Nutrient limitation in Auxenochlorella protothecoides induces qualitative changes of fatty acid and expression of caleosin as a membrane protein associated with oil bodies
Oil bodies formed in Auxenochlorella protothecoides induced during limited nutrition had a coating of caleosin. The total lipid content obtained from A. protothecoides in unstressed cultures (first week) was ~210 mg/g compared to the 231 mg/g obtained in the third week (nutrient limited) and 290 mg/...
Gespeichert in:
| Veröffentlicht in: | Biotechnology letters 2014, Vol.36 (1), p.175-180 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 180 |
|---|---|
| container_issue | 1 |
| container_start_page | 175 |
| container_title | Biotechnology letters |
| container_volume | 36 |
| creator | Pasaribu, Buntora Lin, I-Ping Chen, Chii-Shiarng Lu, Chi-Yu Jiang, Pei-Luen |
| description | Oil bodies formed in
Auxenochlorella protothecoides
induced during limited nutrition had a coating of caleosin. The total lipid content obtained from
A. protothecoides
in unstressed cultures (first week) was ~210 mg/g compared to the 231 mg/g obtained in the third week (nutrient limited) and 290 mg/g obtained in the fourth week (nutrient limited). The proportion of total saturated fatty acids increased from 28 to 46 %, whereas that of total polyunsaturated fatty acids decreased from 52 to 35 %. The expression levels of the 28 kDa caleosin protein in
A. protothecoides
rose to a maximum up to 4 weeks; immunolocalization studies showed that caleosin was predominantly associated with the membranes of oil bodies. |
| doi_str_mv | 10.1007/s10529-013-1332-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1671602784</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1501372320</sourcerecordid><originalsourceid>FETCH-LOGICAL-c475t-c8e0301716a87214faaa9a34fb0c4aa5989a584bad243882f51917afdd61b1923</originalsourceid><addsrcrecordid>eNqFks9u1DAQxi0EosvCA3BBlrhwCXgcJ46PVcU_qYILnKOJPWFdJfHWdqB9Jl4S725BCAn14pE8v_lm7PkYew7iNQih3yQQjTSVgLqCupYVPGAbaHRdtVq3D9lGgIKqUUaesScpXQkhjBb6MTuTSugOpN6wn5_WHD0tmU9-9hmzDwv3Cz9fb2gJdjeFSNOEfB9DDnlHNnhHqRButSVerzgdq74TtztcvpW7MPIRc77laL3juDhON_tIKR2kS9LiRCGVHpg48pnmIeJCxw50vE3Beszk-A-fdzz4iQ_BeUpP2aMRp0TP7uKWfX339svFh-ry8_uPF-eXlVW6yZXtSNQCNLTYaQlqRESDtRoHYRViYzqDTacGdFLVXSfHBgxoHJ1rYQAj6y17ddItI12vlHI_-2QP37BQWFMPbdEWUnfqfrQpy9GyluJ-tOypBTDtQfXlP-hVWONS3lworUTBWlMoOFE2hpQijf0--hnjbQ-iP_ijP_mjLyP0B3-UY8te3Cmvw0zuT8VvQxRAnoBUUmWd8a_W_1X9BTMux-s</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1474061169</pqid></control><display><type>article</type><title>Nutrient limitation in Auxenochlorella protothecoides induces qualitative changes of fatty acid and expression of caleosin as a membrane protein associated with oil bodies</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Pasaribu, Buntora ; Lin, I-Ping ; Chen, Chii-Shiarng ; Lu, Chi-Yu ; Jiang, Pei-Luen</creator><creatorcontrib>Pasaribu, Buntora ; Lin, I-Ping ; Chen, Chii-Shiarng ; Lu, Chi-Yu ; Jiang, Pei-Luen</creatorcontrib><description>Oil bodies formed in
Auxenochlorella protothecoides
induced during limited nutrition had a coating of caleosin. The total lipid content obtained from
A. protothecoides
in unstressed cultures (first week) was ~210 mg/g compared to the 231 mg/g obtained in the third week (nutrient limited) and 290 mg/g obtained in the fourth week (nutrient limited). The proportion of total saturated fatty acids increased from 28 to 46 %, whereas that of total polyunsaturated fatty acids decreased from 52 to 35 %. The expression levels of the 28 kDa caleosin protein in
A. protothecoides
rose to a maximum up to 4 weeks; immunolocalization studies showed that caleosin was predominantly associated with the membranes of oil bodies.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/s10529-013-1332-1</identifier><identifier>PMID: 24078127</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Algae ; Applied Microbiology ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Calcium-Binding Proteins - analysis ; Calcium-Binding Proteins - metabolism ; Chlorophyta - chemistry ; Chlorophyta - cytology ; Chlorophyta - physiology ; Culture ; Fatty acids ; Fatty Acids - analysis ; Fatty Acids - chemistry ; Fatty Acids - metabolism ; Hydrophobic and Hydrophilic Interactions ; Life Sciences ; Lipids ; Membrane Proteins - analysis ; Membrane Proteins - metabolism ; Membranes ; Microbiology ; Nitrogen - metabolism ; Nutrients ; Nutrition ; Organelles - metabolism ; Original Research Paper ; Plant Proteins - analysis ; Plant Proteins - metabolism ; Polyunsaturated fatty acids ; Proteins ; Studies</subject><ispartof>Biotechnology letters, 2014, Vol.36 (1), p.175-180</ispartof><rights>Springer Science+Business Media Dordrecht 2013</rights><rights>Springer Science+Business Media Dordrecht 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-c8e0301716a87214faaa9a34fb0c4aa5989a584bad243882f51917afdd61b1923</citedby><cites>FETCH-LOGICAL-c475t-c8e0301716a87214faaa9a34fb0c4aa5989a584bad243882f51917afdd61b1923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10529-013-1332-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10529-013-1332-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24078127$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pasaribu, Buntora</creatorcontrib><creatorcontrib>Lin, I-Ping</creatorcontrib><creatorcontrib>Chen, Chii-Shiarng</creatorcontrib><creatorcontrib>Lu, Chi-Yu</creatorcontrib><creatorcontrib>Jiang, Pei-Luen</creatorcontrib><title>Nutrient limitation in Auxenochlorella protothecoides induces qualitative changes of fatty acid and expression of caleosin as a membrane protein associated with oil bodies</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><addtitle>Biotechnol Lett</addtitle><description>Oil bodies formed in
Auxenochlorella protothecoides
induced during limited nutrition had a coating of caleosin. The total lipid content obtained from
A. protothecoides
in unstressed cultures (first week) was ~210 mg/g compared to the 231 mg/g obtained in the third week (nutrient limited) and 290 mg/g obtained in the fourth week (nutrient limited). The proportion of total saturated fatty acids increased from 28 to 46 %, whereas that of total polyunsaturated fatty acids decreased from 52 to 35 %. The expression levels of the 28 kDa caleosin protein in
A. protothecoides
rose to a maximum up to 4 weeks; immunolocalization studies showed that caleosin was predominantly associated with the membranes of oil bodies.</description><subject>Algae</subject><subject>Applied Microbiology</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Calcium-Binding Proteins - analysis</subject><subject>Calcium-Binding Proteins - metabolism</subject><subject>Chlorophyta - chemistry</subject><subject>Chlorophyta - cytology</subject><subject>Chlorophyta - physiology</subject><subject>Culture</subject><subject>Fatty acids</subject><subject>Fatty Acids - analysis</subject><subject>Fatty Acids - chemistry</subject><subject>Fatty Acids - metabolism</subject><subject>Hydrophobic and Hydrophilic Interactions</subject><subject>Life Sciences</subject><subject>Lipids</subject><subject>Membrane Proteins - analysis</subject><subject>Membrane Proteins - metabolism</subject><subject>Membranes</subject><subject>Microbiology</subject><subject>Nitrogen - metabolism</subject><subject>Nutrients</subject><subject>Nutrition</subject><subject>Organelles - metabolism</subject><subject>Original Research Paper</subject><subject>Plant Proteins - analysis</subject><subject>Plant Proteins - metabolism</subject><subject>Polyunsaturated fatty acids</subject><subject>Proteins</subject><subject>Studies</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFks9u1DAQxi0EosvCA3BBlrhwCXgcJ46PVcU_qYILnKOJPWFdJfHWdqB9Jl4S725BCAn14pE8v_lm7PkYew7iNQih3yQQjTSVgLqCupYVPGAbaHRdtVq3D9lGgIKqUUaesScpXQkhjBb6MTuTSugOpN6wn5_WHD0tmU9-9hmzDwv3Cz9fb2gJdjeFSNOEfB9DDnlHNnhHqRButSVerzgdq74TtztcvpW7MPIRc77laL3juDhON_tIKR2kS9LiRCGVHpg48pnmIeJCxw50vE3Beszk-A-fdzz4iQ_BeUpP2aMRp0TP7uKWfX339svFh-ry8_uPF-eXlVW6yZXtSNQCNLTYaQlqRESDtRoHYRViYzqDTacGdFLVXSfHBgxoHJ1rYQAj6y17ddItI12vlHI_-2QP37BQWFMPbdEWUnfqfrQpy9GyluJ-tOypBTDtQfXlP-hVWONS3lworUTBWlMoOFE2hpQijf0--hnjbQ-iP_ijP_mjLyP0B3-UY8te3Cmvw0zuT8VvQxRAnoBUUmWd8a_W_1X9BTMux-s</recordid><startdate>2014</startdate><enddate>2014</enddate><creator>Pasaribu, Buntora</creator><creator>Lin, I-Ping</creator><creator>Chen, Chii-Shiarng</creator><creator>Lu, Chi-Yu</creator><creator>Jiang, Pei-Luen</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TB</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7QO</scope><scope>7ST</scope><scope>SOI</scope><scope>7X8</scope><scope>7SU</scope></search><sort><creationdate>2014</creationdate><title>Nutrient limitation in Auxenochlorella protothecoides induces qualitative changes of fatty acid and expression of caleosin as a membrane protein associated with oil bodies</title><author>Pasaribu, Buntora ; Lin, I-Ping ; Chen, Chii-Shiarng ; Lu, Chi-Yu ; Jiang, Pei-Luen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-c8e0301716a87214faaa9a34fb0c4aa5989a584bad243882f51917afdd61b1923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Algae</topic><topic>Applied Microbiology</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Calcium-Binding Proteins - analysis</topic><topic>Calcium-Binding Proteins - metabolism</topic><topic>Chlorophyta - chemistry</topic><topic>Chlorophyta - cytology</topic><topic>Chlorophyta - physiology</topic><topic>Culture</topic><topic>Fatty acids</topic><topic>Fatty Acids - analysis</topic><topic>Fatty Acids - chemistry</topic><topic>Fatty Acids - metabolism</topic><topic>Hydrophobic and Hydrophilic Interactions</topic><topic>Life Sciences</topic><topic>Lipids</topic><topic>Membrane Proteins - analysis</topic><topic>Membrane Proteins - metabolism</topic><topic>Membranes</topic><topic>Microbiology</topic><topic>Nitrogen - metabolism</topic><topic>Nutrients</topic><topic>Nutrition</topic><topic>Organelles - metabolism</topic><topic>Original Research Paper</topic><topic>Plant Proteins - analysis</topic><topic>Plant Proteins - metabolism</topic><topic>Polyunsaturated fatty acids</topic><topic>Proteins</topic><topic>Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pasaribu, Buntora</creatorcontrib><creatorcontrib>Lin, I-Ping</creatorcontrib><creatorcontrib>Chen, Chii-Shiarng</creatorcontrib><creatorcontrib>Lu, Chi-Yu</creatorcontrib><creatorcontrib>Jiang, Pei-Luen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Environmental Engineering Abstracts</collection><jtitle>Biotechnology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pasaribu, Buntora</au><au>Lin, I-Ping</au><au>Chen, Chii-Shiarng</au><au>Lu, Chi-Yu</au><au>Jiang, Pei-Luen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nutrient limitation in Auxenochlorella protothecoides induces qualitative changes of fatty acid and expression of caleosin as a membrane protein associated with oil bodies</atitle><jtitle>Biotechnology letters</jtitle><stitle>Biotechnol Lett</stitle><addtitle>Biotechnol Lett</addtitle><date>2014</date><risdate>2014</risdate><volume>36</volume><issue>1</issue><spage>175</spage><epage>180</epage><pages>175-180</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><abstract>Oil bodies formed in
Auxenochlorella protothecoides
induced during limited nutrition had a coating of caleosin. The total lipid content obtained from
A. protothecoides
in unstressed cultures (first week) was ~210 mg/g compared to the 231 mg/g obtained in the third week (nutrient limited) and 290 mg/g obtained in the fourth week (nutrient limited). The proportion of total saturated fatty acids increased from 28 to 46 %, whereas that of total polyunsaturated fatty acids decreased from 52 to 35 %. The expression levels of the 28 kDa caleosin protein in
A. protothecoides
rose to a maximum up to 4 weeks; immunolocalization studies showed that caleosin was predominantly associated with the membranes of oil bodies.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>24078127</pmid><doi>10.1007/s10529-013-1332-1</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0141-5492 |
| ispartof | Biotechnology letters, 2014, Vol.36 (1), p.175-180 |
| issn | 0141-5492 1573-6776 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1671602784 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Algae Applied Microbiology Biochemistry Biomedical and Life Sciences Biotechnology Calcium-Binding Proteins - analysis Calcium-Binding Proteins - metabolism Chlorophyta - chemistry Chlorophyta - cytology Chlorophyta - physiology Culture Fatty acids Fatty Acids - analysis Fatty Acids - chemistry Fatty Acids - metabolism Hydrophobic and Hydrophilic Interactions Life Sciences Lipids Membrane Proteins - analysis Membrane Proteins - metabolism Membranes Microbiology Nitrogen - metabolism Nutrients Nutrition Organelles - metabolism Original Research Paper Plant Proteins - analysis Plant Proteins - metabolism Polyunsaturated fatty acids Proteins Studies |
| title | Nutrient limitation in Auxenochlorella protothecoides induces qualitative changes of fatty acid and expression of caleosin as a membrane protein associated with oil bodies |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T14%3A40%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Nutrient%20limitation%20in%20Auxenochlorella%20protothecoides%20induces%20qualitative%20changes%20of%20fatty%20acid%20and%20expression%20of%20caleosin%20as%20a%20membrane%20protein%20associated%20with%20oil%20bodies&rft.jtitle=Biotechnology%20letters&rft.au=Pasaribu,%20Buntora&rft.date=2014&rft.volume=36&rft.issue=1&rft.spage=175&rft.epage=180&rft.pages=175-180&rft.issn=0141-5492&rft.eissn=1573-6776&rft_id=info:doi/10.1007/s10529-013-1332-1&rft_dat=%3Cproquest_cross%3E1501372320%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1474061169&rft_id=info:pmid/24078127&rfr_iscdi=true |