The linkage of (1–3)‐β‐glucan to chitin during cell wall assembly in Saccharomyces cerevisiae
Pulse‐chase experiments with [14C]glucose demonstrated that in the cell wall of wild‐type Saccharomyces cerevisiae alkali‐soluble (1–3)‐β‐glucan serves as a precursor for alkali‐insoluble (1–3)‐β‐glucan. The following observations support the notion that the insolubilization of the glucan is caused...
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| Veröffentlicht in: | Yeast (Chichester, England) England), 1994-12, Vol.10 (12), p.1591-1599 |
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| container_title | Yeast (Chichester, England) |
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| creator | Hartland, Robbert P. Vermeulen, Cees A. Sietsma, Johannes H. Wessels, Joseph G. H. Klis, Frans M. |
| description | Pulse‐chase experiments with [14C]glucose demonstrated that in the cell wall of wild‐type Saccharomyces cerevisiae alkali‐soluble (1–3)‐β‐glucan serves as a precursor for alkali‐insoluble (1–3)‐β‐glucan. The following observations support the notion that the insolubilization of the glucan is caused by linkage to chitin: (i) degradation of chitin by chitinase completely dissolved the glucan, and (ii) disruption of the gene for chitin synthase 3 prevented the formation of alkali‐insoluble glucan. These cells, unable to form a glucan–chitin complex, were highly vulnerable to hypo‐osmotic shock indicating that the linkage of the two polymers significantly contributes to the mechanical strength of the cell wall.
Conversion of alkali‐soluble glucan into alkali‐insoluble glucan occurred both early and late during budding and also in the ts‐mutant cdc24‐1 in the absence of bud formation. |
| doi_str_mv | 10.1002/yea.320101208 |
| format | Article |
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Conversion of alkali‐soluble glucan into alkali‐insoluble glucan occurred both early and late during budding and also in the ts‐mutant cdc24‐1 in the absence of bud formation.</description><identifier>ISSN: 0749-503X</identifier><identifier>EISSN: 1097-0061</identifier><identifier>DOI: 10.1002/yea.320101208</identifier><identifier>PMID: 7725794</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>beta-Glucans ; Cell Wall - chemistry ; cell wall synthesis ; chitin ; Chitin - analysis ; glucan ; Glucans - analysis ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - chemistry</subject><ispartof>Yeast (Chichester, England), 1994-12, Vol.10 (12), p.1591-1599</ispartof><rights>Copyright © 1994 John Wiley & Sons Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3368-e3d404ef4c65bc4327d18aea629aa4a3cd85033f7bb1ba20792140f4983f2ec13</citedby><cites>FETCH-LOGICAL-c3368-e3d404ef4c65bc4327d18aea629aa4a3cd85033f7bb1ba20792140f4983f2ec13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fyea.320101208$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fyea.320101208$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7725794$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hartland, Robbert P.</creatorcontrib><creatorcontrib>Vermeulen, Cees A.</creatorcontrib><creatorcontrib>Sietsma, Johannes H.</creatorcontrib><creatorcontrib>Wessels, Joseph G. H.</creatorcontrib><creatorcontrib>Klis, Frans M.</creatorcontrib><title>The linkage of (1–3)‐β‐glucan to chitin during cell wall assembly in Saccharomyces cerevisiae</title><title>Yeast (Chichester, England)</title><addtitle>Yeast</addtitle><description>Pulse‐chase experiments with [14C]glucose demonstrated that in the cell wall of wild‐type Saccharomyces cerevisiae alkali‐soluble (1–3)‐β‐glucan serves as a precursor for alkali‐insoluble (1–3)‐β‐glucan. The following observations support the notion that the insolubilization of the glucan is caused by linkage to chitin: (i) degradation of chitin by chitinase completely dissolved the glucan, and (ii) disruption of the gene for chitin synthase 3 prevented the formation of alkali‐insoluble glucan. These cells, unable to form a glucan–chitin complex, were highly vulnerable to hypo‐osmotic shock indicating that the linkage of the two polymers significantly contributes to the mechanical strength of the cell wall.
Conversion of alkali‐soluble glucan into alkali‐insoluble glucan occurred both early and late during budding and also in the ts‐mutant cdc24‐1 in the absence of bud formation.</description><subject>beta-Glucans</subject><subject>Cell Wall - chemistry</subject><subject>cell wall synthesis</subject><subject>chitin</subject><subject>Chitin - analysis</subject><subject>glucan</subject><subject>Glucans - analysis</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - chemistry</subject><issn>0749-503X</issn><issn>1097-0061</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtO3EAQhlsoERlIliwj9SqChUlVd9ttLxHiEQkpixApWVnldnmmEz-IewzyjiMg5SY5CIfgJGk0I7LLpmrxf_pV9QlxgHCMAOrjzHSsFSCggnxHLBAKmwBk-EoswJoiSUF_eyP2QvgBgJiqfFfsWqtSW5iFqK9XLFvf_6Qly6GRh_h0_1sfPd0_PP6JY9lOjnq5HqRb-bXvZT2Nvl9Kx20r7ygOCoG7qp1lDL-Qcysah252HCIz8q0PnviteN1QG_jddu-Lr-dn16eXydXni0-nJ1eJ0zrLE9a1AcONcVlaOaOVrTEnpkwVRIa0q_P4i25sVWFFCmyh0EBjilw3ih3qffFh03szDr8mDuuy8-H5VOp5mEKJmUXEPI1gsgHdOIQwclPejL6jcS4RymerZbRavliN_Ptt8VR1XL_QW40xt5v8zrc8_7-s_H528q_5LwklhiE</recordid><startdate>199412</startdate><enddate>199412</enddate><creator>Hartland, Robbert P.</creator><creator>Vermeulen, Cees A.</creator><creator>Sietsma, Johannes H.</creator><creator>Wessels, Joseph G. H.</creator><creator>Klis, Frans M.</creator><general>John Wiley & Sons, Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope></search><sort><creationdate>199412</creationdate><title>The linkage of (1–3)‐β‐glucan to chitin during cell wall assembly in Saccharomyces cerevisiae</title><author>Hartland, Robbert P. ; Vermeulen, Cees A. ; Sietsma, Johannes H. ; Wessels, Joseph G. H. ; Klis, Frans M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3368-e3d404ef4c65bc4327d18aea629aa4a3cd85033f7bb1ba20792140f4983f2ec13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>beta-Glucans</topic><topic>Cell Wall - chemistry</topic><topic>cell wall synthesis</topic><topic>chitin</topic><topic>Chitin - analysis</topic><topic>glucan</topic><topic>Glucans - analysis</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hartland, Robbert P.</creatorcontrib><creatorcontrib>Vermeulen, Cees A.</creatorcontrib><creatorcontrib>Sietsma, Johannes H.</creatorcontrib><creatorcontrib>Wessels, Joseph G. H.</creatorcontrib><creatorcontrib>Klis, Frans M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Yeast (Chichester, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hartland, Robbert P.</au><au>Vermeulen, Cees A.</au><au>Sietsma, Johannes H.</au><au>Wessels, Joseph G. H.</au><au>Klis, Frans M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The linkage of (1–3)‐β‐glucan to chitin during cell wall assembly in Saccharomyces cerevisiae</atitle><jtitle>Yeast (Chichester, England)</jtitle><addtitle>Yeast</addtitle><date>1994-12</date><risdate>1994</risdate><volume>10</volume><issue>12</issue><spage>1591</spage><epage>1599</epage><pages>1591-1599</pages><issn>0749-503X</issn><eissn>1097-0061</eissn><abstract>Pulse‐chase experiments with [14C]glucose demonstrated that in the cell wall of wild‐type Saccharomyces cerevisiae alkali‐soluble (1–3)‐β‐glucan serves as a precursor for alkali‐insoluble (1–3)‐β‐glucan. The following observations support the notion that the insolubilization of the glucan is caused by linkage to chitin: (i) degradation of chitin by chitinase completely dissolved the glucan, and (ii) disruption of the gene for chitin synthase 3 prevented the formation of alkali‐insoluble glucan. These cells, unable to form a glucan–chitin complex, were highly vulnerable to hypo‐osmotic shock indicating that the linkage of the two polymers significantly contributes to the mechanical strength of the cell wall.
Conversion of alkali‐soluble glucan into alkali‐insoluble glucan occurred both early and late during budding and also in the ts‐mutant cdc24‐1 in the absence of bud formation.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>7725794</pmid><doi>10.1002/yea.320101208</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0749-503X |
| ispartof | Yeast (Chichester, England), 1994-12, Vol.10 (12), p.1591-1599 |
| issn | 0749-503X 1097-0061 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16711185 |
| source | MEDLINE; Access via Wiley Online Library |
| subjects | beta-Glucans Cell Wall - chemistry cell wall synthesis chitin Chitin - analysis glucan Glucans - analysis Saccharomyces cerevisiae Saccharomyces cerevisiae - chemistry |
| title | The linkage of (1–3)‐β‐glucan to chitin during cell wall assembly in Saccharomyces cerevisiae |
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