TnMax — a versatile mini-transposon for the analysis of cloned genes and shuttle mutagenesis

A series of Tn7721-based mini-transposons (TnMax) has been developed which are suitable for the targeting of cloned genes, shuttle mutagenesis, identification of protein export signals and the rescue of chromosomal markers. TnMax mini-Tn consist of two 38-bp inverted repeats (IR) flanking a resoluti...

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Veröffentlicht in:	Gene 1993-08, Vol.130 (1), p.23-31
Hauptverfasser:	Haas, Rainer, Kahrs, Andreas F., Facius, Dirk, Allmeier, Helmut, Schmitt, Rüdiger, Meyer, Thomas F.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biological and medical sciences Biotechnology catGC Cloning, Molecular DNA Mutational Analysis - methods DNA Repair DNA Transposable Elements - genetics DNA, Bacterial - analysis Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes, Bacterial Genetic engineering Genetic technics Genetic Vectors Methods. Procedures. Technologies Molecular Sequence Data Mutagenesis, Insertional - methods natural transformation Neisseria gonorrhoeae - genetics Oligonucleotides orifd Promoter Regions, Genetic Ptrcpromoter shuttle-transposon Site specific mutagenesis Tn phoA Tn1721
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