Multiplication of Clostridium botulinum in dead honey-bees and bee pupae, a likely source of heavy contamination of honey
Multiplication of Clostridium botulinum in honey-bees was examined to explain the heavy contamination of honey which may occur with this pathogen. When dead bees were inoculated with C. botulinum spores at levels of 10 2−10 3 and incubated aerobically for 10 days, the organisms increased to 10 4−10...
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| Veröffentlicht in: | International journal of food microbiology 1994-02, Vol.21 (3), p.247-252 |
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| container_title | International journal of food microbiology |
| container_volume | 21 |
| creator | Nakano, Hiroyuki Kizaki, Hideki Sakaguchi, Genji |
| description | Multiplication of
Clostridium botulinum in honey-bees was examined to explain the heavy contamination of honey which may occur with this pathogen. When dead bees were inoculated with
C. botulinum spores at levels of 10
2−10
3 and incubated aerobically for 10 days, the organisms increased to 10
4−10
5. When botulinum spores were inoculated together with
Bacillus alvei, the growth of most strains was significantly enhanced (10
5−10
7). Similar results were obtained in bee pupae, but not in bee larvae. The heavy contamination of honey with botulinum spores that we have sometimes encountered may have been caused by contamination from dead bees in which
C. botulinum had proliferated. |
| doi_str_mv | 10.1016/0168-1605(94)90031-0 |
| format | Article |
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Clostridium botulinum in honey-bees was examined to explain the heavy contamination of honey which may occur with this pathogen. When dead bees were inoculated with
C. botulinum spores at levels of 10
2−10
3 and incubated aerobically for 10 days, the organisms increased to 10
4−10
5. When botulinum spores were inoculated together with
Bacillus alvei, the growth of most strains was significantly enhanced (10
5−10
7). Similar results were obtained in bee pupae, but not in bee larvae. The heavy contamination of honey with botulinum spores that we have sometimes encountered may have been caused by contamination from dead bees in which
C. botulinum had proliferated.</description><identifier>ISSN: 0168-1605</identifier><identifier>EISSN: 1879-3460</identifier><identifier>DOI: 10.1016/0168-1605(94)90031-0</identifier><identifier>PMID: 8024976</identifier><identifier>CODEN: IJFMDD</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>ABEILLE DOMESTIQUE ; ABEJA MELIFERA ; Animals ; Apidae ; Apis mellifera ; BACILLUS ; Bacillus alvei ; Bees - microbiology ; Biological and medical sciences ; BOTULISM ; BOTULISME ; BOTULISMO ; CLOSTRIDIUM BOTULINUM ; Clostridium botulinum - growth & development ; Colony Count, Microbial ; Confectionery products and chocolate industries, honey ; CONTAMINACION ; CONTAMINATION ; Food industries ; Food Microbiology ; Fundamental and applied biological sciences. Psychology ; HONEY ; Honey - microbiology ; Honey-bee ; HONEYBEES ; Hymenoptera ; MIEL ; Multiplication ; Pupa - microbiology ; Spores, Bacterial - growth & development</subject><ispartof>International journal of food microbiology, 1994-02, Vol.21 (3), p.247-252</ispartof><rights>1994</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c351t-e7e4fda0a597e7b366d270793219f8a18b6a72aee528acb5fd76fed6dba5403a3</citedby><cites>FETCH-LOGICAL-c351t-e7e4fda0a597e7b366d270793219f8a18b6a72aee528acb5fd76fed6dba5403a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0168-1605(94)90031-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4009462$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8024976$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nakano, Hiroyuki</creatorcontrib><creatorcontrib>Kizaki, Hideki</creatorcontrib><creatorcontrib>Sakaguchi, Genji</creatorcontrib><title>Multiplication of Clostridium botulinum in dead honey-bees and bee pupae, a likely source of heavy contamination of honey</title><title>International journal of food microbiology</title><addtitle>Int J Food Microbiol</addtitle><description>Multiplication of
Clostridium botulinum in honey-bees was examined to explain the heavy contamination of honey which may occur with this pathogen. When dead bees were inoculated with
C. botulinum spores at levels of 10
2−10
3 and incubated aerobically for 10 days, the organisms increased to 10
4−10
5. When botulinum spores were inoculated together with
Bacillus alvei, the growth of most strains was significantly enhanced (10
5−10
7). Similar results were obtained in bee pupae, but not in bee larvae. The heavy contamination of honey with botulinum spores that we have sometimes encountered may have been caused by contamination from dead bees in which
C. botulinum had proliferated.</description><subject>ABEILLE DOMESTIQUE</subject><subject>ABEJA MELIFERA</subject><subject>Animals</subject><subject>Apidae</subject><subject>Apis mellifera</subject><subject>BACILLUS</subject><subject>Bacillus alvei</subject><subject>Bees - microbiology</subject><subject>Biological and medical sciences</subject><subject>BOTULISM</subject><subject>BOTULISME</subject><subject>BOTULISMO</subject><subject>CLOSTRIDIUM BOTULINUM</subject><subject>Clostridium botulinum - growth & development</subject><subject>Colony Count, Microbial</subject><subject>Confectionery products and chocolate industries, honey</subject><subject>CONTAMINACION</subject><subject>CONTAMINATION</subject><subject>Food industries</subject><subject>Food Microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HONEY</subject><subject>Honey - microbiology</subject><subject>Honey-bee</subject><subject>HONEYBEES</subject><subject>Hymenoptera</subject><subject>MIEL</subject><subject>Multiplication</subject><subject>Pupa - microbiology</subject><subject>Spores, Bacterial - growth & development</subject><issn>0168-1605</issn><issn>1879-3460</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kV2L1DAUhoMo6-zqHxCFXIgoWE2aNG1uBBn8glFv9DqcJqdutJN0k3ah_34zO8NcehESeJ_zJjwh5Dln7zjj6n1ZXcUVa15r-UYzJnjFHpAN71pdCanYQ7I5I4_JZc5_GWONEOyCXHSslrpVG7J-X8bZT6O3MPsYaBzodox5Tt75ZU_7OC-jD-XkA3UIjl7HgGvVI2YKwdFyoNMyAb6lQEf_D8eV5rgki4eqa4TbldoYZtj7cL7hvuMJeTTAmPHpab8ivz9_-rX9Wu1-fvm2_birrGj4XGGLcnDAoNEttr1QytUta7WouR464F2voK0Bsak7sH0zuFYN6JTroZFMgLgir469U4o3C-bZ7H22OI4QMC7ZcFXqlOAFlEfQpphzwsFMye8hrYYzczBuDjrNQafR0twbN6yMvTj1L_0e3XnopLjkL085ZAvjkCBYn8-YZExLVRfs2REbIBr4kwryY6clq8uPlfDDMcQi6tZjMtl6DBadT2hn46L__yPvAD6qpmM</recordid><startdate>199402</startdate><enddate>199402</enddate><creator>Nakano, Hiroyuki</creator><creator>Kizaki, Hideki</creator><creator>Sakaguchi, Genji</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>199402</creationdate><title>Multiplication of Clostridium botulinum in dead honey-bees and bee pupae, a likely source of heavy contamination of honey</title><author>Nakano, Hiroyuki ; Kizaki, Hideki ; Sakaguchi, Genji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c351t-e7e4fda0a597e7b366d270793219f8a18b6a72aee528acb5fd76fed6dba5403a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>ABEILLE DOMESTIQUE</topic><topic>ABEJA MELIFERA</topic><topic>Animals</topic><topic>Apidae</topic><topic>Apis mellifera</topic><topic>BACILLUS</topic><topic>Bacillus alvei</topic><topic>Bees - microbiology</topic><topic>Biological and medical sciences</topic><topic>BOTULISM</topic><topic>BOTULISME</topic><topic>BOTULISMO</topic><topic>CLOSTRIDIUM BOTULINUM</topic><topic>Clostridium botulinum - growth & development</topic><topic>Colony Count, Microbial</topic><topic>Confectionery products and chocolate industries, honey</topic><topic>CONTAMINACION</topic><topic>CONTAMINATION</topic><topic>Food industries</topic><topic>Food Microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HONEY</topic><topic>Honey - microbiology</topic><topic>Honey-bee</topic><topic>HONEYBEES</topic><topic>Hymenoptera</topic><topic>MIEL</topic><topic>Multiplication</topic><topic>Pupa - microbiology</topic><topic>Spores, Bacterial - growth & development</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakano, Hiroyuki</creatorcontrib><creatorcontrib>Kizaki, Hideki</creatorcontrib><creatorcontrib>Sakaguchi, Genji</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakano, Hiroyuki</au><au>Kizaki, Hideki</au><au>Sakaguchi, Genji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multiplication of Clostridium botulinum in dead honey-bees and bee pupae, a likely source of heavy contamination of honey</atitle><jtitle>International journal of food microbiology</jtitle><addtitle>Int J Food Microbiol</addtitle><date>1994-02</date><risdate>1994</risdate><volume>21</volume><issue>3</issue><spage>247</spage><epage>252</epage><pages>247-252</pages><issn>0168-1605</issn><eissn>1879-3460</eissn><coden>IJFMDD</coden><abstract>Multiplication of
Clostridium botulinum in honey-bees was examined to explain the heavy contamination of honey which may occur with this pathogen. When dead bees were inoculated with
C. botulinum spores at levels of 10
2−10
3 and incubated aerobically for 10 days, the organisms increased to 10
4−10
5. When botulinum spores were inoculated together with
Bacillus alvei, the growth of most strains was significantly enhanced (10
5−10
7). Similar results were obtained in bee pupae, but not in bee larvae. The heavy contamination of honey with botulinum spores that we have sometimes encountered may have been caused by contamination from dead bees in which
C. botulinum had proliferated.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>8024976</pmid><doi>10.1016/0168-1605(94)90031-0</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-1605 |
| ispartof | International journal of food microbiology, 1994-02, Vol.21 (3), p.247-252 |
| issn | 0168-1605 1879-3460 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16707631 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | ABEILLE DOMESTIQUE ABEJA MELIFERA Animals Apidae Apis mellifera BACILLUS Bacillus alvei Bees - microbiology Biological and medical sciences BOTULISM BOTULISME BOTULISMO CLOSTRIDIUM BOTULINUM Clostridium botulinum - growth & development Colony Count, Microbial Confectionery products and chocolate industries, honey CONTAMINACION CONTAMINATION Food industries Food Microbiology Fundamental and applied biological sciences. Psychology HONEY Honey - microbiology Honey-bee HONEYBEES Hymenoptera MIEL Multiplication Pupa - microbiology Spores, Bacterial - growth & development |
| title | Multiplication of Clostridium botulinum in dead honey-bees and bee pupae, a likely source of heavy contamination of honey |
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