Development of an analytical method for yam saponins using HPLC with pulsed amperometric detection at different column temperatures

Yam saponins (dioscin, gracillin, protodioscin, and protogracillin) were analyzed with three different C18 columns at incremental column temperatures from 15 to 45°C to investigate the effect of temperature on the retention and resolution of yam saponins. At low temperature, yam saponins showed decr...

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Veröffentlicht in:	Journal of separation science 2013-02, Vol.36 (4), p.690-698
Hauptverfasser:	Kwon, Ha-Jeong, Choi, Seung-Hee, Yoo, Chang-Seon, Choi, Hwa-Young, Lee, Soo-Eon, Park, Yong-Duk
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Biological and medical sciences Chromatography Chromatography, High Pressure Liquid - instrumentation Chromatography, High Pressure Liquid - methods Correlation coefficients Dioscorea - chemistry Electrical measurements Food industries Food science Foods Fruit and vegetable industries Fundamental and applied biological sciences. Psychology General pharmacology Mathematical analysis Medical sciences Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Plant Extracts - analysis Pulsed amperometric detection Purification Resolution Retention time Saponins Saponins - analysis Separation Temperature effect Yam saponin
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