Aptamer-Assisted Gold Nanoparticles/PEDOT Platform for Ultrasensitive Detection of LPS
Neatly arranged gold nanoparticles (AuNPs) were directly electrodeposited on an electrochemically polymerized self‐assembled monolayer (SAM) of thiol‐functionalized 3,4‐ethylenedioxythiophene (EDOT) derivative, EDTMSHA. A thiolated single‐stranded DNA (ssDNA) aptamer with high specificity to LPS was...
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| Veröffentlicht in: | Electroanalysis (New York, N.Y.) N.Y.), 2013-02, Vol.25 (2), p.380-386 |
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| container_title | Electroanalysis (New York, N.Y.) |
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| creator | Su, Wenqiong Cho, MiSuk Nam, Jae-Do Choe, Woo-Seok Lee, Youngkwan |
| description | Neatly arranged gold nanoparticles (AuNPs) were directly electrodeposited on an electrochemically polymerized self‐assembled monolayer (SAM) of thiol‐functionalized 3,4‐ethylenedioxythiophene (EDOT) derivative, EDTMSHA. A thiolated single‐stranded DNA (ssDNA) aptamer with high specificity to LPS was immobilized on the AuNPs/conducting polymer composite film, serving as sensing platform for LPS detection. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), scanning electron microscope (SEM), and atomic force microscopy (AFM) were utilized to characterize the modification and detection processes. The electron transfer resistance was found to have a linear relationship with LPS concentration from 0.1 pg/mL to 1 ng/mL. |
| doi_str_mv | 10.1002/elan.201200453 |
| format | Article |
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A thiolated single‐stranded DNA (ssDNA) aptamer with high specificity to LPS was immobilized on the AuNPs/conducting polymer composite film, serving as sensing platform for LPS detection. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), scanning electron microscope (SEM), and atomic force microscopy (AFM) were utilized to characterize the modification and detection processes. The electron transfer resistance was found to have a linear relationship with LPS concentration from 0.1 pg/mL to 1 ng/mL.</description><identifier>ISSN: 1040-0397</identifier><identifier>EISSN: 1521-4109</identifier><identifier>DOI: 10.1002/elan.201200453</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Acoustic microscopes ; Atomic force microscopy ; Conducting polymer ; Derivatives ; EDTMSHA ; Electrochemical impedance spectroscopy ; Gold ; Gold nanoparticles ; LPS ; Nanoparticles ; PEDOT ; Platforms ; Scanning electron microscopy ; ssDNA aptamers</subject><ispartof>Electroanalysis (New York, N.Y.), 2013-02, Vol.25 (2), p.380-386</ispartof><rights>Copyright © 2013 WILEY‐VCH Verlag GmbH & Co. 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The electron transfer resistance was found to have a linear relationship with LPS concentration from 0.1 pg/mL to 1 ng/mL.</description><subject>Acoustic microscopes</subject><subject>Atomic force microscopy</subject><subject>Conducting polymer</subject><subject>Derivatives</subject><subject>EDTMSHA</subject><subject>Electrochemical impedance spectroscopy</subject><subject>Gold</subject><subject>Gold nanoparticles</subject><subject>LPS</subject><subject>Nanoparticles</subject><subject>PEDOT</subject><subject>Platforms</subject><subject>Scanning electron microscopy</subject><subject>ssDNA aptamers</subject><issn>1040-0397</issn><issn>1521-4109</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqFkD1PwzAQhiMEEqWwMmdkSeuPpI7HqC0FKS1F9IPNcu2zFEiTYLtA_z2piio2lrvT6X3e4QmCW4x6GCHSh1JWPYIwQShO6FnQwQnBUYwRP29vFKMIUc4ugyvn3hBCfBDzTrDKGi-3YKPMucJ50OGkLnU4k1XdSOsLVYLrz8ejp0U4L6U3td2G7QiXpbfSQeUKX3xCOAIPyhd1FdYmzOcv18GFkaWDm9_dDZb348XwIcqfJo_DLI9UTAY0kkwjzjZSm3STgG5_kCiebsgGJxgpOWA45sQQpY02SuoU0oTRRBMqAaSJaTe4O_Y2tv7YgfNiWzgFZasC6p0TuG2ghDNG22jvGFW2ds6CEY0tttLuBUbiIFAcBIqTwBbgR-CrKGH_T1qM82z2l42O7MHp94mV9l0MGGWJWM8mYh6vnqfp9FWs6Q_f-4T8</recordid><startdate>201302</startdate><enddate>201302</enddate><creator>Su, Wenqiong</creator><creator>Cho, MiSuk</creator><creator>Nam, Jae-Do</creator><creator>Choe, Woo-Seok</creator><creator>Lee, Youngkwan</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope></search><sort><creationdate>201302</creationdate><title>Aptamer-Assisted Gold Nanoparticles/PEDOT Platform for Ultrasensitive Detection of LPS</title><author>Su, Wenqiong ; Cho, MiSuk ; Nam, Jae-Do ; Choe, Woo-Seok ; Lee, Youngkwan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4263-a7d097badf8b5ed426e5c98b2b1510ca671492f2cdfdfcad8e85735d23aeeaf43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Acoustic microscopes</topic><topic>Atomic force microscopy</topic><topic>Conducting polymer</topic><topic>Derivatives</topic><topic>EDTMSHA</topic><topic>Electrochemical impedance spectroscopy</topic><topic>Gold</topic><topic>Gold nanoparticles</topic><topic>LPS</topic><topic>Nanoparticles</topic><topic>PEDOT</topic><topic>Platforms</topic><topic>Scanning electron microscopy</topic><topic>ssDNA aptamers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Su, Wenqiong</creatorcontrib><creatorcontrib>Cho, MiSuk</creatorcontrib><creatorcontrib>Nam, Jae-Do</creatorcontrib><creatorcontrib>Choe, Woo-Seok</creatorcontrib><creatorcontrib>Lee, Youngkwan</creatorcontrib><collection>Istex</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Electroanalysis (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Su, Wenqiong</au><au>Cho, MiSuk</au><au>Nam, Jae-Do</au><au>Choe, Woo-Seok</au><au>Lee, Youngkwan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aptamer-Assisted Gold Nanoparticles/PEDOT Platform for Ultrasensitive Detection of LPS</atitle><jtitle>Electroanalysis (New York, N.Y.)</jtitle><addtitle>Electroanalysis</addtitle><date>2013-02</date><risdate>2013</risdate><volume>25</volume><issue>2</issue><spage>380</spage><epage>386</epage><pages>380-386</pages><issn>1040-0397</issn><eissn>1521-4109</eissn><abstract>Neatly arranged gold nanoparticles (AuNPs) were directly electrodeposited on an electrochemically polymerized self‐assembled monolayer (SAM) of thiol‐functionalized 3,4‐ethylenedioxythiophene (EDOT) derivative, EDTMSHA. A thiolated single‐stranded DNA (ssDNA) aptamer with high specificity to LPS was immobilized on the AuNPs/conducting polymer composite film, serving as sensing platform for LPS detection. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), scanning electron microscope (SEM), and atomic force microscopy (AFM) were utilized to characterize the modification and detection processes. The electron transfer resistance was found to have a linear relationship with LPS concentration from 0.1 pg/mL to 1 ng/mL.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><doi>10.1002/elan.201200453</doi><tpages>7</tpages></addata></record> |
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| ispartof | Electroanalysis (New York, N.Y.), 2013-02, Vol.25 (2), p.380-386 |
| issn | 1040-0397 1521-4109 |
| language | eng |
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| source | Wiley Online Library All Journals |
| subjects | Acoustic microscopes Atomic force microscopy Conducting polymer Derivatives EDTMSHA Electrochemical impedance spectroscopy Gold Gold nanoparticles LPS Nanoparticles PEDOT Platforms Scanning electron microscopy ssDNA aptamers |
| title | Aptamer-Assisted Gold Nanoparticles/PEDOT Platform for Ultrasensitive Detection of LPS |
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