A novel cross-linked human amniotic membrane for corneal implantations
The aim of this study was to evaluate the efficacy of Al2(SO4)3 cross-linked human amniotic membrane for ocular surface reconstruction using tissue culture techniques. The human amniotic membrane was cross-linked with Al2(SO4)3, and the cross-linked human amniotic membrane was characterized for its...
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| Veröffentlicht in: | Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine Journal of engineering in medicine, 2013-03, Vol.227 (3), p.221-228 |
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| container_title | Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine |
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| creator | Sekar, S Sasirekha, K Krishnakumar, S Sastry, TP |
| description | The aim of this study was to evaluate the efficacy of Al2(SO4)3 cross-linked human amniotic membrane for ocular surface reconstruction using tissue culture techniques. The human amniotic membrane was cross-linked with Al2(SO4)3, and the cross-linked human amniotic membrane was characterized for its mechanical properties, percentage of swelling in water, sterility, infrared spectroscopy and scanning electron microscopy. The potential of cross-linked human amniotic membrane to support the attachment and proliferation of corneal limbal epithelial cells was assessed in vitro, using static culture system. About 125% increase in the tensile strength was observed in the cross-linked human amniotic membrane compared to human amniotic membrane. Infrared spectroscopy studies have confirmed the cross-linking of human amniotic membrane with Al2(SO4)3. The cross-linked human amniotic membrane was found to be sterile up to 1 year. In culture studies, confluent sheets of epithelial cells were seen at the end of 14th day resembling the morphological features of limbal epithelia. The cross-linked human amniotic membrane has exhibited improved mechanical properties, and the tissue culture studies have shown its feasibility to be used as a limbal transplant. It was concluded that the cross-linked human amniotic membrane with its improved mechanical properties could be used on par with human amniotic membrane. |
| doi_str_mv | 10.1177/0954411912472423 |
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The human amniotic membrane was cross-linked with Al2(SO4)3, and the cross-linked human amniotic membrane was characterized for its mechanical properties, percentage of swelling in water, sterility, infrared spectroscopy and scanning electron microscopy. The potential of cross-linked human amniotic membrane to support the attachment and proliferation of corneal limbal epithelial cells was assessed in vitro, using static culture system. About 125% increase in the tensile strength was observed in the cross-linked human amniotic membrane compared to human amniotic membrane. Infrared spectroscopy studies have confirmed the cross-linking of human amniotic membrane with Al2(SO4)3. The cross-linked human amniotic membrane was found to be sterile up to 1 year. In culture studies, confluent sheets of epithelial cells were seen at the end of 14th day resembling the morphological features of limbal epithelia. The cross-linked human amniotic membrane has exhibited improved mechanical properties, and the tissue culture studies have shown its feasibility to be used as a limbal transplant. It was concluded that the cross-linked human amniotic membrane with its improved mechanical properties could be used on par with human amniotic membrane.</description><identifier>ISSN: 0954-4119</identifier><identifier>EISSN: 2041-3033</identifier><identifier>DOI: 10.1177/0954411912472423</identifier><identifier>PMID: 23662337</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Alum Compounds - chemistry ; Amnion - chemistry ; Biomechanical Phenomena ; Cells ; Cells, Cultured ; Cornea ; Corneal Transplantation - methods ; Cross-Linking Reagents - chemistry ; Crosslinking ; Culture ; Culture techniques ; Epithelial Cells - cytology ; Human ; Humans ; Infrared spectroscopy ; Limbus Corneae - cytology ; Mechanical properties ; Membranes ; Reconstruction ; Scanning electron microscopy ; Tensile Strength ; Tissue Culture Techniques ; Tissue Engineering - methods ; Tissue Scaffolds - chemistry ; Tissues ; Transplants & implants</subject><ispartof>Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine, 2013-03, Vol.227 (3), p.221-228</ispartof><rights>IMechE 2012</rights><rights>Copyright SAGE PUBLICATIONS, INC. Mar 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-1f7a0566866877cb9c2b6ce51e27db017fc650b5d8698a659955d9f3457356c93</citedby><cites>FETCH-LOGICAL-c431t-1f7a0566866877cb9c2b6ce51e27db017fc650b5d8698a659955d9f3457356c93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/0954411912472423$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/0954411912472423$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,21798,27901,27902,43597,43598</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23662337$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sekar, S</creatorcontrib><creatorcontrib>Sasirekha, K</creatorcontrib><creatorcontrib>Krishnakumar, S</creatorcontrib><creatorcontrib>Sastry, TP</creatorcontrib><title>A novel cross-linked human amniotic membrane for corneal implantations</title><title>Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine</title><addtitle>Proc Inst Mech Eng H</addtitle><description>The aim of this study was to evaluate the efficacy of Al2(SO4)3 cross-linked human amniotic membrane for ocular surface reconstruction using tissue culture techniques. The human amniotic membrane was cross-linked with Al2(SO4)3, and the cross-linked human amniotic membrane was characterized for its mechanical properties, percentage of swelling in water, sterility, infrared spectroscopy and scanning electron microscopy. The potential of cross-linked human amniotic membrane to support the attachment and proliferation of corneal limbal epithelial cells was assessed in vitro, using static culture system. About 125% increase in the tensile strength was observed in the cross-linked human amniotic membrane compared to human amniotic membrane. Infrared spectroscopy studies have confirmed the cross-linking of human amniotic membrane with Al2(SO4)3. The cross-linked human amniotic membrane was found to be sterile up to 1 year. In culture studies, confluent sheets of epithelial cells were seen at the end of 14th day resembling the morphological features of limbal epithelia. The cross-linked human amniotic membrane has exhibited improved mechanical properties, and the tissue culture studies have shown its feasibility to be used as a limbal transplant. It was concluded that the cross-linked human amniotic membrane with its improved mechanical properties could be used on par with human amniotic membrane.</description><subject>Alum Compounds - chemistry</subject><subject>Amnion - chemistry</subject><subject>Biomechanical Phenomena</subject><subject>Cells</subject><subject>Cells, Cultured</subject><subject>Cornea</subject><subject>Corneal Transplantation - methods</subject><subject>Cross-Linking Reagents - chemistry</subject><subject>Crosslinking</subject><subject>Culture</subject><subject>Culture techniques</subject><subject>Epithelial Cells - cytology</subject><subject>Human</subject><subject>Humans</subject><subject>Infrared spectroscopy</subject><subject>Limbus Corneae - cytology</subject><subject>Mechanical properties</subject><subject>Membranes</subject><subject>Reconstruction</subject><subject>Scanning electron microscopy</subject><subject>Tensile Strength</subject><subject>Tissue Culture Techniques</subject><subject>Tissue Engineering - methods</subject><subject>Tissue Scaffolds - chemistry</subject><subject>Tissues</subject><subject>Transplants & implants</subject><issn>0954-4119</issn><issn>2041-3033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkctLxDAQxoMouj7unqTgxUt1Js_mKOKqIHjRc0nTVKttsiat4H9v11URQRQG5jC_-ebxEbKPcIyo1AlowTmiRsoV5ZStkRkFjjkDxtbJbFnOl_Utsp3SIwAggtwkW5RJSRlTMzI_zXx4cV1mY0gp71r_5OrsYeyNz0zv2zC0NutdX0XjXdaEmNkQvTNd1vaLzvjBDG3waZdsNKZLbu8j75C7-fnt2WV-fXNxdXZ6nVvOcMixUQaElMUUStlKW1pJ6wQ6quoKUDVWCqhEXUhdGCm0FqLWDeNCMSGtZjvkaKW7iOF5dGko-zZZ102buDCmEqVCwbRgxd8oE4CCak3_gXLNOaBkE3r4A30MY_TTzROFinEoQE0UrKj3r0bXlIvY9ia-lgjl0rnyp3NTy8GH8Fj1rv5q-LRqAvIVkMy9-zb1N8E3k6qctA</recordid><startdate>201303</startdate><enddate>201303</enddate><creator>Sekar, S</creator><creator>Sasirekha, K</creator><creator>Krishnakumar, S</creator><creator>Sastry, TP</creator><general>SAGE Publications</general><general>SAGE PUBLICATIONS, INC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201303</creationdate><title>A novel cross-linked human amniotic membrane for corneal implantations</title><author>Sekar, S ; Sasirekha, K ; Krishnakumar, S ; Sastry, TP</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-1f7a0566866877cb9c2b6ce51e27db017fc650b5d8698a659955d9f3457356c93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Alum Compounds - chemistry</topic><topic>Amnion - chemistry</topic><topic>Biomechanical Phenomena</topic><topic>Cells</topic><topic>Cells, Cultured</topic><topic>Cornea</topic><topic>Corneal Transplantation - methods</topic><topic>Cross-Linking Reagents - chemistry</topic><topic>Crosslinking</topic><topic>Culture</topic><topic>Culture techniques</topic><topic>Epithelial Cells - cytology</topic><topic>Human</topic><topic>Humans</topic><topic>Infrared spectroscopy</topic><topic>Limbus Corneae - cytology</topic><topic>Mechanical properties</topic><topic>Membranes</topic><topic>Reconstruction</topic><topic>Scanning electron microscopy</topic><topic>Tensile Strength</topic><topic>Tissue Culture Techniques</topic><topic>Tissue Engineering - methods</topic><topic>Tissue Scaffolds - chemistry</topic><topic>Tissues</topic><topic>Transplants & implants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sekar, S</creatorcontrib><creatorcontrib>Sasirekha, K</creatorcontrib><creatorcontrib>Krishnakumar, S</creatorcontrib><creatorcontrib>Sastry, TP</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sekar, S</au><au>Sasirekha, K</au><au>Krishnakumar, S</au><au>Sastry, TP</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel cross-linked human amniotic membrane for corneal implantations</atitle><jtitle>Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine</jtitle><addtitle>Proc Inst Mech Eng H</addtitle><date>2013-03</date><risdate>2013</risdate><volume>227</volume><issue>3</issue><spage>221</spage><epage>228</epage><pages>221-228</pages><issn>0954-4119</issn><eissn>2041-3033</eissn><abstract>The aim of this study was to evaluate the efficacy of Al2(SO4)3 cross-linked human amniotic membrane for ocular surface reconstruction using tissue culture techniques. The human amniotic membrane was cross-linked with Al2(SO4)3, and the cross-linked human amniotic membrane was characterized for its mechanical properties, percentage of swelling in water, sterility, infrared spectroscopy and scanning electron microscopy. The potential of cross-linked human amniotic membrane to support the attachment and proliferation of corneal limbal epithelial cells was assessed in vitro, using static culture system. About 125% increase in the tensile strength was observed in the cross-linked human amniotic membrane compared to human amniotic membrane. Infrared spectroscopy studies have confirmed the cross-linking of human amniotic membrane with Al2(SO4)3. The cross-linked human amniotic membrane was found to be sterile up to 1 year. In culture studies, confluent sheets of epithelial cells were seen at the end of 14th day resembling the morphological features of limbal epithelia. The cross-linked human amniotic membrane has exhibited improved mechanical properties, and the tissue culture studies have shown its feasibility to be used as a limbal transplant. It was concluded that the cross-linked human amniotic membrane with its improved mechanical properties could be used on par with human amniotic membrane.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>23662337</pmid><doi>10.1177/0954411912472423</doi><tpages>8</tpages></addata></record> |
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| ispartof | Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine, 2013-03, Vol.227 (3), p.221-228 |
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| subjects | Alum Compounds - chemistry Amnion - chemistry Biomechanical Phenomena Cells Cells, Cultured Cornea Corneal Transplantation - methods Cross-Linking Reagents - chemistry Crosslinking Culture Culture techniques Epithelial Cells - cytology Human Humans Infrared spectroscopy Limbus Corneae - cytology Mechanical properties Membranes Reconstruction Scanning electron microscopy Tensile Strength Tissue Culture Techniques Tissue Engineering - methods Tissue Scaffolds - chemistry Tissues Transplants & implants |
| title | A novel cross-linked human amniotic membrane for corneal implantations |
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