Exon 8 amplification of epidermal growth factor receptor (EGFR) in invasive breast carcinomas
Souka E, Alexiadis E, Theohari I, Giannopoulou I, Papadimitriou C & Nakopoulou L (2012) Histopathology 61, 644–651 Exon 8 amplification of epidermal growth factor receptor (EGFR) in invasive breast carcinomas Aims: The rate of EGFR amplification in breast cancer ranges between 0% and 15%. Rece...
Gespeichert in:
| Veröffentlicht in: | Histopathology 2012-10, Vol.61 (4), p.644-651 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 651 |
|---|---|
| container_issue | 4 |
| container_start_page | 644 |
| container_title | Histopathology |
| container_volume | 61 |
| creator | Souka, Efthimia Alexiadis, Evaggelos Theohari, Irini Giannopoulou, Ioanna Papadimitriou, Christos Nakopoulou, Lydia |
| description | Souka E, Alexiadis E, Theohari I, Giannopoulou I, Papadimitriou C & Nakopoulou L
(2012) Histopathology 61, 644–651
Exon 8 amplification of epidermal growth factor receptor (EGFR) in invasive breast carcinomas
Aims: The rate of EGFR amplification in breast cancer ranges between 0% and 15%. Recent studies have focused on the amplification status of cytosine–adenine (CA) repeats in intron 1 of the gene and correlated it with increased EGFR protein. The aim of this study was to investigate, for the first time, the significance of coding exon 8 amplification of EGFR in invasive breast cancer.
Methods and results: We investigated, by means of real‐time polymerase chain reaction (PCR), the amplification status of exon 8 of the EGFR gene in 148 paraffin‐embedded tissue sections, 115 with invasive breast carcinoma and 33 controls. Immunohistochemistry was utilized to detect EGFR and human epidermal growth factor receptor 2 (HER2) expression. Univariate and multivariate statistical analyses were performed for the evaluation of our results. EGFR amplification was observed in 7.8% of the patients, and EGFR was immunodetected in 9.6%. EGFR amplification was correlated positively with EGFR expression (P |
| doi_str_mv | 10.1111/j.1365-2559.2012.04315.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1671222727</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1671222727</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4655-b00a9806e625b63c5b57d9f650c7b25d3e4f89f2d69b3a6543821c660d518fb23</originalsourceid><addsrcrecordid>eNqNkV1rFDEUhoModm39CxIQoV7MNB-bj7nwopTttlAsVm1BkJDJJJp1ZjIms-3235tx1xW88hDICXne5OU9AECMSpzrZFViyllBGKtKgjAp0ZxiVm6egNn-4imYIYqqAmEuDsCLlFYIYUEJeQ4OCJGSYCJn4OtiE3oooe6G1jtv9OjzOThoB9_Y2OkWfovhYfwOnTZjiDBaY4epOV4sz2_eQt_nda-Tv7ewjlanERodje9Dp9MReOZ0m-zL3X4IPp8vPp1dFFfXy8uz06vCzDljRY2QriTilhNWc2pYzURTOc6QETVhDbVzJytHGl7VVHM2p9m94Rw1DEtXE3oIjrfvDjH8XNs0qs4nY9tW9zask8oRYEKIICKjr_9BV2Ed--xOYcG5JBJXMlNyS5kYUorWqSH6TsdHhZGaRqBWakpaTUmraQTq9wjUJktf7T5Y151t9sI_mWfgzQ7QyejWRd0bn_5ynPEqV-bebbkH39rH_zagLi4_Tl3WF1u9T6Pd7PU6_lBcUMHU3fulurvFXMrbL-oD_QUZwK7c</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1766828198</pqid></control><display><type>article</type><title>Exon 8 amplification of epidermal growth factor receptor (EGFR) in invasive breast carcinomas</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Souka, Efthimia ; Alexiadis, Evaggelos ; Theohari, Irini ; Giannopoulou, Ioanna ; Papadimitriou, Christos ; Nakopoulou, Lydia</creator><creatorcontrib>Souka, Efthimia ; Alexiadis, Evaggelos ; Theohari, Irini ; Giannopoulou, Ioanna ; Papadimitriou, Christos ; Nakopoulou, Lydia</creatorcontrib><description>Souka E, Alexiadis E, Theohari I, Giannopoulou I, Papadimitriou C & Nakopoulou L
(2012) Histopathology 61, 644–651
Exon 8 amplification of epidermal growth factor receptor (EGFR) in invasive breast carcinomas
Aims: The rate of EGFR amplification in breast cancer ranges between 0% and 15%. Recent studies have focused on the amplification status of cytosine–adenine (CA) repeats in intron 1 of the gene and correlated it with increased EGFR protein. The aim of this study was to investigate, for the first time, the significance of coding exon 8 amplification of EGFR in invasive breast cancer.
Methods and results: We investigated, by means of real‐time polymerase chain reaction (PCR), the amplification status of exon 8 of the EGFR gene in 148 paraffin‐embedded tissue sections, 115 with invasive breast carcinoma and 33 controls. Immunohistochemistry was utilized to detect EGFR and human epidermal growth factor receptor 2 (HER2) expression. Univariate and multivariate statistical analyses were performed for the evaluation of our results. EGFR amplification was observed in 7.8% of the patients, and EGFR was immunodetected in 9.6%. EGFR amplification was correlated positively with EGFR expression (P < 0.0001), HER2 expression (P = 0.023), coexpression of EGFR/HER2 (P < 0.0001) and nuclear grade (P = 0.047), and inversely with ER protein expression (P = 0.047).
Conclusions: It appears that amplification of the coding sequence exon 8 exhibits similar biological behaviour to amplification of the regulatory sequence in intron 1, leading to elevated levels of EGFR protein.</description><identifier>ISSN: 0309-0167</identifier><identifier>EISSN: 1365-2559</identifier><identifier>DOI: 10.1111/j.1365-2559.2012.04315.x</identifier><identifier>PMID: 22882128</identifier><identifier>CODEN: HISTDD</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Biological and medical sciences ; Biomarkers, Tumor ; breast cancer ; Breast Neoplasms - genetics ; Breast Neoplasms - pathology ; epidermal growth factor receptor ; exon 8 ; Exons ; Female ; Gene Amplification ; Genes, erbB-1 ; Gynecology. Andrology. Obstetrics ; Humans ; Immunohistochemistry ; Investigative techniques, diagnostic techniques (general aspects) ; Mammary gland diseases ; Medical sciences ; Neoplasm Invasiveness ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; real-time PCR ; Real-Time Polymerase Chain Reaction ; Receptor, Epidermal Growth Factor - genetics ; Tumors</subject><ispartof>Histopathology, 2012-10, Vol.61 (4), p.644-651</ispartof><rights>2012 Blackwell Publishing Ltd</rights><rights>2015 INIST-CNRS</rights><rights>2012 Blackwell Publishing Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4655-b00a9806e625b63c5b57d9f650c7b25d3e4f89f2d69b3a6543821c660d518fb23</citedby><cites>FETCH-LOGICAL-c4655-b00a9806e625b63c5b57d9f650c7b25d3e4f89f2d69b3a6543821c660d518fb23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2559.2012.04315.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2559.2012.04315.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27911,27912,45561,45562</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26569999$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22882128$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Souka, Efthimia</creatorcontrib><creatorcontrib>Alexiadis, Evaggelos</creatorcontrib><creatorcontrib>Theohari, Irini</creatorcontrib><creatorcontrib>Giannopoulou, Ioanna</creatorcontrib><creatorcontrib>Papadimitriou, Christos</creatorcontrib><creatorcontrib>Nakopoulou, Lydia</creatorcontrib><title>Exon 8 amplification of epidermal growth factor receptor (EGFR) in invasive breast carcinomas</title><title>Histopathology</title><addtitle>Histopathology</addtitle><description>Souka E, Alexiadis E, Theohari I, Giannopoulou I, Papadimitriou C & Nakopoulou L
(2012) Histopathology 61, 644–651
Exon 8 amplification of epidermal growth factor receptor (EGFR) in invasive breast carcinomas
Aims: The rate of EGFR amplification in breast cancer ranges between 0% and 15%. Recent studies have focused on the amplification status of cytosine–adenine (CA) repeats in intron 1 of the gene and correlated it with increased EGFR protein. The aim of this study was to investigate, for the first time, the significance of coding exon 8 amplification of EGFR in invasive breast cancer.
Methods and results: We investigated, by means of real‐time polymerase chain reaction (PCR), the amplification status of exon 8 of the EGFR gene in 148 paraffin‐embedded tissue sections, 115 with invasive breast carcinoma and 33 controls. Immunohistochemistry was utilized to detect EGFR and human epidermal growth factor receptor 2 (HER2) expression. Univariate and multivariate statistical analyses were performed for the evaluation of our results. EGFR amplification was observed in 7.8% of the patients, and EGFR was immunodetected in 9.6%. EGFR amplification was correlated positively with EGFR expression (P < 0.0001), HER2 expression (P = 0.023), coexpression of EGFR/HER2 (P < 0.0001) and nuclear grade (P = 0.047), and inversely with ER protein expression (P = 0.047).
Conclusions: It appears that amplification of the coding sequence exon 8 exhibits similar biological behaviour to amplification of the regulatory sequence in intron 1, leading to elevated levels of EGFR protein.</description><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor</subject><subject>breast cancer</subject><subject>Breast Neoplasms - genetics</subject><subject>Breast Neoplasms - pathology</subject><subject>epidermal growth factor receptor</subject><subject>exon 8</subject><subject>Exons</subject><subject>Female</subject><subject>Gene Amplification</subject><subject>Genes, erbB-1</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Mammary gland diseases</subject><subject>Medical sciences</subject><subject>Neoplasm Invasiveness</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>real-time PCR</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Receptor, Epidermal Growth Factor - genetics</subject><subject>Tumors</subject><issn>0309-0167</issn><issn>1365-2559</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV1rFDEUhoModm39CxIQoV7MNB-bj7nwopTttlAsVm1BkJDJJJp1ZjIms-3235tx1xW88hDICXne5OU9AECMSpzrZFViyllBGKtKgjAp0ZxiVm6egNn-4imYIYqqAmEuDsCLlFYIYUEJeQ4OCJGSYCJn4OtiE3oooe6G1jtv9OjzOThoB9_Y2OkWfovhYfwOnTZjiDBaY4epOV4sz2_eQt_nda-Tv7ewjlanERodje9Dp9MReOZ0m-zL3X4IPp8vPp1dFFfXy8uz06vCzDljRY2QriTilhNWc2pYzURTOc6QETVhDbVzJytHGl7VVHM2p9m94Rw1DEtXE3oIjrfvDjH8XNs0qs4nY9tW9zask8oRYEKIICKjr_9BV2Ed--xOYcG5JBJXMlNyS5kYUorWqSH6TsdHhZGaRqBWakpaTUmraQTq9wjUJktf7T5Y151t9sI_mWfgzQ7QyejWRd0bn_5ynPEqV-bebbkH39rH_zagLi4_Tl3WF1u9T6Pd7PU6_lBcUMHU3fulurvFXMrbL-oD_QUZwK7c</recordid><startdate>201210</startdate><enddate>201210</enddate><creator>Souka, Efthimia</creator><creator>Alexiadis, Evaggelos</creator><creator>Theohari, Irini</creator><creator>Giannopoulou, Ioanna</creator><creator>Papadimitriou, Christos</creator><creator>Nakopoulou, Lydia</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201210</creationdate><title>Exon 8 amplification of epidermal growth factor receptor (EGFR) in invasive breast carcinomas</title><author>Souka, Efthimia ; Alexiadis, Evaggelos ; Theohari, Irini ; Giannopoulou, Ioanna ; Papadimitriou, Christos ; Nakopoulou, Lydia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4655-b00a9806e625b63c5b57d9f650c7b25d3e4f89f2d69b3a6543821c660d518fb23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor</topic><topic>breast cancer</topic><topic>Breast Neoplasms - genetics</topic><topic>Breast Neoplasms - pathology</topic><topic>epidermal growth factor receptor</topic><topic>exon 8</topic><topic>Exons</topic><topic>Female</topic><topic>Gene Amplification</topic><topic>Genes, erbB-1</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Mammary gland diseases</topic><topic>Medical sciences</topic><topic>Neoplasm Invasiveness</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>real-time PCR</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Receptor, Epidermal Growth Factor - genetics</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Souka, Efthimia</creatorcontrib><creatorcontrib>Alexiadis, Evaggelos</creatorcontrib><creatorcontrib>Theohari, Irini</creatorcontrib><creatorcontrib>Giannopoulou, Ioanna</creatorcontrib><creatorcontrib>Papadimitriou, Christos</creatorcontrib><creatorcontrib>Nakopoulou, Lydia</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Histopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Souka, Efthimia</au><au>Alexiadis, Evaggelos</au><au>Theohari, Irini</au><au>Giannopoulou, Ioanna</au><au>Papadimitriou, Christos</au><au>Nakopoulou, Lydia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exon 8 amplification of epidermal growth factor receptor (EGFR) in invasive breast carcinomas</atitle><jtitle>Histopathology</jtitle><addtitle>Histopathology</addtitle><date>2012-10</date><risdate>2012</risdate><volume>61</volume><issue>4</issue><spage>644</spage><epage>651</epage><pages>644-651</pages><issn>0309-0167</issn><eissn>1365-2559</eissn><coden>HISTDD</coden><abstract>Souka E, Alexiadis E, Theohari I, Giannopoulou I, Papadimitriou C & Nakopoulou L
(2012) Histopathology 61, 644–651
Exon 8 amplification of epidermal growth factor receptor (EGFR) in invasive breast carcinomas
Aims: The rate of EGFR amplification in breast cancer ranges between 0% and 15%. Recent studies have focused on the amplification status of cytosine–adenine (CA) repeats in intron 1 of the gene and correlated it with increased EGFR protein. The aim of this study was to investigate, for the first time, the significance of coding exon 8 amplification of EGFR in invasive breast cancer.
Methods and results: We investigated, by means of real‐time polymerase chain reaction (PCR), the amplification status of exon 8 of the EGFR gene in 148 paraffin‐embedded tissue sections, 115 with invasive breast carcinoma and 33 controls. Immunohistochemistry was utilized to detect EGFR and human epidermal growth factor receptor 2 (HER2) expression. Univariate and multivariate statistical analyses were performed for the evaluation of our results. EGFR amplification was observed in 7.8% of the patients, and EGFR was immunodetected in 9.6%. EGFR amplification was correlated positively with EGFR expression (P < 0.0001), HER2 expression (P = 0.023), coexpression of EGFR/HER2 (P < 0.0001) and nuclear grade (P = 0.047), and inversely with ER protein expression (P = 0.047).
Conclusions: It appears that amplification of the coding sequence exon 8 exhibits similar biological behaviour to amplification of the regulatory sequence in intron 1, leading to elevated levels of EGFR protein.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>22882128</pmid><doi>10.1111/j.1365-2559.2012.04315.x</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0309-0167 |
| ispartof | Histopathology, 2012-10, Vol.61 (4), p.644-651 |
| issn | 0309-0167 1365-2559 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1671222727 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Biological and medical sciences Biomarkers, Tumor breast cancer Breast Neoplasms - genetics Breast Neoplasms - pathology epidermal growth factor receptor exon 8 Exons Female Gene Amplification Genes, erbB-1 Gynecology. Andrology. Obstetrics Humans Immunohistochemistry Investigative techniques, diagnostic techniques (general aspects) Mammary gland diseases Medical sciences Neoplasm Invasiveness Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques real-time PCR Real-Time Polymerase Chain Reaction Receptor, Epidermal Growth Factor - genetics Tumors |
| title | Exon 8 amplification of epidermal growth factor receptor (EGFR) in invasive breast carcinomas |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T15%3A57%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Exon%208%20amplification%20of%20epidermal%20growth%20factor%20receptor%20(EGFR)%20in%20invasive%20breast%20carcinomas&rft.jtitle=Histopathology&rft.au=Souka,%20Efthimia&rft.date=2012-10&rft.volume=61&rft.issue=4&rft.spage=644&rft.epage=651&rft.pages=644-651&rft.issn=0309-0167&rft.eissn=1365-2559&rft.coden=HISTDD&rft_id=info:doi/10.1111/j.1365-2559.2012.04315.x&rft_dat=%3Cproquest_cross%3E1671222727%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1766828198&rft_id=info:pmid/22882128&rfr_iscdi=true |