Enzyme-functionalized gold-coated magnetite nanoparticles as novel hybrid nanomaterials: Synthesis, purification and control of enzyme function by low-frequency magnetic field
•We have developed a procedure for the purification of the magnetite gold core–shell nanoparticles.•We show that the enzyme activity decreases upon exposure of the NPs to the LF AC magnetic field.•Activity of selected enzymes can be efficiently manipulated by a remote magnetic field.•We demonstrate...
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| Veröffentlicht in: | Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2015-01, Vol.125, p.104-109 |
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| creator | Majouga, Alexander Sokolsky-Papkov, Marina Kuznetsov, Artem Lebedev, Dmitry Efremova, Maria Beloglazkina, Elena Rudakovskaya, Polina Veselov, Maxim Zyk, Nikolay Golovin, Yuri Klyachko, Natalia Kabanov, Alexander |
| description | •We have developed a procedure for the purification of the magnetite gold core–shell nanoparticles.•We show that the enzyme activity decreases upon exposure of the NPs to the LF AC magnetic field.•Activity of selected enzymes can be efficiently manipulated by a remote magnetic field.•We demonstrate the effect of the spacer between NPs and enzyme on the kinetics of the enzyme reaction.
The possibility of remotely inducing a defined effect on NPs by means of electromagnetic radiation appears attractive. From a practical point of view, this effect opens horizons for remote control of drug release systems, as well as modulation of biochemical functions in cells. Gold-coated magnetite nanoparticles are perfect candidates for such application. Herein, we have successfully synthesized core–shell NPs having magnetite cores and gold shells modified with various sulphur containing ligands and developed a new, simple and robust procedure for the purification of the resulting nanoparticles. The carboxylic groups displayed at the surface of the NPs were utilized for NP conjugation with a model enzyme (ChT). In the present study, we report the effect of the low-frequency AC magnetic field on the catalytic activity of the immobilized ChT. We show that the enzyme activity decreases upon exposure of the NPs to the field. |
| doi_str_mv | 10.1016/j.colsurfb.2014.11.012 |
| format | Article |
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The possibility of remotely inducing a defined effect on NPs by means of electromagnetic radiation appears attractive. From a practical point of view, this effect opens horizons for remote control of drug release systems, as well as modulation of biochemical functions in cells. Gold-coated magnetite nanoparticles are perfect candidates for such application. Herein, we have successfully synthesized core–shell NPs having magnetite cores and gold shells modified with various sulphur containing ligands and developed a new, simple and robust procedure for the purification of the resulting nanoparticles. The carboxylic groups displayed at the surface of the NPs were utilized for NP conjugation with a model enzyme (ChT). In the present study, we report the effect of the low-frequency AC magnetic field on the catalytic activity of the immobilized ChT. We show that the enzyme activity decreases upon exposure of the NPs to the field.</description><identifier>ISSN: 0927-7765</identifier><identifier>EISSN: 1873-4367</identifier><identifier>DOI: 10.1016/j.colsurfb.2014.11.012</identifier><identifier>PMID: 25460600</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Chromatography, Gel ; Chymotrypsin - chemistry ; Citric Acid - chemistry ; Coated Materials, Biocompatible - chemistry ; Cysteine - chemistry ; Drug delivery systems ; Enzyme Assays ; Enzyme catalytic activity inhibition ; Enzyme immobilization ; Enzymes ; Enzymes, Immobilized - chemistry ; Ferrosoferric Oxide - chemistry ; Gold - chemistry ; Gold-coated magnetite nanoparticles ; Kinetics ; Magnetic Fields ; Magnetite ; Magnetite Nanoparticles - chemistry ; Magnetite Nanoparticles - ultrastructure ; Mathematical models ; Nanoparticles ; Purification ; Sulfur ; Super low-frequency non-heating magnetic field ; Surface Properties</subject><ispartof>Colloids and surfaces, B, Biointerfaces, 2015-01, Vol.125, p.104-109</ispartof><rights>2014 Elsevier B.V.</rights><rights>Copyright © 2014 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c500t-41e8f1d5cea396fcb6066ee4dd2f5ce7692b8966b17228d408a48f3f3e26c7fb3</citedby><cites>FETCH-LOGICAL-c500t-41e8f1d5cea396fcb6066ee4dd2f5ce7692b8966b17228d408a48f3f3e26c7fb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.colsurfb.2014.11.012$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25460600$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Majouga, Alexander</creatorcontrib><creatorcontrib>Sokolsky-Papkov, Marina</creatorcontrib><creatorcontrib>Kuznetsov, Artem</creatorcontrib><creatorcontrib>Lebedev, Dmitry</creatorcontrib><creatorcontrib>Efremova, Maria</creatorcontrib><creatorcontrib>Beloglazkina, Elena</creatorcontrib><creatorcontrib>Rudakovskaya, Polina</creatorcontrib><creatorcontrib>Veselov, Maxim</creatorcontrib><creatorcontrib>Zyk, Nikolay</creatorcontrib><creatorcontrib>Golovin, Yuri</creatorcontrib><creatorcontrib>Klyachko, Natalia</creatorcontrib><creatorcontrib>Kabanov, Alexander</creatorcontrib><title>Enzyme-functionalized gold-coated magnetite nanoparticles as novel hybrid nanomaterials: Synthesis, purification and control of enzyme function by low-frequency magnetic field</title><title>Colloids and surfaces, B, Biointerfaces</title><addtitle>Colloids Surf B Biointerfaces</addtitle><description>•We have developed a procedure for the purification of the magnetite gold core–shell nanoparticles.•We show that the enzyme activity decreases upon exposure of the NPs to the LF AC magnetic field.•Activity of selected enzymes can be efficiently manipulated by a remote magnetic field.•We demonstrate the effect of the spacer between NPs and enzyme on the kinetics of the enzyme reaction.
The possibility of remotely inducing a defined effect on NPs by means of electromagnetic radiation appears attractive. From a practical point of view, this effect opens horizons for remote control of drug release systems, as well as modulation of biochemical functions in cells. Gold-coated magnetite nanoparticles are perfect candidates for such application. Herein, we have successfully synthesized core–shell NPs having magnetite cores and gold shells modified with various sulphur containing ligands and developed a new, simple and robust procedure for the purification of the resulting nanoparticles. The carboxylic groups displayed at the surface of the NPs were utilized for NP conjugation with a model enzyme (ChT). In the present study, we report the effect of the low-frequency AC magnetic field on the catalytic activity of the immobilized ChT. We show that the enzyme activity decreases upon exposure of the NPs to the field.</description><subject>Chromatography, Gel</subject><subject>Chymotrypsin - chemistry</subject><subject>Citric Acid - chemistry</subject><subject>Coated Materials, Biocompatible - chemistry</subject><subject>Cysteine - chemistry</subject><subject>Drug delivery systems</subject><subject>Enzyme Assays</subject><subject>Enzyme catalytic activity inhibition</subject><subject>Enzyme immobilization</subject><subject>Enzymes</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Ferrosoferric Oxide - chemistry</subject><subject>Gold - chemistry</subject><subject>Gold-coated magnetite nanoparticles</subject><subject>Kinetics</subject><subject>Magnetic Fields</subject><subject>Magnetite</subject><subject>Magnetite Nanoparticles - chemistry</subject><subject>Magnetite Nanoparticles - ultrastructure</subject><subject>Mathematical models</subject><subject>Nanoparticles</subject><subject>Purification</subject><subject>Sulfur</subject><subject>Super low-frequency non-heating magnetic field</subject><subject>Surface Properties</subject><issn>0927-7765</issn><issn>1873-4367</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNksFuFSEUhidGY2-rr9CwdOGMwDAw40rT1GrSxIW6JgwcWm4YuMJMm-lL-Ypye3vd2hUE_v98cM5fVecENwQT_mHb6OjzkuzYUExYQ0iDCX1RbUgv2pq1XLysNnigohaCdyfVac5bjDFlRLyuTmjHOOYYb6o_l-FhnaC2S9Czi0F59wAG3URvah3VXPaTugkwuxlQUCHuVJqd9pCRyijEO_Dodh2TM4-3U3Ekp3z-iH6sYb6F7PJ7tFuSs06rPQCpYJCOYU7Ro2gRPPLRkY_GFfl4X9sEvxcIej3iNbIOvHlTvbKlPLx9Ws-qX18uf158ra-_X327-Hxd6w7juWYEektMp0G1A7d6LN_lAMwYasuh4AMd-4HzkQhKe8Nwr1hvW9sC5VrYsT2r3h3q7lIsD8mznFzW4L0KEJcsCefDgPtuIM-RsjKjoafPkLKWMC56XqT8INUp5pzAyl1yk0qrJFjuIyC38hgBuY-AJESWCBTj-RNjGScw_2zHmRfBp4MASv_uHCSZtSutBuMS6Fma6P7H-AszXsr3</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Majouga, Alexander</creator><creator>Sokolsky-Papkov, Marina</creator><creator>Kuznetsov, Artem</creator><creator>Lebedev, Dmitry</creator><creator>Efremova, Maria</creator><creator>Beloglazkina, Elena</creator><creator>Rudakovskaya, Polina</creator><creator>Veselov, Maxim</creator><creator>Zyk, Nikolay</creator><creator>Golovin, Yuri</creator><creator>Klyachko, Natalia</creator><creator>Kabanov, Alexander</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20150101</creationdate><title>Enzyme-functionalized gold-coated magnetite nanoparticles as novel hybrid nanomaterials: Synthesis, purification and control of enzyme function by low-frequency magnetic field</title><author>Majouga, Alexander ; Sokolsky-Papkov, Marina ; Kuznetsov, Artem ; Lebedev, Dmitry ; Efremova, Maria ; Beloglazkina, Elena ; Rudakovskaya, Polina ; Veselov, Maxim ; Zyk, Nikolay ; Golovin, Yuri ; Klyachko, Natalia ; Kabanov, Alexander</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c500t-41e8f1d5cea396fcb6066ee4dd2f5ce7692b8966b17228d408a48f3f3e26c7fb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Chromatography, Gel</topic><topic>Chymotrypsin - chemistry</topic><topic>Citric Acid - chemistry</topic><topic>Coated Materials, Biocompatible - chemistry</topic><topic>Cysteine - chemistry</topic><topic>Drug delivery systems</topic><topic>Enzyme Assays</topic><topic>Enzyme catalytic activity inhibition</topic><topic>Enzyme immobilization</topic><topic>Enzymes</topic><topic>Enzymes, Immobilized - chemistry</topic><topic>Ferrosoferric Oxide - chemistry</topic><topic>Gold - chemistry</topic><topic>Gold-coated magnetite nanoparticles</topic><topic>Kinetics</topic><topic>Magnetic Fields</topic><topic>Magnetite</topic><topic>Magnetite Nanoparticles - chemistry</topic><topic>Magnetite Nanoparticles - ultrastructure</topic><topic>Mathematical models</topic><topic>Nanoparticles</topic><topic>Purification</topic><topic>Sulfur</topic><topic>Super low-frequency non-heating magnetic field</topic><topic>Surface Properties</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Majouga, Alexander</creatorcontrib><creatorcontrib>Sokolsky-Papkov, Marina</creatorcontrib><creatorcontrib>Kuznetsov, Artem</creatorcontrib><creatorcontrib>Lebedev, Dmitry</creatorcontrib><creatorcontrib>Efremova, Maria</creatorcontrib><creatorcontrib>Beloglazkina, Elena</creatorcontrib><creatorcontrib>Rudakovskaya, Polina</creatorcontrib><creatorcontrib>Veselov, Maxim</creatorcontrib><creatorcontrib>Zyk, Nikolay</creatorcontrib><creatorcontrib>Golovin, Yuri</creatorcontrib><creatorcontrib>Klyachko, Natalia</creatorcontrib><creatorcontrib>Kabanov, Alexander</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Colloids and surfaces, B, Biointerfaces</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Majouga, Alexander</au><au>Sokolsky-Papkov, Marina</au><au>Kuznetsov, Artem</au><au>Lebedev, Dmitry</au><au>Efremova, Maria</au><au>Beloglazkina, Elena</au><au>Rudakovskaya, Polina</au><au>Veselov, Maxim</au><au>Zyk, Nikolay</au><au>Golovin, Yuri</au><au>Klyachko, Natalia</au><au>Kabanov, Alexander</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzyme-functionalized gold-coated magnetite nanoparticles as novel hybrid nanomaterials: Synthesis, purification and control of enzyme function by low-frequency magnetic field</atitle><jtitle>Colloids and surfaces, B, Biointerfaces</jtitle><addtitle>Colloids Surf B Biointerfaces</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>125</volume><spage>104</spage><epage>109</epage><pages>104-109</pages><issn>0927-7765</issn><eissn>1873-4367</eissn><abstract>•We have developed a procedure for the purification of the magnetite gold core–shell nanoparticles.•We show that the enzyme activity decreases upon exposure of the NPs to the LF AC magnetic field.•Activity of selected enzymes can be efficiently manipulated by a remote magnetic field.•We demonstrate the effect of the spacer between NPs and enzyme on the kinetics of the enzyme reaction.
The possibility of remotely inducing a defined effect on NPs by means of electromagnetic radiation appears attractive. From a practical point of view, this effect opens horizons for remote control of drug release systems, as well as modulation of biochemical functions in cells. Gold-coated magnetite nanoparticles are perfect candidates for such application. Herein, we have successfully synthesized core–shell NPs having magnetite cores and gold shells modified with various sulphur containing ligands and developed a new, simple and robust procedure for the purification of the resulting nanoparticles. The carboxylic groups displayed at the surface of the NPs were utilized for NP conjugation with a model enzyme (ChT). In the present study, we report the effect of the low-frequency AC magnetic field on the catalytic activity of the immobilized ChT. We show that the enzyme activity decreases upon exposure of the NPs to the field.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>25460600</pmid><doi>10.1016/j.colsurfb.2014.11.012</doi><tpages>6</tpages></addata></record> |
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| ispartof | Colloids and surfaces, B, Biointerfaces, 2015-01, Vol.125, p.104-109 |
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| subjects | Chromatography, Gel Chymotrypsin - chemistry Citric Acid - chemistry Coated Materials, Biocompatible - chemistry Cysteine - chemistry Drug delivery systems Enzyme Assays Enzyme catalytic activity inhibition Enzyme immobilization Enzymes Enzymes, Immobilized - chemistry Ferrosoferric Oxide - chemistry Gold - chemistry Gold-coated magnetite nanoparticles Kinetics Magnetic Fields Magnetite Magnetite Nanoparticles - chemistry Magnetite Nanoparticles - ultrastructure Mathematical models Nanoparticles Purification Sulfur Super low-frequency non-heating magnetic field Surface Properties |
| title | Enzyme-functionalized gold-coated magnetite nanoparticles as novel hybrid nanomaterials: Synthesis, purification and control of enzyme function by low-frequency magnetic field |
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