Promoter Activities of Genes cpcT2 and cpcS2 in Nostoc PCC 7120
To study the promoter activities of genes cpcT 2 and cpcS 2,several upstream DNA fragments of these two genes with different lengths were selected.These fragments were fused with promoterless gfp（reporter gene） for constructing five recombinant plasmids.Then,these recombinant plasmids were transferr...
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| Veröffentlicht in: | Wuhan University journal of natural sciences 2012-04, Vol.17 (2), p.169-176 |
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| creator | Chen, Yu Chen, Sili Liu, Li Sun, Yafang Zhou, Ming Zhao, Kaihong Zhang, Juan |
| description | To study the promoter activities of genes cpcT 2 and cpcS 2,several upstream DNA fragments of these two genes with different lengths were selected.These fragments were fused with promoterless gfp（reporter gene） for constructing five recombinant plasmids.Then,these recombinant plasmids were transferred into Nostoc PCC 7120 by conjugation.The promoter activities of genes cpcT 2 and cpcS 2 were determined by observing the fluorescence of green fluorescent protein（GFP） with a fluorescence microscope.The result showed that the 1 300 bp（-1 300 to 0 bp） and 2 600 bp（-2 600 to 0 bp） upstream fragments of cpcT 2 had strong promoter activity and the promoter activity of the 680 bp（-680 to 0 bp） fragment was weaker than that of two above fragments of cpcT 2.The 2 000 bp（-2 000 to 0 bp） upstream fragment of cpcS 2 revealed weak promoter activity.This showed that a strong promoter of cpcT 2 was located in the upstream fragment between-680 and-1 300 bp. |
| doi_str_mv | 10.1007/s11859-012-0823-6 |
| format | Article |
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J. Nat. Sci</addtitle><addtitle>Wuhan University Journal of Natural Sciences</addtitle><description>To study the promoter activities of genes cpcT 2 and cpcS 2,several upstream DNA fragments of these two genes with different lengths were selected.These fragments were fused with promoterless gfp（reporter gene） for constructing five recombinant plasmids.Then,these recombinant plasmids were transferred into Nostoc PCC 7120 by conjugation.The promoter activities of genes cpcT 2 and cpcS 2 were determined by observing the fluorescence of green fluorescent protein（GFP） with a fluorescence microscope.The result showed that the 1 300 bp（-1 300 to 0 bp） and 2 600 bp（-2 600 to 0 bp） upstream fragments of cpcT 2 had strong promoter activity and the promoter activity of the 680 bp（-680 to 0 bp） fragment was weaker than that of two above fragments of cpcT 2.The 2 000 bp（-2 000 to 0 bp） upstream fragment of cpcS 2 revealed weak promoter activity.This showed that a strong promoter of cpcT 2 was located in the upstream fragment between-680 and-1 300 bp.</description><subject>Biomedical and Life Sciences</subject><subject>Computer Science</subject><subject>Conjugation</subject><subject>Fluorescence</subject><subject>Fragments</subject><subject>Genes</subject><subject>Life Sciences</subject><subject>Materials Science</subject><subject>Microscopes</subject><subject>Nostoc</subject><subject>Recombinant</subject><subject>Upstream</subject><issn>1007-1202</issn><issn>1993-4998</issn><fulltext>false</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNqFkD1PwzAQhi0EEqXwA9jMxhK4cxJ_TKiqoCBVUIkyW67jlFRt3NopEv8eV6kYYbp3eN47-yHkGuEOAcR9RJSlygBZBpLlGT8hA1Qqzwql5GnKCcqQATsnFzGuAHJVChyQh1nwG9-5QEe2a76arnGR-ppOXJuC3do5o6atDumd0aalrz523tLZeExF2ndJzmqzju7qOIfk4-lxPn7Opm-Tl_FomllWCJ7VTiKaUpq8ECWonDOsqnKhhONQppfXjC2wEsYWyEsheVVYBxa4BFOlATAkt_3ebfC7vYud3jTRuvXatM7vo0YusFCApfwfhRyZkkqwhGKP2uBjDK7W29BsTPhOkD4o071Xnbzqg1fNU4f1nZjYdumCXvl9aNPn_yzdHA99-na5S73fSwWCQp5U_gCbmYEa</recordid><startdate>201204</startdate><enddate>201204</enddate><creator>Chen, Yu</creator><creator>Chen, Sili</creator><creator>Liu, Li</creator><creator>Sun, Yafang</creator><creator>Zhou, Ming</creator><creator>Zhao, Kaihong</creator><creator>Zhang, Juan</creator><general>Wuhan University</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>~WA</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7SC</scope><scope>7SP</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope></search><sort><creationdate>201204</creationdate><title>Promoter Activities of Genes cpcT2 and cpcS2 in Nostoc PCC 7120</title><author>Chen, Yu ; Chen, Sili ; Liu, Li ; Sun, Yafang ; Zhou, Ming ; Zhao, Kaihong ; Zhang, Juan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2476-fe811a58a3475093621dd5b97e605185f22b1d7ac4165786d4ce0c0680adc0600</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Biomedical and Life Sciences</topic><topic>Computer Science</topic><topic>Conjugation</topic><topic>Fluorescence</topic><topic>Fragments</topic><topic>Genes</topic><topic>Life Sciences</topic><topic>Materials Science</topic><topic>Microscopes</topic><topic>Nostoc</topic><topic>Recombinant</topic><topic>Upstream</topic><toplevel>peer_reviewed</toplevel><creatorcontrib>Chen, Yu</creatorcontrib><creatorcontrib>Chen, Sili</creatorcontrib><creatorcontrib>Liu, Li</creatorcontrib><creatorcontrib>Sun, Yafang</creatorcontrib><creatorcontrib>Zhou, Ming</creatorcontrib><creatorcontrib>Zhao, Kaihong</creatorcontrib><creatorcontrib>Zhang, Juan</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><jtitle>Wuhan University journal of natural sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>no_fulltext</fulltext></delivery><addata><au>Chen, Yu</au><au>Chen, Sili</au><au>Liu, Li</au><au>Sun, Yafang</au><au>Zhou, Ming</au><au>Zhao, Kaihong</au><au>Zhang, Juan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Promoter Activities of Genes cpcT2 and cpcS2 in Nostoc PCC 7120</atitle><jtitle>Wuhan University journal of natural sciences</jtitle><stitle>Wuhan Univ. J. Nat. Sci</stitle><addtitle>Wuhan University Journal of Natural Sciences</addtitle><date>2012-04</date><risdate>2012</risdate><volume>17</volume><issue>2</issue><spage>169</spage><epage>176</epage><pages>169-176</pages><issn>1007-1202</issn><eissn>1993-4998</eissn><abstract>To study the promoter activities of genes cpcT 2 and cpcS 2,several upstream DNA fragments of these two genes with different lengths were selected.These fragments were fused with promoterless gfp（reporter gene） for constructing five recombinant plasmids.Then,these recombinant plasmids were transferred into Nostoc PCC 7120 by conjugation.The promoter activities of genes cpcT 2 and cpcS 2 were determined by observing the fluorescence of green fluorescent protein（GFP） with a fluorescence microscope.The result showed that the 1 300 bp（-1 300 to 0 bp） and 2 600 bp（-2 600 to 0 bp） upstream fragments of cpcT 2 had strong promoter activity and the promoter activity of the 680 bp（-680 to 0 bp） fragment was weaker than that of two above fragments of cpcT 2.The 2 000 bp（-2 000 to 0 bp） upstream fragment of cpcS 2 revealed weak promoter activity.This showed that a strong promoter of cpcT 2 was located in the upstream fragment between-680 and-1 300 bp.</abstract><cop>Heidelberg</cop><pub>Wuhan University</pub><doi>10.1007/s11859-012-0823-6</doi><tpages>8</tpages></addata></record> |
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| issn | 1007-1202 1993-4998 |
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| subjects | Biomedical and Life Sciences Computer Science Conjugation Fluorescence Fragments Genes Life Sciences Materials Science Microscopes Nostoc Recombinant Upstream |
| title | Promoter Activities of Genes cpcT2 and cpcS2 in Nostoc PCC 7120 |
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