A versatile coiled-coil tethering system for the oriented display of ligands on nanocarriers for targeted gene delivery
Abstract Surface modification of non-viral gene delivery nanocarriers may provide advanced features such as receptor targeting, endosomal escape and nuclear import. We here report the design of a versatile and tunable immobilization protocol to functionalize nanocarriers for improved transient gene...
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Veröffentlicht in: | Biomaterials 2013-01, Vol.34 (4), p.1344-1353 |
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description | Abstract Surface modification of non-viral gene delivery nanocarriers may provide advanced features such as receptor targeting, endosomal escape and nuclear import. We here report the design of a versatile and tunable immobilization protocol to functionalize nanocarriers for improved transient gene expression. Our strategy is based on specific interactions occurring between a coil-tagged ligand and a complementary coil-functionalized nanocarrier. As a proof of concept, targeting of DNA/polyethylenimine polyplexes to the epidermal growth factor receptor of A431 cells was investigated. Coiled-coil-mediated oriented tethering of epidermal growth factor triggered a drastic increase of the internalization rate of the targeted polyplexes. To explore the tunability of our platform, surface density of targeting ligand was varied; our results indicated that the internalization rate varied with the ligand-to-polyplex ratio in a “switch mode” fashion. This work prefigures possible avenues for our coiled-coil platform in multiplex functionalization to address transient gene expression bottlenecks in recombinant protein production. |
doi_str_mv | 10.1016/j.biomaterials.2012.10.047 |
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We here report the design of a versatile and tunable immobilization protocol to functionalize nanocarriers for improved transient gene expression. Our strategy is based on specific interactions occurring between a coil-tagged ligand and a complementary coil-functionalized nanocarrier. As a proof of concept, targeting of DNA/polyethylenimine polyplexes to the epidermal growth factor receptor of A431 cells was investigated. Coiled-coil-mediated oriented tethering of epidermal growth factor triggered a drastic increase of the internalization rate of the targeted polyplexes. To explore the tunability of our platform, surface density of targeting ligand was varied; our results indicated that the internalization rate varied with the ligand-to-polyplex ratio in a “switch mode” fashion. This work prefigures possible avenues for our coiled-coil platform in multiplex functionalization to address transient gene expression bottlenecks in recombinant protein production.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2012.10.047</identifier><identifier>PMID: 23137397</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Advanced Basic Science ; Cell Line, Tumor ; Coiling ; Dentistry ; DNA - administration & dosage ; DNA - genetics ; Epidermal growth factor (EGF) ; Gene expression ; Gene Targeting ; Genes ; Humans ; Ligands ; Lung Neoplasms - genetics ; Multiplexing ; Nanocapsules - administration & dosage ; Nanocapsules - chemistry ; Nanostructure ; Non-viral vector ; Oriented tethering ; Platforms ; Polyethyleneimine - chemistry ; Polyethylenimine ; Receptor, Epidermal Growth Factor - genetics ; Receptors ; Targeting ; Tethering ; Transfection ; Transfection - methods</subject><ispartof>Biomaterials, 2013-01, Vol.34 (4), p.1344-1353</ispartof><rights>2012</rights><rights>Crown Copyright © 2012. 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All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c501t-a3c5e87ed2e92ef7af89378e90ec92af1c2da73a88a1f272621642a364e51153</citedby><cites>FETCH-LOGICAL-c501t-a3c5e87ed2e92ef7af89378e90ec92af1c2da73a88a1f272621642a364e51153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biomaterials.2012.10.047$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23137397$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fortier, Charles</creatorcontrib><creatorcontrib>De Crescenzo, Gregory</creatorcontrib><creatorcontrib>Durocher, Yves</creatorcontrib><title>A versatile coiled-coil tethering system for the oriented display of ligands on nanocarriers for targeted gene delivery</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract Surface modification of non-viral gene delivery nanocarriers may provide advanced features such as receptor targeting, endosomal escape and nuclear import. We here report the design of a versatile and tunable immobilization protocol to functionalize nanocarriers for improved transient gene expression. Our strategy is based on specific interactions occurring between a coil-tagged ligand and a complementary coil-functionalized nanocarrier. As a proof of concept, targeting of DNA/polyethylenimine polyplexes to the epidermal growth factor receptor of A431 cells was investigated. Coiled-coil-mediated oriented tethering of epidermal growth factor triggered a drastic increase of the internalization rate of the targeted polyplexes. To explore the tunability of our platform, surface density of targeting ligand was varied; our results indicated that the internalization rate varied with the ligand-to-polyplex ratio in a “switch mode” fashion. This work prefigures possible avenues for our coiled-coil platform in multiplex functionalization to address transient gene expression bottlenecks in recombinant protein production.</description><subject>Advanced Basic Science</subject><subject>Cell Line, Tumor</subject><subject>Coiling</subject><subject>Dentistry</subject><subject>DNA - administration & dosage</subject><subject>DNA - genetics</subject><subject>Epidermal growth factor (EGF)</subject><subject>Gene expression</subject><subject>Gene Targeting</subject><subject>Genes</subject><subject>Humans</subject><subject>Ligands</subject><subject>Lung Neoplasms - genetics</subject><subject>Multiplexing</subject><subject>Nanocapsules - administration & dosage</subject><subject>Nanocapsules - chemistry</subject><subject>Nanostructure</subject><subject>Non-viral vector</subject><subject>Oriented tethering</subject><subject>Platforms</subject><subject>Polyethyleneimine - chemistry</subject><subject>Polyethylenimine</subject><subject>Receptor, Epidermal Growth Factor - genetics</subject><subject>Receptors</subject><subject>Targeting</subject><subject>Tethering</subject><subject>Transfection</subject><subject>Transfection - methods</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUk1v1DAQtRCILoW_gCxOXLJ47CS2OSBV5VOqxIHeLdeeLF4Se7GzrfLvcbQFIS704pFn3ps3mjeEvAK2BQb9m_32JqTJzpiDHcuWM-C1sGWtfEQ2oKRqOs26x2TDoOWN7oGfkWel7Fn9s5Y_JWdcgJBCyw25u6C3mIudw4jUpfr6Zg10xvl7FYg7WpYy40SHlGlN0ZQDxhk99aEcRrvQNNAx7Gz0haZIo43J2VxBuZw4Nu9wxe8wIvU4hiq4PCdPhjo8vriP5-T644fry8_N1ddPXy4vrhrXMZgbK1yHSqLnqDkO0g5KC6lQM3Sa2wEc91YKq5SFgUvec-hbbkXfYgfQiXPy-tT2kNPPI5bZTKE4HEcbMR2Lgb7XqlMd9A-BtqBbxfj_oZwDCM7kOsDbE9TlVErGwRxymGxeDDCzumn25m83zermWqtuVvLLe53jzYT-D_W3fRXw_gTAusLbunJTXHXHoQ8Z3Wx8Cg_TefdPGzeGGJwdf-CCZZ-OOa4cMIUbZr6td7WeFdQmoBUXvwD6TM1n</recordid><startdate>20130101</startdate><enddate>20130101</enddate><creator>Fortier, Charles</creator><creator>De Crescenzo, Gregory</creator><creator>Durocher, Yves</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>F28</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20130101</creationdate><title>A versatile coiled-coil tethering system for the oriented display of ligands on nanocarriers for targeted gene delivery</title><author>Fortier, Charles ; De Crescenzo, Gregory ; Durocher, Yves</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c501t-a3c5e87ed2e92ef7af89378e90ec92af1c2da73a88a1f272621642a364e51153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Advanced Basic Science</topic><topic>Cell Line, Tumor</topic><topic>Coiling</topic><topic>Dentistry</topic><topic>DNA - administration & dosage</topic><topic>DNA - genetics</topic><topic>Epidermal growth factor (EGF)</topic><topic>Gene expression</topic><topic>Gene Targeting</topic><topic>Genes</topic><topic>Humans</topic><topic>Ligands</topic><topic>Lung Neoplasms - genetics</topic><topic>Multiplexing</topic><topic>Nanocapsules - administration & dosage</topic><topic>Nanocapsules - chemistry</topic><topic>Nanostructure</topic><topic>Non-viral vector</topic><topic>Oriented tethering</topic><topic>Platforms</topic><topic>Polyethyleneimine - chemistry</topic><topic>Polyethylenimine</topic><topic>Receptor, Epidermal Growth Factor - genetics</topic><topic>Receptors</topic><topic>Targeting</topic><topic>Tethering</topic><topic>Transfection</topic><topic>Transfection - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fortier, Charles</creatorcontrib><creatorcontrib>De Crescenzo, Gregory</creatorcontrib><creatorcontrib>Durocher, Yves</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fortier, Charles</au><au>De Crescenzo, Gregory</au><au>Durocher, Yves</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A versatile coiled-coil tethering system for the oriented display of ligands on nanocarriers for targeted gene delivery</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2013-01-01</date><risdate>2013</risdate><volume>34</volume><issue>4</issue><spage>1344</spage><epage>1353</epage><pages>1344-1353</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Abstract Surface modification of non-viral gene delivery nanocarriers may provide advanced features such as receptor targeting, endosomal escape and nuclear import. We here report the design of a versatile and tunable immobilization protocol to functionalize nanocarriers for improved transient gene expression. Our strategy is based on specific interactions occurring between a coil-tagged ligand and a complementary coil-functionalized nanocarrier. As a proof of concept, targeting of DNA/polyethylenimine polyplexes to the epidermal growth factor receptor of A431 cells was investigated. Coiled-coil-mediated oriented tethering of epidermal growth factor triggered a drastic increase of the internalization rate of the targeted polyplexes. To explore the tunability of our platform, surface density of targeting ligand was varied; our results indicated that the internalization rate varied with the ligand-to-polyplex ratio in a “switch mode” fashion. This work prefigures possible avenues for our coiled-coil platform in multiplex functionalization to address transient gene expression bottlenecks in recombinant protein production.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>23137397</pmid><doi>10.1016/j.biomaterials.2012.10.047</doi><tpages>10</tpages></addata></record> |
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subjects | Advanced Basic Science Cell Line, Tumor Coiling Dentistry DNA - administration & dosage DNA - genetics Epidermal growth factor (EGF) Gene expression Gene Targeting Genes Humans Ligands Lung Neoplasms - genetics Multiplexing Nanocapsules - administration & dosage Nanocapsules - chemistry Nanostructure Non-viral vector Oriented tethering Platforms Polyethyleneimine - chemistry Polyethylenimine Receptor, Epidermal Growth Factor - genetics Receptors Targeting Tethering Transfection Transfection - methods |
title | A versatile coiled-coil tethering system for the oriented display of ligands on nanocarriers for targeted gene delivery |
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