Red-Emitting DPSB-Based Conjugated Polymer Nanoparticles with High Two-Photon Brightness for Cell Membrane Imaging
New red-emitting conjugated polymers have been successfully synthesized by incorporating classical two-photon absorption (TPA) units, electron-rich units, and a small amount of electron-deficient units along the polymer backbones. Water-dispersible nanoparticles (NPs) based on these polymers were al...
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| Veröffentlicht in: | ACS applied materials & interfaces 2015-04, Vol.7 (12), p.6754-6763 |
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| creator | Liu, Peng Li, Shuang Jin, Yaocheng Qian, Linghui Gao, Nengyue Yao, Shao Q Huang, Fei Xu, Qing-Hua Cao, Yong |
| description | New red-emitting conjugated polymers have been successfully synthesized by incorporating classical two-photon absorption (TPA) units, electron-rich units, and a small amount of electron-deficient units along the polymer backbones. Water-dispersible nanoparticles (NPs) based on these polymers were also fabricated for applications in two-photon excitation fluorescence imaging of cell membrane. Through optimization of the polymer/matrix mass ratio and the initial feed concentration of the polymer solution, a high quantum yield (QY) of 24% was achieved for the red-emitting NPs in water. TPA cross section and two-photon action cross section values of these polymers at 750 nm reached up to 1000 GM and 190 GM per repeat unit in aqueous media, 2.5 × 105 GM and 4.7 × 104 GM per NP, respectively. Furthermore, these NPs displayed excellent photostability and biocompatibility. Their applications as two-photon excitation fluorescence probes for cell membrane imaging have been demonstrated in three different cell lines with excellent imaging contrast. These results demonstrated that these polymer NPs hold great potentials as excellent two-photon excitation fluorescence probes in various biological applications. |
| doi_str_mv | 10.1021/acsami.5b00223 |
| format | Article |
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Water-dispersible nanoparticles (NPs) based on these polymers were also fabricated for applications in two-photon excitation fluorescence imaging of cell membrane. Through optimization of the polymer/matrix mass ratio and the initial feed concentration of the polymer solution, a high quantum yield (QY) of 24% was achieved for the red-emitting NPs in water. TPA cross section and two-photon action cross section values of these polymers at 750 nm reached up to 1000 GM and 190 GM per repeat unit in aqueous media, 2.5 × 105 GM and 4.7 × 104 GM per NP, respectively. Furthermore, these NPs displayed excellent photostability and biocompatibility. Their applications as two-photon excitation fluorescence probes for cell membrane imaging have been demonstrated in three different cell lines with excellent imaging contrast. These results demonstrated that these polymer NPs hold great potentials as excellent two-photon excitation fluorescence probes in various biological applications.</description><identifier>ISSN: 1944-8244</identifier><identifier>EISSN: 1944-8252</identifier><identifier>DOI: 10.1021/acsami.5b00223</identifier><identifier>PMID: 25764496</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Bupranolol - analogs & derivatives ; Bupranolol - chemistry ; Cell Line, Tumor ; Cell Membrane - chemistry ; Fluorescent Dyes - chemistry ; Humans ; Molecular Imaging - instrumentation ; Molecular Imaging - methods ; Nanoparticles - chemistry ; Photons ; Polymers - chemistry</subject><ispartof>ACS applied materials & interfaces, 2015-04, Vol.7 (12), p.6754-6763</ispartof><rights>Copyright © 2015 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a330t-d04021ccd7e3059e97915edb6479f8a9e772bc786b1bdd9d387b12abe8ad6d4c3</citedby><cites>FETCH-LOGICAL-a330t-d04021ccd7e3059e97915edb6479f8a9e772bc786b1bdd9d387b12abe8ad6d4c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acsami.5b00223$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acsami.5b00223$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25764496$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Peng</creatorcontrib><creatorcontrib>Li, Shuang</creatorcontrib><creatorcontrib>Jin, Yaocheng</creatorcontrib><creatorcontrib>Qian, Linghui</creatorcontrib><creatorcontrib>Gao, Nengyue</creatorcontrib><creatorcontrib>Yao, Shao Q</creatorcontrib><creatorcontrib>Huang, Fei</creatorcontrib><creatorcontrib>Xu, Qing-Hua</creatorcontrib><creatorcontrib>Cao, Yong</creatorcontrib><title>Red-Emitting DPSB-Based Conjugated Polymer Nanoparticles with High Two-Photon Brightness for Cell Membrane Imaging</title><title>ACS applied materials & interfaces</title><addtitle>ACS Appl. Mater. Interfaces</addtitle><description>New red-emitting conjugated polymers have been successfully synthesized by incorporating classical two-photon absorption (TPA) units, electron-rich units, and a small amount of electron-deficient units along the polymer backbones. Water-dispersible nanoparticles (NPs) based on these polymers were also fabricated for applications in two-photon excitation fluorescence imaging of cell membrane. Through optimization of the polymer/matrix mass ratio and the initial feed concentration of the polymer solution, a high quantum yield (QY) of 24% was achieved for the red-emitting NPs in water. TPA cross section and two-photon action cross section values of these polymers at 750 nm reached up to 1000 GM and 190 GM per repeat unit in aqueous media, 2.5 × 105 GM and 4.7 × 104 GM per NP, respectively. Furthermore, these NPs displayed excellent photostability and biocompatibility. Their applications as two-photon excitation fluorescence probes for cell membrane imaging have been demonstrated in three different cell lines with excellent imaging contrast. These results demonstrated that these polymer NPs hold great potentials as excellent two-photon excitation fluorescence probes in various biological applications.</description><subject>Bupranolol - analogs & derivatives</subject><subject>Bupranolol - chemistry</subject><subject>Cell Line, Tumor</subject><subject>Cell Membrane - chemistry</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Humans</subject><subject>Molecular Imaging - instrumentation</subject><subject>Molecular Imaging - methods</subject><subject>Nanoparticles - chemistry</subject><subject>Photons</subject><subject>Polymers - chemistry</subject><issn>1944-8244</issn><issn>1944-8252</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kElPwzAQhS0EYilcOSIfEVKK7TiLj7SURWKpoJwjO560qZK42I4q_j1GKdw4zWj0zZs3D6FzSsaUMHotSyfbepwoQhiL99AxFZxHOUvY_l_P-RE6cW5NSBozkhyiI5ZkKeciPUb2DXQ0a2vv626Jb-fvk2giHWg8Nd26X0of2rlpvlqw-EV2ZiOtr8sGHN7WfoUf6uUKL7Ymmq-MNx2e2DDwHTiHK2PxFJoGP0OrrOwAP7ZyGa6cooNKNg7OdnWEPu5mi-lD9PR6_zi9eYpkHBMfacLDg2WpM4hJIkBkgiagVcozUeVSQJYxVWZ5qqjSWug4zxRlUkEudap5GY_Q5aC7seazB-eLtnZlcBS8mN4VNE0FT1ie5AEdD2hpjXMWqmJj61bar4KS4ifnYsi52OUcFi522r1qQf_hv8EG4GoAwmKxNr3twqv_qX0D1jyIyQ</recordid><startdate>20150401</startdate><enddate>20150401</enddate><creator>Liu, Peng</creator><creator>Li, Shuang</creator><creator>Jin, Yaocheng</creator><creator>Qian, Linghui</creator><creator>Gao, Nengyue</creator><creator>Yao, Shao Q</creator><creator>Huang, Fei</creator><creator>Xu, Qing-Hua</creator><creator>Cao, Yong</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150401</creationdate><title>Red-Emitting DPSB-Based Conjugated Polymer Nanoparticles with High Two-Photon Brightness for Cell Membrane Imaging</title><author>Liu, Peng ; Li, Shuang ; Jin, Yaocheng ; Qian, Linghui ; Gao, Nengyue ; Yao, Shao Q ; Huang, Fei ; Xu, Qing-Hua ; Cao, Yong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a330t-d04021ccd7e3059e97915edb6479f8a9e772bc786b1bdd9d387b12abe8ad6d4c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Bupranolol - analogs & derivatives</topic><topic>Bupranolol - chemistry</topic><topic>Cell Line, Tumor</topic><topic>Cell Membrane - chemistry</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Humans</topic><topic>Molecular Imaging - instrumentation</topic><topic>Molecular Imaging - methods</topic><topic>Nanoparticles - chemistry</topic><topic>Photons</topic><topic>Polymers - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Peng</creatorcontrib><creatorcontrib>Li, Shuang</creatorcontrib><creatorcontrib>Jin, Yaocheng</creatorcontrib><creatorcontrib>Qian, Linghui</creatorcontrib><creatorcontrib>Gao, Nengyue</creatorcontrib><creatorcontrib>Yao, Shao Q</creatorcontrib><creatorcontrib>Huang, Fei</creatorcontrib><creatorcontrib>Xu, Qing-Hua</creatorcontrib><creatorcontrib>Cao, Yong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>ACS applied materials & interfaces</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Peng</au><au>Li, Shuang</au><au>Jin, Yaocheng</au><au>Qian, Linghui</au><au>Gao, Nengyue</au><au>Yao, Shao Q</au><au>Huang, Fei</au><au>Xu, Qing-Hua</au><au>Cao, Yong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Red-Emitting DPSB-Based Conjugated Polymer Nanoparticles with High Two-Photon Brightness for Cell Membrane Imaging</atitle><jtitle>ACS applied materials & interfaces</jtitle><addtitle>ACS Appl. Mater. Interfaces</addtitle><date>2015-04-01</date><risdate>2015</risdate><volume>7</volume><issue>12</issue><spage>6754</spage><epage>6763</epage><pages>6754-6763</pages><issn>1944-8244</issn><eissn>1944-8252</eissn><abstract>New red-emitting conjugated polymers have been successfully synthesized by incorporating classical two-photon absorption (TPA) units, electron-rich units, and a small amount of electron-deficient units along the polymer backbones. Water-dispersible nanoparticles (NPs) based on these polymers were also fabricated for applications in two-photon excitation fluorescence imaging of cell membrane. Through optimization of the polymer/matrix mass ratio and the initial feed concentration of the polymer solution, a high quantum yield (QY) of 24% was achieved for the red-emitting NPs in water. TPA cross section and two-photon action cross section values of these polymers at 750 nm reached up to 1000 GM and 190 GM per repeat unit in aqueous media, 2.5 × 105 GM and 4.7 × 104 GM per NP, respectively. Furthermore, these NPs displayed excellent photostability and biocompatibility. Their applications as two-photon excitation fluorescence probes for cell membrane imaging have been demonstrated in three different cell lines with excellent imaging contrast. These results demonstrated that these polymer NPs hold great potentials as excellent two-photon excitation fluorescence probes in various biological applications.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>25764496</pmid><doi>10.1021/acsami.5b00223</doi><tpages>10</tpages></addata></record> |
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| subjects | Bupranolol - analogs & derivatives Bupranolol - chemistry Cell Line, Tumor Cell Membrane - chemistry Fluorescent Dyes - chemistry Humans Molecular Imaging - instrumentation Molecular Imaging - methods Nanoparticles - chemistry Photons Polymers - chemistry |
| title | Red-Emitting DPSB-Based Conjugated Polymer Nanoparticles with High Two-Photon Brightness for Cell Membrane Imaging |
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