True detection limits in an experimental linearly heteroscedastic system

Despite much different processing of the experimental fluorescence detection data presented in Part 1, essentially the same estimates were obtained for the true theoretical Currie decision levels ( Y C and X C ) and true Currie detection limits ( Y D and X D ). The obtained experimental values, for 5% probability of false positives and 5% probability of false negatives, were Y C = 56.0 mV, Y D = 125. mV, X C = 0.132 μg/mL and X D = 0.293 μg/mL. For 5% probability of false positives and 1% probability of false negatives, the obtained detection limits were Y D = 158 . mV and X D = 0.371 μg/mL. Furthermore, by using bootstrapping methodology on the experimental data for the standards and the analytical blank, it was possible to validate previously published experimental domain expressions for the decision levels ( y C and x C ) and detection limits ( y D and x D ). This was demonstrated by testing the generated decision levels and detection limits for their performance in regard to false positives and false negatives. In every case, the obtained numbers of false negatives and false positives were as specified a priori. ► True Currie detection limits are estimated. ► Experimental results validate previous 2008 theory. ► Linearly heteroscedastic system is correctly modeled.