Structure-assisted ligand-binding analysis using fluorogenic photoaffinity labeling

Photoaffinity labeling (PAL) technique using a fluorogenic cross-linker is used to monitor the nucleotide-binding pocket within a protein. A coumarin fluorophore formed in the binding domain due to ultraviolet (UV) irradiation has been shown to accelerate the sequencing of the labeled peptide as wel...

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Veröffentlicht in:	Bioorganic & medicinal chemistry letters 2015-04, Vol.25 (8), p.1675-1678
Hauptverfasser:	Masuda, Souta, Tomohiro, Takenori, Yamaguchi, Shouta, Morimoto, Shota, Hatanaka, Yasumaru
Format:	Artikel
Sprache:	eng
Schlagworte:	Allosteric Regulation Chromatography, High Pressure Liquid Coumarins - chemistry Glutamate Dehydrogenase - metabolism Ligands Photoaffinity Labels - chemistry Photoaffinity Labels - metabolism Substrate Specificity Tandem Mass Spectrometry Ultraviolet Rays
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