Improved ethanol tolerance and ethanol production from glycerol in a streptomycin-resistant Klebsiella variicola mutant obtained by ribosome engineering
•Streptomycin-resistant K. variicola TB-83D was obtained by ribosome engineering.•Ethanol tolerance and ethanol production were improved by rpsL mutation.•Ethanol production was increased significantly by addition of YE.•Highest ethanol concentration of 34g/L was obtained by using YE and CSL.•CSL wa...
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| Veröffentlicht in: | Bioresource technology 2015-01, Vol.176, p.156-162 |
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| creator | Suzuki, Toshihiro Seta, Kohei Nishikawa, Chiaki Hara, Eri Shigeno, Toshiya Nakajima-Kambe, Toshiaki |
| description | •Streptomycin-resistant K. variicola TB-83D was obtained by ribosome engineering.•Ethanol tolerance and ethanol production were improved by rpsL mutation.•Ethanol production was increased significantly by addition of YE.•Highest ethanol concentration of 34g/L was obtained by using YE and CSL.•CSL was suitable for reducing by-product production and production costs.
To improve the ethanol tolerance of the Klebsiella variicola strain TB-83, we obtained the streptomycin-resistant, ethanol-tolerant mutant strain TB-83D by a ribosome engineering approach. Strain TB-83D was able to grow in the presence of 7% (v/v) ethanol and it showed higher ethanol production than strain TB-83. Examination of various culture conditions revealed that yeast extract was essential for ethanol production and bacterial growth. In addition, ethanol production was elevated to 32g/L by the addition of yeast extract; however, ethanol production was inhibited by formate accumulation. With regard to cost reduction, the use of corn steep liquor (CSL) markedly decreased the formate concentration, and 34g/L ethanol was produced by combining yeast extract with CSL. Our study is the first to improve ethanol tolerance and productivity by a ribosome engineering approach, and we found that strain TB-83D is effective for ethanol production from glycerol. |
| doi_str_mv | 10.1016/j.biortech.2014.10.153 |
| format | Article |
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To improve the ethanol tolerance of the Klebsiella variicola strain TB-83, we obtained the streptomycin-resistant, ethanol-tolerant mutant strain TB-83D by a ribosome engineering approach. Strain TB-83D was able to grow in the presence of 7% (v/v) ethanol and it showed higher ethanol production than strain TB-83. Examination of various culture conditions revealed that yeast extract was essential for ethanol production and bacterial growth. In addition, ethanol production was elevated to 32g/L by the addition of yeast extract; however, ethanol production was inhibited by formate accumulation. With regard to cost reduction, the use of corn steep liquor (CSL) markedly decreased the formate concentration, and 34g/L ethanol was produced by combining yeast extract with CSL. Our study is the first to improve ethanol tolerance and productivity by a ribosome engineering approach, and we found that strain TB-83D is effective for ethanol production from glycerol.</description><identifier>ISSN: 0960-8524</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2014.10.153</identifier><identifier>PMID: 25460997</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Bacteria ; Batch Cell Culture Techniques ; Biofuels ; Corn ; Culture Media ; Drug Resistance, Bacterial ; Ethanol ; Ethanol - metabolism ; Ethanol production ; Ethanol tolerance ; Ethyl alcohol ; Fermentation ; Formates - metabolism ; Glycerol ; Glycerol - metabolism ; Glycerols ; Klebsiella ; Klebsiella - drug effects ; Klebsiella - genetics ; Klebsiella - metabolism ; Klebsiella variicola ; Mutation ; Protein Engineering ; Ribosome engineering ; Ribosomes - genetics ; Strain ; Streptomycin - pharmacology ; Tolerances ; Yeast</subject><ispartof>Bioresource technology, 2015-01, Vol.176, p.156-162</ispartof><rights>2014 Elsevier Ltd</rights><rights>Copyright © 2014 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c500t-3e6fa110505af68e31ee370870c38399cedf82cdeb1fe14bdd2445f522af76133</citedby><cites>FETCH-LOGICAL-c500t-3e6fa110505af68e31ee370870c38399cedf82cdeb1fe14bdd2445f522af76133</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biortech.2014.10.153$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27926,27927,45997</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25460997$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suzuki, Toshihiro</creatorcontrib><creatorcontrib>Seta, Kohei</creatorcontrib><creatorcontrib>Nishikawa, Chiaki</creatorcontrib><creatorcontrib>Hara, Eri</creatorcontrib><creatorcontrib>Shigeno, Toshiya</creatorcontrib><creatorcontrib>Nakajima-Kambe, Toshiaki</creatorcontrib><title>Improved ethanol tolerance and ethanol production from glycerol in a streptomycin-resistant Klebsiella variicola mutant obtained by ribosome engineering</title><title>Bioresource technology</title><addtitle>Bioresour Technol</addtitle><description>•Streptomycin-resistant K. variicola TB-83D was obtained by ribosome engineering.•Ethanol tolerance and ethanol production were improved by rpsL mutation.•Ethanol production was increased significantly by addition of YE.•Highest ethanol concentration of 34g/L was obtained by using YE and CSL.•CSL was suitable for reducing by-product production and production costs.
To improve the ethanol tolerance of the Klebsiella variicola strain TB-83, we obtained the streptomycin-resistant, ethanol-tolerant mutant strain TB-83D by a ribosome engineering approach. Strain TB-83D was able to grow in the presence of 7% (v/v) ethanol and it showed higher ethanol production than strain TB-83. Examination of various culture conditions revealed that yeast extract was essential for ethanol production and bacterial growth. In addition, ethanol production was elevated to 32g/L by the addition of yeast extract; however, ethanol production was inhibited by formate accumulation. With regard to cost reduction, the use of corn steep liquor (CSL) markedly decreased the formate concentration, and 34g/L ethanol was produced by combining yeast extract with CSL. Our study is the first to improve ethanol tolerance and productivity by a ribosome engineering approach, and we found that strain TB-83D is effective for ethanol production from glycerol.</description><subject>Bacteria</subject><subject>Batch Cell Culture Techniques</subject><subject>Biofuels</subject><subject>Corn</subject><subject>Culture Media</subject><subject>Drug Resistance, Bacterial</subject><subject>Ethanol</subject><subject>Ethanol - metabolism</subject><subject>Ethanol production</subject><subject>Ethanol tolerance</subject><subject>Ethyl alcohol</subject><subject>Fermentation</subject><subject>Formates - metabolism</subject><subject>Glycerol</subject><subject>Glycerol - metabolism</subject><subject>Glycerols</subject><subject>Klebsiella</subject><subject>Klebsiella - drug effects</subject><subject>Klebsiella - genetics</subject><subject>Klebsiella - metabolism</subject><subject>Klebsiella variicola</subject><subject>Mutation</subject><subject>Protein Engineering</subject><subject>Ribosome engineering</subject><subject>Ribosomes - genetics</subject><subject>Strain</subject><subject>Streptomycin - pharmacology</subject><subject>Tolerances</subject><subject>Yeast</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS0EokPhFSov2WTwf5IdqOKnohIbWFuOfTP1KLEH2xlp3qSPi9NpYUlXts797HPvPQhdUbKlhKoP--3gYypg77aMULFddclfoA3tWt6wvlUv0Yb0ijSdZOICvcl5TwjhtGWv0QWTQpG-bzfo_mY-pHgEh6HcmRAnXOIEyQQL2IR_aoXcYouPAY8pzng3nSykWvABG5xLgkOJ88n60CTIPhcTCv4-wZA9TJPBR5O8t7He5uWhFodifKi-wwknP8QcZ8AQdlWD5MPuLXo1minDu8fzEv368vnn9bfm9sfXm-tPt42VhJSGgxoNpUQSaUbVAacAvCVdSyzveN9bcGPHrIOBjkDF4BwTQo6SMTO2inJ-id6f_60T_l4gFz37bNeeA8Qla6pU3wlJiXgOSngneU-fgXLBWqakrKg6ozbFnBOM-pD8bNJJU6LXrPVeP2Wt16wfdLl2fvXosQwzuL_PnsKtwMczAHV_Rw9JZ-uhJut8Alu0i_5_Hn8ATiXB-Q</recordid><startdate>201501</startdate><enddate>201501</enddate><creator>Suzuki, Toshihiro</creator><creator>Seta, Kohei</creator><creator>Nishikawa, Chiaki</creator><creator>Hara, Eri</creator><creator>Shigeno, Toshiya</creator><creator>Nakajima-Kambe, Toshiaki</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7QO</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7SU</scope><scope>7TB</scope><scope>KR7</scope></search><sort><creationdate>201501</creationdate><title>Improved ethanol tolerance and ethanol production from glycerol in a streptomycin-resistant Klebsiella variicola mutant obtained by ribosome engineering</title><author>Suzuki, Toshihiro ; Seta, Kohei ; Nishikawa, Chiaki ; Hara, Eri ; Shigeno, Toshiya ; Nakajima-Kambe, Toshiaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c500t-3e6fa110505af68e31ee370870c38399cedf82cdeb1fe14bdd2445f522af76133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Bacteria</topic><topic>Batch Cell Culture Techniques</topic><topic>Biofuels</topic><topic>Corn</topic><topic>Culture Media</topic><topic>Drug Resistance, Bacterial</topic><topic>Ethanol</topic><topic>Ethanol - metabolism</topic><topic>Ethanol production</topic><topic>Ethanol tolerance</topic><topic>Ethyl alcohol</topic><topic>Fermentation</topic><topic>Formates - metabolism</topic><topic>Glycerol</topic><topic>Glycerol - metabolism</topic><topic>Glycerols</topic><topic>Klebsiella</topic><topic>Klebsiella - drug effects</topic><topic>Klebsiella - genetics</topic><topic>Klebsiella - metabolism</topic><topic>Klebsiella variicola</topic><topic>Mutation</topic><topic>Protein Engineering</topic><topic>Ribosome engineering</topic><topic>Ribosomes - genetics</topic><topic>Strain</topic><topic>Streptomycin - pharmacology</topic><topic>Tolerances</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suzuki, Toshihiro</creatorcontrib><creatorcontrib>Seta, Kohei</creatorcontrib><creatorcontrib>Nishikawa, Chiaki</creatorcontrib><creatorcontrib>Hara, Eri</creatorcontrib><creatorcontrib>Shigeno, Toshiya</creatorcontrib><creatorcontrib>Nakajima-Kambe, Toshiaki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Engineering Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Civil Engineering Abstracts</collection><jtitle>Bioresource technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suzuki, Toshihiro</au><au>Seta, Kohei</au><au>Nishikawa, Chiaki</au><au>Hara, Eri</au><au>Shigeno, Toshiya</au><au>Nakajima-Kambe, Toshiaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved ethanol tolerance and ethanol production from glycerol in a streptomycin-resistant Klebsiella variicola mutant obtained by ribosome engineering</atitle><jtitle>Bioresource technology</jtitle><addtitle>Bioresour Technol</addtitle><date>2015-01</date><risdate>2015</risdate><volume>176</volume><spage>156</spage><epage>162</epage><pages>156-162</pages><issn>0960-8524</issn><eissn>1873-2976</eissn><abstract>•Streptomycin-resistant K. variicola TB-83D was obtained by ribosome engineering.•Ethanol tolerance and ethanol production were improved by rpsL mutation.•Ethanol production was increased significantly by addition of YE.•Highest ethanol concentration of 34g/L was obtained by using YE and CSL.•CSL was suitable for reducing by-product production and production costs.
To improve the ethanol tolerance of the Klebsiella variicola strain TB-83, we obtained the streptomycin-resistant, ethanol-tolerant mutant strain TB-83D by a ribosome engineering approach. Strain TB-83D was able to grow in the presence of 7% (v/v) ethanol and it showed higher ethanol production than strain TB-83. Examination of various culture conditions revealed that yeast extract was essential for ethanol production and bacterial growth. In addition, ethanol production was elevated to 32g/L by the addition of yeast extract; however, ethanol production was inhibited by formate accumulation. With regard to cost reduction, the use of corn steep liquor (CSL) markedly decreased the formate concentration, and 34g/L ethanol was produced by combining yeast extract with CSL. Our study is the first to improve ethanol tolerance and productivity by a ribosome engineering approach, and we found that strain TB-83D is effective for ethanol production from glycerol.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>25460997</pmid><doi>10.1016/j.biortech.2014.10.153</doi><tpages>7</tpages></addata></record> |
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| subjects | Bacteria Batch Cell Culture Techniques Biofuels Corn Culture Media Drug Resistance, Bacterial Ethanol Ethanol - metabolism Ethanol production Ethanol tolerance Ethyl alcohol Fermentation Formates - metabolism Glycerol Glycerol - metabolism Glycerols Klebsiella Klebsiella - drug effects Klebsiella - genetics Klebsiella - metabolism Klebsiella variicola Mutation Protein Engineering Ribosome engineering Ribosomes - genetics Strain Streptomycin - pharmacology Tolerances Yeast |
| title | Improved ethanol tolerance and ethanol production from glycerol in a streptomycin-resistant Klebsiella variicola mutant obtained by ribosome engineering |
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