Immobilization of lipase on porous monodisperse chitosan microspheres
Porous monodisperse chitosan microspheres were synthesized for enzyme immobilization. The microspheres were prepared using microchannels and modified with glutaraldehyde. The microspheres had a mean diameter of 495 µm; the polydispersity indices were less than 0.08, and the specific surface area was...
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| Veröffentlicht in: | Biotechnology and applied biochemistry 2015-01, Vol.62 (1), p.101-106 |
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| container_title | Biotechnology and applied biochemistry |
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| creator | Chen, Yang Liu, Junteng Xia, Chunjie Zhao, Chenxi Ren, Zhongqi Zhang, Weidong |
| description | Porous monodisperse chitosan microspheres were synthesized for enzyme immobilization. The microspheres were prepared using microchannels and modified with glutaraldehyde. The microspheres had a mean diameter of 495 µm; the polydispersity indices were less than 0.08, and the specific surface area was between 121 and 173 m2/g. Candida sp. 1619 lipase was selected as a model lipase. Immobilization conditions such as enzyme loading, glutaraldehyde concentration, and immobilization time were optimized. The temperature, pH, and storage stability of the free and immobilized enzymes were also investigated. The immobilized enzyme had broad‐ranging pH and temperature optima as compared with free enzyme. The storage stability of the immobilized enzyme was higher than that of the free enzyme. |
| doi_str_mv | 10.1002/bab.1242 |
| format | Article |
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The microspheres were prepared using microchannels and modified with glutaraldehyde. The microspheres had a mean diameter of 495 µm; the polydispersity indices were less than 0.08, and the specific surface area was between 121 and 173 m2/g. Candida sp. 1619 lipase was selected as a model lipase. Immobilization conditions such as enzyme loading, glutaraldehyde concentration, and immobilization time were optimized. The temperature, pH, and storage stability of the free and immobilized enzymes were also investigated. The immobilized enzyme had broad‐ranging pH and temperature optima as compared with free enzyme. The storage stability of the immobilized enzyme was higher than that of the free enzyme.</description><identifier>ISSN: 0885-4513</identifier><identifier>EISSN: 1470-8744</identifier><identifier>DOI: 10.1002/bab.1242</identifier><identifier>PMID: 24823273</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Biotechnology ; Candida ; Candida - enzymology ; Chitosan ; Chitosan - chemistry ; Enzyme Stability - drug effects ; Enzymes ; Enzymes, Immobilized - chemistry ; Glutaral - pharmacology ; Glutaraldehyde ; Hydrogen-Ion Concentration ; Immobilization ; immobilized enzyme ; Lipase ; Lipase - chemistry ; lipase activity ; Microspheres ; monodisperse ; Porosity ; porous microspheres ; protein loading ; Storage stability ; Temperature</subject><ispartof>Biotechnology and applied biochemistry, 2015-01, Vol.62 (1), p.101-106</ispartof><rights>2014 International Union of Biochemistry and Molecular Biology, Inc.</rights><rights>Copyright © 2015 International Union of Biochemistry and Molecular Biology, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5522-44db497a9d10a0c8699ddb44e9560accb49008470cd5da71c2469112d6844c5e3</citedby><cites>FETCH-LOGICAL-c5522-44db497a9d10a0c8699ddb44e9560accb49008470cd5da71c2469112d6844c5e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbab.1242$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbab.1242$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24823273$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Yang</creatorcontrib><creatorcontrib>Liu, Junteng</creatorcontrib><creatorcontrib>Xia, Chunjie</creatorcontrib><creatorcontrib>Zhao, Chenxi</creatorcontrib><creatorcontrib>Ren, Zhongqi</creatorcontrib><creatorcontrib>Zhang, Weidong</creatorcontrib><title>Immobilization of lipase on porous monodisperse chitosan microspheres</title><title>Biotechnology and applied biochemistry</title><addtitle>Biotechnology and Applied Biochemistry</addtitle><description>Porous monodisperse chitosan microspheres were synthesized for enzyme immobilization. The microspheres were prepared using microchannels and modified with glutaraldehyde. The microspheres had a mean diameter of 495 µm; the polydispersity indices were less than 0.08, and the specific surface area was between 121 and 173 m2/g. Candida sp. 1619 lipase was selected as a model lipase. Immobilization conditions such as enzyme loading, glutaraldehyde concentration, and immobilization time were optimized. The temperature, pH, and storage stability of the free and immobilized enzymes were also investigated. The immobilized enzyme had broad‐ranging pH and temperature optima as compared with free enzyme. The storage stability of the immobilized enzyme was higher than that of the free enzyme.</description><subject>Biotechnology</subject><subject>Candida</subject><subject>Candida - enzymology</subject><subject>Chitosan</subject><subject>Chitosan - chemistry</subject><subject>Enzyme Stability - drug effects</subject><subject>Enzymes</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Glutaral - pharmacology</subject><subject>Glutaraldehyde</subject><subject>Hydrogen-Ion Concentration</subject><subject>Immobilization</subject><subject>immobilized enzyme</subject><subject>Lipase</subject><subject>Lipase - chemistry</subject><subject>lipase activity</subject><subject>Microspheres</subject><subject>monodisperse</subject><subject>Porosity</subject><subject>porous microspheres</subject><subject>protein loading</subject><subject>Storage stability</subject><subject>Temperature</subject><issn>0885-4513</issn><issn>1470-8744</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUlLAzEYhoMoWhfwF8iAFy-j2ZejFpdCVRC3W8hkUozOTMakxeXXm2JVEARPIV8eHr43LwDbCO4jCPFBZap9hCleAgNEBSyloHQZDKCUrKQMkTWwntIjhFAKiVfBGqYSEyzIAByP2jZUvvHvZupDV4RJ0fjeJFfkSx9imKWiDV2ofepdzGP74Kchma5ovY0h9Q8uurQJViamSW5rcW6Am5Pj6-FZOb48HQ0Px6VlDOOS0rqiShhVI2iglVypOk-oU4xDY21-zCvmALZmtRHIYsoVQrjmklLLHNkAe5_ePobnmUtT3fpkXdOYzuVNNeJcSYEEYf9BIVGUSPEPlDGBuYAqo7u_0Mcwi13OPKdyNoUk_xHOfyhFN9F99K2JbxpBPS9M58L0vLCM7iyEs6p19Tf41VAGyk_gxTfu7U-RPjo8WggXvE9T9_rNm_ikuSCC6buLU317e35FCMb6nnwAofSrhw</recordid><startdate>201501</startdate><enddate>201501</enddate><creator>Chen, Yang</creator><creator>Liu, Junteng</creator><creator>Xia, Chunjie</creator><creator>Zhao, Chenxi</creator><creator>Ren, Zhongqi</creator><creator>Zhang, Weidong</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TB</scope><scope>7TK</scope><scope>7U5</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>L7M</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201501</creationdate><title>Immobilization of lipase on porous monodisperse chitosan microspheres</title><author>Chen, Yang ; Liu, Junteng ; Xia, Chunjie ; Zhao, Chenxi ; Ren, Zhongqi ; Zhang, Weidong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5522-44db497a9d10a0c8699ddb44e9560accb49008470cd5da71c2469112d6844c5e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Biotechnology</topic><topic>Candida</topic><topic>Candida - enzymology</topic><topic>Chitosan</topic><topic>Chitosan - chemistry</topic><topic>Enzyme Stability - drug effects</topic><topic>Enzymes</topic><topic>Enzymes, Immobilized - chemistry</topic><topic>Glutaral - pharmacology</topic><topic>Glutaraldehyde</topic><topic>Hydrogen-Ion Concentration</topic><topic>Immobilization</topic><topic>immobilized enzyme</topic><topic>Lipase</topic><topic>Lipase - chemistry</topic><topic>lipase activity</topic><topic>Microspheres</topic><topic>monodisperse</topic><topic>Porosity</topic><topic>porous microspheres</topic><topic>protein loading</topic><topic>Storage stability</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Yang</creatorcontrib><creatorcontrib>Liu, Junteng</creatorcontrib><creatorcontrib>Xia, Chunjie</creatorcontrib><creatorcontrib>Zhao, Chenxi</creatorcontrib><creatorcontrib>Ren, Zhongqi</creatorcontrib><creatorcontrib>Zhang, Weidong</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology and applied biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Yang</au><au>Liu, Junteng</au><au>Xia, Chunjie</au><au>Zhao, Chenxi</au><au>Ren, Zhongqi</au><au>Zhang, Weidong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immobilization of lipase on porous monodisperse chitosan microspheres</atitle><jtitle>Biotechnology and applied biochemistry</jtitle><addtitle>Biotechnology and Applied Biochemistry</addtitle><date>2015-01</date><risdate>2015</risdate><volume>62</volume><issue>1</issue><spage>101</spage><epage>106</epage><pages>101-106</pages><issn>0885-4513</issn><eissn>1470-8744</eissn><abstract>Porous monodisperse chitosan microspheres were synthesized for enzyme immobilization. The microspheres were prepared using microchannels and modified with glutaraldehyde. The microspheres had a mean diameter of 495 µm; the polydispersity indices were less than 0.08, and the specific surface area was between 121 and 173 m2/g. Candida sp. 1619 lipase was selected as a model lipase. Immobilization conditions such as enzyme loading, glutaraldehyde concentration, and immobilization time were optimized. The temperature, pH, and storage stability of the free and immobilized enzymes were also investigated. The immobilized enzyme had broad‐ranging pH and temperature optima as compared with free enzyme. The storage stability of the immobilized enzyme was higher than that of the free enzyme.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>24823273</pmid><doi>10.1002/bab.1242</doi><tpages>6</tpages></addata></record> |
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| subjects | Biotechnology Candida Candida - enzymology Chitosan Chitosan - chemistry Enzyme Stability - drug effects Enzymes Enzymes, Immobilized - chemistry Glutaral - pharmacology Glutaraldehyde Hydrogen-Ion Concentration Immobilization immobilized enzyme Lipase Lipase - chemistry lipase activity Microspheres monodisperse Porosity porous microspheres protein loading Storage stability Temperature |
| title | Immobilization of lipase on porous monodisperse chitosan microspheres |
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