Monitoring biomass and glycerol in an Escherichia coli fermentation using near-infrared spectroscopy
Near-infrared spectroscopy was used to determine biomass and glycerol concentrations in E. coli whole broth fermentation samples. For dry cell weight, a standard error of prediction (SEP) of 0.2 g/L and correlation coefficient (r) of 0.991 were obtained. The SEP and r for glycerol, carbon nutrient,...
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| Veröffentlicht in: | Biotechnology techniques 1994-04, Vol.8 (4), p.281-286 |
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| container_title | Biotechnology techniques |
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| creator | MACALONEY, G HALL, J. W ROLLINS, M. J DRAPER, I THOMPSON, B. G MCNEIL, B |
| description | Near-infrared spectroscopy was used to determine biomass and glycerol concentrations in E. coli whole broth fermentation samples. For dry cell weight, a standard error of prediction (SEP) of 0.2 g/L and correlation coefficient (r) of 0.991 were obtained. The SEP and r for glycerol, carbon nutrient, were 0.3 g/L and 0.979, respectively. Off-line analysis was accomplished within 2 minutes of sampling and therefore provides the opportunity to monitor fermentations quickly enough to permit in-process development and troubleshooting. |
| doi_str_mv | 10.1007/BF00155422 |
| format | Article |
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W ; ROLLINS, M. J ; DRAPER, I ; THOMPSON, B. G ; MCNEIL, B</creator><creatorcontrib>MACALONEY, G ; HALL, J. W ; ROLLINS, M. J ; DRAPER, I ; THOMPSON, B. G ; MCNEIL, B</creatorcontrib><description>Near-infrared spectroscopy was used to determine biomass and glycerol concentrations in E. coli whole broth fermentation samples. For dry cell weight, a standard error of prediction (SEP) of 0.2 g/L and correlation coefficient (r) of 0.991 were obtained. The SEP and r for glycerol, carbon nutrient, were 0.3 g/L and 0.979, respectively. 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Off-line analysis was accomplished within 2 minutes of sampling and therefore provides the opportunity to monitor fermentations quickly enough to permit in-process development and troubleshooting.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Escherichia coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbial engineering. 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| ispartof | Biotechnology techniques, 1994-04, Vol.8 (4), p.281-286 |
| issn | 0951-208X 1573-6784 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16707153 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Biological and medical sciences Biotechnology Escherichia coli Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology |
| title | Monitoring biomass and glycerol in an Escherichia coli fermentation using near-infrared spectroscopy |
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