Local pH tracking in living cells
Continuous and simultaneous 3D single-particle movement and local pH detection in HeLa cells were demonstrated for the first time by combining fluorescent mesoporous silica nanoparticles (FMSNs) and a single-particle tracking (SPT) technique with a precision of ∼10 nm. FMSNs, synthesized by the co-c...
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| Veröffentlicht in: | Nanoscale 2015-01, Vol.7 (9), p.4217-4225 |
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| container_title | Nanoscale |
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| creator | Tsou, Chieh-Jui Hsia, Chih-Hao Chu, Jia-Yin Hung, Yann Chen, Yi-Ping Chien, Fan-Ching Chou, Keng C Chen, Peilin Mou, Chung-Yuan |
| description | Continuous and simultaneous 3D single-particle movement and local pH detection in HeLa cells were demonstrated for the first time by combining fluorescent mesoporous silica nanoparticles (FMSNs) and a single-particle tracking (SPT) technique with a precision of ∼10 nm. FMSNs, synthesized by the co-condensation of both pH-sensitive and reference dyes with a silica/surfactant source, allow long-term reliable ratiometric pH measurements with a precision better than 0.3 pH unit because of their excellent brightness and stability. pH variation in the surrounding area of FMSNs during endocytosis was monitored in real-time. Acidification and low mobility of FMSNs were observed at the early endocytic stage, whereas basification and high mobility of FMSNs were observed at the late stage. Our results indicate that it is possible to monitor local pH changes in the environments surrounding nanoparticles during the cellular uptake process of FMSNs, which provides much needed information for designing an efficient drug delivery nanosystem. |
| doi_str_mv | 10.1039/c4nr06545j |
| format | Article |
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FMSNs, synthesized by the co-condensation of both pH-sensitive and reference dyes with a silica/surfactant source, allow long-term reliable ratiometric pH measurements with a precision better than 0.3 pH unit because of their excellent brightness and stability. pH variation in the surrounding area of FMSNs during endocytosis was monitored in real-time. Acidification and low mobility of FMSNs were observed at the early endocytic stage, whereas basification and high mobility of FMSNs were observed at the late stage. Our results indicate that it is possible to monitor local pH changes in the environments surrounding nanoparticles during the cellular uptake process of FMSNs, which provides much needed information for designing an efficient drug delivery nanosystem.</description><identifier>ISSN: 2040-3364</identifier><identifier>EISSN: 2040-3372</identifier><identifier>DOI: 10.1039/c4nr06545j</identifier><identifier>PMID: 25672786</identifier><language>eng</language><publisher>England</publisher><subject>Acidification ; Endocytosis ; Fluorescence Resonance Energy Transfer ; Fluorescent Dyes - chemistry ; HeLa Cells ; Humans ; Hydrogen-Ion Concentration ; Microscopy, Fluorescence ; Monitors ; Nanoparticles ; Nanoparticles - chemistry ; Nanoparticles - metabolism ; Nanoparticles - ultrastructure ; Nanostructure ; Silicon dioxide ; Silicon Dioxide - chemistry ; Surface-Active Agents - chemistry ; Three dimensional ; Tracking ; Uptakes</subject><ispartof>Nanoscale, 2015-01, Vol.7 (9), p.4217-4225</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-2f2fa9c96ed4bd1078ead9f04c17427b8c05e715e5b7d00951cc8191e9bdfb753</citedby><cites>FETCH-LOGICAL-c423t-2f2fa9c96ed4bd1078ead9f04c17427b8c05e715e5b7d00951cc8191e9bdfb753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25672786$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsou, Chieh-Jui</creatorcontrib><creatorcontrib>Hsia, Chih-Hao</creatorcontrib><creatorcontrib>Chu, Jia-Yin</creatorcontrib><creatorcontrib>Hung, Yann</creatorcontrib><creatorcontrib>Chen, Yi-Ping</creatorcontrib><creatorcontrib>Chien, Fan-Ching</creatorcontrib><creatorcontrib>Chou, Keng C</creatorcontrib><creatorcontrib>Chen, Peilin</creatorcontrib><creatorcontrib>Mou, Chung-Yuan</creatorcontrib><title>Local pH tracking in living cells</title><title>Nanoscale</title><addtitle>Nanoscale</addtitle><description>Continuous and simultaneous 3D single-particle movement and local pH detection in HeLa cells were demonstrated for the first time by combining fluorescent mesoporous silica nanoparticles (FMSNs) and a single-particle tracking (SPT) technique with a precision of ∼10 nm. FMSNs, synthesized by the co-condensation of both pH-sensitive and reference dyes with a silica/surfactant source, allow long-term reliable ratiometric pH measurements with a precision better than 0.3 pH unit because of their excellent brightness and stability. pH variation in the surrounding area of FMSNs during endocytosis was monitored in real-time. Acidification and low mobility of FMSNs were observed at the early endocytic stage, whereas basification and high mobility of FMSNs were observed at the late stage. Our results indicate that it is possible to monitor local pH changes in the environments surrounding nanoparticles during the cellular uptake process of FMSNs, which provides much needed information for designing an efficient drug delivery nanosystem.</description><subject>Acidification</subject><subject>Endocytosis</subject><subject>Fluorescence Resonance Energy Transfer</subject><subject>Fluorescent Dyes - chemistry</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Microscopy, Fluorescence</subject><subject>Monitors</subject><subject>Nanoparticles</subject><subject>Nanoparticles - chemistry</subject><subject>Nanoparticles - metabolism</subject><subject>Nanoparticles - ultrastructure</subject><subject>Nanostructure</subject><subject>Silicon dioxide</subject><subject>Silicon Dioxide - chemistry</subject><subject>Surface-Active Agents - chemistry</subject><subject>Three dimensional</subject><subject>Tracking</subject><subject>Uptakes</subject><issn>2040-3364</issn><issn>2040-3372</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEtLxDAYRYMozji68QdI3YlQ_fJOllLUUYqC6LqkaSod-zJpBf-9U2ectat7F4fL5SB0iuEKA9XXlrUeBGd8tYfmBBjElEqyv-uCzdBRCCsAoamgh2hGuJBEKjFH52lnTR31y2jwxn5U7XtUtVFdfU3NuroOx-igNHVwJ9tcoLe729dkGafP9w_JTRpbRugQk5KURlstXMHyAoNUzhS6BGaxZETmygJ3EnPHc1kAaI6tVVhjp_OizCWnC3Sx2e199zm6MGRNFaYHpnXdGDIshFZMKw3_QLmkWFGi1-jlBrW-C8G7Mut91Rj_nWHIJntZwp5efu09ruGz7e6YN67YoX-66A-2R2fU</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Tsou, Chieh-Jui</creator><creator>Hsia, Chih-Hao</creator><creator>Chu, Jia-Yin</creator><creator>Hung, Yann</creator><creator>Chen, Yi-Ping</creator><creator>Chien, Fan-Ching</creator><creator>Chou, Keng C</creator><creator>Chen, Peilin</creator><creator>Mou, Chung-Yuan</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20150101</creationdate><title>Local pH tracking in living cells</title><author>Tsou, Chieh-Jui ; 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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Acidification Endocytosis Fluorescence Resonance Energy Transfer Fluorescent Dyes - chemistry HeLa Cells Humans Hydrogen-Ion Concentration Microscopy, Fluorescence Monitors Nanoparticles Nanoparticles - chemistry Nanoparticles - metabolism Nanoparticles - ultrastructure Nanostructure Silicon dioxide Silicon Dioxide - chemistry Surface-Active Agents - chemistry Three dimensional Tracking Uptakes |
| title | Local pH tracking in living cells |
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