Highly sensitive luciferase reporter assay using a potent destabilization sequence of calpain 3

•We identified a potent destabilization sequence from calpain 3 for luciferase reporter assays.•The sequence (referred to as the C9 fragment) consists of 42 amino acid residues.•Half-life of C9-fused luciferase significantly shorter than PEST-fused luciferase.•The sensitivity of the real-time lucife...

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