Effect of diethylcarbamazine citrate and omega-3 fatty acids on trimellitic anhydride-induced rat skin allergy
Diethylcarbamazine citrate (DEC) is the drug most widely used in the treatment of lymphatic filariasis. Omega-3 fatty acids (ω-3 FAs) are essential polyunsaturated fatty acids and commonly found in marine oils. Both have been applied in treatment of inflammatory diseases but anti-allergic effects sh...
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| Veröffentlicht in: | Asian Pacific journal of allergy and immunology 2015-03, Vol.33 (1), p.33-41 |
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| creator | Abdel Latif, Mahmoud Abdul-Hamid, Manal Galaly, Sanaa R |
| description | Diethylcarbamazine citrate (DEC) is the drug most widely used in the treatment of lymphatic filariasis. Omega-3 fatty acids (ω-3 FAs) are essential polyunsaturated fatty acids and commonly found in marine oils. Both have been applied in treatment of inflammatory diseases but anti-allergic effects should be investigated.
The present study was performed to test the effect of both DEC and ω -3 FAs on Trimellitic anhydride (TMA) - induced rat skin allergy.
In vivo experiment was executed in white albino rats using 100 and 600 mg/ Kg body weight of DEC and ω-3 FAs, respectively in treatment. Ear thickness of sensitized rats to TMA was monitored after challenge. Blood eosinophilia was determined using differential leukocyte count while the appearance of mast cells, eosinophils and collagen fibers in skin tissue were investigated using specific stains. Colorimetric assay of NO was performed in homogenized ears, while expression of inducible nitric oxide synthase (iNOS) was detected using immunohistochemistry.
Ear thickness showed a significant (p < 0.05) reduction in both of DEC and ω-3 FAs treated groups. Blood eosinophilia and skin eosinophils were significantly (p < 0.001) decreased by DEC and ?-3 FAs, while the decrease of skin mast cells was only significant (p < 0.01) when ω-3 FAs applied. The expression of iNOS and intensity of stained collagen fibers were decreased obviously by ω-3 FAs but less by DEC treatment. Histopathological observations were more normal in ω-3 FAs than DEC treated groups.
ω-3 FAs was more potent antiallergic substance against TMA-induced dermatitis than DEC. |
| doi_str_mv | 10.12932/AP0499.33.1.2015 |
| format | Article |
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The present study was performed to test the effect of both DEC and ω -3 FAs on Trimellitic anhydride (TMA) - induced rat skin allergy.
In vivo experiment was executed in white albino rats using 100 and 600 mg/ Kg body weight of DEC and ω-3 FAs, respectively in treatment. Ear thickness of sensitized rats to TMA was monitored after challenge. Blood eosinophilia was determined using differential leukocyte count while the appearance of mast cells, eosinophils and collagen fibers in skin tissue were investigated using specific stains. Colorimetric assay of NO was performed in homogenized ears, while expression of inducible nitric oxide synthase (iNOS) was detected using immunohistochemistry.
Ear thickness showed a significant (p < 0.05) reduction in both of DEC and ω-3 FAs treated groups. Blood eosinophilia and skin eosinophils were significantly (p < 0.001) decreased by DEC and ?-3 FAs, while the decrease of skin mast cells was only significant (p < 0.01) when ω-3 FAs applied. The expression of iNOS and intensity of stained collagen fibers were decreased obviously by ω-3 FAs but less by DEC treatment. Histopathological observations were more normal in ω-3 FAs than DEC treated groups.
ω-3 FAs was more potent antiallergic substance against TMA-induced dermatitis than DEC.</description><identifier>ISSN: 0125-877X</identifier><identifier>DOI: 10.12932/AP0499.33.1.2015</identifier><identifier>PMID: 25840632</identifier><language>eng</language><publisher>Thailand: The Allergy and Immunology Society</publisher><subject>Administration, Cutaneous ; Allergens - adverse effects ; Animals ; Anti-Allergic Agents - pharmacology ; Collagen - antagonists & inhibitors ; Collagen - biosynthesis ; Diethylcarbamazine - pharmacology ; Ear ; Eosinophils - drug effects ; Eosinophils - immunology ; Eosinophils - pathology ; Fatty Acids, Omega-3 - pharmacology ; Gene Expression ; Humans ; Hypersensitivity - drug therapy ; Hypersensitivity - etiology ; Hypersensitivity - immunology ; Hypersensitivity - pathology ; Male ; Mast Cells - drug effects ; Mast Cells - immunology ; Mast Cells - pathology ; Nitric Oxide - antagonists & inhibitors ; Nitric Oxide - metabolism ; Nitric Oxide Synthase Type II - antagonists & inhibitors ; Nitric Oxide Synthase Type II - genetics ; Nitric Oxide Synthase Type II - metabolism ; Phthalic Anhydrides - adverse effects ; Rats ; Skin - drug effects ; Skin - immunology ; Skin - pathology</subject><ispartof>Asian Pacific journal of allergy and immunology, 2015-03, Vol.33 (1), p.33-41</ispartof><rights>Copyright The Allergy and Immunology Society of Thailand Mar 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25840632$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abdel Latif, Mahmoud</creatorcontrib><creatorcontrib>Abdul-Hamid, Manal</creatorcontrib><creatorcontrib>Galaly, Sanaa R</creatorcontrib><title>Effect of diethylcarbamazine citrate and omega-3 fatty acids on trimellitic anhydride-induced rat skin allergy</title><title>Asian Pacific journal of allergy and immunology</title><addtitle>Asian Pac J Allergy Immunol</addtitle><description>Diethylcarbamazine citrate (DEC) is the drug most widely used in the treatment of lymphatic filariasis. Omega-3 fatty acids (ω-3 FAs) are essential polyunsaturated fatty acids and commonly found in marine oils. Both have been applied in treatment of inflammatory diseases but anti-allergic effects should be investigated.
The present study was performed to test the effect of both DEC and ω -3 FAs on Trimellitic anhydride (TMA) - induced rat skin allergy.
In vivo experiment was executed in white albino rats using 100 and 600 mg/ Kg body weight of DEC and ω-3 FAs, respectively in treatment. Ear thickness of sensitized rats to TMA was monitored after challenge. Blood eosinophilia was determined using differential leukocyte count while the appearance of mast cells, eosinophils and collagen fibers in skin tissue were investigated using specific stains. Colorimetric assay of NO was performed in homogenized ears, while expression of inducible nitric oxide synthase (iNOS) was detected using immunohistochemistry.
Ear thickness showed a significant (p < 0.05) reduction in both of DEC and ω-3 FAs treated groups. Blood eosinophilia and skin eosinophils were significantly (p < 0.001) decreased by DEC and ?-3 FAs, while the decrease of skin mast cells was only significant (p < 0.01) when ω-3 FAs applied. The expression of iNOS and intensity of stained collagen fibers were decreased obviously by ω-3 FAs but less by DEC treatment. Histopathological observations were more normal in ω-3 FAs than DEC treated groups.
ω-3 FAs was more potent antiallergic substance against TMA-induced dermatitis than DEC.</description><subject>Administration, Cutaneous</subject><subject>Allergens - adverse effects</subject><subject>Animals</subject><subject>Anti-Allergic Agents - pharmacology</subject><subject>Collagen - antagonists & inhibitors</subject><subject>Collagen - biosynthesis</subject><subject>Diethylcarbamazine - pharmacology</subject><subject>Ear</subject><subject>Eosinophils - drug effects</subject><subject>Eosinophils - immunology</subject><subject>Eosinophils - pathology</subject><subject>Fatty Acids, Omega-3 - pharmacology</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Hypersensitivity - drug therapy</subject><subject>Hypersensitivity - etiology</subject><subject>Hypersensitivity - immunology</subject><subject>Hypersensitivity - pathology</subject><subject>Male</subject><subject>Mast Cells - drug effects</subject><subject>Mast Cells - immunology</subject><subject>Mast Cells - pathology</subject><subject>Nitric Oxide - antagonists & inhibitors</subject><subject>Nitric Oxide - metabolism</subject><subject>Nitric Oxide Synthase Type II - antagonists & inhibitors</subject><subject>Nitric Oxide Synthase Type II - genetics</subject><subject>Nitric Oxide Synthase Type II - metabolism</subject><subject>Phthalic Anhydrides - adverse effects</subject><subject>Rats</subject><subject>Skin - drug effects</subject><subject>Skin - immunology</subject><subject>Skin - pathology</subject><issn>0125-877X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkTtLBTEQhVMoXlF_gI0EbGz2mkl2k91SxBcIWijYhdxk9hrdhybZYv31Rq9aOM0w8J3DzBxCDoEtgTeCn57ds7JplkIsYckZVFtklwGvilqppwU5iPGF5ZIAdVXukAWv6pJJwXfJcNG2aBMdW-o8pue5syasTG8-_IDU-hRMQmoGR8ce16YQtDUpzdRY7yIdB5qC77HrfPI2Y8-zC95h4Qc3WXQ0q2l89QM1XYdhPe-T7dZ0EQ9--h55vLx4OL8ubu-ubs7PbgvLa0gFSOQSK8GwbkBWwoJiDixXK1lKXteclaiwQVU1zhlV2qpGK1kemOAGpdgjJxvftzC-TxiT7n20eU8z4DhFDVIB50wpldHjf-jLOIUhb_dFyZIpCU2mYEPZMMYYsNVv-XATZg1Mf2egNxloITTorwyy5ujHeVr16P4Uv98Xn3j2gy0</recordid><startdate>20150301</startdate><enddate>20150301</enddate><creator>Abdel Latif, Mahmoud</creator><creator>Abdul-Hamid, Manal</creator><creator>Galaly, Sanaa R</creator><general>The Allergy and Immunology Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BVBZV</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20150301</creationdate><title>Effect of diethylcarbamazine citrate and omega-3 fatty acids on trimellitic anhydride-induced rat skin allergy</title><author>Abdel Latif, Mahmoud ; Abdul-Hamid, Manal ; Galaly, Sanaa R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c281t-16e26e530e891653c170d1c27b646288204e7e9e759dda74c58ec609dd032ae63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Administration, Cutaneous</topic><topic>Allergens - adverse effects</topic><topic>Animals</topic><topic>Anti-Allergic Agents - pharmacology</topic><topic>Collagen - antagonists & inhibitors</topic><topic>Collagen - biosynthesis</topic><topic>Diethylcarbamazine - pharmacology</topic><topic>Ear</topic><topic>Eosinophils - drug effects</topic><topic>Eosinophils - immunology</topic><topic>Eosinophils - pathology</topic><topic>Fatty Acids, Omega-3 - pharmacology</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Hypersensitivity - drug therapy</topic><topic>Hypersensitivity - etiology</topic><topic>Hypersensitivity - immunology</topic><topic>Hypersensitivity - pathology</topic><topic>Male</topic><topic>Mast Cells - drug effects</topic><topic>Mast Cells - immunology</topic><topic>Mast Cells - pathology</topic><topic>Nitric Oxide - antagonists & inhibitors</topic><topic>Nitric Oxide - metabolism</topic><topic>Nitric Oxide Synthase Type II - antagonists & inhibitors</topic><topic>Nitric Oxide Synthase Type II - genetics</topic><topic>Nitric Oxide Synthase Type II - metabolism</topic><topic>Phthalic Anhydrides - adverse effects</topic><topic>Rats</topic><topic>Skin - drug effects</topic><topic>Skin - immunology</topic><topic>Skin - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abdel Latif, Mahmoud</creatorcontrib><creatorcontrib>Abdul-Hamid, Manal</creatorcontrib><creatorcontrib>Galaly, Sanaa R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>East & South Asia Database</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Asian Pacific journal of allergy and immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abdel Latif, Mahmoud</au><au>Abdul-Hamid, Manal</au><au>Galaly, Sanaa R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of diethylcarbamazine citrate and omega-3 fatty acids on trimellitic anhydride-induced rat skin allergy</atitle><jtitle>Asian Pacific journal of allergy and immunology</jtitle><addtitle>Asian Pac J Allergy Immunol</addtitle><date>2015-03-01</date><risdate>2015</risdate><volume>33</volume><issue>1</issue><spage>33</spage><epage>41</epage><pages>33-41</pages><issn>0125-877X</issn><abstract>Diethylcarbamazine citrate (DEC) is the drug most widely used in the treatment of lymphatic filariasis. Omega-3 fatty acids (ω-3 FAs) are essential polyunsaturated fatty acids and commonly found in marine oils. Both have been applied in treatment of inflammatory diseases but anti-allergic effects should be investigated.
The present study was performed to test the effect of both DEC and ω -3 FAs on Trimellitic anhydride (TMA) - induced rat skin allergy.
In vivo experiment was executed in white albino rats using 100 and 600 mg/ Kg body weight of DEC and ω-3 FAs, respectively in treatment. Ear thickness of sensitized rats to TMA was monitored after challenge. Blood eosinophilia was determined using differential leukocyte count while the appearance of mast cells, eosinophils and collagen fibers in skin tissue were investigated using specific stains. Colorimetric assay of NO was performed in homogenized ears, while expression of inducible nitric oxide synthase (iNOS) was detected using immunohistochemistry.
Ear thickness showed a significant (p < 0.05) reduction in both of DEC and ω-3 FAs treated groups. Blood eosinophilia and skin eosinophils were significantly (p < 0.001) decreased by DEC and ?-3 FAs, while the decrease of skin mast cells was only significant (p < 0.01) when ω-3 FAs applied. The expression of iNOS and intensity of stained collagen fibers were decreased obviously by ω-3 FAs but less by DEC treatment. Histopathological observations were more normal in ω-3 FAs than DEC treated groups.
ω-3 FAs was more potent antiallergic substance against TMA-induced dermatitis than DEC.</abstract><cop>Thailand</cop><pub>The Allergy and Immunology Society</pub><pmid>25840632</pmid><doi>10.12932/AP0499.33.1.2015</doi><tpages>9</tpages></addata></record> |
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| subjects | Administration, Cutaneous Allergens - adverse effects Animals Anti-Allergic Agents - pharmacology Collagen - antagonists & inhibitors Collagen - biosynthesis Diethylcarbamazine - pharmacology Ear Eosinophils - drug effects Eosinophils - immunology Eosinophils - pathology Fatty Acids, Omega-3 - pharmacology Gene Expression Humans Hypersensitivity - drug therapy Hypersensitivity - etiology Hypersensitivity - immunology Hypersensitivity - pathology Male Mast Cells - drug effects Mast Cells - immunology Mast Cells - pathology Nitric Oxide - antagonists & inhibitors Nitric Oxide - metabolism Nitric Oxide Synthase Type II - antagonists & inhibitors Nitric Oxide Synthase Type II - genetics Nitric Oxide Synthase Type II - metabolism Phthalic Anhydrides - adverse effects Rats Skin - drug effects Skin - immunology Skin - pathology |
| title | Effect of diethylcarbamazine citrate and omega-3 fatty acids on trimellitic anhydride-induced rat skin allergy |
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