The microalga Parachlorella kessleri--A novel highly efficient lipid producer
The alga Parachlorella kessleri, strain CCALA 255, grown under optimal conditions, is characterized by storage of energy in the form of starch rather than lipids. If grown in the complete medium, the cultures grew rapidly, producing large amounts of biomass in a relatively short time. The cells, how...
Gespeichert in:
| Veröffentlicht in: | Biotechnology and bioengineering 2013-01, Vol.110 (1), p.97-107 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 107 |
|---|---|
| container_issue | 1 |
| container_start_page | 97 |
| container_title | Biotechnology and bioengineering |
| container_volume | 110 |
| creator | Li, Xiuling Přibyl, Pavel Bišová, Kateřina Kawano, Shigeyuki Cepák, Vladislav Zachleder, Vilém Čížková, Mária Brányiková, Irena Vítová, Milada |
| description | The alga Parachlorella kessleri, strain CCALA 255, grown under optimal conditions, is characterized by storage of energy in the form of starch rather than lipids. If grown in the complete medium, the cultures grew rapidly, producing large amounts of biomass in a relatively short time. The cells, however, contained negligible lipid reserves (1–10% of DW). Treatments inducing hyperproduction of storage lipids in P. kessleri biomass were described. The cultures were grown in the absence or fivefold decreased concentration of either nitrogen or phosphorus or sulfur. Limitation by all elements using fivefold or 10‐fold diluted mineral medium was also tested. Limitation with any macroelement (nitrogen, sulfur, or phosphorus) led to an increase in the amount of lipids; nitrogen limitation was the most effective. Diluted nutrient media (5‐ or 10‐fold) were identified as the best method to stimulate lipid overproduction (60% of DW). The strategy for lipid overproduction consists of the fast growth of P. kessleri culture grown in the complete medium to produce sufficient biomass (DW more than 10 g/L) followed by the dilution of nutrient medium to stop growth and cell division by limitation of all elements, leading to induction of lipid production and accumulation up to 60% DW. Cultivation conditions necessary for maximizing lipid content in P. kessleri biomass generated in a scale‐up solar open thin‐layer photobioreactor were described. Biotechnol. Bioeng. 2013; 110: 97–107. © 2012 Wiley Periodicals, Inc.
Limitation with any macroelement or depletion of mineral medium stimulated lipid overproduction in the alga Parachlorella kessleri. The strategy for industrial application consists of the fast growth of culture grown in the complete medium to produce sufficient biomass (DW more than 10 g/L) followed by the dilution of nutrient medium to stop growth and cell division by limitation of all elements, leading to induction of lipid production and accumulation up to 60% DW. |
| doi_str_mv | 10.1002/bit.24595 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1671519688</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1434025231</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5235-85f661bebd809411578b1874f2842d117bbcdebea20fb0ab89f77af6fa0406c3</originalsourceid><addsrcrecordid>eNqF0U9PFDEYBvCGYGBFD3wBMgkXPQy0nf49ItGVBBdNJtFb0868ZQvdnbXdUffb23WBgwnh1DT59enbPggdE3xGMKbnLqzPKOOa76EJwVrWmGq8jyYYY1E3XNND9Drnu7KVSogDdEipFEIyPUFf2jlUi9ClwcZbW321yXbzOCSI0Vb3kHOEFOr6oloOvyBW83A7j5sKvA9dgOW6imEV-mqVhn7sIL1Br7yNGd4-rEeo_fSxvfxcX99Mry4vruuO04bXinshiAPXK6wZIVwqR5RknipGe0Kkc10PDizF3mHrlPZSWi-8xQyLrjlC73ax5d6fI-S1WYTcbUdewjBmQ4QknGih1MuUNQzTMhV5mRIlNFVlxkJP_6N3w5iW5clbpShhSuOi3u9U-d2cE3izSmFh08YQbLbFmVKc-VdcsScPiaNbQP8kH5sq4HwHfocIm-eTzIer9jGy3p0IeQ1_nk7YdG-EbCQ332dTw9iP2aydUvOt-Qsf5K9E</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1188214890</pqid></control><display><type>article</type><title>The microalga Parachlorella kessleri--A novel highly efficient lipid producer</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Li, Xiuling ; Přibyl, Pavel ; Bišová, Kateřina ; Kawano, Shigeyuki ; Cepák, Vladislav ; Zachleder, Vilém ; Čížková, Mária ; Brányiková, Irena ; Vítová, Milada</creator><creatorcontrib>Li, Xiuling ; Přibyl, Pavel ; Bišová, Kateřina ; Kawano, Shigeyuki ; Cepák, Vladislav ; Zachleder, Vilém ; Čížková, Mária ; Brányiková, Irena ; Vítová, Milada</creatorcontrib><description>The alga Parachlorella kessleri, strain CCALA 255, grown under optimal conditions, is characterized by storage of energy in the form of starch rather than lipids. If grown in the complete medium, the cultures grew rapidly, producing large amounts of biomass in a relatively short time. The cells, however, contained negligible lipid reserves (1–10% of DW). Treatments inducing hyperproduction of storage lipids in P. kessleri biomass were described. The cultures were grown in the absence or fivefold decreased concentration of either nitrogen or phosphorus or sulfur. Limitation by all elements using fivefold or 10‐fold diluted mineral medium was also tested. Limitation with any macroelement (nitrogen, sulfur, or phosphorus) led to an increase in the amount of lipids; nitrogen limitation was the most effective. Diluted nutrient media (5‐ or 10‐fold) were identified as the best method to stimulate lipid overproduction (60% of DW). The strategy for lipid overproduction consists of the fast growth of P. kessleri culture grown in the complete medium to produce sufficient biomass (DW more than 10 g/L) followed by the dilution of nutrient medium to stop growth and cell division by limitation of all elements, leading to induction of lipid production and accumulation up to 60% DW. Cultivation conditions necessary for maximizing lipid content in P. kessleri biomass generated in a scale‐up solar open thin‐layer photobioreactor were described. Biotechnol. Bioeng. 2013; 110: 97–107. © 2012 Wiley Periodicals, Inc.
Limitation with any macroelement or depletion of mineral medium stimulated lipid overproduction in the alga Parachlorella kessleri. The strategy for industrial application consists of the fast growth of culture grown in the complete medium to produce sufficient biomass (DW more than 10 g/L) followed by the dilution of nutrient medium to stop growth and cell division by limitation of all elements, leading to induction of lipid production and accumulation up to 60% DW.</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.24595</identifier><identifier>PMID: 22766749</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Algae ; Biomass ; Biotechnology ; carbon dioxide ; Carbon Dioxide - metabolism ; Cell division ; Chlorophyll - analysis ; Chlorophyll - metabolism ; Chlorophyta - metabolism ; Culture ; Culture Media ; Dilution ; Fatty Acids - analysis ; Fatty Acids - metabolism ; light intensity ; limitation by elements ; lipid hyperproduction ; Lipid Metabolism ; Lipids ; Lipids - biosynthesis ; Microalgae - metabolism ; Nitrogen ; Nutrients ; Parachlorella kessleri ; Phosphorus ; Photobioreactors ; Reserves ; Starch - analysis ; Starch - metabolism ; Sulfur ; thin-layer photobioreactor</subject><ispartof>Biotechnology and bioengineering, 2013-01, Vol.110 (1), p.97-107</ispartof><rights>Copyright © 2012 Wiley Periodicals, Inc.</rights><rights>Copyright John Wiley and Sons, Limited Jan 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5235-85f661bebd809411578b1874f2842d117bbcdebea20fb0ab89f77af6fa0406c3</citedby><cites>FETCH-LOGICAL-c5235-85f661bebd809411578b1874f2842d117bbcdebea20fb0ab89f77af6fa0406c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.24595$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.24595$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22766749$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Xiuling</creatorcontrib><creatorcontrib>Přibyl, Pavel</creatorcontrib><creatorcontrib>Bišová, Kateřina</creatorcontrib><creatorcontrib>Kawano, Shigeyuki</creatorcontrib><creatorcontrib>Cepák, Vladislav</creatorcontrib><creatorcontrib>Zachleder, Vilém</creatorcontrib><creatorcontrib>Čížková, Mária</creatorcontrib><creatorcontrib>Brányiková, Irena</creatorcontrib><creatorcontrib>Vítová, Milada</creatorcontrib><title>The microalga Parachlorella kessleri--A novel highly efficient lipid producer</title><title>Biotechnology and bioengineering</title><addtitle>Biotechnol. Bioeng</addtitle><description>The alga Parachlorella kessleri, strain CCALA 255, grown under optimal conditions, is characterized by storage of energy in the form of starch rather than lipids. If grown in the complete medium, the cultures grew rapidly, producing large amounts of biomass in a relatively short time. The cells, however, contained negligible lipid reserves (1–10% of DW). Treatments inducing hyperproduction of storage lipids in P. kessleri biomass were described. The cultures were grown in the absence or fivefold decreased concentration of either nitrogen or phosphorus or sulfur. Limitation by all elements using fivefold or 10‐fold diluted mineral medium was also tested. Limitation with any macroelement (nitrogen, sulfur, or phosphorus) led to an increase in the amount of lipids; nitrogen limitation was the most effective. Diluted nutrient media (5‐ or 10‐fold) were identified as the best method to stimulate lipid overproduction (60% of DW). The strategy for lipid overproduction consists of the fast growth of P. kessleri culture grown in the complete medium to produce sufficient biomass (DW more than 10 g/L) followed by the dilution of nutrient medium to stop growth and cell division by limitation of all elements, leading to induction of lipid production and accumulation up to 60% DW. Cultivation conditions necessary for maximizing lipid content in P. kessleri biomass generated in a scale‐up solar open thin‐layer photobioreactor were described. Biotechnol. Bioeng. 2013; 110: 97–107. © 2012 Wiley Periodicals, Inc.
Limitation with any macroelement or depletion of mineral medium stimulated lipid overproduction in the alga Parachlorella kessleri. The strategy for industrial application consists of the fast growth of culture grown in the complete medium to produce sufficient biomass (DW more than 10 g/L) followed by the dilution of nutrient medium to stop growth and cell division by limitation of all elements, leading to induction of lipid production and accumulation up to 60% DW.</description><subject>Algae</subject><subject>Biomass</subject><subject>Biotechnology</subject><subject>carbon dioxide</subject><subject>Carbon Dioxide - metabolism</subject><subject>Cell division</subject><subject>Chlorophyll - analysis</subject><subject>Chlorophyll - metabolism</subject><subject>Chlorophyta - metabolism</subject><subject>Culture</subject><subject>Culture Media</subject><subject>Dilution</subject><subject>Fatty Acids - analysis</subject><subject>Fatty Acids - metabolism</subject><subject>light intensity</subject><subject>limitation by elements</subject><subject>lipid hyperproduction</subject><subject>Lipid Metabolism</subject><subject>Lipids</subject><subject>Lipids - biosynthesis</subject><subject>Microalgae - metabolism</subject><subject>Nitrogen</subject><subject>Nutrients</subject><subject>Parachlorella kessleri</subject><subject>Phosphorus</subject><subject>Photobioreactors</subject><subject>Reserves</subject><subject>Starch - analysis</subject><subject>Starch - metabolism</subject><subject>Sulfur</subject><subject>thin-layer photobioreactor</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U9PFDEYBvCGYGBFD3wBMgkXPQy0nf49ItGVBBdNJtFb0868ZQvdnbXdUffb23WBgwnh1DT59enbPggdE3xGMKbnLqzPKOOa76EJwVrWmGq8jyYYY1E3XNND9Drnu7KVSogDdEipFEIyPUFf2jlUi9ClwcZbW321yXbzOCSI0Vb3kHOEFOr6oloOvyBW83A7j5sKvA9dgOW6imEV-mqVhn7sIL1Br7yNGd4-rEeo_fSxvfxcX99Mry4vruuO04bXinshiAPXK6wZIVwqR5RknipGe0Kkc10PDizF3mHrlPZSWi-8xQyLrjlC73ax5d6fI-S1WYTcbUdewjBmQ4QknGih1MuUNQzTMhV5mRIlNFVlxkJP_6N3w5iW5clbpShhSuOi3u9U-d2cE3izSmFh08YQbLbFmVKc-VdcsScPiaNbQP8kH5sq4HwHfocIm-eTzIer9jGy3p0IeQ1_nk7YdG-EbCQ332dTw9iP2aydUvOt-Qsf5K9E</recordid><startdate>201301</startdate><enddate>201301</enddate><creator>Li, Xiuling</creator><creator>Přibyl, Pavel</creator><creator>Bišová, Kateřina</creator><creator>Kawano, Shigeyuki</creator><creator>Cepák, Vladislav</creator><creator>Zachleder, Vilém</creator><creator>Čížková, Mária</creator><creator>Brányiková, Irena</creator><creator>Vítová, Milada</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>H98</scope><scope>L.G</scope><scope>7SU</scope></search><sort><creationdate>201301</creationdate><title>The microalga Parachlorella kessleri--A novel highly efficient lipid producer</title><author>Li, Xiuling ; Přibyl, Pavel ; Bišová, Kateřina ; Kawano, Shigeyuki ; Cepák, Vladislav ; Zachleder, Vilém ; Čížková, Mária ; Brányiková, Irena ; Vítová, Milada</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5235-85f661bebd809411578b1874f2842d117bbcdebea20fb0ab89f77af6fa0406c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Algae</topic><topic>Biomass</topic><topic>Biotechnology</topic><topic>carbon dioxide</topic><topic>Carbon Dioxide - metabolism</topic><topic>Cell division</topic><topic>Chlorophyll - analysis</topic><topic>Chlorophyll - metabolism</topic><topic>Chlorophyta - metabolism</topic><topic>Culture</topic><topic>Culture Media</topic><topic>Dilution</topic><topic>Fatty Acids - analysis</topic><topic>Fatty Acids - metabolism</topic><topic>light intensity</topic><topic>limitation by elements</topic><topic>lipid hyperproduction</topic><topic>Lipid Metabolism</topic><topic>Lipids</topic><topic>Lipids - biosynthesis</topic><topic>Microalgae - metabolism</topic><topic>Nitrogen</topic><topic>Nutrients</topic><topic>Parachlorella kessleri</topic><topic>Phosphorus</topic><topic>Photobioreactors</topic><topic>Reserves</topic><topic>Starch - analysis</topic><topic>Starch - metabolism</topic><topic>Sulfur</topic><topic>thin-layer photobioreactor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Xiuling</creatorcontrib><creatorcontrib>Přibyl, Pavel</creatorcontrib><creatorcontrib>Bišová, Kateřina</creatorcontrib><creatorcontrib>Kawano, Shigeyuki</creatorcontrib><creatorcontrib>Cepák, Vladislav</creatorcontrib><creatorcontrib>Zachleder, Vilém</creatorcontrib><creatorcontrib>Čížková, Mária</creatorcontrib><creatorcontrib>Brányiková, Irena</creatorcontrib><creatorcontrib>Vítová, Milada</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Environmental Engineering Abstracts</collection><jtitle>Biotechnology and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Xiuling</au><au>Přibyl, Pavel</au><au>Bišová, Kateřina</au><au>Kawano, Shigeyuki</au><au>Cepák, Vladislav</au><au>Zachleder, Vilém</au><au>Čížková, Mária</au><au>Brányiková, Irena</au><au>Vítová, Milada</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The microalga Parachlorella kessleri--A novel highly efficient lipid producer</atitle><jtitle>Biotechnology and bioengineering</jtitle><addtitle>Biotechnol. Bioeng</addtitle><date>2013-01</date><risdate>2013</risdate><volume>110</volume><issue>1</issue><spage>97</spage><epage>107</epage><pages>97-107</pages><issn>0006-3592</issn><eissn>1097-0290</eissn><coden>BIBIAU</coden><abstract>The alga Parachlorella kessleri, strain CCALA 255, grown under optimal conditions, is characterized by storage of energy in the form of starch rather than lipids. If grown in the complete medium, the cultures grew rapidly, producing large amounts of biomass in a relatively short time. The cells, however, contained negligible lipid reserves (1–10% of DW). Treatments inducing hyperproduction of storage lipids in P. kessleri biomass were described. The cultures were grown in the absence or fivefold decreased concentration of either nitrogen or phosphorus or sulfur. Limitation by all elements using fivefold or 10‐fold diluted mineral medium was also tested. Limitation with any macroelement (nitrogen, sulfur, or phosphorus) led to an increase in the amount of lipids; nitrogen limitation was the most effective. Diluted nutrient media (5‐ or 10‐fold) were identified as the best method to stimulate lipid overproduction (60% of DW). The strategy for lipid overproduction consists of the fast growth of P. kessleri culture grown in the complete medium to produce sufficient biomass (DW more than 10 g/L) followed by the dilution of nutrient medium to stop growth and cell division by limitation of all elements, leading to induction of lipid production and accumulation up to 60% DW. Cultivation conditions necessary for maximizing lipid content in P. kessleri biomass generated in a scale‐up solar open thin‐layer photobioreactor were described. Biotechnol. Bioeng. 2013; 110: 97–107. © 2012 Wiley Periodicals, Inc.
Limitation with any macroelement or depletion of mineral medium stimulated lipid overproduction in the alga Parachlorella kessleri. The strategy for industrial application consists of the fast growth of culture grown in the complete medium to produce sufficient biomass (DW more than 10 g/L) followed by the dilution of nutrient medium to stop growth and cell division by limitation of all elements, leading to induction of lipid production and accumulation up to 60% DW.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>22766749</pmid><doi>10.1002/bit.24595</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-3592 |
| ispartof | Biotechnology and bioengineering, 2013-01, Vol.110 (1), p.97-107 |
| issn | 0006-3592 1097-0290 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1671519688 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Algae Biomass Biotechnology carbon dioxide Carbon Dioxide - metabolism Cell division Chlorophyll - analysis Chlorophyll - metabolism Chlorophyta - metabolism Culture Culture Media Dilution Fatty Acids - analysis Fatty Acids - metabolism light intensity limitation by elements lipid hyperproduction Lipid Metabolism Lipids Lipids - biosynthesis Microalgae - metabolism Nitrogen Nutrients Parachlorella kessleri Phosphorus Photobioreactors Reserves Starch - analysis Starch - metabolism Sulfur thin-layer photobioreactor |
| title | The microalga Parachlorella kessleri--A novel highly efficient lipid producer |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T20%3A04%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20microalga%20Parachlorella%20kessleri--A%20novel%20highly%20efficient%20lipid%20producer&rft.jtitle=Biotechnology%20and%20bioengineering&rft.au=Li,%20Xiuling&rft.date=2013-01&rft.volume=110&rft.issue=1&rft.spage=97&rft.epage=107&rft.pages=97-107&rft.issn=0006-3592&rft.eissn=1097-0290&rft.coden=BIBIAU&rft_id=info:doi/10.1002/bit.24595&rft_dat=%3Cproquest_cross%3E1434025231%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1188214890&rft_id=info:pmid/22766749&rfr_iscdi=true |