Generation of a cholesterol-independent, non-GS NS0 cell line through chemical treatment and application for high titer antibody production

NS0 cells require exogenous cholesterol for growth. The non-glutamine synthetase (GS) cholesterol-dependent NS0 host was treated with 5-azacytidine (5azaC), a demethylation drug, and adapted to grow in cholesterol-free, chemically defined medium. Within 7 weeks, a stable, cholesterol-independent NS0...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Biotechnology and bioengineering 2012-07, Vol.100 (7), p.1685-1685
Hauptverfasser:	Li, Jincai, Gu, Wei, Edmondson, Diane G, Lu, Connie, Vijayasankaran, Natarajan, Figueroa, Bruno, Stevenson, Dave, Ryll, Thomas, Li, Feng
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies Biotechnology Cell culture Cell growth Cells Chemicals Cholesterol Culture Platforms Productivity Proteins Recombinant
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!