High-level secretory production of recombinant single-chain variable fragment (scFv) in Corynebacterium glutamicum

We describe the development of a new secretory production system for the enhanced production of a single-chain variable fragment (scFv) against the anthrax toxin in Corynebacterium glutamicum . For efficient secretory production of the antibody fragment, the following components were examined: (1) s...

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Veröffentlicht in:	Applied microbiology and biotechnology 2014-01, Vol.98 (1), p.273-284
Hauptverfasser:	Yim, Sung Sun, An, Seul Ji, Choi, Jae Woong, Ryu, Ae Jin, Jeong, Ki Jun
Format:	Artikel
Sprache:	eng
Schlagworte:	Actinomycetes Anthrax Antibodies Antibodies, Bacterial - genetics Antibodies, Bacterial - metabolism Antigens, Bacterial - immunology Applied Genetics and Molecular Biotechnology Bacterial Toxins - immunology Biomedical and Life Sciences Bioreactors Biotechnology Corynebacterium glutamicum Corynebacterium glutamicum - genetics Corynebacterium glutamicum - metabolism Cultivation Culture E coli Engineering Enzyme-linked immunosorbent assay Enzymes Fragmentation Gene expression Genetic engineering Immunohistochemistry Life Sciences Microbial Genetics and Genomics Microbiology Molecular Sequence Data Monoclonal antibodies Peptides Pharmaceutical sciences Pharmaceuticals Physiological aspects Plasmids Proteins Recombinant Recombinant Proteins - genetics Recombinant Proteins - metabolism Sequence Analysis, DNA Single-Chain Antibodies - genetics Single-Chain Antibodies - metabolism Studies Toxins
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