Analysis of fast protein phosphorylation kinetics in single cells on a microfluidic chip

In the present study, we developed a microfluidic large-scale integration (mLSI) platform for the temporal and chemical control of cell cultures to study fast kinetics of protein phosphorylation. For in situ protein analysis the mLSI chip integrates the Proximity Ligation Assay (PLA). To investigate...

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Veröffentlicht in:	Lab on a chip 2015-01, Vol.15 (3), p.726-734
Hauptverfasser:	Blazek, Matthias, Santisteban, Tomas Silva, Zengerle, Roland, Meier, Matthias
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biotechnology Cells, Cultured Chips Fluidics Glycogen Synthase Kinase 3 - metabolism Glycogen Synthase Kinase 3 beta Humans Insulin-Like Growth Factor I - metabolism Kinases Kinetics Mice Microfluidic Analytical Techniques - instrumentation Microfluidics NIH 3T3 Cells Phosphorylation Platelet-Derived Growth Factor - metabolism Proteins Proto-Oncogene Proteins c-akt - metabolism Signal Transduction Single-Cell Analysis - instrumentation Temporal logic
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