Hydrogen sulfide induced by nitric oxide mediates ethylene-induced stomatal closure of Arabidopsis thaliana
Pharmacological, laser scanning confocal microscopic （LSCM）, real-time PCR and spectrophotographic approaches are used to study the roles of hydrogen sulfide （H2S） and nitric oxide （NO） in signaling transduction of stomatal movement response to ethylene in Arabidopsis thaliana. In the present study,...
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| Veröffentlicht in: | Chinese science bulletin 2011-11, Vol.56 (33), p.3547-3553 |
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| creator | Liu, Jing Hou, LiXia Liu, GuoHua Liu, Xin Wang, XueChen |
| description | Pharmacological, laser scanning confocal microscopic （LSCM）, real-time PCR and spectrophotographic approaches are used to study the roles of hydrogen sulfide （H2S） and nitric oxide （NO） in signaling transduction of stomatal movement response to ethylene in Arabidopsis thaliana. In the present study, inhibitors of H2S synthesis were found to block ethylene-induced stomatal closure of Arabidopsis. Treatment with ethylene induced H2S generation and increased L-/D-cysteine desulfhydrase （pyridoxal- phosphate-dependent enzyme） activity in leaves. Quantitative PCR analysis showed AtL-CDes and AtD-CDes transcripts were induced by ethylene. It is suggested that ethylene-induced H2S levels and L-/D-cysteine desulfhydrase activity decreased when NO was compromised. The data clearly show that ethylene was able to induce H2S generation and stomatal closure in Atnoal plants, but failed in the Atnial,nia2 mutant. Inhibitors of H2S synthesis had no effect on ethylene-induced NO accumulation and nitrate reductase （NR） activity in guard cells or leaves of Arabidopsis, whereas ethylene was able to induce NO synthesis. There- fore, we conclude that H2S and NO are involved in the signal transduction pathway of ethylene-induced stomatal closure. In Arabidopsis, H2S may represent a novel downstream indicator of NO during ethylene-induced stomatal movement. |
| doi_str_mv | 10.1007/s11434-011-4819-y |
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In the present study, inhibitors of H2S synthesis were found to block ethylene-induced stomatal closure of Arabidopsis. Treatment with ethylene induced H2S generation and increased L-/D-cysteine desulfhydrase （pyridoxal- phosphate-dependent enzyme） activity in leaves. Quantitative PCR analysis showed AtL-CDes and AtD-CDes transcripts were induced by ethylene. It is suggested that ethylene-induced H2S levels and L-/D-cysteine desulfhydrase activity decreased when NO was compromised. The data clearly show that ethylene was able to induce H2S generation and stomatal closure in Atnoal plants, but failed in the Atnial,nia2 mutant. Inhibitors of H2S synthesis had no effect on ethylene-induced NO accumulation and nitrate reductase （NR） activity in guard cells or leaves of Arabidopsis, whereas ethylene was able to induce NO synthesis. There- fore, we conclude that H2S and NO are involved in the signal transduction pathway of ethylene-induced stomatal closure. In Arabidopsis, H2S may represent a novel downstream indicator of NO during ethylene-induced stomatal movement.</description><identifier>ISSN: 1001-6538</identifier><identifier>EISSN: 1861-9541</identifier><identifier>DOI: 10.1007/s11434-011-4819-y</identifier><language>eng</language><publisher>Heidelberg: Springer-Verlag</publisher><subject>Arabidopsis thaliana ; Chemistry/Food Science ; Earth Sciences ; Engineering ; Enzymes ; Ethylene ; guard cells ; Humanities and Social Sciences ; Hydrogen sulfide ; Inhibitors ; Leaves ; Life Sciences ; Movement ; multidisciplinary ; nitrate reductase ; Nitric oxide ; Physics ; quantitative polymerase chain reaction ; Science ; Science (multidisciplinary) ; signal transduction ; stomatal movement ; Synthesis ; 一氧化氮 ; 乙烯 ; 信号转导通路 ; 拟南芥 ; 气孔关闭 ; 激光扫描共聚焦显微镜 ; 硫化氢 ; 诱导</subject><ispartof>Chinese science bulletin, 2011-11, Vol.56 (33), p.3547-3553</ispartof><rights>The Author(s) 2011. This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution and reproduction in any medium, provided the original author(s) and source are credited.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c414t-9d9605dd2125a6f19cde95794d80d05ee175e71946036ff32671c086acfd70e83</citedby><cites>FETCH-LOGICAL-c414t-9d9605dd2125a6f19cde95794d80d05ee175e71946036ff32671c086acfd70e83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/86894X/86894X.jpg</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids></links><search><creatorcontrib>Liu, Jing</creatorcontrib><creatorcontrib>Hou, LiXia</creatorcontrib><creatorcontrib>Liu, GuoHua</creatorcontrib><creatorcontrib>Liu, Xin</creatorcontrib><creatorcontrib>Wang, XueChen</creatorcontrib><title>Hydrogen sulfide induced by nitric oxide mediates ethylene-induced stomatal closure of Arabidopsis thaliana</title><title>Chinese science bulletin</title><addtitle>Chin. Sci. Bull</addtitle><addtitle>Chinese Science Bulletin</addtitle><description>Pharmacological, laser scanning confocal microscopic （LSCM）, real-time PCR and spectrophotographic approaches are used to study the roles of hydrogen sulfide （H2S） and nitric oxide （NO） in signaling transduction of stomatal movement response to ethylene in Arabidopsis thaliana. In the present study, inhibitors of H2S synthesis were found to block ethylene-induced stomatal closure of Arabidopsis. Treatment with ethylene induced H2S generation and increased L-/D-cysteine desulfhydrase （pyridoxal- phosphate-dependent enzyme） activity in leaves. Quantitative PCR analysis showed AtL-CDes and AtD-CDes transcripts were induced by ethylene. It is suggested that ethylene-induced H2S levels and L-/D-cysteine desulfhydrase activity decreased when NO was compromised. The data clearly show that ethylene was able to induce H2S generation and stomatal closure in Atnoal plants, but failed in the Atnial,nia2 mutant. Inhibitors of H2S synthesis had no effect on ethylene-induced NO accumulation and nitrate reductase （NR） activity in guard cells or leaves of Arabidopsis, whereas ethylene was able to induce NO synthesis. There- fore, we conclude that H2S and NO are involved in the signal transduction pathway of ethylene-induced stomatal closure. In Arabidopsis, H2S may represent a novel downstream indicator of NO during ethylene-induced stomatal movement.</description><subject>Arabidopsis thaliana</subject><subject>Chemistry/Food Science</subject><subject>Earth Sciences</subject><subject>Engineering</subject><subject>Enzymes</subject><subject>Ethylene</subject><subject>guard cells</subject><subject>Humanities and Social Sciences</subject><subject>Hydrogen sulfide</subject><subject>Inhibitors</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Movement</subject><subject>multidisciplinary</subject><subject>nitrate reductase</subject><subject>Nitric oxide</subject><subject>Physics</subject><subject>quantitative polymerase chain reaction</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>signal transduction</subject><subject>stomatal movement</subject><subject>Synthesis</subject><subject>一氧化氮</subject><subject>乙烯</subject><subject>信号转导通路</subject><subject>拟南芥</subject><subject>气孔关闭</subject><subject>激光扫描共聚焦显微镜</subject><subject>硫化氢</subject><subject>诱导</subject><issn>1001-6538</issn><issn>1861-9541</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><recordid>eNp9kc1u1DAURiNEpZbSB-gKs2NjuDd24nhZVUCRKrGAri03vp5xydhTO5HI2-NRCktW_tE5V58_N801wkcEUJ8KohSSAyKXA2q-vmoucOiR607i67oHQN53Yjhv3pTyVE8CVXvR_LpbXU47iqwskw-OWIhuGcmxx5XFMOcwsvT7dH8gF-xMhdG8XyeKxP-SZU4HO9uJjVMqSyaWPLvJ9jG4dCyhsHlvp2CjfduceTsVunpZL5uHL59_3t7x--9fv93e3PNRopy5drqHzrkW2872HvXoSHdKSzeAg44IVUcKtexB9N6Ltlc4wtDb0TsFNIjL5sM295jT80JlNodQRpomGyktxWAVJCgcoKK4oWNOpWTy5pjDwebVIJhTsWYr1tRizalYs1an3ZxS2bijbJ7SkmN90X-ld5vkbTJ2l0MxDz9aqDkAQSnRVeL9S5R9irvnOvlfFqHrT4KS4g8fspF9</recordid><startdate>20111101</startdate><enddate>20111101</enddate><creator>Liu, Jing</creator><creator>Hou, LiXia</creator><creator>Liu, GuoHua</creator><creator>Liu, Xin</creator><creator>Wang, XueChen</creator><general>Springer-Verlag</general><general>SP Science China Press</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>~WA</scope><scope>FBQ</scope><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SC</scope><scope>7SP</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope></search><sort><creationdate>20111101</creationdate><title>Hydrogen sulfide induced by nitric oxide mediates ethylene-induced stomatal closure of Arabidopsis thaliana</title><author>Liu, Jing ; Hou, LiXia ; Liu, GuoHua ; Liu, Xin ; Wang, XueChen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-9d9605dd2125a6f19cde95794d80d05ee175e71946036ff32671c086acfd70e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Arabidopsis thaliana</topic><topic>Chemistry/Food Science</topic><topic>Earth Sciences</topic><topic>Engineering</topic><topic>Enzymes</topic><topic>Ethylene</topic><topic>guard cells</topic><topic>Humanities and Social Sciences</topic><topic>Hydrogen sulfide</topic><topic>Inhibitors</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Movement</topic><topic>multidisciplinary</topic><topic>nitrate reductase</topic><topic>Nitric oxide</topic><topic>Physics</topic><topic>quantitative polymerase chain reaction</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>signal transduction</topic><topic>stomatal movement</topic><topic>Synthesis</topic><topic>一氧化氮</topic><topic>乙烯</topic><topic>信号转导通路</topic><topic>拟南芥</topic><topic>气孔关闭</topic><topic>激光扫描共聚焦显微镜</topic><topic>硫化氢</topic><topic>诱导</topic><toplevel>online_resources</toplevel><creatorcontrib>Liu, Jing</creatorcontrib><creatorcontrib>Hou, LiXia</creatorcontrib><creatorcontrib>Liu, GuoHua</creatorcontrib><creatorcontrib>Liu, Xin</creatorcontrib><creatorcontrib>Wang, XueChen</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>AGRIS</collection><collection>Springer Nature OA Free Journals</collection><collection>CrossRef</collection><collection>Computer and Information Systems Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><jtitle>Chinese science bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Jing</au><au>Hou, LiXia</au><au>Liu, GuoHua</au><au>Liu, Xin</au><au>Wang, XueChen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hydrogen sulfide induced by nitric oxide mediates ethylene-induced stomatal closure of Arabidopsis thaliana</atitle><jtitle>Chinese science bulletin</jtitle><stitle>Chin. 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It is suggested that ethylene-induced H2S levels and L-/D-cysteine desulfhydrase activity decreased when NO was compromised. The data clearly show that ethylene was able to induce H2S generation and stomatal closure in Atnoal plants, but failed in the Atnial,nia2 mutant. Inhibitors of H2S synthesis had no effect on ethylene-induced NO accumulation and nitrate reductase （NR） activity in guard cells or leaves of Arabidopsis, whereas ethylene was able to induce NO synthesis. There- fore, we conclude that H2S and NO are involved in the signal transduction pathway of ethylene-induced stomatal closure. In Arabidopsis, H2S may represent a novel downstream indicator of NO during ethylene-induced stomatal movement.</abstract><cop>Heidelberg</cop><pub>Springer-Verlag</pub><doi>10.1007/s11434-011-4819-y</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Arabidopsis thaliana Chemistry/Food Science Earth Sciences Engineering Enzymes Ethylene guard cells Humanities and Social Sciences Hydrogen sulfide Inhibitors Leaves Life Sciences Movement multidisciplinary nitrate reductase Nitric oxide Physics quantitative polymerase chain reaction Science Science (multidisciplinary) signal transduction stomatal movement Synthesis 一氧化氮 乙烯 信号转导通路 拟南芥 气孔关闭 激光扫描共聚焦显微镜 硫化氢 诱导 |
| title | Hydrogen sulfide induced by nitric oxide mediates ethylene-induced stomatal closure of Arabidopsis thaliana |
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