Rapid and sensitive screening and selective quantification of antibiotics in human urine by two-dimensional ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry
A rapid and sensitive method for the screening and selective quantification of antibiotics in urine by two-dimensional ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was developed. This method allowed the injection of 200 μL urine extract. The 200-μL...
Gespeichert in:
| Veröffentlicht in: | Analytical and bioanalytical chemistry 2014-12, Vol.406 (30), p.8049-8058 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 8058 |
|---|---|
| container_issue | 30 |
| container_start_page | 8049 |
| container_title | Analytical and bioanalytical chemistry |
| container_volume | 406 |
| creator | Wang, He-xing Wang, Bin Zhou, Ying Jiang, Qing-wu |
| description | A rapid and sensitive method for the screening and selective quantification of antibiotics in urine by two-dimensional ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was developed. This method allowed the injection of 200 μL urine extract. The 200-μL injection volume used in this method increased the absolute sensitivity for target antibiotics in solvent by an average 13.3 times, with a range from 8.4 to 28.5 times, compared with the 10-μL conventional injection volume. A 96-well solid phase extraction procedure was established to eliminate the contamination on the chromatographic column resulting from the large-volume injection and increase the throughput of sample preparation. Fourteen target antibiotics from six common categories (β-lactams, quinolones, tetracyclines, macrolides, sulfonamides, and chloramphenicols) were selected as model compounds, and a database containing an additional 74 antibiotics was compiled for posttarget screening. The limit of detection of the target antibiotics, defined as a signal-to-noise ratio of 3, ranged from 0.04 to 1.99 ng/mL. The mean interday recoveries ranged between 79.6 and 121.3 %, with a relative standard deviation from 2.9 to 18.3 % at three spiking levels of 20 ng/mL, 50 ng/mL, and 100 ng/mL. This method was successfully applied in 60 real urine samples from schoolchildren aged 8–11 years, and four target antibiotics (azithromycin, sulfadiazine, trimethoprim, and oxytetracycline) and two posttarget antibiotics (sulfadimidine and cefaclor) were found in the urine samples. This method can be used as a large-scale biomonitoring tool for exposure of the human population to antibiotics.
Graphical abstract
The analysis procedure of urinary antibiotics by two-dimensional UPLC-Q/TOF MS |
| doi_str_mv | 10.1007/s00216-014-8197-6 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_1669869847</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A392414054</galeid><sourcerecordid>A392414054</sourcerecordid><originalsourceid>FETCH-LOGICAL-c542t-b02d2cc9120b2f91efe061beeed554e2f5b260a82b117f4198a8e366d24d8e623</originalsourceid><addsrcrecordid>eNqNktFuFCEUhidGY2v1AbwxJN70ZiqwwDKXTaPVpImJ0WvCMIddGgZmgbHZZ_SlZNy1jUaTZiYZ5vD9Pwf4m-Y1wRcE4_W7jDElosWEtZJ061Y8aU6JILKlguOn92NGT5oXOd9iTLgk4nlzQvmKMynXp82PL3pyA9JhQBlCdsV9B5RNAggubI51D-ZXfTfrUJx1RhcXA4oWLf-9i8WZjFxA23nUAc3JBUD9HpW72A5uXHxj0B7NviQ9QbIxVc4A8m4319XNNsVRl7ips9s9MnGePAzozpXtsuaQ5il6QKVatdG21rvNtqBR54zyVHuraihp_7J5ZrXP8Or4PWu-fXj_9epje_P5-tPV5U1rOKOl7TEdqDEdobintiNgAQvSA8DAOQNqeU8F1pL2hKwtI53UElZCDJQNEgRdnTXnB98pxd0MuajRZQPe6wBxzooI0cn6svVjUFkvi9DHuFLJqaAdqejbv9DbOKd6wllRXpsmVHT8gdpoD8oFG-vpm8VUXa46ygjDnFXq4h9UfQYYnYkBrKv1PwTkIDAp5pzAqim5Uae9IlgtsVSHWKoaS7XEUomqeXNseO5HGO4Vv3NYAXoAcp0KG0gPO_q_608IpfEX</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2511712695</pqid></control><display><type>article</type><title>Rapid and sensitive screening and selective quantification of antibiotics in human urine by two-dimensional ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Wang, He-xing ; Wang, Bin ; Zhou, Ying ; Jiang, Qing-wu</creator><creatorcontrib>Wang, He-xing ; Wang, Bin ; Zhou, Ying ; Jiang, Qing-wu</creatorcontrib><description>A rapid and sensitive method for the screening and selective quantification of antibiotics in urine by two-dimensional ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was developed. This method allowed the injection of 200 μL urine extract. The 200-μL injection volume used in this method increased the absolute sensitivity for target antibiotics in solvent by an average 13.3 times, with a range from 8.4 to 28.5 times, compared with the 10-μL conventional injection volume. A 96-well solid phase extraction procedure was established to eliminate the contamination on the chromatographic column resulting from the large-volume injection and increase the throughput of sample preparation. Fourteen target antibiotics from six common categories (β-lactams, quinolones, tetracyclines, macrolides, sulfonamides, and chloramphenicols) were selected as model compounds, and a database containing an additional 74 antibiotics was compiled for posttarget screening. The limit of detection of the target antibiotics, defined as a signal-to-noise ratio of 3, ranged from 0.04 to 1.99 ng/mL. The mean interday recoveries ranged between 79.6 and 121.3 %, with a relative standard deviation from 2.9 to 18.3 % at three spiking levels of 20 ng/mL, 50 ng/mL, and 100 ng/mL. This method was successfully applied in 60 real urine samples from schoolchildren aged 8–11 years, and four target antibiotics (azithromycin, sulfadiazine, trimethoprim, and oxytetracycline) and two posttarget antibiotics (sulfadimidine and cefaclor) were found in the urine samples. This method can be used as a large-scale biomonitoring tool for exposure of the human population to antibiotics.
Graphical abstract
The analysis procedure of urinary antibiotics by two-dimensional UPLC-Q/TOF MS</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-014-8197-6</identifier><identifier>PMID: 25354887</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Analysis ; Analytical Chemistry ; Anti-Bacterial Agents - urine ; Antibiotics ; Azithromycin ; Biochemistry ; Biological monitoring ; Biomonitoring ; Cefaclor ; Characterization and Evaluation of Materials ; Chemical properties ; Chemistry ; Chemistry and Materials Science ; Child ; Chromatography ; Chromatography, High Pressure Liquid - economics ; Chromatography, High Pressure Liquid - instrumentation ; Chromatography, High Pressure Liquid - methods ; Contamination ; Equipment Design ; Extraction procedures ; Food Science ; Human populations ; Human wastes ; Humans ; Injection ; Ions ; Joining ; Laboratory Medicine ; Limit of Detection ; Liquid chromatography ; Mass spectrometry ; Mass Spectrometry - economics ; Mass Spectrometry - instrumentation ; Mass Spectrometry - methods ; Mass spectroscopy ; Monitoring/Environmental Analysis ; Oxytetracycline ; Quadrupoles ; Quinolones ; Sample preparation ; Scientific imaging ; Screening ; Signal to noise ratio ; Solid phases ; Spectroscopy ; Sulfadiazine ; Sulfadimidine ; Sulfonamides ; Target detection ; Tetracyclines ; Time Factors ; Time-of-flight mass spectrometry ; Trimethoprim ; Two dimensional ; Urine ; β-Lactam antibiotics</subject><ispartof>Analytical and bioanalytical chemistry, 2014-12, Vol.406 (30), p.8049-8058</ispartof><rights>Springer-Verlag Berlin Heidelberg 2014</rights><rights>COPYRIGHT 2014 Springer</rights><rights>Springer-Verlag Berlin Heidelberg 2014.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c542t-b02d2cc9120b2f91efe061beeed554e2f5b260a82b117f4198a8e366d24d8e623</citedby><cites>FETCH-LOGICAL-c542t-b02d2cc9120b2f91efe061beeed554e2f5b260a82b117f4198a8e366d24d8e623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-014-8197-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-014-8197-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25354887$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, He-xing</creatorcontrib><creatorcontrib>Wang, Bin</creatorcontrib><creatorcontrib>Zhou, Ying</creatorcontrib><creatorcontrib>Jiang, Qing-wu</creatorcontrib><title>Rapid and sensitive screening and selective quantification of antibiotics in human urine by two-dimensional ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>A rapid and sensitive method for the screening and selective quantification of antibiotics in urine by two-dimensional ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was developed. This method allowed the injection of 200 μL urine extract. The 200-μL injection volume used in this method increased the absolute sensitivity for target antibiotics in solvent by an average 13.3 times, with a range from 8.4 to 28.5 times, compared with the 10-μL conventional injection volume. A 96-well solid phase extraction procedure was established to eliminate the contamination on the chromatographic column resulting from the large-volume injection and increase the throughput of sample preparation. Fourteen target antibiotics from six common categories (β-lactams, quinolones, tetracyclines, macrolides, sulfonamides, and chloramphenicols) were selected as model compounds, and a database containing an additional 74 antibiotics was compiled for posttarget screening. The limit of detection of the target antibiotics, defined as a signal-to-noise ratio of 3, ranged from 0.04 to 1.99 ng/mL. The mean interday recoveries ranged between 79.6 and 121.3 %, with a relative standard deviation from 2.9 to 18.3 % at three spiking levels of 20 ng/mL, 50 ng/mL, and 100 ng/mL. This method was successfully applied in 60 real urine samples from schoolchildren aged 8–11 years, and four target antibiotics (azithromycin, sulfadiazine, trimethoprim, and oxytetracycline) and two posttarget antibiotics (sulfadimidine and cefaclor) were found in the urine samples. This method can be used as a large-scale biomonitoring tool for exposure of the human population to antibiotics.
Graphical abstract
The analysis procedure of urinary antibiotics by two-dimensional UPLC-Q/TOF MS</description><subject>Analysis</subject><subject>Analytical Chemistry</subject><subject>Anti-Bacterial Agents - urine</subject><subject>Antibiotics</subject><subject>Azithromycin</subject><subject>Biochemistry</subject><subject>Biological monitoring</subject><subject>Biomonitoring</subject><subject>Cefaclor</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemical properties</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Child</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid - economics</subject><subject>Chromatography, High Pressure Liquid - instrumentation</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Contamination</subject><subject>Equipment Design</subject><subject>Extraction procedures</subject><subject>Food Science</subject><subject>Human populations</subject><subject>Human wastes</subject><subject>Humans</subject><subject>Injection</subject><subject>Ions</subject><subject>Joining</subject><subject>Laboratory Medicine</subject><subject>Limit of Detection</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Mass Spectrometry - economics</subject><subject>Mass Spectrometry - instrumentation</subject><subject>Mass Spectrometry - methods</subject><subject>Mass spectroscopy</subject><subject>Monitoring/Environmental Analysis</subject><subject>Oxytetracycline</subject><subject>Quadrupoles</subject><subject>Quinolones</subject><subject>Sample preparation</subject><subject>Scientific imaging</subject><subject>Screening</subject><subject>Signal to noise ratio</subject><subject>Solid phases</subject><subject>Spectroscopy</subject><subject>Sulfadiazine</subject><subject>Sulfadimidine</subject><subject>Sulfonamides</subject><subject>Target detection</subject><subject>Tetracyclines</subject><subject>Time Factors</subject><subject>Time-of-flight mass spectrometry</subject><subject>Trimethoprim</subject><subject>Two dimensional</subject><subject>Urine</subject><subject>β-Lactam antibiotics</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNktFuFCEUhidGY2v1AbwxJN70ZiqwwDKXTaPVpImJ0WvCMIddGgZmgbHZZ_SlZNy1jUaTZiYZ5vD9Pwf4m-Y1wRcE4_W7jDElosWEtZJ061Y8aU6JILKlguOn92NGT5oXOd9iTLgk4nlzQvmKMynXp82PL3pyA9JhQBlCdsV9B5RNAggubI51D-ZXfTfrUJx1RhcXA4oWLf-9i8WZjFxA23nUAc3JBUD9HpW72A5uXHxj0B7NviQ9QbIxVc4A8m4319XNNsVRl7ips9s9MnGePAzozpXtsuaQ5il6QKVatdG21rvNtqBR54zyVHuraihp_7J5ZrXP8Or4PWu-fXj_9epje_P5-tPV5U1rOKOl7TEdqDEdobintiNgAQvSA8DAOQNqeU8F1pL2hKwtI53UElZCDJQNEgRdnTXnB98pxd0MuajRZQPe6wBxzooI0cn6svVjUFkvi9DHuFLJqaAdqejbv9DbOKd6wllRXpsmVHT8gdpoD8oFG-vpm8VUXa46ygjDnFXq4h9UfQYYnYkBrKv1PwTkIDAp5pzAqim5Uae9IlgtsVSHWKoaS7XEUomqeXNseO5HGO4Vv3NYAXoAcp0KG0gPO_q_608IpfEX</recordid><startdate>20141201</startdate><enddate>20141201</enddate><creator>Wang, He-xing</creator><creator>Wang, Bin</creator><creator>Zhou, Ying</creator><creator>Jiang, Qing-wu</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>H8G</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KB.</scope><scope>KR7</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>7QH</scope><scope>7TV</scope><scope>7UA</scope></search><sort><creationdate>20141201</creationdate><title>Rapid and sensitive screening and selective quantification of antibiotics in human urine by two-dimensional ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry</title><author>Wang, He-xing ; Wang, Bin ; Zhou, Ying ; Jiang, Qing-wu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c542t-b02d2cc9120b2f91efe061beeed554e2f5b260a82b117f4198a8e366d24d8e623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Analysis</topic><topic>Analytical Chemistry</topic><topic>Anti-Bacterial Agents - urine</topic><topic>Antibiotics</topic><topic>Azithromycin</topic><topic>Biochemistry</topic><topic>Biological monitoring</topic><topic>Biomonitoring</topic><topic>Cefaclor</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemical properties</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Child</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid - economics</topic><topic>Chromatography, High Pressure Liquid - instrumentation</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Contamination</topic><topic>Equipment Design</topic><topic>Extraction procedures</topic><topic>Food Science</topic><topic>Human populations</topic><topic>Human wastes</topic><topic>Humans</topic><topic>Injection</topic><topic>Ions</topic><topic>Joining</topic><topic>Laboratory Medicine</topic><topic>Limit of Detection</topic><topic>Liquid chromatography</topic><topic>Mass spectrometry</topic><topic>Mass Spectrometry - economics</topic><topic>Mass Spectrometry - instrumentation</topic><topic>Mass Spectrometry - methods</topic><topic>Mass spectroscopy</topic><topic>Monitoring/Environmental Analysis</topic><topic>Oxytetracycline</topic><topic>Quadrupoles</topic><topic>Quinolones</topic><topic>Sample preparation</topic><topic>Scientific imaging</topic><topic>Screening</topic><topic>Signal to noise ratio</topic><topic>Solid phases</topic><topic>Spectroscopy</topic><topic>Sulfadiazine</topic><topic>Sulfadimidine</topic><topic>Sulfonamides</topic><topic>Target detection</topic><topic>Tetracyclines</topic><topic>Time Factors</topic><topic>Time-of-flight mass spectrometry</topic><topic>Trimethoprim</topic><topic>Two dimensional</topic><topic>Urine</topic><topic>β-Lactam antibiotics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, He-xing</creatorcontrib><creatorcontrib>Wang, Bin</creatorcontrib><creatorcontrib>Zhou, Ying</creatorcontrib><creatorcontrib>Jiang, Qing-wu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>Aqualine</collection><collection>Pollution Abstracts</collection><collection>Water Resources Abstracts</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, He-xing</au><au>Wang, Bin</au><au>Zhou, Ying</au><au>Jiang, Qing-wu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid and sensitive screening and selective quantification of antibiotics in human urine by two-dimensional ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2014-12-01</date><risdate>2014</risdate><volume>406</volume><issue>30</issue><spage>8049</spage><epage>8058</epage><pages>8049-8058</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>A rapid and sensitive method for the screening and selective quantification of antibiotics in urine by two-dimensional ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was developed. This method allowed the injection of 200 μL urine extract. The 200-μL injection volume used in this method increased the absolute sensitivity for target antibiotics in solvent by an average 13.3 times, with a range from 8.4 to 28.5 times, compared with the 10-μL conventional injection volume. A 96-well solid phase extraction procedure was established to eliminate the contamination on the chromatographic column resulting from the large-volume injection and increase the throughput of sample preparation. Fourteen target antibiotics from six common categories (β-lactams, quinolones, tetracyclines, macrolides, sulfonamides, and chloramphenicols) were selected as model compounds, and a database containing an additional 74 antibiotics was compiled for posttarget screening. The limit of detection of the target antibiotics, defined as a signal-to-noise ratio of 3, ranged from 0.04 to 1.99 ng/mL. The mean interday recoveries ranged between 79.6 and 121.3 %, with a relative standard deviation from 2.9 to 18.3 % at three spiking levels of 20 ng/mL, 50 ng/mL, and 100 ng/mL. This method was successfully applied in 60 real urine samples from schoolchildren aged 8–11 years, and four target antibiotics (azithromycin, sulfadiazine, trimethoprim, and oxytetracycline) and two posttarget antibiotics (sulfadimidine and cefaclor) were found in the urine samples. This method can be used as a large-scale biomonitoring tool for exposure of the human population to antibiotics.
Graphical abstract
The analysis procedure of urinary antibiotics by two-dimensional UPLC-Q/TOF MS</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>25354887</pmid><doi>10.1007/s00216-014-8197-6</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1618-2642 |
| ispartof | Analytical and bioanalytical chemistry, 2014-12, Vol.406 (30), p.8049-8058 |
| issn | 1618-2642 1618-2650 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1669869847 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Analysis Analytical Chemistry Anti-Bacterial Agents - urine Antibiotics Azithromycin Biochemistry Biological monitoring Biomonitoring Cefaclor Characterization and Evaluation of Materials Chemical properties Chemistry Chemistry and Materials Science Child Chromatography Chromatography, High Pressure Liquid - economics Chromatography, High Pressure Liquid - instrumentation Chromatography, High Pressure Liquid - methods Contamination Equipment Design Extraction procedures Food Science Human populations Human wastes Humans Injection Ions Joining Laboratory Medicine Limit of Detection Liquid chromatography Mass spectrometry Mass Spectrometry - economics Mass Spectrometry - instrumentation Mass Spectrometry - methods Mass spectroscopy Monitoring/Environmental Analysis Oxytetracycline Quadrupoles Quinolones Sample preparation Scientific imaging Screening Signal to noise ratio Solid phases Spectroscopy Sulfadiazine Sulfadimidine Sulfonamides Target detection Tetracyclines Time Factors Time-of-flight mass spectrometry Trimethoprim Two dimensional Urine β-Lactam antibiotics |
| title | Rapid and sensitive screening and selective quantification of antibiotics in human urine by two-dimensional ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T23%3A03%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Rapid%20and%20sensitive%20screening%20and%20selective%20quantification%20of%20antibiotics%20in%20human%20urine%20by%20two-dimensional%20ultraperformance%20liquid%20chromatography%20coupled%20with%20quadrupole%20time-of-flight%20mass%20spectrometry&rft.jtitle=Analytical%20and%20bioanalytical%20chemistry&rft.au=Wang,%20He-xing&rft.date=2014-12-01&rft.volume=406&rft.issue=30&rft.spage=8049&rft.epage=8058&rft.pages=8049-8058&rft.issn=1618-2642&rft.eissn=1618-2650&rft_id=info:doi/10.1007/s00216-014-8197-6&rft_dat=%3Cgale_proqu%3EA392414054%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2511712695&rft_id=info:pmid/25354887&rft_galeid=A392414054&rfr_iscdi=true |