Polymer-based alternative method to extract bromelain from pineapple peel waste
Bromelain is a mixture of proteolytic enzymes present in all tissues of the pineapple (Ananas comosus Merr.), and it is known for its clinical therapeutic applications, food processing, and as a dietary supplement. The use of pineapple waste for bromelain extraction is interesting from both an envir...
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| Veröffentlicht in: | Biotechnology and applied biochemistry 2013-09, Vol.60 (5), p.527-535 |
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| container_title | Biotechnology and applied biochemistry |
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| creator | de Lencastre Novaes, Letícia Celia de Carvalho Santos Ebinuma, Valéria Mazzola, Priscila Gava Júnior, Adalberto Pessoa |
| description | Bromelain is a mixture of proteolytic enzymes present in all tissues of the pineapple (Ananas comosus Merr.), and it is known for its clinical therapeutic applications, food processing, and as a dietary supplement. The use of pineapple waste for bromelain extraction is interesting from both an environmental and a commercial point of view, because the protease has relevant clinical potential. We aimed to study the optimization of bromelain extraction from pineapple waste, using the aqueous two‐phase system formed by polyethylene glycol (PEG) and poly(acrylic acid). In this work, bromelain partitioned preferentially to the top/PEG‐rich phase and, in the best condition, achieved a yield of 335.27% with a purification factor of 25.78. The statistical analysis showed that all variables analyzed were significant to the process. |
| doi_str_mv | 10.1002/bab.1121 |
| format | Article |
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The use of pineapple waste for bromelain extraction is interesting from both an environmental and a commercial point of view, because the protease has relevant clinical potential. We aimed to study the optimization of bromelain extraction from pineapple waste, using the aqueous two‐phase system formed by polyethylene glycol (PEG) and poly(acrylic acid). In this work, bromelain partitioned preferentially to the top/PEG‐rich phase and, in the best condition, achieved a yield of 335.27% with a purification factor of 25.78. The statistical analysis showed that all variables analyzed were significant to the process.</description><identifier>ISSN: 0885-4513</identifier><identifier>EISSN: 1470-8744</identifier><identifier>DOI: 10.1002/bab.1121</identifier><identifier>PMID: 24011234</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Acrylic Resins - chemistry ; Ananas - enzymology ; Ananas comosus ; aqueous two-phase system ; Biochemistry ; Biotechnology ; bromelain ; Bromelains - chemistry ; Bromelains - isolation & purification ; Dietary supplements ; Extraction ; Industrial Waste ; Optimization ; pineapple waste ; Pineapples ; poly(acrylic acid) ; Polyethylene glycol ; Polyethylene Glycols - chemistry ; Polymers ; Purification ; Refuse Disposal ; Wastes</subject><ispartof>Biotechnology and applied biochemistry, 2013-09, Vol.60 (5), p.527-535</ispartof><rights>2013 International Union of Biochemistry and Molecular Biology, Inc.</rights><rights>Copyright © 2013 International Union of Biochemistry and Molecular Biology, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4871-76501319741080e6b5698b4f3559573592b27b1a078436a1fa3396e174dabd423</citedby><cites>FETCH-LOGICAL-c4871-76501319741080e6b5698b4f3559573592b27b1a078436a1fa3396e174dabd423</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbab.1121$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbab.1121$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24011234$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Lencastre Novaes, Letícia Celia</creatorcontrib><creatorcontrib>de Carvalho Santos Ebinuma, Valéria</creatorcontrib><creatorcontrib>Mazzola, Priscila Gava</creatorcontrib><creatorcontrib>Júnior, Adalberto Pessoa</creatorcontrib><title>Polymer-based alternative method to extract bromelain from pineapple peel waste</title><title>Biotechnology and applied biochemistry</title><addtitle>Biotechnology and Applied Biochemistry</addtitle><description>Bromelain is a mixture of proteolytic enzymes present in all tissues of the pineapple (Ananas comosus Merr.), and it is known for its clinical therapeutic applications, food processing, and as a dietary supplement. 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The statistical analysis showed that all variables analyzed were significant to the process.</description><subject>Acrylic Resins - chemistry</subject><subject>Ananas - enzymology</subject><subject>Ananas comosus</subject><subject>aqueous two-phase system</subject><subject>Biochemistry</subject><subject>Biotechnology</subject><subject>bromelain</subject><subject>Bromelains - chemistry</subject><subject>Bromelains - isolation & purification</subject><subject>Dietary supplements</subject><subject>Extraction</subject><subject>Industrial Waste</subject><subject>Optimization</subject><subject>pineapple waste</subject><subject>Pineapples</subject><subject>poly(acrylic acid)</subject><subject>Polyethylene glycol</subject><subject>Polyethylene Glycols - chemistry</subject><subject>Polymers</subject><subject>Purification</subject><subject>Refuse Disposal</subject><subject>Wastes</subject><issn>0885-4513</issn><issn>1470-8744</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0U9rFDEYx_EgFrtWoa9AAl68TJtn8uTPHNvFrkJpS1H0FpKdZ3BqZmdMZm333Ttrty0Ioqfk8OF7eH6MHYI4AiHK4-DDEUAJz9gM0IjCGsTnbCasVQUqkPvsZc43QghrbPmC7ZcoJi5xxi6v-rjpKBXBZ6q5jyOllR_bn8Q7Gr_1NR97Tndj8suRh9R3FH274s3040O7Ij8MkfhAFPmtzyO9YnuNj5le794D9vns_af5h-L8cvFxfnJeLNEaKIxWAiRUBkFYQTooXdmAjVSqUkaqqgylCeCFsSi1h8ZLWWkCg7UPNZbygL277w6p_7GmPLquzUuK0a-oX2cH2gBOt7Di3xQ1KmEqXf0HRQOiKmFL3_5Bb_r1dLr4W6FW2mj7FFymPudEjRtS2_m0cSDcdjo3Tee20030zS64Dh3Vj_BhqwkU9-C2jbT5a8idnpzugjvfTrvcPXqfvjttpFHuy8XCqfnZ4hq_ogP5C2sRrQQ</recordid><startdate>201309</startdate><enddate>201309</enddate><creator>de Lencastre Novaes, Letícia Celia</creator><creator>de Carvalho Santos Ebinuma, Valéria</creator><creator>Mazzola, Priscila Gava</creator><creator>Júnior, Adalberto Pessoa</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TB</scope><scope>7TK</scope><scope>7U5</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>L7M</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>7SR</scope><scope>JG9</scope></search><sort><creationdate>201309</creationdate><title>Polymer-based alternative method to extract bromelain from pineapple peel waste</title><author>de Lencastre Novaes, Letícia Celia ; 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| subjects | Acrylic Resins - chemistry Ananas - enzymology Ananas comosus aqueous two-phase system Biochemistry Biotechnology bromelain Bromelains - chemistry Bromelains - isolation & purification Dietary supplements Extraction Industrial Waste Optimization pineapple waste Pineapples poly(acrylic acid) Polyethylene glycol Polyethylene Glycols - chemistry Polymers Purification Refuse Disposal Wastes |
| title | Polymer-based alternative method to extract bromelain from pineapple peel waste |
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