Reduction of Artifacts in Fluorescence Correlation Spectroscopy Due to Sample Adsorption on Optical Glass Surfaces

The preparation of glass cell surfaces that are chemically functionalized with poly(ethylene glycol) (PEG) chains to reduce sample adsorption and their use in fluorescence correlation spectroscopy (FCS) is described. Optical glass coverslips were acid etched and reacted with either 750 Mr PEG (PEG-7...

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Veröffentlicht in:	Applied spectroscopy 2013-06, Vol.67 (6), p.692-698
Hauptverfasser:	Turner, Daniel K., Wayman, Ashley E., Rolando, Chelsey N., Dande, Prasad, Carter, Phillip W., Remsen, Edward E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adsorption Animals Cattle Correlation Fluorescence Glass Glass - chemistry Octoxynol - chemistry Optical glass Polyethylene Glycols - chemistry Rhodamines - analysis Rhodamines - chemistry Rhodamines - metabolism Serum Albumin, Bovine - analysis Serum Albumin, Bovine - chemistry Serum Albumin, Bovine - metabolism Spectrometry, Fluorescence - methods Spectroscopy Surface chemistry
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