Direct ethanol production from cellulosic materials by Zymobacter palmae carrying Cellulomonas endoglucanase and Ruminococcus β-glucosidase genes
In order to reduce the cost of bioethanol production from lignocellulosic biomass, we conferred the ability to ferment cellulosic materials directly on Zymobacter palmae by co-expressing foreign endoglucanase and β-glucosidase genes. Z . palmae is a novel ethanol-fermenting bacterium capable of util...
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creator | Kojima, Motoki Okamoto, Kenji Yanase, Hideshi |
description | In order to reduce the cost of bioethanol production from lignocellulosic biomass, we conferred the ability to ferment cellulosic materials directly on
Zymobacter palmae
by co-expressing foreign endoglucanase and β-glucosidase genes.
Z
.
palmae
is a novel ethanol-fermenting bacterium capable of utilizing a broad range of sugar substrates, but not cellulose. Therefore, the six genes encoding the cellulolytic enzymes (CenA, CenB, CenD, CbhA, CbhB, and Cex) from
Cellulomonas fimi
were introduced and expressed in
Z
.
palmae
. Of these cellulolytic enzyme genes cloned, CenA degraded carboxymethylcellulose and phosphoric acid-swollen cellulose (PASC) efficiently. The extracellular CenA catalyzed the hydrolysis of barley β-glucan and PASC to liberate soluble cello-oligosaccharides, indicating that CenA is the most suitable enzyme for cellulose degradation among those cellulolytic enzymes expressed in
Z
.
palmae
. Furthermore, the
cenA
gene and β-glucosidase gene (
bgl
) from
Ruminococcus albus
were co-expressed in
Z
.
palmae
. Of the total endoglucanase and β-glucosidase activities, 57.1 and 18.1 % were localized in the culture medium of the strain. The genetically engineered strain completely saccharified and fermented 20 g/l barley β-glucan to ethanol within 84 h, producing 79.5 % of the theoretical yield. Thus, the production and secretion of CenA and BGL enabled
Z
.
palmae
to efficiently ferment a water-soluble cellulosic polysaccharide to ethanol. |
doi_str_mv | 10.1007/s00253-013-4874-1 |
format | Article |
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Zymobacter palmae
by co-expressing foreign endoglucanase and β-glucosidase genes.
Z
.
palmae
is a novel ethanol-fermenting bacterium capable of utilizing a broad range of sugar substrates, but not cellulose. Therefore, the six genes encoding the cellulolytic enzymes (CenA, CenB, CenD, CbhA, CbhB, and Cex) from
Cellulomonas fimi
were introduced and expressed in
Z
.
palmae
. Of these cellulolytic enzyme genes cloned, CenA degraded carboxymethylcellulose and phosphoric acid-swollen cellulose (PASC) efficiently. The extracellular CenA catalyzed the hydrolysis of barley β-glucan and PASC to liberate soluble cello-oligosaccharides, indicating that CenA is the most suitable enzyme for cellulose degradation among those cellulolytic enzymes expressed in
Z
.
palmae
. Furthermore, the
cenA
gene and β-glucosidase gene (
bgl
) from
Ruminococcus albus
were co-expressed in
Z
.
palmae
. Of the total endoglucanase and β-glucosidase activities, 57.1 and 18.1 % were localized in the culture medium of the strain. The genetically engineered strain completely saccharified and fermented 20 g/l barley β-glucan to ethanol within 84 h, producing 79.5 % of the theoretical yield. Thus, the production and secretion of CenA and BGL enabled
Z
.
palmae
to efficiently ferment a water-soluble cellulosic polysaccharide to ethanol.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-013-4874-1</identifier><identifier>PMID: 23604558</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Alcohol ; Alcohol, Denatured ; beta-Glucans - isolation & purification ; beta-Glucans - metabolism ; beta-Glucosidase - genetics ; beta-Glucosidase - metabolism ; Bioenergy and Biofuels ; Biomedical and Life Sciences ; Biotechnology ; Cellulase - genetics ; Cellulase - metabolism ; Cellulomonas ; Cellulomonas - enzymology ; Cellulomonas - genetics ; Cellulomonas fimi ; Cellulose ; Degradation ; Endoglucanase ; Enzymes ; Ethanol ; Ethanol - metabolism ; Ethyl alcohol ; Gene Expression ; Genes ; Genetic engineering ; Genetically modified organisms ; Halomonadaceae - enzymology ; Halomonadaceae - genetics ; Halomonadaceae - metabolism ; Hordeum - chemistry ; Hordeum vulgare ; Hydrolysis ; Life Sciences ; Metabolic Engineering ; Microbial Genetics and Genomics ; Microbiology ; Phosphates ; Phosphoric acid ; Production data ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; Ruminococcus ; Ruminococcus - enzymology ; Ruminococcus - genetics ; Ruminococcus albus ; Strain ; Toy industry</subject><ispartof>Applied microbiology and biotechnology, 2013-06, Vol.97 (11), p.5137-5147</ispartof><rights>Springer-Verlag Berlin Heidelberg 2013</rights><rights>COPYRIGHT 2013 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-f56fdf827cf4efa51770d7b0d451f429f95f1aa649e55c5e09e9a95af2c572653</citedby><cites>FETCH-LOGICAL-c511t-f56fdf827cf4efa51770d7b0d451f429f95f1aa649e55c5e09e9a95af2c572653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00253-013-4874-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00253-013-4874-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23604558$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kojima, Motoki</creatorcontrib><creatorcontrib>Okamoto, Kenji</creatorcontrib><creatorcontrib>Yanase, Hideshi</creatorcontrib><title>Direct ethanol production from cellulosic materials by Zymobacter palmae carrying Cellulomonas endoglucanase and Ruminococcus β-glucosidase genes</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>In order to reduce the cost of bioethanol production from lignocellulosic biomass, we conferred the ability to ferment cellulosic materials directly on
Zymobacter palmae
by co-expressing foreign endoglucanase and β-glucosidase genes.
Z
.
palmae
is a novel ethanol-fermenting bacterium capable of utilizing a broad range of sugar substrates, but not cellulose. Therefore, the six genes encoding the cellulolytic enzymes (CenA, CenB, CenD, CbhA, CbhB, and Cex) from
Cellulomonas fimi
were introduced and expressed in
Z
.
palmae
. Of these cellulolytic enzyme genes cloned, CenA degraded carboxymethylcellulose and phosphoric acid-swollen cellulose (PASC) efficiently. The extracellular CenA catalyzed the hydrolysis of barley β-glucan and PASC to liberate soluble cello-oligosaccharides, indicating that CenA is the most suitable enzyme for cellulose degradation among those cellulolytic enzymes expressed in
Z
.
palmae
. Furthermore, the
cenA
gene and β-glucosidase gene (
bgl
) from
Ruminococcus albus
were co-expressed in
Z
.
palmae
. Of the total endoglucanase and β-glucosidase activities, 57.1 and 18.1 % were localized in the culture medium of the strain. The genetically engineered strain completely saccharified and fermented 20 g/l barley β-glucan to ethanol within 84 h, producing 79.5 % of the theoretical yield. Thus, the production and secretion of CenA and BGL enabled
Z
.
palmae
to efficiently ferment a water-soluble cellulosic polysaccharide to ethanol.</description><subject>Alcohol</subject><subject>Alcohol, Denatured</subject><subject>beta-Glucans - isolation & purification</subject><subject>beta-Glucans - metabolism</subject><subject>beta-Glucosidase - genetics</subject><subject>beta-Glucosidase - metabolism</subject><subject>Bioenergy and Biofuels</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cellulase - genetics</subject><subject>Cellulase - metabolism</subject><subject>Cellulomonas</subject><subject>Cellulomonas - enzymology</subject><subject>Cellulomonas - genetics</subject><subject>Cellulomonas fimi</subject><subject>Cellulose</subject><subject>Degradation</subject><subject>Endoglucanase</subject><subject>Enzymes</subject><subject>Ethanol</subject><subject>Ethanol - metabolism</subject><subject>Ethyl alcohol</subject><subject>Gene Expression</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Genetically modified organisms</subject><subject>Halomonadaceae - enzymology</subject><subject>Halomonadaceae - genetics</subject><subject>Halomonadaceae - metabolism</subject><subject>Hordeum - chemistry</subject><subject>Hordeum vulgare</subject><subject>Hydrolysis</subject><subject>Life Sciences</subject><subject>Metabolic Engineering</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Phosphates</subject><subject>Phosphoric acid</subject><subject>Production data</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - metabolism</subject><subject>Ruminococcus</subject><subject>Ruminococcus - enzymology</subject><subject>Ruminococcus - genetics</subject><subject>Ruminococcus albus</subject><subject>Strain</subject><subject>Toy industry</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks-K1TAUxosoznX0AdxIwI0uOuZv0y6Hq6MDA8KoGzchNz2pGdrkmjTgfQ0fxQeZZzKlo3BBVLIIOef3feEkX1U9JfiMYCxfJYypYDUmrOat5DW5V20IZ7TGDeH3qw0mUtRSdO1J9SilG4wJbZvmYXVCWYO5EO2m-v7aRTAzgvmL9mFE-xj6bGYXPLIxTMjAOOYxJGfQpGeITo8J7Q7o82EKO21KBe31OGlARsd4cH5A21UyBa8TAt-HYcxGlwMg7Xt0nSfngwnG5IRuf9RLt_j3S38AD-lx9cCWW-DJ3X5afbp483H7rr56__Zye35VG0HIXFvR2N62VBrLwWpBpMS93OGeC2I57WwnLNG64R0IYQTgDjrdCW2pEZI2gp1WL1bfMvPXDGlWk0vLvNpDyEmRRpIGS0nbf6NMNF15U0n_B2WYtS3vCvp8RQc9gnLehjlqs-DqnDGJJeacFOrsD1RZPUzOBA_WlfqR4OWRoDAzfJsHnVNSlx-uj1mysiaGlCJYtY9u0vGgCFZLxtSaMVUyppaMqUXz7G7CvJug_634FaoC0BVIpeUHiOom5OjLX_7F9SdbX91a</recordid><startdate>20130601</startdate><enddate>20130601</enddate><creator>Kojima, Motoki</creator><creator>Okamoto, Kenji</creator><creator>Yanase, Hideshi</creator><general>Springer-Verlag</general><general>Springer</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>7X8</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7U5</scope><scope>F28</scope><scope>L7M</scope></search><sort><creationdate>20130601</creationdate><title>Direct ethanol production from cellulosic materials by Zymobacter palmae carrying Cellulomonas endoglucanase and Ruminococcus β-glucosidase genes</title><author>Kojima, Motoki ; Okamoto, Kenji ; Yanase, Hideshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c511t-f56fdf827cf4efa51770d7b0d451f429f95f1aa649e55c5e09e9a95af2c572653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Alcohol</topic><topic>Alcohol, Denatured</topic><topic>beta-Glucans - isolation & purification</topic><topic>beta-Glucans - metabolism</topic><topic>beta-Glucosidase - genetics</topic><topic>beta-Glucosidase - metabolism</topic><topic>Bioenergy and Biofuels</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cellulase - genetics</topic><topic>Cellulase - metabolism</topic><topic>Cellulomonas</topic><topic>Cellulomonas - enzymology</topic><topic>Cellulomonas - genetics</topic><topic>Cellulomonas fimi</topic><topic>Cellulose</topic><topic>Degradation</topic><topic>Endoglucanase</topic><topic>Enzymes</topic><topic>Ethanol</topic><topic>Ethanol - metabolism</topic><topic>Ethyl alcohol</topic><topic>Gene Expression</topic><topic>Genes</topic><topic>Genetic engineering</topic><topic>Genetically modified organisms</topic><topic>Halomonadaceae - enzymology</topic><topic>Halomonadaceae - genetics</topic><topic>Halomonadaceae - metabolism</topic><topic>Hordeum - chemistry</topic><topic>Hordeum vulgare</topic><topic>Hydrolysis</topic><topic>Life Sciences</topic><topic>Metabolic Engineering</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Phosphates</topic><topic>Phosphoric acid</topic><topic>Production data</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - metabolism</topic><topic>Ruminococcus</topic><topic>Ruminococcus - enzymology</topic><topic>Ruminococcus - genetics</topic><topic>Ruminococcus albus</topic><topic>Strain</topic><topic>Toy industry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kojima, Motoki</creatorcontrib><creatorcontrib>Okamoto, Kenji</creatorcontrib><creatorcontrib>Yanase, Hideshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kojima, Motoki</au><au>Okamoto, Kenji</au><au>Yanase, Hideshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct ethanol production from cellulosic materials by Zymobacter palmae carrying Cellulomonas endoglucanase and Ruminococcus β-glucosidase genes</atitle><jtitle>Applied microbiology and biotechnology</jtitle><stitle>Appl Microbiol Biotechnol</stitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2013-06-01</date><risdate>2013</risdate><volume>97</volume><issue>11</issue><spage>5137</spage><epage>5147</epage><pages>5137-5147</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><abstract>In order to reduce the cost of bioethanol production from lignocellulosic biomass, we conferred the ability to ferment cellulosic materials directly on
Zymobacter palmae
by co-expressing foreign endoglucanase and β-glucosidase genes.
Z
.
palmae
is a novel ethanol-fermenting bacterium capable of utilizing a broad range of sugar substrates, but not cellulose. Therefore, the six genes encoding the cellulolytic enzymes (CenA, CenB, CenD, CbhA, CbhB, and Cex) from
Cellulomonas fimi
were introduced and expressed in
Z
.
palmae
. Of these cellulolytic enzyme genes cloned, CenA degraded carboxymethylcellulose and phosphoric acid-swollen cellulose (PASC) efficiently. The extracellular CenA catalyzed the hydrolysis of barley β-glucan and PASC to liberate soluble cello-oligosaccharides, indicating that CenA is the most suitable enzyme for cellulose degradation among those cellulolytic enzymes expressed in
Z
.
palmae
. Furthermore, the
cenA
gene and β-glucosidase gene (
bgl
) from
Ruminococcus albus
were co-expressed in
Z
.
palmae
. Of the total endoglucanase and β-glucosidase activities, 57.1 and 18.1 % were localized in the culture medium of the strain. The genetically engineered strain completely saccharified and fermented 20 g/l barley β-glucan to ethanol within 84 h, producing 79.5 % of the theoretical yield. Thus, the production and secretion of CenA and BGL enabled
Z
.
palmae
to efficiently ferment a water-soluble cellulosic polysaccharide to ethanol.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>23604558</pmid><doi>10.1007/s00253-013-4874-1</doi><tpages>11</tpages></addata></record> |
fulltext | fulltext |
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ispartof | Applied microbiology and biotechnology, 2013-06, Vol.97 (11), p.5137-5147 |
issn | 0175-7598 1432-0614 |
language | eng |
recordid | cdi_proquest_miscellaneous_1671607728 |
source | MEDLINE; SpringerLink Journals |
subjects | Alcohol Alcohol, Denatured beta-Glucans - isolation & purification beta-Glucans - metabolism beta-Glucosidase - genetics beta-Glucosidase - metabolism Bioenergy and Biofuels Biomedical and Life Sciences Biotechnology Cellulase - genetics Cellulase - metabolism Cellulomonas Cellulomonas - enzymology Cellulomonas - genetics Cellulomonas fimi Cellulose Degradation Endoglucanase Enzymes Ethanol Ethanol - metabolism Ethyl alcohol Gene Expression Genes Genetic engineering Genetically modified organisms Halomonadaceae - enzymology Halomonadaceae - genetics Halomonadaceae - metabolism Hordeum - chemistry Hordeum vulgare Hydrolysis Life Sciences Metabolic Engineering Microbial Genetics and Genomics Microbiology Phosphates Phosphoric acid Production data Recombinant Proteins - genetics Recombinant Proteins - metabolism Ruminococcus Ruminococcus - enzymology Ruminococcus - genetics Ruminococcus albus Strain Toy industry |
title | Direct ethanol production from cellulosic materials by Zymobacter palmae carrying Cellulomonas endoglucanase and Ruminococcus β-glucosidase genes |
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