Identification of Carriers Among Individuals Recruited in the Typhoid Registry in Malaysia Using Stool Culture, Polymerase Chain Reaction, and Dot Enzyme Immunoassay as Detection Tools
Chronic carriers of Salmonella Typhi act as reservoirs for the organism and become the agents of typhoid outbreaks in a community. In this study, chronic carriers in Kelantan, Malaysia were first identified using the culture and polymerase chain reaction method. Then, a novel serological tool, desig...
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| Veröffentlicht in: | Asia-Pacific journal of public health 2015-03, Vol.27 (2), p.NP2740-NP2748 |
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| container_title | Asia-Pacific journal of public health |
| container_volume | 27 |
| creator | Chua, Ang Lim Aziah, Ismail Balaram, Prabha Bhuvanendran, Saatheeyavaane Anthony, Amy Amilda Mohmad, Siti Norazura Nasir, Norhafiza M. Hassan, Haslizai Naim, Rochman Meran, Lila P. Hussin, Hani M. Ismail, Asma |
| description | Chronic carriers of Salmonella Typhi act as reservoirs for the organism and become the agents of typhoid outbreaks in a community. In this study, chronic carriers in Kelantan, Malaysia were first identified using the culture and polymerase chain reaction method. Then, a novel serological tool, designated Typhidot-C, was evaluated in retrospect using the detected individuals as control positives. Chronic carriage positive by the culture and polymerase chain reaction method was recorded at 3.6% (4 out of 110) among individuals who previously had acute typhoid fever and a 9.4% (10 out of 106) carriage rate was observed among food handlers screened during outbreaks. The Typhidot-C assay was able to detect all these positive carriers showing its potential as a viable carrier screening tool and can be used for efficient detection of typhoid carriers in an endemic area. These findings were used to establish the first carrier registry for S Typhi carriers in Malaysia. |
| doi_str_mv | 10.1177/1010539512458521 |
| format | Article |
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In this study, chronic carriers in Kelantan, Malaysia were first identified using the culture and polymerase chain reaction method. Then, a novel serological tool, designated Typhidot-C, was evaluated in retrospect using the detected individuals as control positives. Chronic carriage positive by the culture and polymerase chain reaction method was recorded at 3.6% (4 out of 110) among individuals who previously had acute typhoid fever and a 9.4% (10 out of 106) carriage rate was observed among food handlers screened during outbreaks. The Typhidot-C assay was able to detect all these positive carriers showing its potential as a viable carrier screening tool and can be used for efficient detection of typhoid carriers in an endemic area. These findings were used to establish the first carrier registry for S Typhi carriers in Malaysia.</description><identifier>ISSN: 1010-5395</identifier><identifier>EISSN: 1941-2479</identifier><identifier>DOI: 10.1177/1010539512458521</identifier><identifier>PMID: 23000800</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Carrier State - epidemiology ; Feces - microbiology ; Food Handling ; Humans ; Immunoenzyme Techniques ; Malaysia - epidemiology ; Polymerase Chain Reaction ; Registries - statistics & numerical data ; Salmonella typhi - isolation & purification ; Typhoid Fever - epidemiology</subject><ispartof>Asia-Pacific journal of public health, 2015-03, Vol.27 (2), p.NP2740-NP2748</ispartof><rights>2012 APJPH</rights><rights>2012 APJPH.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c337t-71cbeb604bf3001cb4dd07b87549a2452fd96692c527796c9819494b8044ff843</citedby><cites>FETCH-LOGICAL-c337t-71cbeb604bf3001cb4dd07b87549a2452fd96692c527796c9819494b8044ff843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/1010539512458521$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/1010539512458521$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>315,782,786,21828,27933,27934,43630,43631</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23000800$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chua, Ang Lim</creatorcontrib><creatorcontrib>Aziah, Ismail</creatorcontrib><creatorcontrib>Balaram, Prabha</creatorcontrib><creatorcontrib>Bhuvanendran, Saatheeyavaane</creatorcontrib><creatorcontrib>Anthony, Amy Amilda</creatorcontrib><creatorcontrib>Mohmad, Siti Norazura</creatorcontrib><creatorcontrib>Nasir, Norhafiza M.</creatorcontrib><creatorcontrib>Hassan, Haslizai</creatorcontrib><creatorcontrib>Naim, Rochman</creatorcontrib><creatorcontrib>Meran, Lila P.</creatorcontrib><creatorcontrib>Hussin, Hani M.</creatorcontrib><creatorcontrib>Ismail, Asma</creatorcontrib><title>Identification of Carriers Among Individuals Recruited in the Typhoid Registry in Malaysia Using Stool Culture, Polymerase Chain Reaction, and Dot Enzyme Immunoassay as Detection Tools</title><title>Asia-Pacific journal of public health</title><addtitle>Asia Pac J Public Health</addtitle><description>Chronic carriers of Salmonella Typhi act as reservoirs for the organism and become the agents of typhoid outbreaks in a community. In this study, chronic carriers in Kelantan, Malaysia were first identified using the culture and polymerase chain reaction method. Then, a novel serological tool, designated Typhidot-C, was evaluated in retrospect using the detected individuals as control positives. Chronic carriage positive by the culture and polymerase chain reaction method was recorded at 3.6% (4 out of 110) among individuals who previously had acute typhoid fever and a 9.4% (10 out of 106) carriage rate was observed among food handlers screened during outbreaks. The Typhidot-C assay was able to detect all these positive carriers showing its potential as a viable carrier screening tool and can be used for efficient detection of typhoid carriers in an endemic area. These findings were used to establish the first carrier registry for S Typhi carriers in Malaysia.</description><subject>Carrier State - epidemiology</subject><subject>Feces - microbiology</subject><subject>Food Handling</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Malaysia - epidemiology</subject><subject>Polymerase Chain Reaction</subject><subject>Registries - statistics & numerical data</subject><subject>Salmonella typhi - isolation & purification</subject><subject>Typhoid Fever - epidemiology</subject><issn>1010-5395</issn><issn>1941-2479</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUtv1DAUhSMEoqWwZ4XukkUDduLE8bJKC4zUqlWZriMnvplxldiDH5XCL-vPq4cpLCqx8uN859j33iz7SMkXSjn_SgklVSkqWrCqqQr6KjumgtG8YFy8Tvsk53v9KHvn_T0hlWiIeJsdFSUhpCHkOHtcKTRBj3qQQVsDdoRWOqfReTibrdnAyij9oFWUk4dbHFzUARVoA2GLsF52W6tVEjbaB7fs76_kJBevJdx5nfw_g7UTtHEK0eEp3NhpmdFJj9BuZcJvUQ77p09BGgXnNsCF-Z0QWM1zNFZ6LxeQHs4x4B8Q1inQv8_ejOlL-OF5Pcnuvl2s2x_55fX3VXt2mQ9lyUPO6dBjXxPWj6nmdGBKEd43vGJCpq4VoxJ1LYqhKjgX9SCa1EDB-oYwNo4NK0-yz4fcnbO_IvrQzdoPOE3SoI2-o8ndMELqIqHkgA7Oeu9w7HZOz9ItHSXdfl7dy3kly6fn9NjPqP4Z_g4oAfkB8HKD3b2NzqRq_x_4BCgJnu8</recordid><startdate>20150301</startdate><enddate>20150301</enddate><creator>Chua, Ang Lim</creator><creator>Aziah, Ismail</creator><creator>Balaram, Prabha</creator><creator>Bhuvanendran, Saatheeyavaane</creator><creator>Anthony, Amy Amilda</creator><creator>Mohmad, Siti Norazura</creator><creator>Nasir, Norhafiza M.</creator><creator>Hassan, Haslizai</creator><creator>Naim, Rochman</creator><creator>Meran, Lila P.</creator><creator>Hussin, Hani M.</creator><creator>Ismail, Asma</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150301</creationdate><title>Identification of Carriers Among Individuals Recruited in the Typhoid Registry in Malaysia Using Stool Culture, Polymerase Chain Reaction, and Dot Enzyme Immunoassay as Detection Tools</title><author>Chua, Ang Lim ; 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| identifier | ISSN: 1010-5395 |
| ispartof | Asia-Pacific journal of public health, 2015-03, Vol.27 (2), p.NP2740-NP2748 |
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| subjects | Carrier State - epidemiology Feces - microbiology Food Handling Humans Immunoenzyme Techniques Malaysia - epidemiology Polymerase Chain Reaction Registries - statistics & numerical data Salmonella typhi - isolation & purification Typhoid Fever - epidemiology |
| title | Identification of Carriers Among Individuals Recruited in the Typhoid Registry in Malaysia Using Stool Culture, Polymerase Chain Reaction, and Dot Enzyme Immunoassay as Detection Tools |
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