Acceleration of neuronal precursors differentiation induced by substrate nanotopography
Embryonic stem (ES) cell differentiation in specific cell lineages is a major issue in cell biology particularly in regenerative medicine. Differentiation is usually achieved by using biochemical factors and it is not clear whether mechanical properties of the substrate over which cells are grown ca...
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| Veröffentlicht in: | Biotechnology and bioengineering 2011-11, Vol.108 (11), p.2736-2746 |
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| container_title | Biotechnology and bioengineering |
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| creator | Migliorini, Elisa Grenci, Gianluca Ban, Jelena Pozzato, Alessandro Tormen, Massimo Lazzarino, Marco Torre, Vincent Ruaro, Maria Elisabetta |
| description | Embryonic stem (ES) cell differentiation in specific cell lineages is a major issue in cell biology particularly in regenerative medicine. Differentiation is usually achieved by using biochemical factors and it is not clear whether mechanical properties of the substrate over which cells are grown can affect proliferation and differentiation. Therefore, we produced patterns in polydimethylsiloxane (PDMS) consisting of groove and pillar arrays of sub‐micrometric lateral resolution as substrates for cell cultures. We analyzed the effect of different nanostructures on differentiation of ES‐derived neuronal precursors into neuronal lineage without adding biochemical factors. Neuronal precursors adhered on PDMS more effectively than on glass coverslips. We demonstrated that neuronal yield was enhanced by increasing pillars height from 35 to 400 nm. On higher pillar neuronal differentiation reaches ∼80% 96 h after plating and the largest differentiation enhancement of pillars over flat PDMS was observed during the first 6 h of culture. We conclude that PDMS nanopillars accelerate and increase neuronal differentiation. Biotechnol. Bioeng. 2011;108: 2736–2746. © 2011 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/bit.23232 |
| format | Article |
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Differentiation is usually achieved by using biochemical factors and it is not clear whether mechanical properties of the substrate over which cells are grown can affect proliferation and differentiation. Therefore, we produced patterns in polydimethylsiloxane (PDMS) consisting of groove and pillar arrays of sub‐micrometric lateral resolution as substrates for cell cultures. We analyzed the effect of different nanostructures on differentiation of ES‐derived neuronal precursors into neuronal lineage without adding biochemical factors. Neuronal precursors adhered on PDMS more effectively than on glass coverslips. We demonstrated that neuronal yield was enhanced by increasing pillars height from 35 to 400 nm. On higher pillar neuronal differentiation reaches ∼80% 96 h after plating and the largest differentiation enhancement of pillars over flat PDMS was observed during the first 6 h of culture. We conclude that PDMS nanopillars accelerate and increase neuronal differentiation. Biotechnol. Bioeng. 2011;108: 2736–2746. © 2011 Wiley Periodicals, Inc.</description><identifier>ISSN: 0006-3592</identifier><identifier>ISSN: 1097-0290</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.23232</identifier><identifier>PMID: 21656711</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Biochemistry ; Biological and medical sciences ; Biotechnology ; Cell Culture Techniques - methods ; Cell Differentiation ; Cell growth ; Cell Proliferation ; Culture ; Differentiation ; Dimethylpolysiloxanes ; Diverse techniques ; embryonic stem cells ; Embryonic Stem Cells - physiology ; Fundamental and applied biological sciences. Psychology ; Health. Pharmaceutical industry ; Industrial applications and implications. Economical aspects ; Mice ; Miscellaneous ; Molecular and cellular biology ; Nanocomposites ; Nanomaterials ; Nanostructure ; Nanostructures ; nanotopography ; neuronal differentiation ; Neurons ; Neurons - physiology ; pillar ; Pillars ; Polydimethylsiloxane ; Precursors ; Silicone resins ; Stem cells ; Surface Properties</subject><ispartof>Biotechnology and bioengineering, 2011-11, Vol.108 (11), p.2736-2746</ispartof><rights>Copyright © 2011 Wiley Periodicals, Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright John Wiley and Sons, Limited Nov 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5512-3a345a5d11e433c7d14fcc9f50e0beeed629b921b2973cad29d7c1c64aa17a83</citedby><cites>FETCH-LOGICAL-c5512-3a345a5d11e433c7d14fcc9f50e0beeed629b921b2973cad29d7c1c64aa17a83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.23232$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.23232$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24599013$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21656711$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Migliorini, Elisa</creatorcontrib><creatorcontrib>Grenci, Gianluca</creatorcontrib><creatorcontrib>Ban, Jelena</creatorcontrib><creatorcontrib>Pozzato, Alessandro</creatorcontrib><creatorcontrib>Tormen, Massimo</creatorcontrib><creatorcontrib>Lazzarino, Marco</creatorcontrib><creatorcontrib>Torre, Vincent</creatorcontrib><creatorcontrib>Ruaro, Maria Elisabetta</creatorcontrib><title>Acceleration of neuronal precursors differentiation induced by substrate nanotopography</title><title>Biotechnology and bioengineering</title><addtitle>Biotechnol. Bioeng</addtitle><description>Embryonic stem (ES) cell differentiation in specific cell lineages is a major issue in cell biology particularly in regenerative medicine. Differentiation is usually achieved by using biochemical factors and it is not clear whether mechanical properties of the substrate over which cells are grown can affect proliferation and differentiation. Therefore, we produced patterns in polydimethylsiloxane (PDMS) consisting of groove and pillar arrays of sub‐micrometric lateral resolution as substrates for cell cultures. We analyzed the effect of different nanostructures on differentiation of ES‐derived neuronal precursors into neuronal lineage without adding biochemical factors. Neuronal precursors adhered on PDMS more effectively than on glass coverslips. We demonstrated that neuronal yield was enhanced by increasing pillars height from 35 to 400 nm. On higher pillar neuronal differentiation reaches ∼80% 96 h after plating and the largest differentiation enhancement of pillars over flat PDMS was observed during the first 6 h of culture. We conclude that PDMS nanopillars accelerate and increase neuronal differentiation. Biotechnol. Bioeng. 2011;108: 2736–2746. © 2011 Wiley Periodicals, Inc.</description><subject>Animals</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Differentiation</subject><subject>Cell growth</subject><subject>Cell Proliferation</subject><subject>Culture</subject><subject>Differentiation</subject><subject>Dimethylpolysiloxanes</subject><subject>Diverse techniques</subject><subject>embryonic stem cells</subject><subject>Embryonic Stem Cells - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Health. 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Economical aspects</subject><subject>Mice</subject><subject>Miscellaneous</subject><subject>Molecular and cellular biology</subject><subject>Nanocomposites</subject><subject>Nanomaterials</subject><subject>Nanostructure</subject><subject>Nanostructures</subject><subject>nanotopography</subject><subject>neuronal differentiation</subject><subject>Neurons</subject><subject>Neurons - physiology</subject><subject>pillar</subject><subject>Pillars</subject><subject>Polydimethylsiloxane</subject><subject>Precursors</subject><subject>Silicone resins</subject><subject>Stem cells</subject><subject>Surface Properties</subject><issn>0006-3592</issn><issn>1097-0290</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV9rFDEUxYModl198AvIIAj1Ydr8mSSTx7a0a6HUBxf6GDLJHU2dTcZkBt1vb3S2FQSRBMKF3zmXk4PQa4JPCMb0tPPTCWXlPEErgpWsMVX4KVphjEXNuKJH6EXO92WUrRDP0RElggtJyArdnVkLAyQz-Riq2FcB5hSDGaoxgZ1TjilXzvc9JAiTXzAf3GzBVd2-ynOXp6KGKpgQpzjGz8mMX_Yv0bPeDBleHd412l5dbi8-1DcfN9cXZze15ZzQmhnWcMMdIdAwZqUjTW-t6jkG3AGAE1R1ipKOKsmscVQ5aYkVjTFEmpat0fFiO6b4bYY86Z3PJdBgAsQ5a1JSMsYpVv9HMZFK0BbTgr79C72PcyqfknWrWLmCigK9XyCbYs4Jej0mvzNpX5z0r1p0qUX_rqWwbw6Gc7cD90g-9FCAdwfAZGuGPplgff7DNVwpXJKs0enCffcD7P-9UZ9fbx9W14vC5wl-PCpM-qqFZJLru9uNxtvb9tOGM33FfgJBtrMT</recordid><startdate>201111</startdate><enddate>201111</enddate><creator>Migliorini, Elisa</creator><creator>Grenci, Gianluca</creator><creator>Ban, Jelena</creator><creator>Pozzato, Alessandro</creator><creator>Tormen, Massimo</creator><creator>Lazzarino, Marco</creator><creator>Torre, Vincent</creator><creator>Ruaro, Maria Elisabetta</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope></search><sort><creationdate>201111</creationdate><title>Acceleration of neuronal precursors differentiation induced by substrate nanotopography</title><author>Migliorini, Elisa ; Grenci, Gianluca ; Ban, Jelena ; Pozzato, Alessandro ; Tormen, Massimo ; Lazzarino, Marco ; Torre, Vincent ; Ruaro, Maria Elisabetta</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5512-3a345a5d11e433c7d14fcc9f50e0beeed629b921b2973cad29d7c1c64aa17a83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Differentiation</topic><topic>Cell growth</topic><topic>Cell Proliferation</topic><topic>Culture</topic><topic>Differentiation</topic><topic>Dimethylpolysiloxanes</topic><topic>Diverse techniques</topic><topic>embryonic stem cells</topic><topic>Embryonic Stem Cells - physiology</topic><topic>Fundamental and applied biological sciences. 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| subjects | Animals Biochemistry Biological and medical sciences Biotechnology Cell Culture Techniques - methods Cell Differentiation Cell growth Cell Proliferation Culture Differentiation Dimethylpolysiloxanes Diverse techniques embryonic stem cells Embryonic Stem Cells - physiology Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry Industrial applications and implications. Economical aspects Mice Miscellaneous Molecular and cellular biology Nanocomposites Nanomaterials Nanostructure Nanostructures nanotopography neuronal differentiation Neurons Neurons - physiology pillar Pillars Polydimethylsiloxane Precursors Silicone resins Stem cells Surface Properties |
| title | Acceleration of neuronal precursors differentiation induced by substrate nanotopography |
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