Isolation and identification of an iopromide-degrading strain and its application in an A2/O system
► Isolate and identify an iopromide-degrading strain I-24. ► Evaluate the effects of various factors on IOPr degradation by strain I-24. ► Examine the enzymatic activity of I-24 supplied with different carbon sources. ► Apply strain I-24 to a lab-scale waste water treatment process. ► Analyse the 16...
Gespeichert in:
| Veröffentlicht in: | Bioresource technology 2013-04, Vol.134, p.36-42 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 42 |
|---|---|
| container_issue | |
| container_start_page | 36 |
| container_title | Bioresource technology |
| container_volume | 134 |
| creator | Liu, Yanan Hu, Jinlong Xu, Bingjie He, Jun Gao, Pin Liu, Kaiying Xue, Gang Ognier, Stéphanie |
| description | ► Isolate and identify an iopromide-degrading strain I-24. ► Evaluate the effects of various factors on IOPr degradation by strain I-24. ► Examine the enzymatic activity of I-24 supplied with different carbon sources. ► Apply strain I-24 to a lab-scale waste water treatment process. ► Analyse the 16S rDNA fragments obtained from the reactor by DGGE and UPGMA cluster.
An iopromide (IOPr)-degrading bacterium was isolated from activated sludge of a wastewater treatment plant in Shanghai. Based on its morphology, physiological-biochemical characteristics and a phylogenetic analysis of its 16S rRNA sequence, the bacterium was identified and named as Pseudomonas sp. I-24. The optimum condition for degrading IOPr was at 30°C and pH 7.0. After 5days, strain I-24 could degrade 30mg/L IOPr by 99% in a basal salts medium with a 5% (V/V) inoculum and 200mg/L starch as the primary substrate. When applied to an Anaerobic-Anoxic/Aerobic (A2/O) process, with the coexistence of other bacteria, the strain I-24 got lower (61.3%) IOPr removal, but in two A2/O systems (with and without I-24 inoculation), the CODcr removal were both approximately 95%. The trial dosed with strain I-24 showed better IOPr removal than the un-dosed one. I-24 sustained its abundance in the A2/O system during the experiment. |
| doi_str_mv | 10.1016/j.biortech.2013.02.043 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1671576407</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0960852413002666</els_id><sourcerecordid>1500771385</sourcerecordid><originalsourceid>FETCH-LOGICAL-c394t-b8fb1fb6846f96e2f9686ef88077dcb0031e55b1ff34c14ebcb3fb4c8609869d3</originalsourceid><addsrcrecordid>eNqFkUtrGzEUhUVoadykfyHMptDNTPSWZtcQ2iYQyKZZCz2uEpl5uNK4kH8fubbTpTcSHH3n6nAPQlcEdwQTeb3uXJrzAv6lo5iwDtMOc3aGVkQr1tJeyQ9ohXuJWy0oP0efS1ljjBlR9BM6p0xgLIRaIX9f5sEuaZ4aO4UmBZiWFJPfS3OsapPmTZ7H-tQGeM42pOm5KUu26eBZSmM3m-Fo-ic3N_T6sSmvZYHxEn2Mdijw5XBfoKefP37f3rUPj7_ub28eWs96vrROR0eik5rL2Eug9dASotZYqeDdLjwIUZHIuCccnHcsOu61xL2WfWAX6Nt-bo37ZwtlMWMqHobBTjBviyFSEaEkx-o0WvejFGFanEYZFYxRzmhF5R71eS4lQzSbnEabXw3BZlebWZtjbWZXm8HU1Nqq8erwx9aNEN5tx54q8PUA2OLtELOdfCr_OUU1Y2o36Pueg7rnvwmyKT7B5CGkDH4xYU6nsrwBFBm4xg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1325332432</pqid></control><display><type>article</type><title>Isolation and identification of an iopromide-degrading strain and its application in an A2/O system</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Liu, Yanan ; Hu, Jinlong ; Xu, Bingjie ; He, Jun ; Gao, Pin ; Liu, Kaiying ; Xue, Gang ; Ognier, Stéphanie</creator><creatorcontrib>Liu, Yanan ; Hu, Jinlong ; Xu, Bingjie ; He, Jun ; Gao, Pin ; Liu, Kaiying ; Xue, Gang ; Ognier, Stéphanie</creatorcontrib><description>► Isolate and identify an iopromide-degrading strain I-24. ► Evaluate the effects of various factors on IOPr degradation by strain I-24. ► Examine the enzymatic activity of I-24 supplied with different carbon sources. ► Apply strain I-24 to a lab-scale waste water treatment process. ► Analyse the 16S rDNA fragments obtained from the reactor by DGGE and UPGMA cluster.
An iopromide (IOPr)-degrading bacterium was isolated from activated sludge of a wastewater treatment plant in Shanghai. Based on its morphology, physiological-biochemical characteristics and a phylogenetic analysis of its 16S rRNA sequence, the bacterium was identified and named as Pseudomonas sp. I-24. The optimum condition for degrading IOPr was at 30°C and pH 7.0. After 5days, strain I-24 could degrade 30mg/L IOPr by 99% in a basal salts medium with a 5% (V/V) inoculum and 200mg/L starch as the primary substrate. When applied to an Anaerobic-Anoxic/Aerobic (A2/O) process, with the coexistence of other bacteria, the strain I-24 got lower (61.3%) IOPr removal, but in two A2/O systems (with and without I-24 inoculation), the CODcr removal were both approximately 95%. The trial dosed with strain I-24 showed better IOPr removal than the un-dosed one. I-24 sustained its abundance in the A2/O system during the experiment.</description><identifier>ISSN: 0960-8524</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2013.02.043</identifier><identifier>PMID: 23500557</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Aerobiosis ; Anaerobic processes ; Anaerobiosis ; Bacteria ; Biodegradation, Environmental ; Biological and medical sciences ; Biological Oxygen Demand Analysis ; Bioreactors - microbiology ; Co-metabolism ; Colony Count, Microbial ; Degradation ; Denaturing Gradient Gel Electrophoresis ; Fundamental and applied biological sciences. Psychology ; Inoculum ; Iohexol - analogs & derivatives ; Iohexol - metabolism ; Iopromide ; Isolation and identification ; Kinetics ; Molecular Sequence Data ; Optimization ; Phylogeny ; Pseudomonas ; Pseudomonas - enzymology ; Pseudomonas - growth & development ; Pseudomonas - isolation & purification ; Pseudomonas - ultrastructure ; Pseudomonas sp ; RNA, Ribosomal, 16S - genetics ; Sewage - microbiology ; Starches ; Strain ; Strain I-24 ; Waste Water - microbiology ; Water Purification</subject><ispartof>Bioresource technology, 2013-04, Vol.134, p.36-42</ispartof><rights>2013 Elsevier Ltd</rights><rights>2014 INIST-CNRS</rights><rights>Copyright © 2013 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c394t-b8fb1fb6846f96e2f9686ef88077dcb0031e55b1ff34c14ebcb3fb4c8609869d3</citedby><cites>FETCH-LOGICAL-c394t-b8fb1fb6846f96e2f9686ef88077dcb0031e55b1ff34c14ebcb3fb4c8609869d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biortech.2013.02.043$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27283373$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23500557$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Yanan</creatorcontrib><creatorcontrib>Hu, Jinlong</creatorcontrib><creatorcontrib>Xu, Bingjie</creatorcontrib><creatorcontrib>He, Jun</creatorcontrib><creatorcontrib>Gao, Pin</creatorcontrib><creatorcontrib>Liu, Kaiying</creatorcontrib><creatorcontrib>Xue, Gang</creatorcontrib><creatorcontrib>Ognier, Stéphanie</creatorcontrib><title>Isolation and identification of an iopromide-degrading strain and its application in an A2/O system</title><title>Bioresource technology</title><addtitle>Bioresour Technol</addtitle><description>► Isolate and identify an iopromide-degrading strain I-24. ► Evaluate the effects of various factors on IOPr degradation by strain I-24. ► Examine the enzymatic activity of I-24 supplied with different carbon sources. ► Apply strain I-24 to a lab-scale waste water treatment process. ► Analyse the 16S rDNA fragments obtained from the reactor by DGGE and UPGMA cluster.
An iopromide (IOPr)-degrading bacterium was isolated from activated sludge of a wastewater treatment plant in Shanghai. Based on its morphology, physiological-biochemical characteristics and a phylogenetic analysis of its 16S rRNA sequence, the bacterium was identified and named as Pseudomonas sp. I-24. The optimum condition for degrading IOPr was at 30°C and pH 7.0. After 5days, strain I-24 could degrade 30mg/L IOPr by 99% in a basal salts medium with a 5% (V/V) inoculum and 200mg/L starch as the primary substrate. When applied to an Anaerobic-Anoxic/Aerobic (A2/O) process, with the coexistence of other bacteria, the strain I-24 got lower (61.3%) IOPr removal, but in two A2/O systems (with and without I-24 inoculation), the CODcr removal were both approximately 95%. The trial dosed with strain I-24 showed better IOPr removal than the un-dosed one. I-24 sustained its abundance in the A2/O system during the experiment.</description><subject>Aerobiosis</subject><subject>Anaerobic processes</subject><subject>Anaerobiosis</subject><subject>Bacteria</subject><subject>Biodegradation, Environmental</subject><subject>Biological and medical sciences</subject><subject>Biological Oxygen Demand Analysis</subject><subject>Bioreactors - microbiology</subject><subject>Co-metabolism</subject><subject>Colony Count, Microbial</subject><subject>Degradation</subject><subject>Denaturing Gradient Gel Electrophoresis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Inoculum</subject><subject>Iohexol - analogs & derivatives</subject><subject>Iohexol - metabolism</subject><subject>Iopromide</subject><subject>Isolation and identification</subject><subject>Kinetics</subject><subject>Molecular Sequence Data</subject><subject>Optimization</subject><subject>Phylogeny</subject><subject>Pseudomonas</subject><subject>Pseudomonas - enzymology</subject><subject>Pseudomonas - growth & development</subject><subject>Pseudomonas - isolation & purification</subject><subject>Pseudomonas - ultrastructure</subject><subject>Pseudomonas sp</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sewage - microbiology</subject><subject>Starches</subject><subject>Strain</subject><subject>Strain I-24</subject><subject>Waste Water - microbiology</subject><subject>Water Purification</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtrGzEUhUVoadykfyHMptDNTPSWZtcQ2iYQyKZZCz2uEpl5uNK4kH8fubbTpTcSHH3n6nAPQlcEdwQTeb3uXJrzAv6lo5iwDtMOc3aGVkQr1tJeyQ9ohXuJWy0oP0efS1ljjBlR9BM6p0xgLIRaIX9f5sEuaZ4aO4UmBZiWFJPfS3OsapPmTZ7H-tQGeM42pOm5KUu26eBZSmM3m-Fo-ic3N_T6sSmvZYHxEn2Mdijw5XBfoKefP37f3rUPj7_ub28eWs96vrROR0eik5rL2Eug9dASotZYqeDdLjwIUZHIuCccnHcsOu61xL2WfWAX6Nt-bo37ZwtlMWMqHobBTjBviyFSEaEkx-o0WvejFGFanEYZFYxRzmhF5R71eS4lQzSbnEabXw3BZlebWZtjbWZXm8HU1Nqq8erwx9aNEN5tx54q8PUA2OLtELOdfCr_OUU1Y2o36Pueg7rnvwmyKT7B5CGkDH4xYU6nsrwBFBm4xg</recordid><startdate>20130401</startdate><enddate>20130401</enddate><creator>Liu, Yanan</creator><creator>Hu, Jinlong</creator><creator>Xu, Bingjie</creator><creator>He, Jun</creator><creator>Gao, Pin</creator><creator>Liu, Kaiying</creator><creator>Xue, Gang</creator><creator>Ognier, Stéphanie</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QH</scope><scope>7QO</scope><scope>7UA</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H97</scope><scope>L.G</scope><scope>P64</scope><scope>7SU</scope><scope>7TB</scope><scope>KR7</scope></search><sort><creationdate>20130401</creationdate><title>Isolation and identification of an iopromide-degrading strain and its application in an A2/O system</title><author>Liu, Yanan ; Hu, Jinlong ; Xu, Bingjie ; He, Jun ; Gao, Pin ; Liu, Kaiying ; Xue, Gang ; Ognier, Stéphanie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c394t-b8fb1fb6846f96e2f9686ef88077dcb0031e55b1ff34c14ebcb3fb4c8609869d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Aerobiosis</topic><topic>Anaerobic processes</topic><topic>Anaerobiosis</topic><topic>Bacteria</topic><topic>Biodegradation, Environmental</topic><topic>Biological and medical sciences</topic><topic>Biological Oxygen Demand Analysis</topic><topic>Bioreactors - microbiology</topic><topic>Co-metabolism</topic><topic>Colony Count, Microbial</topic><topic>Degradation</topic><topic>Denaturing Gradient Gel Electrophoresis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Inoculum</topic><topic>Iohexol - analogs & derivatives</topic><topic>Iohexol - metabolism</topic><topic>Iopromide</topic><topic>Isolation and identification</topic><topic>Kinetics</topic><topic>Molecular Sequence Data</topic><topic>Optimization</topic><topic>Phylogeny</topic><topic>Pseudomonas</topic><topic>Pseudomonas - enzymology</topic><topic>Pseudomonas - growth & development</topic><topic>Pseudomonas - isolation & purification</topic><topic>Pseudomonas - ultrastructure</topic><topic>Pseudomonas sp</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sewage - microbiology</topic><topic>Starches</topic><topic>Strain</topic><topic>Strain I-24</topic><topic>Waste Water - microbiology</topic><topic>Water Purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Yanan</creatorcontrib><creatorcontrib>Hu, Jinlong</creatorcontrib><creatorcontrib>Xu, Bingjie</creatorcontrib><creatorcontrib>He, Jun</creatorcontrib><creatorcontrib>Gao, Pin</creatorcontrib><creatorcontrib>Liu, Kaiying</creatorcontrib><creatorcontrib>Xue, Gang</creatorcontrib><creatorcontrib>Ognier, Stéphanie</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Aqualine</collection><collection>Biotechnology Research Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Engineering Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Civil Engineering Abstracts</collection><jtitle>Bioresource technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Yanan</au><au>Hu, Jinlong</au><au>Xu, Bingjie</au><au>He, Jun</au><au>Gao, Pin</au><au>Liu, Kaiying</au><au>Xue, Gang</au><au>Ognier, Stéphanie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and identification of an iopromide-degrading strain and its application in an A2/O system</atitle><jtitle>Bioresource technology</jtitle><addtitle>Bioresour Technol</addtitle><date>2013-04-01</date><risdate>2013</risdate><volume>134</volume><spage>36</spage><epage>42</epage><pages>36-42</pages><issn>0960-8524</issn><eissn>1873-2976</eissn><abstract>► Isolate and identify an iopromide-degrading strain I-24. ► Evaluate the effects of various factors on IOPr degradation by strain I-24. ► Examine the enzymatic activity of I-24 supplied with different carbon sources. ► Apply strain I-24 to a lab-scale waste water treatment process. ► Analyse the 16S rDNA fragments obtained from the reactor by DGGE and UPGMA cluster.
An iopromide (IOPr)-degrading bacterium was isolated from activated sludge of a wastewater treatment plant in Shanghai. Based on its morphology, physiological-biochemical characteristics and a phylogenetic analysis of its 16S rRNA sequence, the bacterium was identified and named as Pseudomonas sp. I-24. The optimum condition for degrading IOPr was at 30°C and pH 7.0. After 5days, strain I-24 could degrade 30mg/L IOPr by 99% in a basal salts medium with a 5% (V/V) inoculum and 200mg/L starch as the primary substrate. When applied to an Anaerobic-Anoxic/Aerobic (A2/O) process, with the coexistence of other bacteria, the strain I-24 got lower (61.3%) IOPr removal, but in two A2/O systems (with and without I-24 inoculation), the CODcr removal were both approximately 95%. The trial dosed with strain I-24 showed better IOPr removal than the un-dosed one. I-24 sustained its abundance in the A2/O system during the experiment.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>23500557</pmid><doi>10.1016/j.biortech.2013.02.043</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0960-8524 |
| ispartof | Bioresource technology, 2013-04, Vol.134, p.36-42 |
| issn | 0960-8524 1873-2976 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1671576407 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Aerobiosis Anaerobic processes Anaerobiosis Bacteria Biodegradation, Environmental Biological and medical sciences Biological Oxygen Demand Analysis Bioreactors - microbiology Co-metabolism Colony Count, Microbial Degradation Denaturing Gradient Gel Electrophoresis Fundamental and applied biological sciences. Psychology Inoculum Iohexol - analogs & derivatives Iohexol - metabolism Iopromide Isolation and identification Kinetics Molecular Sequence Data Optimization Phylogeny Pseudomonas Pseudomonas - enzymology Pseudomonas - growth & development Pseudomonas - isolation & purification Pseudomonas - ultrastructure Pseudomonas sp RNA, Ribosomal, 16S - genetics Sewage - microbiology Starches Strain Strain I-24 Waste Water - microbiology Water Purification |
| title | Isolation and identification of an iopromide-degrading strain and its application in an A2/O system |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T20%3A47%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Isolation%20and%20identification%20of%20an%20iopromide-degrading%20strain%20and%20its%20application%20in%20an%20A2/O%20system&rft.jtitle=Bioresource%20technology&rft.au=Liu,%20Yanan&rft.date=2013-04-01&rft.volume=134&rft.spage=36&rft.epage=42&rft.pages=36-42&rft.issn=0960-8524&rft.eissn=1873-2976&rft_id=info:doi/10.1016/j.biortech.2013.02.043&rft_dat=%3Cproquest_cross%3E1500771385%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1325332432&rft_id=info:pmid/23500557&rft_els_id=S0960852413002666&rfr_iscdi=true |