An isotope-labeled chemical derivatization method for the quantitation of short-chain fatty acids in human feces by liquid chromatography–tandem mass spectrometry

[Display omitted] •3-Nitrophenylhydrazine was used to derivatize short-chain fatty acids (SCFAs) for LC-MS/MS.•13C6 analogues were produced for use as isotope-labeled internal standards.•Isotope-labeled standards compensate for ESI matrix effects in LC-MS/MS.•Femtomolar sensitivities and 93–108% qua...

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Veröffentlicht in:	Analytica chimica acta 2015-01, Vol.854, p.86-94
Hauptverfasser:	Han, Jun, Lin, Karen, Sequeira, Carita, Borchers, Christoph H.
Format:	Artikel
Sprache:	eng
Schlagworte:	3-Nitrophenylhydrazine Calibration Chemical derivatization Chromatography Chromatography, Liquid - methods Diets Fatty acids Fatty Acids, Volatile - analysis Feces Feces - chemistry Homeostasis Human Humans Isotope Labeling Limit of Detection Liquids Mathematical analysis Metabolism Reproducibility of Results Short-chain fatty acid Tandem Mass Spectrometry Ultrahigh performance liquid chromatography–multiple-reaction monitoring mass spectrometry
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