection of tlh gene sequence related to vibrio parahaemolyticus</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Sun, Wei ; Zhang, Yuanyuan ; Ju, Xiaomei ; Li, Guangjiu ; Gao, Hongwei ; Sun, Zhenfan</creator><creatorcontrib>Sun, Wei ; Zhang, Yuanyuan ; Ju, Xiaomei ; Li, Guangjiu ; Gao, Hongwei ; Sun, Zhenfan</creatorcontrib><description>[Display omitted]
► Ploy-l-lysine modified glassy carbon electrode was prepared by electropolymerization. ► A carboxyl functionalized graphene oxide was synthesized and decorated on PLLy/GCE. ► Electrochemical DNA biosensor based on GO-COOH/PLLy/GCE was fabricated. ► The tlh gene related to vibrio parahaemolyticus was detected successfully.
A carboxyl functionalized graphene oxide (GO-COOH) and electropolymerized ploy-l-lysine (PLLy) modified glassy carbon electrode (GCE) was fabricated and used for the construction of an electrochemical deoxyribonucleic acid (DNA) biosensor. The NH2 modified probe ssDNA sequences were immobilized on the surface of GO-COOH/PLLy/GCE by covalent linking with the formation of amide bonds, which was stable and furthur hybridized with the target ssDNA sequence. Differential pulse voltammetry (DPV) was used to monitor the hybridization events with methylene blue as electrochemical indicator, which gave a sensitive reduction peak at −0.287V (vs. SCE). Under the optimal conditions the reduction peak current was proportional to the concentration of tlh gene sequence in the range from 1.0×10−12 to 1.0×10−6molL−1 with a detection limit as 1.69×10−13molL−1 (3σ). The polymerase chain reaction products of tlh gene from oyster samples were detected with satisfactory results, indicating the potential application of this electrochemical DNA sensor.</description><identifier>ISSN: 0003-2670</identifier><identifier>EISSN: 1873-4324</identifier><identifier>DOI: 10.1016/j.aca.2012.09.009</identifier><identifier>PMID: 23101650</identifier><identifier>CODEN: ACACAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Analytical chemistry ; Biological and medical sciences ; Biosensing Techniques ; Biosensors ; Biotechnology ; Carboxyl functionalized graphene oxide ; Chemistry ; Deoxyribonucleic acid ; DNA - chemistry ; DNA, Single-Stranded - chemistry ; DNA, Single-Stranded - genetics ; Electrochemical deoxyribonucleic acid biosensor ; Electrochemical methods ; Electrochemical Techniques ; Electrodes ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; General, instrumentation ; Genes ; Glassy carbon electrode ; Graphene ; Graphite - chemistry ; Methods. Procedures. Technologies ; Monitors ; Oxides ; Oxides - chemistry ; Ploy-l-lysine ; Polylysine - chemistry ; Polymerase Chain Reaction ; Reduction ; Various methods and equipments ; Vibrio parahaemolyticus ; Vibrio parahaemolyticus - genetics</subject><ispartof>Analytica chimica acta, 2012-11, Vol.752, p.39-44</ispartof><rights>2012 Elsevier B.V.</rights><rights>2014 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c449t-f0698952c148756514a1b495e1c5c88c3676f4055b3e946e56a312a22515d9903</citedby><cites>FETCH-LOGICAL-c449t-f0698952c148756514a1b495e1c5c88c3676f4055b3e946e56a312a22515d9903</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0003267012013360$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26565039$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23101650$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sun, Wei</creatorcontrib><creatorcontrib>Zhang, Yuanyuan</creatorcontrib><creatorcontrib>Ju, Xiaomei</creatorcontrib><creatorcontrib>Li, Guangjiu</creatorcontrib><creatorcontrib>Gao, Hongwei</creatorcontrib><creatorcontrib>Sun, Zhenfan</creatorcontrib><title>Electrochemical deoxyribonucleic acid biosensor based on carboxyl functionalized graphene oxide and poly-l-lysine modified electrode for the detection of tlh gene sequence related to vibrio parahaemolyticus</title><title>Analytica chimica acta</title><addtitle>Anal Chim Acta</addtitle><description>[Display omitted]
► Ploy-l-lysine modified glassy carbon electrode was prepared by electropolymerization. ► A carboxyl functionalized graphene oxide was synthesized and decorated on PLLy/GCE. ► Electrochemical DNA biosensor based on GO-COOH/PLLy/GCE was fabricated. ► The tlh gene related to vibrio parahaemolyticus was detected successfully.
A carboxyl functionalized graphene oxide (GO-COOH) and electropolymerized ploy-l-lysine (PLLy) modified glassy carbon electrode (GCE) was fabricated and used for the construction of an electrochemical deoxyribonucleic acid (DNA) biosensor. The NH2 modified probe ssDNA sequences were immobilized on the surface of GO-COOH/PLLy/GCE by covalent linking with the formation of amide bonds, which was stable and furthur hybridized with the target ssDNA sequence. Differential pulse voltammetry (DPV) was used to monitor the hybridization events with methylene blue as electrochemical indicator, which gave a sensitive reduction peak at −0.287V (vs. SCE). Under the optimal conditions the reduction peak current was proportional to the concentration of tlh gene sequence in the range from 1.0×10−12 to 1.0×10−6molL−1 with a detection limit as 1.69×10−13molL−1 (3σ). The polymerase chain reaction products of tlh gene from oyster samples were detected with satisfactory results, indicating the potential application of this electrochemical DNA sensor.</description><subject>Amino Acid Sequence</subject><subject>Analytical chemistry</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Carboxyl functionalized graphene oxide</subject><subject>Chemistry</subject><subject>Deoxyribonucleic acid</subject><subject>DNA - chemistry</subject><subject>DNA, Single-Stranded - chemistry</subject><subject>DNA, Single-Stranded - genetics</subject><subject>Electrochemical deoxyribonucleic acid biosensor</subject><subject>Electrochemical methods</subject><subject>Electrochemical Techniques</subject><subject>Electrodes</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General, instrumentation</subject><subject>Genes</subject><subject>Glassy carbon electrode</subject><subject>Graphene</subject><subject>Graphite - chemistry</subject><subject>Methods. Procedures. Technologies</subject><subject>Monitors</subject><subject>Oxides</subject><subject>Oxides - chemistry</subject><subject>Ploy-l-lysine</subject><subject>Polylysine - chemistry</subject><subject>Polymerase Chain Reaction</subject><subject>Reduction</subject><subject>Various methods and equipments</subject><subject>Vibrio parahaemolyticus</subject><subject>Vibrio parahaemolyticus - genetics</subject><issn>0003-2670</issn><issn>1873-4324</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks1u1DAUhSMEokPhAdggb5DYZPD_xGKFqvIjVWIDa8txbpo7cuLBTqoOD8kz4TAD7Ki8sGx_59wr31NVLxndMsr02_3WebfllPEtNVtKzaNqw5qdqKXg8nG1oZSKmusdvaie5bwvR86ofFpdcLHqFd1UP68D-DlFP8CI3gXSQbw_JmzjtPgA6Inz2JEWY4Ypx0Ral6EjcSLepbaggfTL5GeMkwv4ozzdJncYYAIS77ED4qaOHGI41qEOx4zlfowd9lhIOJUuUF-M5wFK8Rl-e5HYkzkM5HY1yvB9gckDSRDcXIRzJHfYJozk4JIbHIylwIx-yc-rJ70LGV6c98vq24frr1ef6psvHz9fvb-pvZRmrnuqTWMU90w2O6UVk4610ihgXvmm8ULvdC-pUq0AIzUo7QTjjnPFVGcMFZfVm5PvIcXSXJ7tiNlDCG6CuGTL9I7pYk7Fw6iQjWyMEOphlHFVFm2agrIT6lPMOUFvDwlHl46WUbsO1-5tCYddw2GpsSUcRfPqbL-0I3R_FX_SUIDXZ8DlkoU-uclj_sdptVKr0bsTB-WL7xCSzR7XCXWYyvxsF_E_bfwCbZXaVQ</recordid><startdate>20121108</startdate><enddate>20121108</enddate><creator>Sun, Wei</creator><creator>Zhang, Yuanyuan</creator><creator>Ju, Xiaomei</creator><creator>Li, Guangjiu</creator><creator>Gao, Hongwei</creator><creator>Sun, Zhenfan</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7QO</scope><scope>7TM</scope><scope>7TN</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope><scope>7U5</scope><scope>L7M</scope></search><sort><creationdate>20121108</creationdate><title>Electrochemical deoxyribonucleic acid biosensor based on carboxyl functionalized graphene oxide and poly-l-lysine modified electrode for the detection of tlh gene sequence related to vibrio parahaemolyticus</title><author>Sun, Wei ; Zhang, Yuanyuan ; Ju, Xiaomei ; Li, Guangjiu ; Gao, Hongwei ; Sun, Zhenfan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-f0698952c148756514a1b495e1c5c88c3676f4055b3e946e56a312a22515d9903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Amino Acid Sequence</topic><topic>Analytical chemistry</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Carboxyl functionalized graphene oxide</topic><topic>Chemistry</topic><topic>Deoxyribonucleic acid</topic><topic>DNA - chemistry</topic><topic>DNA, Single-Stranded - chemistry</topic><topic>DNA, Single-Stranded - genetics</topic><topic>Electrochemical deoxyribonucleic acid biosensor</topic><topic>Electrochemical methods</topic><topic>Electrochemical Techniques</topic><topic>Electrodes</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General, instrumentation</topic><topic>Genes</topic><topic>Glassy carbon electrode</topic><topic>Graphene</topic><topic>Graphite - chemistry</topic><topic>Methods. Procedures. Technologies</topic><topic>Monitors</topic><topic>Oxides</topic><topic>Oxides - chemistry</topic><topic>Ploy-l-lysine</topic><topic>Polylysine - chemistry</topic><topic>Polymerase Chain Reaction</topic><topic>Reduction</topic><topic>Various methods and equipments</topic><topic>Vibrio parahaemolyticus</topic><topic>Vibrio parahaemolyticus - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sun, Wei</creatorcontrib><creatorcontrib>Zhang, Yuanyuan</creatorcontrib><creatorcontrib>Ju, Xiaomei</creatorcontrib><creatorcontrib>Li, Guangjiu</creatorcontrib><creatorcontrib>Gao, Hongwei</creatorcontrib><creatorcontrib>Sun, Zhenfan</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Analytica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, Wei</au><au>Zhang, Yuanyuan</au><au>Ju, Xiaomei</au><au>Li, Guangjiu</au><au>Gao, Hongwei</au><au>Sun, Zhenfan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrochemical deoxyribonucleic acid biosensor based on carboxyl functionalized graphene oxide and poly-l-lysine modified electrode for the detection of tlh gene sequence related to vibrio parahaemolyticus</atitle><jtitle>Analytica chimica acta</jtitle><addtitle>Anal Chim Acta</addtitle><date>2012-11-08</date><risdate>2012</risdate><volume>752</volume><spage>39</spage><epage>44</epage><pages>39-44</pages><issn>0003-2670</issn><eissn>1873-4324</eissn><coden>ACACAM</coden><abstract>[Display omitted]
► Ploy-l-lysine modified glassy carbon electrode was prepared by electropolymerization. ► A carboxyl functionalized graphene oxide was synthesized and decorated on PLLy/GCE. ► Electrochemical DNA biosensor based on GO-COOH/PLLy/GCE was fabricated. ► The tlh gene related to vibrio parahaemolyticus was detected successfully.
A carboxyl functionalized graphene oxide (GO-COOH) and electropolymerized ploy-l-lysine (PLLy) modified glassy carbon electrode (GCE) was fabricated and used for the construction of an electrochemical deoxyribonucleic acid (DNA) biosensor. The NH2 modified probe ssDNA sequences were immobilized on the surface of GO-COOH/PLLy/GCE by covalent linking with the formation of amide bonds, which was stable and furthur hybridized with the target ssDNA sequence. Differential pulse voltammetry (DPV) was used to monitor the hybridization events with methylene blue as electrochemical indicator, which gave a sensitive reduction peak at −0.287V (vs. SCE). Under the optimal conditions the reduction peak current was proportional to the concentration of tlh gene sequence in the range from 1.0×10−12 to 1.0×10−6molL−1 with a detection limit as 1.69×10−13molL−1 (3σ). The polymerase chain reaction products of tlh gene from oyster samples were detected with satisfactory results, indicating the potential application of this electrochemical DNA sensor.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>23101650</pmid><doi>10.1016/j.aca.2012.09.009</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2670 |
| ispartof | Analytica chimica acta, 2012-11, Vol.752, p.39-44 |
| issn | 0003-2670 1873-4324 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1671614803 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Analytical chemistry Biological and medical sciences Biosensing Techniques Biosensors Biotechnology Carboxyl functionalized graphene oxide Chemistry Deoxyribonucleic acid DNA - chemistry DNA, Single-Stranded - chemistry DNA, Single-Stranded - genetics Electrochemical deoxyribonucleic acid biosensor Electrochemical methods Electrochemical Techniques Electrodes Exact sciences and technology Fundamental and applied biological sciences. Psychology General, instrumentation Genes Glassy carbon electrode Graphene Graphite - chemistry Methods. Procedures. Technologies Monitors Oxides Oxides - chemistry Ploy-l-lysine Polylysine - chemistry Polymerase Chain Reaction Reduction Various methods and equipments Vibrio parahaemolyticus Vibrio parahaemolyticus - genetics |
| title | Electrochemical deoxyribonucleic acid biosensor based on carboxyl functionalized graphene oxide and poly-l-lysine modified electrode for the detection of tlh gene sequence related to vibrio parahaemolyticus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T00%3A11%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Electrochemical%20deoxyribonucleic%20acid%20biosensor%20based%20on%20carboxyl%20functionalized%20graphene%20oxide%20and%20poly-l-lysine%20modified%20electrode%20for%20the%20detection%20of%20tlh%20gene%20sequence%20related%20to%20vibrio%20parahaemolyticus&rft.jtitle=Analytica%20chimica%20acta&rft.au=Sun,%20Wei&rft.date=2012-11-08&rft.volume=752&rft.spage=39&rft.epage=44&rft.pages=39-44&rft.issn=0003-2670&rft.eissn=1873-4324&rft.coden=ACACAM&rft_id=info:doi/10.1016/j.aca.2012.09.009&rft_dat=%3Cproquest_cross%3E1125252088%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1125252088&rft_id=info:pmid/23101650&rft_els_id=S0003267012013360&rfr_iscdi=true |