Single molecule experimentation in biological physics: exploring the living component of soft condensed matter one molecule at a time
The soft matter of biological systems consists of mesoscopic length scale building blocks, composed of a variety of different types of biological molecules. Most single biological molecules are so small that 1 billion would fit on the full-stop at the end of this sentence, but collectively they carr...
Gespeichert in:
Veröffentlicht in: | Journal of physics. Condensed matter 2011-12, Vol.23 (50), p.503101-17 |
---|---|
Hauptverfasser: | , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 17 |
---|---|
container_issue | 50 |
container_start_page | 503101 |
container_title | Journal of physics. Condensed matter |
container_volume | 23 |
creator | Harriman, O L J Leake, M C |
description | The soft matter of biological systems consists of mesoscopic length scale building blocks, composed of a variety of different types of biological molecules. Most single biological molecules are so small that 1 billion would fit on the full-stop at the end of this sentence, but collectively they carry out the vital activities in living cells whose length scale is at least three orders of magnitude greater. Typically, the number of molecules involved in any given cellular process at any one time is relatively small, and so real physiological events may often be dominated by stochastics and fluctuation behaviour at levels comparable to thermal noise, and are generally heterogeneous in nature. This challenging combination of heterogeneity and stochasticity is best investigated experimentally at the level of single molecules, as opposed to more conventional bulk ensemble-average techniques. In recent years, the use of such molecular experimental approaches has become significantly more widespread in research laboratories around the world. In this review we discuss recent experimental approaches in biological physics which can be applied to investigate the living component of soft condensed matter to a precision of a single molecule. |
doi_str_mv | 10.1088/0953-8984/23/50/503101 |
format | Article |
fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_1671550696</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1671550696</sourcerecordid><originalsourceid>FETCH-LOGICAL-c453t-42f1e2c7f02af2c612ca2635eb833ce0c9dd20cf44b9ef5db1c9837a216b17823</originalsourceid><addsrcrecordid>eNqNkc9qFTEUxoMo9rb6CiUb0c1482eSybiTolUodGEL7kImc9JGMsk4yRX7AL63Ge61XShFOHAOye_LF86H0CklbylRakt6wRvVq3bL-FaQWpwS-gRtKJe0ka36-hRt7qEjdJzzN0JIq3j7HB0xRmQnRb9Bv774eBMATymA3dUBfs6w-AliMcWniH3Eg08h3XhrAp5v77K3-d2KhbRULS63gIP_sY42TXOKVYqTwzm5Uk_iCDHDiCdTCiy4Xj94mYINLtXsBXrmTMjw8tBP0PXHD1dnn5qLy_PPZ-8vGtsKXpqWOQrMdo4w45iVlFnDJBcwKM4tENuPIyPWte3QgxPjQG2veGcYlQPtFOMn6PX-3XlJ33eQi558thCCiZB2WfeUc6qIopV88yhJZUeFILKXFZV71C4p5wWcnusCzXKnKdFrWHrNQa85aMa1IHofVhWeHjx2wwTjvexPOhV4dQBMrtt3i4nW5wdO1N9ySSpH95xP8_-bN39r_s3qeXT8N-oUuxw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1671550696</pqid></control><display><type>article</type><title>Single molecule experimentation in biological physics: exploring the living component of soft condensed matter one molecule at a time</title><source>MEDLINE</source><source>HEAL-Link subscriptions: Institute of Physics (IOP) Journals</source><source>Institute of Physics Journals</source><creator>Harriman, O L J ; Leake, M C</creator><creatorcontrib>Harriman, O L J ; Leake, M C</creatorcontrib><description>The soft matter of biological systems consists of mesoscopic length scale building blocks, composed of a variety of different types of biological molecules. Most single biological molecules are so small that 1 billion would fit on the full-stop at the end of this sentence, but collectively they carry out the vital activities in living cells whose length scale is at least three orders of magnitude greater. Typically, the number of molecules involved in any given cellular process at any one time is relatively small, and so real physiological events may often be dominated by stochastics and fluctuation behaviour at levels comparable to thermal noise, and are generally heterogeneous in nature. This challenging combination of heterogeneity and stochasticity is best investigated experimentally at the level of single molecules, as opposed to more conventional bulk ensemble-average techniques. In recent years, the use of such molecular experimental approaches has become significantly more widespread in research laboratories around the world. In this review we discuss recent experimental approaches in biological physics which can be applied to investigate the living component of soft condensed matter to a precision of a single molecule.</description><identifier>ISSN: 0953-8984</identifier><identifier>EISSN: 1361-648X</identifier><identifier>DOI: 10.1088/0953-8984/23/50/503101</identifier><identifier>PMID: 22067659</identifier><identifier>CODEN: JCOMEL</identifier><language>eng</language><publisher>Bristol: IOP Publishing</publisher><subject>Animals ; Biological ; Biological and medical sciences ; Biophysics - methods ; Condensed matter ; Experimentation ; Fluctuation ; Fundamental and applied biological sciences. Psychology ; General aspects ; Heterogeneity ; Molecular biophysics ; Probability theory ; Sentences ; Spectrometry, Fluorescence ; Thermal noise</subject><ispartof>Journal of physics. Condensed matter, 2011-12, Vol.23 (50), p.503101-17</ispartof><rights>2015 INIST-CNRS</rights><rights>2011 IOP Publishing Ltd Printed in the UK & the USA</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-42f1e2c7f02af2c612ca2635eb833ce0c9dd20cf44b9ef5db1c9837a216b17823</citedby><cites>FETCH-LOGICAL-c453t-42f1e2c7f02af2c612ca2635eb833ce0c9dd20cf44b9ef5db1c9837a216b17823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://iopscience.iop.org/article/10.1088/0953-8984/23/50/503101/pdf$$EPDF$$P50$$Giop$$H</linktopdf><link.rule.ids>314,780,784,27924,27925,53830,53910</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25331360$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22067659$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Harriman, O L J</creatorcontrib><creatorcontrib>Leake, M C</creatorcontrib><title>Single molecule experimentation in biological physics: exploring the living component of soft condensed matter one molecule at a time</title><title>Journal of physics. Condensed matter</title><addtitle>J Phys Condens Matter</addtitle><description>The soft matter of biological systems consists of mesoscopic length scale building blocks, composed of a variety of different types of biological molecules. Most single biological molecules are so small that 1 billion would fit on the full-stop at the end of this sentence, but collectively they carry out the vital activities in living cells whose length scale is at least three orders of magnitude greater. Typically, the number of molecules involved in any given cellular process at any one time is relatively small, and so real physiological events may often be dominated by stochastics and fluctuation behaviour at levels comparable to thermal noise, and are generally heterogeneous in nature. This challenging combination of heterogeneity and stochasticity is best investigated experimentally at the level of single molecules, as opposed to more conventional bulk ensemble-average techniques. In recent years, the use of such molecular experimental approaches has become significantly more widespread in research laboratories around the world. In this review we discuss recent experimental approaches in biological physics which can be applied to investigate the living component of soft condensed matter to a precision of a single molecule.</description><subject>Animals</subject><subject>Biological</subject><subject>Biological and medical sciences</subject><subject>Biophysics - methods</subject><subject>Condensed matter</subject><subject>Experimentation</subject><subject>Fluctuation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Heterogeneity</subject><subject>Molecular biophysics</subject><subject>Probability theory</subject><subject>Sentences</subject><subject>Spectrometry, Fluorescence</subject><subject>Thermal noise</subject><issn>0953-8984</issn><issn>1361-648X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc9qFTEUxoMo9rb6CiUb0c1482eSybiTolUodGEL7kImc9JGMsk4yRX7AL63Ge61XShFOHAOye_LF86H0CklbylRakt6wRvVq3bL-FaQWpwS-gRtKJe0ka36-hRt7qEjdJzzN0JIq3j7HB0xRmQnRb9Bv774eBMATymA3dUBfs6w-AliMcWniH3Eg08h3XhrAp5v77K3-d2KhbRULS63gIP_sY42TXOKVYqTwzm5Uk_iCDHDiCdTCiy4Xj94mYINLtXsBXrmTMjw8tBP0PXHD1dnn5qLy_PPZ-8vGtsKXpqWOQrMdo4w45iVlFnDJBcwKM4tENuPIyPWte3QgxPjQG2veGcYlQPtFOMn6PX-3XlJ33eQi558thCCiZB2WfeUc6qIopV88yhJZUeFILKXFZV71C4p5wWcnusCzXKnKdFrWHrNQa85aMa1IHofVhWeHjx2wwTjvexPOhV4dQBMrtt3i4nW5wdO1N9ySSpH95xP8_-bN39r_s3qeXT8N-oUuxw</recordid><startdate>20111221</startdate><enddate>20111221</enddate><creator>Harriman, O L J</creator><creator>Leake, M C</creator><general>IOP Publishing</general><general>Institute of Physics</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20111221</creationdate><title>Single molecule experimentation in biological physics: exploring the living component of soft condensed matter one molecule at a time</title><author>Harriman, O L J ; Leake, M C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-42f1e2c7f02af2c612ca2635eb833ce0c9dd20cf44b9ef5db1c9837a216b17823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Biological</topic><topic>Biological and medical sciences</topic><topic>Biophysics - methods</topic><topic>Condensed matter</topic><topic>Experimentation</topic><topic>Fluctuation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>Heterogeneity</topic><topic>Molecular biophysics</topic><topic>Probability theory</topic><topic>Sentences</topic><topic>Spectrometry, Fluorescence</topic><topic>Thermal noise</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Harriman, O L J</creatorcontrib><creatorcontrib>Leake, M C</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of physics. Condensed matter</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Harriman, O L J</au><au>Leake, M C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Single molecule experimentation in biological physics: exploring the living component of soft condensed matter one molecule at a time</atitle><jtitle>Journal of physics. Condensed matter</jtitle><addtitle>J Phys Condens Matter</addtitle><date>2011-12-21</date><risdate>2011</risdate><volume>23</volume><issue>50</issue><spage>503101</spage><epage>17</epage><pages>503101-17</pages><issn>0953-8984</issn><eissn>1361-648X</eissn><coden>JCOMEL</coden><abstract>The soft matter of biological systems consists of mesoscopic length scale building blocks, composed of a variety of different types of biological molecules. Most single biological molecules are so small that 1 billion would fit on the full-stop at the end of this sentence, but collectively they carry out the vital activities in living cells whose length scale is at least three orders of magnitude greater. Typically, the number of molecules involved in any given cellular process at any one time is relatively small, and so real physiological events may often be dominated by stochastics and fluctuation behaviour at levels comparable to thermal noise, and are generally heterogeneous in nature. This challenging combination of heterogeneity and stochasticity is best investigated experimentally at the level of single molecules, as opposed to more conventional bulk ensemble-average techniques. In recent years, the use of such molecular experimental approaches has become significantly more widespread in research laboratories around the world. In this review we discuss recent experimental approaches in biological physics which can be applied to investigate the living component of soft condensed matter to a precision of a single molecule.</abstract><cop>Bristol</cop><pub>IOP Publishing</pub><pmid>22067659</pmid><doi>10.1088/0953-8984/23/50/503101</doi><tpages>17</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0953-8984 |
ispartof | Journal of physics. Condensed matter, 2011-12, Vol.23 (50), p.503101-17 |
issn | 0953-8984 1361-648X |
language | eng |
recordid | cdi_proquest_miscellaneous_1671550696 |
source | MEDLINE; HEAL-Link subscriptions: Institute of Physics (IOP) Journals; Institute of Physics Journals |
subjects | Animals Biological Biological and medical sciences Biophysics - methods Condensed matter Experimentation Fluctuation Fundamental and applied biological sciences. Psychology General aspects Heterogeneity Molecular biophysics Probability theory Sentences Spectrometry, Fluorescence Thermal noise |
title | Single molecule experimentation in biological physics: exploring the living component of soft condensed matter one molecule at a time |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T08%3A22%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Single%20molecule%20experimentation%20in%20biological%20physics:%20exploring%20the%20living%20component%20of%20soft%20condensed%20matter%20one%20molecule%20at%20a%20time&rft.jtitle=Journal%20of%20physics.%20Condensed%20matter&rft.au=Harriman,%20O%20L%20J&rft.date=2011-12-21&rft.volume=23&rft.issue=50&rft.spage=503101&rft.epage=17&rft.pages=503101-17&rft.issn=0953-8984&rft.eissn=1361-648X&rft.coden=JCOMEL&rft_id=info:doi/10.1088/0953-8984/23/50/503101&rft_dat=%3Cproquest_pubme%3E1671550696%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1671550696&rft_id=info:pmid/22067659&rfr_iscdi=true |