Structural variation of eucalyptus lignin in a combination of hydrothermal and alkali treatments

[Display omitted] •Hydrothermal and alkali treatments were combined to fractionate lignin.•An increase in pretreatment severity facilitated the release of lignin.•The β-O-4 linkage was cleaved gradually with the increased temperature.•Mw of lignin decreased from 1630 to 510g/mol as temperature incre...

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