Development of a Bienzymatic Amperometric Glucose Biosensor Using Mesoporous Silica (MCM-41) for Enzyme Immobilization and Its Application on Liquid Pharmaceutical Formulations
This paper describes the development and optimization of an amperometric bienzymatic biosensor for glucose, based on glucose oxidase (GOX) and peroxidase (HRP), immobilized on mesoporous silica with hexagonal symmetry (MCM‐41). The mesoporous material was immobilized by Nafion assisted adsorption on...
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| Veröffentlicht in: | Electroanalysis (New York, N.Y.) N.Y.), 2013-01, Vol.25 (1), p.308-315 |
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| container_title | Electroanalysis (New York, N.Y.) |
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| creator | Caro-Jara, Noemi Mundaca-Uribe, Rodolfo Zaror-Zaror, Claudio Carpinelli-Pavisic, Jorge Aranda-Bustos, Mario Peña-Farfal, Carlos |
| description | This paper describes the development and optimization of an amperometric bienzymatic biosensor for glucose, based on glucose oxidase (GOX) and peroxidase (HRP), immobilized on mesoporous silica with hexagonal symmetry (MCM‐41). The mesoporous material was immobilized by Nafion assisted adsorption on a glassy carbon electrode, which was used as working electrode. Measurements were performed in a conventional three electrode cell using a Ag/AgCl reference electrode and platinum wire as counter electrode. Operating parameters such as GOX/HRP enzyme ratio, mediator (catechol) concentration, applied potential and pH were optimized. The proposed biosensor showed excellent analytical performance with detection and quantification limits of 8.6×10−6 M and 2.5×10−5 M, respectively; analytical sensitivity of 4.67×10−4 AM−1, repeatability |
| doi_str_mv | 10.1002/elan.201200391 |
| format | Article |
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The mesoporous material was immobilized by Nafion assisted adsorption on a glassy carbon electrode, which was used as working electrode. Measurements were performed in a conventional three electrode cell using a Ag/AgCl reference electrode and platinum wire as counter electrode. Operating parameters such as GOX/HRP enzyme ratio, mediator (catechol) concentration, applied potential and pH were optimized. The proposed biosensor showed excellent analytical performance with detection and quantification limits of 8.6×10−6 M and 2.5×10−5 M, respectively; analytical sensitivity of 4.67×10−4 AM−1, repeatability <5 % RSD and a long‐term stability of 10 days (5 daily measurements). Also, five possible interferences were studied and only ascorbic acid showed an amperometric signal, which can be removed with 5 % iodine pretreatment. The biosensor was successfully tested for glucose determination in pharmaceutical formulations, and compared well with an established commercial photometric enzymatic kit (Megazyme).</description><identifier>ISSN: 1040-0397</identifier><identifier>EISSN: 1521-4109</identifier><identifier>DOI: 10.1002/elan.201200391</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Biosensors ; Electrical measurements ; Electrodes ; Enzymes ; Glucose ; Glucose biosensors ; Glucose oxidase ; Mathematical analysis ; Mesoporous silica ; Peroxidase ; Pharmaceuticals ; Silicon dioxide</subject><ispartof>Electroanalysis (New York, N.Y.), 2013-01, Vol.25 (1), p.308-315</ispartof><rights>Copyright © 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3931-4d6025b610404fe7ffd8cd923a07cc9ce86a7f87b4ab7dacf2ebe4b8f4d6dc73</citedby><cites>FETCH-LOGICAL-c3931-4d6025b610404fe7ffd8cd923a07cc9ce86a7f87b4ab7dacf2ebe4b8f4d6dc73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Felan.201200391$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Felan.201200391$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>Caro-Jara, Noemi</creatorcontrib><creatorcontrib>Mundaca-Uribe, Rodolfo</creatorcontrib><creatorcontrib>Zaror-Zaror, Claudio</creatorcontrib><creatorcontrib>Carpinelli-Pavisic, Jorge</creatorcontrib><creatorcontrib>Aranda-Bustos, Mario</creatorcontrib><creatorcontrib>Peña-Farfal, Carlos</creatorcontrib><title>Development of a Bienzymatic Amperometric Glucose Biosensor Using Mesoporous Silica (MCM-41) for Enzyme Immobilization and Its Application on Liquid Pharmaceutical Formulations</title><title>Electroanalysis (New York, N.Y.)</title><addtitle>Electroanalysis</addtitle><description>This paper describes the development and optimization of an amperometric bienzymatic biosensor for glucose, based on glucose oxidase (GOX) and peroxidase (HRP), immobilized on mesoporous silica with hexagonal symmetry (MCM‐41). The mesoporous material was immobilized by Nafion assisted adsorption on a glassy carbon electrode, which was used as working electrode. Measurements were performed in a conventional three electrode cell using a Ag/AgCl reference electrode and platinum wire as counter electrode. Operating parameters such as GOX/HRP enzyme ratio, mediator (catechol) concentration, applied potential and pH were optimized. The proposed biosensor showed excellent analytical performance with detection and quantification limits of 8.6×10−6 M and 2.5×10−5 M, respectively; analytical sensitivity of 4.67×10−4 AM−1, repeatability <5 % RSD and a long‐term stability of 10 days (5 daily measurements). Also, five possible interferences were studied and only ascorbic acid showed an amperometric signal, which can be removed with 5 % iodine pretreatment. The biosensor was successfully tested for glucose determination in pharmaceutical formulations, and compared well with an established commercial photometric enzymatic kit (Megazyme).</description><subject>Biosensors</subject><subject>Electrical measurements</subject><subject>Electrodes</subject><subject>Enzymes</subject><subject>Glucose</subject><subject>Glucose biosensors</subject><subject>Glucose oxidase</subject><subject>Mathematical analysis</subject><subject>Mesoporous silica</subject><subject>Peroxidase</subject><subject>Pharmaceuticals</subject><subject>Silicon dioxide</subject><issn>1040-0397</issn><issn>1521-4109</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqFkc9v0zAUgCMEEmPjytnHcUixYzeuj6XrSrV2Q6JoEhfLcZ7BEMeZnQy6v2p_4pwVTbtNsvzz-579_LLsA8ETgnHxCRrVTgpMCoypIK-yIzItSM4IFq_THDOcp33-NnsX42-MsSiZOMruz-AWGt85aHvkDVLos4X2bu9UbzWauw6Cd9CHtFg1g_YREpD6NvqAvkfb_kRbiL7zwQ8RfbON1QqdbhfbdPFHZBK0HKMBWjvnq3R8lwL7Fqm2Rus-onnXjcrjXmobezPYGn39pYJTGob0CNWgcx_c0DxC8SR7Y1QT4f3_8TjbnS93iy_55mq1Xsw3uaaCprTrEhfTqhzzZga4MfVM16KgCnOthYZZqbiZ8YqpitdKmwIqYNXMJLHWnB5np4ewXfA3A8ReOhs1NOmPIWUqSckJY0XByMsoZYJRLjBN6OSA6uBjDGBkF6xTYS8JlmMR5VhE-VTEJIiD8Nc2sH-BlsvN_PK5mx9cG3v49-Sq8EeWnPKpvL5cSXxGf1yLi1Lu6AMhsrPP</recordid><startdate>201301</startdate><enddate>201301</enddate><creator>Caro-Jara, Noemi</creator><creator>Mundaca-Uribe, Rodolfo</creator><creator>Zaror-Zaror, Claudio</creator><creator>Carpinelli-Pavisic, Jorge</creator><creator>Aranda-Bustos, Mario</creator><creator>Peña-Farfal, Carlos</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7U5</scope><scope>L7M</scope></search><sort><creationdate>201301</creationdate><title>Development of a Bienzymatic Amperometric Glucose Biosensor Using Mesoporous Silica (MCM-41) for Enzyme Immobilization and Its Application on Liquid Pharmaceutical Formulations</title><author>Caro-Jara, Noemi ; Mundaca-Uribe, Rodolfo ; Zaror-Zaror, Claudio ; Carpinelli-Pavisic, Jorge ; Aranda-Bustos, Mario ; Peña-Farfal, Carlos</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3931-4d6025b610404fe7ffd8cd923a07cc9ce86a7f87b4ab7dacf2ebe4b8f4d6dc73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Biosensors</topic><topic>Electrical measurements</topic><topic>Electrodes</topic><topic>Enzymes</topic><topic>Glucose</topic><topic>Glucose biosensors</topic><topic>Glucose oxidase</topic><topic>Mathematical analysis</topic><topic>Mesoporous silica</topic><topic>Peroxidase</topic><topic>Pharmaceuticals</topic><topic>Silicon dioxide</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Caro-Jara, Noemi</creatorcontrib><creatorcontrib>Mundaca-Uribe, Rodolfo</creatorcontrib><creatorcontrib>Zaror-Zaror, Claudio</creatorcontrib><creatorcontrib>Carpinelli-Pavisic, Jorge</creatorcontrib><creatorcontrib>Aranda-Bustos, Mario</creatorcontrib><creatorcontrib>Peña-Farfal, Carlos</creatorcontrib><collection>Istex</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Electroanalysis (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Caro-Jara, Noemi</au><au>Mundaca-Uribe, Rodolfo</au><au>Zaror-Zaror, Claudio</au><au>Carpinelli-Pavisic, Jorge</au><au>Aranda-Bustos, Mario</au><au>Peña-Farfal, Carlos</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a Bienzymatic Amperometric Glucose Biosensor Using Mesoporous Silica (MCM-41) for Enzyme Immobilization and Its Application on Liquid Pharmaceutical Formulations</atitle><jtitle>Electroanalysis (New York, N.Y.)</jtitle><addtitle>Electroanalysis</addtitle><date>2013-01</date><risdate>2013</risdate><volume>25</volume><issue>1</issue><spage>308</spage><epage>315</epage><pages>308-315</pages><issn>1040-0397</issn><eissn>1521-4109</eissn><abstract>This paper describes the development and optimization of an amperometric bienzymatic biosensor for glucose, based on glucose oxidase (GOX) and peroxidase (HRP), immobilized on mesoporous silica with hexagonal symmetry (MCM‐41). The mesoporous material was immobilized by Nafion assisted adsorption on a glassy carbon electrode, which was used as working electrode. Measurements were performed in a conventional three electrode cell using a Ag/AgCl reference electrode and platinum wire as counter electrode. Operating parameters such as GOX/HRP enzyme ratio, mediator (catechol) concentration, applied potential and pH were optimized. The proposed biosensor showed excellent analytical performance with detection and quantification limits of 8.6×10−6 M and 2.5×10−5 M, respectively; analytical sensitivity of 4.67×10−4 AM−1, repeatability <5 % RSD and a long‐term stability of 10 days (5 daily measurements). Also, five possible interferences were studied and only ascorbic acid showed an amperometric signal, which can be removed with 5 % iodine pretreatment. 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| subjects | Biosensors Electrical measurements Electrodes Enzymes Glucose Glucose biosensors Glucose oxidase Mathematical analysis Mesoporous silica Peroxidase Pharmaceuticals Silicon dioxide |
| title | Development of a Bienzymatic Amperometric Glucose Biosensor Using Mesoporous Silica (MCM-41) for Enzyme Immobilization and Its Application on Liquid Pharmaceutical Formulations |
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