Quantification of in vitro mesenchymal stem cell invasion into tumor spheroids using selective plane illumination microscopy
Mesenchymal stem cell (MSC) homing and integration into tumors are under evaluation for clinical application. This approach requires the identification of conditions for optimal tumor invasion. We describe a tool for the in vitro comparison of parameters influencing invasion. Human MSC added to expe...
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| Veröffentlicht in: | Journal of biomedical optics 2015-04, Vol.20 (4), p.040501-040501 |
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| container_title | Journal of biomedical optics |
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| creator | Rühland, Svenja Wechselberger, Alexandra Spitzweg, Christine Huss, Ralf Nelson, Peter J Harz, Hartmann |
| description | Mesenchymal stem cell (MSC) homing and integration into tumors are under evaluation for clinical application. This approach requires the identification of conditions for optimal tumor invasion. We describe a tool for the in vitro comparison of parameters influencing invasion. Human MSC added to experimental tumor spheroids variably migrates toward the center of the structure. To determine MSC distribution inside the three-dimensional specimen, spatial analysis was performed using selective plane illumination microscopy. A standardized method to quantify and compare the invasion potential of variably treated MSC into experimental tumor environments allows efficient screening for optimizing conditions. |
| doi_str_mv | 10.1117/1.JBO.20.4.040501 |
| format | Article |
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Biomed. Opt</addtitle><description>Mesenchymal stem cell (MSC) homing and integration into tumors are under evaluation for clinical application. This approach requires the identification of conditions for optimal tumor invasion. We describe a tool for the in vitro comparison of parameters influencing invasion. Human MSC added to experimental tumor spheroids variably migrates toward the center of the structure. To determine MSC distribution inside the three-dimensional specimen, spatial analysis was performed using selective plane illumination microscopy. A standardized method to quantify and compare the invasion potential of variably treated MSC into experimental tumor environments allows efficient screening for optimizing conditions.</description><subject>Carcinoma, Hepatocellular - pathology</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement</subject><subject>Cell Tracking - methods</subject><subject>Humans</subject><subject>Lighting - methods</subject><subject>Mesenchymal Stromal Cells - pathology</subject><subject>Microscopy - methods</subject><subject>Neoplasm Invasiveness</subject><subject>Spheroids, Cellular - pathology</subject><issn>1083-3668</issn><issn>1560-2281</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFv1DAQhSMEoqXwA7ggH7kkHduJYx9LodCqUkEqZ8vrTKgrJw62s9IifjzZpvRQUE8eeb73ZuYVxVsKFaW0PabVxYerikFVV1BDA_RZcUgbASVjkj5fapC85ELIg-JVSrcAIIUSL4sD1kiuatYeFr-_zWbMrnfWZBdGEnriRrJ1OQYyYMLR3uwG40nKOBCL3i_trUl71I05kDwPIZI03WAMrktkTm78QRJ6tNltkUzejEic9_PgxnXE4GwMyYZp97p40Ruf8M39e1R8P_t0ffqlvLz6fH56clnaBmQu0fbGSAOq74ArkG3HpUEFVGy4wt7WVDJD207xnhsGrG44CKMaxtWm4Wj5UfF-9Z1i-DljynpwaX_MsluYk6aipYwq0coFpSu63zFF7PUU3WDiTlPQ-9A11UvomoGu9Rr6onl3bz9vBuweFH9TXoBqBdLkUN-GOY7LuU86Xv9P8ID9ctNjzd3fSczOevz68eyf9tT1_A8E6amy</recordid><startdate>20150401</startdate><enddate>20150401</enddate><creator>Rühland, Svenja</creator><creator>Wechselberger, Alexandra</creator><creator>Spitzweg, Christine</creator><creator>Huss, Ralf</creator><creator>Nelson, Peter J</creator><creator>Harz, Hartmann</creator><general>Society of Photo-Optical Instrumentation Engineers</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150401</creationdate><title>Quantification of in vitro mesenchymal stem cell invasion into tumor spheroids using selective plane illumination microscopy</title><author>Rühland, Svenja ; Wechselberger, Alexandra ; Spitzweg, Christine ; Huss, Ralf ; Nelson, Peter J ; Harz, Hartmann</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c508t-ecfaa8a09fd039087d38ae9016b39efc4182a17d93f3a20245306a95239b53ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Carcinoma, Hepatocellular - pathology</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement</topic><topic>Cell Tracking - methods</topic><topic>Humans</topic><topic>Lighting - methods</topic><topic>Mesenchymal Stromal Cells - pathology</topic><topic>Microscopy - methods</topic><topic>Neoplasm Invasiveness</topic><topic>Spheroids, Cellular - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rühland, Svenja</creatorcontrib><creatorcontrib>Wechselberger, Alexandra</creatorcontrib><creatorcontrib>Spitzweg, Christine</creatorcontrib><creatorcontrib>Huss, Ralf</creatorcontrib><creatorcontrib>Nelson, Peter J</creatorcontrib><creatorcontrib>Harz, Hartmann</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomedical optics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rühland, Svenja</au><au>Wechselberger, Alexandra</au><au>Spitzweg, Christine</au><au>Huss, Ralf</au><au>Nelson, Peter J</au><au>Harz, Hartmann</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantification of in vitro mesenchymal stem cell invasion into tumor spheroids using selective plane illumination microscopy</atitle><jtitle>Journal of biomedical optics</jtitle><addtitle>J. Biomed. Opt</addtitle><date>2015-04-01</date><risdate>2015</risdate><volume>20</volume><issue>4</issue><spage>040501</spage><epage>040501</epage><pages>040501-040501</pages><issn>1083-3668</issn><eissn>1560-2281</eissn><abstract>Mesenchymal stem cell (MSC) homing and integration into tumors are under evaluation for clinical application. This approach requires the identification of conditions for optimal tumor invasion. We describe a tool for the in vitro comparison of parameters influencing invasion. Human MSC added to experimental tumor spheroids variably migrates toward the center of the structure. To determine MSC distribution inside the three-dimensional specimen, spatial analysis was performed using selective plane illumination microscopy. 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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Carcinoma, Hepatocellular - pathology Cell Line, Tumor Cell Movement Cell Tracking - methods Humans Lighting - methods Mesenchymal Stromal Cells - pathology Microscopy - methods Neoplasm Invasiveness Spheroids, Cellular - pathology |
| title | Quantification of in vitro mesenchymal stem cell invasion into tumor spheroids using selective plane illumination microscopy |
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