Photoinduced electron transfer mechanism between green fluorescent protein molecules and metal oxide nanoparticles

Green fluorescent protein (GFP) molecules are attached to titanium dioxide and cadmium oxide nanoparticles via sol–gel method and fluorescence dynamics of such a protein–metal oxide assembly is investigated with a conventional time correlated single photon counting technique. As compared to free flu...

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Veröffentlicht in:Ceramics international 2014-03, Vol.40 (2), p.2943-2951
Hauptverfasser: Acikgoz, Sabriye, Ulusu, Yakup, Akin, Seckin, Sonmezoglu, Savas, Gokce, Isa, Inci, Mehmet Naci
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container_issue 2
container_start_page 2943
container_title Ceramics international
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creator Acikgoz, Sabriye
Ulusu, Yakup
Akin, Seckin
Sonmezoglu, Savas
Gokce, Isa
Inci, Mehmet Naci
description Green fluorescent protein (GFP) molecules are attached to titanium dioxide and cadmium oxide nanoparticles via sol–gel method and fluorescence dynamics of such a protein–metal oxide assembly is investigated with a conventional time correlated single photon counting technique. As compared to free fluorescent protein molecules, time-resolved experiments show that the fluorescence lifetime of GFP molecules bound to these metal oxide nanoparticles gets shortened dramatically. Such a decrease in the lifetime is measured to be 22 and 43 percent for cadmium oxide and titanium dioxide respectively, which is due to photoinduced electron transfer mechanism caused by the interaction of GFP molecules (donor) and metal oxide nanoparticles (acceptor). Our results yield electron transfer rates of 3.139×108s−1 and 1.182×108s−1 from the GFP molecules to titanium dioxide and cadmium oxide nanoparticles, respectively. The electron transfer rates show a marked decrease with increasing driving force energy. This effect represents a clear example of the Marcus inverted region electron transfer process.
doi_str_mv 10.1016/j.ceramint.2013.10.017
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subjects Cadmium
CdO
D. TiO2
Electron transfer
Fluorescence
GFP
Metal oxides
Nanoparticles
Oxides
Proteins
Titanium dioxide
title Photoinduced electron transfer mechanism between green fluorescent protein molecules and metal oxide nanoparticles
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