Comparative study of three types of polymer materials co-cultured with bone marrow mesenchymal stem cells for use as a myocardial patch in cardiomyocyte regeneration
The purpose of this study was to investigate the most suitable polymer material for supporting stem cell growth as a myocardial patch. After cell isolation and expansion of mouse bone marrow mesenchymal stem cells (BMSC), the cells were induced to differentiate into cardiomyocytes with 5-azacytidine...
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| Veröffentlicht in: | Journal of materials science. Materials in medicine 2013-06, Vol.24 (6), p.1535-1542 |
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| creator | Niu, Hongxing Mu, Junsheng Zhang, Jianqun Hu, Ping Bo, Ping Wang, Yan |
| description | The purpose of this study was to investigate the most suitable polymer material for supporting stem cell growth as a myocardial patch. After cell isolation and expansion of mouse bone marrow mesenchymal stem cells (BMSC), the cells were induced to differentiate into cardiomyocytes with 5-azacytidine to determine their differentiation potential. BMSCs were also seeded onto three types of polymer material film, including polyurethane (PU), 3-hydroxybutyrate-co-4-hydroxybutyrate [P(3HB-co-4HB)], and polypropylene carbonate (PPC). The results revealed that cell numbers were more abundant on both the PU and P(3HB-co-4HB) material surfaces. Conversely, the surface of PPC was smooth with only cell lysate debris observed. The average cell counts were as follows: 143.78 ± 38.38 (PU group), 159.50 ± 33.07 [P(3HB-co-4HB) group], and 1.40 ± 0.70 (PPC group). There was no statistically significant difference in cell numbers between the PU and P(3HB-co-4HB) groups. A statistically significant difference was identified between the PPC group and both the PU (P1) and P(3HB-co-4HB) groups (P2). Polymer biomaterial patches composed of PU and P(3HB-co-4HB) permit good stem cell growth. P(3HB-co-4HB) has the potential for development as a clinical alternative to current treatment methods for the regeneration of cardiomyocytes in patients with myocardial infarction. |
| doi_str_mv | 10.1007/s10856-012-4842-9 |
| format | Article |
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After cell isolation and expansion of mouse bone marrow mesenchymal stem cells (BMSC), the cells were induced to differentiate into cardiomyocytes with 5-azacytidine to determine their differentiation potential. BMSCs were also seeded onto three types of polymer material film, including polyurethane (PU), 3-hydroxybutyrate-co-4-hydroxybutyrate [P(3HB-co-4HB)], and polypropylene carbonate (PPC). The results revealed that cell numbers were more abundant on both the PU and P(3HB-co-4HB) material surfaces. Conversely, the surface of PPC was smooth with only cell lysate debris observed. The average cell counts were as follows: 143.78 ± 38.38 (PU group), 159.50 ± 33.07 [P(3HB-co-4HB) group], and 1.40 ± 0.70 (PPC group). There was no statistically significant difference in cell numbers between the PU and P(3HB-co-4HB) groups. A statistically significant difference was identified between the PPC group and both the PU (P1) and P(3HB-co-4HB) groups (P2). Polymer biomaterial patches composed of PU and P(3HB-co-4HB) permit good stem cell growth. P(3HB-co-4HB) has the potential for development as a clinical alternative to current treatment methods for the regeneration of cardiomyocytes in patients with myocardial infarction.</description><identifier>ISSN: 0957-4530</identifier><identifier>EISSN: 1573-4838</identifier><identifier>DOI: 10.1007/s10856-012-4842-9</identifier><identifier>PMID: 23620011</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Animals ; Biological and medical sciences ; Biomaterials ; Biomedical Engineering and Bioengineering ; Biomedical materials ; Bone marrow ; Cardiomyocytes ; Cell Differentiation ; Cell Proliferation ; Cell Survival ; Cells, Cultured ; Cellular biology ; Ceramics ; Chemistry and Materials Science ; Comparative studies ; Composites ; Equipment Failure Analysis ; Glass ; Guided Tissue Regeneration - instrumentation ; Guided Tissue Regeneration - methods ; Materials Science ; Materials Testing ; Medical sciences ; Mesenchymal Stem Cell Transplantation - instrumentation ; Mesenchymal Stem Cell Transplantation - methods ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - physiology ; Mice ; Mice, Inbred C57BL ; Myocytes, Cardiac - cytology ; Myocytes, Cardiac - physiology ; Natural Materials ; Patients ; Plutonium ; Polymer Sciences ; Polymers ; Polymers - chemical synthesis ; Polypropylenes ; Prosthesis Design ; Regeneration - physiology ; Regenerative Medicine/Tissue Engineering ; Stem cells ; Surfaces and Interfaces ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgical implants ; Technology. Biomaterials. Equipments ; Thin Films ; Tissue Engineering - instrumentation ; Tissue Engineering - methods ; Tissue Scaffolds</subject><ispartof>Journal of materials science. 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Materials in medicine</title><addtitle>J Mater Sci: Mater Med</addtitle><addtitle>J Mater Sci Mater Med</addtitle><description>The purpose of this study was to investigate the most suitable polymer material for supporting stem cell growth as a myocardial patch. After cell isolation and expansion of mouse bone marrow mesenchymal stem cells (BMSC), the cells were induced to differentiate into cardiomyocytes with 5-azacytidine to determine their differentiation potential. BMSCs were also seeded onto three types of polymer material film, including polyurethane (PU), 3-hydroxybutyrate-co-4-hydroxybutyrate [P(3HB-co-4HB)], and polypropylene carbonate (PPC). The results revealed that cell numbers were more abundant on both the PU and P(3HB-co-4HB) material surfaces. Conversely, the surface of PPC was smooth with only cell lysate debris observed. The average cell counts were as follows: 143.78 ± 38.38 (PU group), 159.50 ± 33.07 [P(3HB-co-4HB) group], and 1.40 ± 0.70 (PPC group). There was no statistically significant difference in cell numbers between the PU and P(3HB-co-4HB) groups. A statistically significant difference was identified between the PPC group and both the PU (P1) and P(3HB-co-4HB) groups (P2). Polymer biomaterial patches composed of PU and P(3HB-co-4HB) permit good stem cell growth. P(3HB-co-4HB) has the potential for development as a clinical alternative to current treatment methods for the regeneration of cardiomyocytes in patients with myocardial infarction.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomaterials</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biomedical materials</subject><subject>Bone marrow</subject><subject>Cardiomyocytes</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Cell Survival</subject><subject>Cells, Cultured</subject><subject>Cellular biology</subject><subject>Ceramics</subject><subject>Chemistry and Materials Science</subject><subject>Comparative studies</subject><subject>Composites</subject><subject>Equipment Failure Analysis</subject><subject>Glass</subject><subject>Guided Tissue Regeneration - instrumentation</subject><subject>Guided Tissue Regeneration - methods</subject><subject>Materials Science</subject><subject>Materials Testing</subject><subject>Medical sciences</subject><subject>Mesenchymal Stem Cell Transplantation - instrumentation</subject><subject>Mesenchymal Stem Cell Transplantation - methods</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - physiology</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Myocytes, Cardiac - cytology</subject><subject>Myocytes, Cardiac - physiology</subject><subject>Natural Materials</subject><subject>Patients</subject><subject>Plutonium</subject><subject>Polymer Sciences</subject><subject>Polymers</subject><subject>Polymers - chemical synthesis</subject><subject>Polypropylenes</subject><subject>Prosthesis Design</subject><subject>Regeneration - physiology</subject><subject>Regenerative Medicine/Tissue Engineering</subject><subject>Stem cells</subject><subject>Surfaces and Interfaces</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Surgical implants</subject><subject>Technology. Biomaterials. 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Mu, Junsheng ; Zhang, Jianqun ; Hu, Ping ; Bo, Ping ; Wang, Yan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c505t-4d03c1a19403124a00aef209b8112f8718fe3357a7aa9e4e99bee788c35116b73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomaterials</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Biomedical materials</topic><topic>Bone marrow</topic><topic>Cardiomyocytes</topic><topic>Cell Differentiation</topic><topic>Cell Proliferation</topic><topic>Cell Survival</topic><topic>Cells, Cultured</topic><topic>Cellular biology</topic><topic>Ceramics</topic><topic>Chemistry and Materials Science</topic><topic>Comparative studies</topic><topic>Composites</topic><topic>Equipment Failure Analysis</topic><topic>Glass</topic><topic>Guided Tissue Regeneration - instrumentation</topic><topic>Guided Tissue Regeneration - methods</topic><topic>Materials Science</topic><topic>Materials Testing</topic><topic>Medical sciences</topic><topic>Mesenchymal Stem Cell Transplantation - instrumentation</topic><topic>Mesenchymal Stem Cell Transplantation - methods</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - physiology</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Myocytes, Cardiac - cytology</topic><topic>Myocytes, Cardiac - physiology</topic><topic>Natural Materials</topic><topic>Patients</topic><topic>Plutonium</topic><topic>Polymer Sciences</topic><topic>Polymers</topic><topic>Polymers - chemical synthesis</topic><topic>Polypropylenes</topic><topic>Prosthesis Design</topic><topic>Regeneration - physiology</topic><topic>Regenerative Medicine/Tissue Engineering</topic><topic>Stem cells</topic><topic>Surfaces and Interfaces</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Surgical implants</topic><topic>Technology. Biomaterials. 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Materials in medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Niu, Hongxing</au><au>Mu, Junsheng</au><au>Zhang, Jianqun</au><au>Hu, Ping</au><au>Bo, Ping</au><au>Wang, Yan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative study of three types of polymer materials co-cultured with bone marrow mesenchymal stem cells for use as a myocardial patch in cardiomyocyte regeneration</atitle><jtitle>Journal of materials science. Materials in medicine</jtitle><stitle>J Mater Sci: Mater Med</stitle><addtitle>J Mater Sci Mater Med</addtitle><date>2013-06-01</date><risdate>2013</risdate><volume>24</volume><issue>6</issue><spage>1535</spage><epage>1542</epage><pages>1535-1542</pages><issn>0957-4530</issn><eissn>1573-4838</eissn><abstract>The purpose of this study was to investigate the most suitable polymer material for supporting stem cell growth as a myocardial patch. After cell isolation and expansion of mouse bone marrow mesenchymal stem cells (BMSC), the cells were induced to differentiate into cardiomyocytes with 5-azacytidine to determine their differentiation potential. BMSCs were also seeded onto three types of polymer material film, including polyurethane (PU), 3-hydroxybutyrate-co-4-hydroxybutyrate [P(3HB-co-4HB)], and polypropylene carbonate (PPC). The results revealed that cell numbers were more abundant on both the PU and P(3HB-co-4HB) material surfaces. Conversely, the surface of PPC was smooth with only cell lysate debris observed. The average cell counts were as follows: 143.78 ± 38.38 (PU group), 159.50 ± 33.07 [P(3HB-co-4HB) group], and 1.40 ± 0.70 (PPC group). There was no statistically significant difference in cell numbers between the PU and P(3HB-co-4HB) groups. A statistically significant difference was identified between the PPC group and both the PU (P1) and P(3HB-co-4HB) groups (P2). Polymer biomaterial patches composed of PU and P(3HB-co-4HB) permit good stem cell growth. P(3HB-co-4HB) has the potential for development as a clinical alternative to current treatment methods for the regeneration of cardiomyocytes in patients with myocardial infarction.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>23620011</pmid><doi>10.1007/s10856-012-4842-9</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Biomaterials Biomedical Engineering and Bioengineering Biomedical materials Bone marrow Cardiomyocytes Cell Differentiation Cell Proliferation Cell Survival Cells, Cultured Cellular biology Ceramics Chemistry and Materials Science Comparative studies Composites Equipment Failure Analysis Glass Guided Tissue Regeneration - instrumentation Guided Tissue Regeneration - methods Materials Science Materials Testing Medical sciences Mesenchymal Stem Cell Transplantation - instrumentation Mesenchymal Stem Cell Transplantation - methods Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - physiology Mice Mice, Inbred C57BL Myocytes, Cardiac - cytology Myocytes, Cardiac - physiology Natural Materials Patients Plutonium Polymer Sciences Polymers Polymers - chemical synthesis Polypropylenes Prosthesis Design Regeneration - physiology Regenerative Medicine/Tissue Engineering Stem cells Surfaces and Interfaces Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Surgical implants Technology. Biomaterials. Equipments Thin Films Tissue Engineering - instrumentation Tissue Engineering - methods Tissue Scaffolds |
| title | Comparative study of three types of polymer materials co-cultured with bone marrow mesenchymal stem cells for use as a myocardial patch in cardiomyocyte regeneration |
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