Separation of sperm from mixed samples using ultra-high-speed cell sorter followed by DNA typing
DNA typing of samples obtained from crime scenes can be used to identify suspects. However, samples obtained from sex crimes are often mixtures, and it is difficult to separate these for DNA typing. We extracted sperm from mixed samples using an ultra-high-speed cell sorter and examined whether DNA...
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| Veröffentlicht in: | Japanese Journal of Forensic Science and Technology 2014, Vol.19(1), pp.45-55 |
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| container_title | Japanese Journal of Forensic Science and Technology |
| container_volume | 19 |
| creator | Nakamura, Arata Shinozuka, Tatsuo Sonoda, Akira Kurosaki, Kunihiko Saito, Kazuyuki Takada, Aya |
| description | DNA typing of samples obtained from crime scenes can be used to identify suspects. However, samples obtained from sex crimes are often mixtures, and it is difficult to separate these for DNA typing. We extracted sperm from mixed samples using an ultra-high-speed cell sorter and examined whether DNA typing was possible. Propidium iodide was able to react with semen, allowing sperm nuclei to be sorted with the cell sorter. DNA typing was performed using the sorted sperm. The utility of anti-sperm antibody for cell sorting was compared with that of anti-sperm surface protein SP17, and anti-sperm antibody was found to be more suitable. Thus, semen reacted with anti-sperm antibody and with propidium iodide, thereby allowing sperm with nuclei to be collected. DNA typing could then be performed using the sorted sperm. |
| doi_str_mv | 10.3408/jafst.19.45 |
| format | Article |
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However, samples obtained from sex crimes are often mixtures, and it is difficult to separate these for DNA typing. We extracted sperm from mixed samples using an ultra-high-speed cell sorter and examined whether DNA typing was possible. Propidium iodide was able to react with semen, allowing sperm nuclei to be sorted with the cell sorter. DNA typing was performed using the sorted sperm. The utility of anti-sperm antibody for cell sorting was compared with that of anti-sperm surface protein SP17, and anti-sperm antibody was found to be more suitable. Thus, semen reacted with anti-sperm antibody and with propidium iodide, thereby allowing sperm with nuclei to be collected. 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J. Forensic Sci. Technol.</addtitle><description>DNA typing of samples obtained from crime scenes can be used to identify suspects. However, samples obtained from sex crimes are often mixtures, and it is difficult to separate these for DNA typing. We extracted sperm from mixed samples using an ultra-high-speed cell sorter and examined whether DNA typing was possible. Propidium iodide was able to react with semen, allowing sperm nuclei to be sorted with the cell sorter. DNA typing was performed using the sorted sperm. The utility of anti-sperm antibody for cell sorting was compared with that of anti-sperm surface protein SP17, and anti-sperm antibody was found to be more suitable. Thus, semen reacted with anti-sperm antibody and with propidium iodide, thereby allowing sperm with nuclei to be collected. DNA typing could then be performed using the sorted sperm.</description><subject>Anti-sperm antibody</subject><subject>Antibodies</subject><subject>Cell sorter</subject><subject>Crime</subject><subject>Deoxyribonucleic acid</subject><subject>Forensic science</subject><subject>Iodides</subject><subject>Mixed sample</subject><subject>Nuclei</subject><subject>Propidium iodide</subject><subject>Semen</subject><subject>Sperm</subject><subject>Utilities</subject><issn>1880-1323</issn><issn>1881-4689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqF0M1OwzAMAOAKgcQ0duIFckRCHXGTtOkFaRq_EoIDcA5pmmyd2qYkqWBvT7eiceRky_5sWY6ic8BzQjG_2kjjwxzyOWVH0QQ4h5imPD_e5zgGkpDTaOZ9VeAEY0g44En08ao76WSobIusQb7TrkHG2QY11bcukZdNV2uPel-1K9TXwcl4Xa3W8SCHttJ1jbx1QTtkbF3br6FYbNHN8wKFbTfMnEUnRtZez37jNHq_u31bPsRPL_ePy8VTrCjBLE6znDDCeJFkOFE0MVxlFGNJlSK4ZBJyWlJVFJImGmd5KUvQKtWQFjSlmBkyjS7GvZ2zn732QTSV350nW217LyDNgLEMAP6njFAOQGk20MuRKme9d9qIzlWNdFsBWOy-LvZfF5ALygZ9PeqND3KlD1a6UKla_1kYBw4NtZZO6Jb8ALXCjJs</recordid><startdate>201401</startdate><enddate>201401</enddate><creator>Nakamura, Arata</creator><creator>Shinozuka, Tatsuo</creator><creator>Sonoda, Akira</creator><creator>Kurosaki, Kunihiko</creator><creator>Saito, Kazuyuki</creator><creator>Takada, Aya</creator><general>Japanese Association of Forensic Science and Technology</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7TB</scope><scope>8FD</scope><scope>FR3</scope><scope>KR7</scope></search><sort><creationdate>201401</creationdate><title>Separation of sperm from mixed samples using ultra-high-speed cell sorter followed by DNA typing</title><author>Nakamura, Arata ; Shinozuka, Tatsuo ; Sonoda, Akira ; Kurosaki, Kunihiko ; Saito, Kazuyuki ; Takada, Aya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4305-67935358b2702c42f8c7400a4cc30d5a194d4cbba42e079dad1ec6e16b46405f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Anti-sperm antibody</topic><topic>Antibodies</topic><topic>Cell sorter</topic><topic>Crime</topic><topic>Deoxyribonucleic acid</topic><topic>Forensic science</topic><topic>Iodides</topic><topic>Mixed sample</topic><topic>Nuclei</topic><topic>Propidium iodide</topic><topic>Semen</topic><topic>Sperm</topic><topic>Utilities</topic><toplevel>online_resources</toplevel><creatorcontrib>Nakamura, Arata</creatorcontrib><creatorcontrib>Shinozuka, Tatsuo</creatorcontrib><creatorcontrib>Sonoda, Akira</creatorcontrib><creatorcontrib>Kurosaki, Kunihiko</creatorcontrib><creatorcontrib>Saito, Kazuyuki</creatorcontrib><creatorcontrib>Takada, Aya</creatorcontrib><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Civil Engineering Abstracts</collection><jtitle>Japanese Journal of Forensic Science and Technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakamura, Arata</au><au>Shinozuka, Tatsuo</au><au>Sonoda, Akira</au><au>Kurosaki, Kunihiko</au><au>Saito, Kazuyuki</au><au>Takada, Aya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Separation of sperm from mixed samples using ultra-high-speed cell sorter followed by DNA typing</atitle><jtitle>Japanese Journal of Forensic Science and Technology</jtitle><addtitle>Jpn. J. Forensic Sci. Technol.</addtitle><date>2014-01</date><risdate>2014</risdate><volume>19</volume><issue>1</issue><spage>45</spage><epage>55</epage><pages>45-55</pages><issn>1880-1323</issn><eissn>1881-4689</eissn><abstract>DNA typing of samples obtained from crime scenes can be used to identify suspects. However, samples obtained from sex crimes are often mixtures, and it is difficult to separate these for DNA typing. We extracted sperm from mixed samples using an ultra-high-speed cell sorter and examined whether DNA typing was possible. Propidium iodide was able to react with semen, allowing sperm nuclei to be sorted with the cell sorter. DNA typing was performed using the sorted sperm. The utility of anti-sperm antibody for cell sorting was compared with that of anti-sperm surface protein SP17, and anti-sperm antibody was found to be more suitable. Thus, semen reacted with anti-sperm antibody and with propidium iodide, thereby allowing sperm with nuclei to be collected. 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| ispartof | Japanese Journal of Forensic Science and Technology, 2014, Vol.19(1), pp.45-55 |
| issn | 1880-1323 1881-4689 |
| language | eng |
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| source | J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese |
| subjects | Anti-sperm antibody Antibodies Cell sorter Crime Deoxyribonucleic acid Forensic science Iodides Mixed sample Nuclei Propidium iodide Semen Sperm Utilities |
| title | Separation of sperm from mixed samples using ultra-high-speed cell sorter followed by DNA typing |
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